Potencial antioxidante, mineral, inibitório de enzimas α-amilase e lipoxigenase e composição centesimal de espécies da família Apiaceae

Eryngium foetidum L., Eryngium cf. campestre and Coriandrum sativum L. are Apiaceae family vegetable appreciated due to its peculiar flavor and consumed mainly in the north and northeast of Brazil. The vegetables are rich in protein, vitamins, fiber, minerals, total phenolics and other essential bioactives for a balanced health. Nevertheless, many vegetables are falling into disuse by the population, instead of processed foods. The rescue consumption of these species is very important, aiming at their nutritional, therapeutic and antioxidant benefits. In this study, was quantified the levels of total phenolic, flavonoids and dihidroflavonoides by molecular absorption spectrophotometry in the ultraviolet. The total antioxidant capacity was also evaluated using five methodologies of in vitro assays: test Total Antioxidant Capacity (TAC), scavenging of DPPH and ABTS radical, Power Reducing and Power Chelating. It was also evaluated the power inhibitor of α-amylase and lipoxygenase extracts. All species showed significant levels of total phenolics, flavonoids and dihidroflavonoides in its composition. All treatments showed antioxidant activity of 50% except the sheets of E. cf. campestre, C. sativum and bracts of E. foetidum in DPPH and bracts of E. foetidum in ABTS. All treatments also exhibited 50% inhibition activity of the enzyme lipoxygenase.In α-amylase only the leaves of E. cf. campestre and C. sativum showed IC50. It was evaluate the phytochemical composition, aiming to meet the nutritional potential of Apiaceae family vegetables, called unconventional: Eryngium foetidum L., Eryngium cf. campestre; and conventional: Coriandrum sativum L. At the centesimal composition analysis Coriandrum sativum L. presented the highest levels of protein. The leaves of Eryngium foetidum L. exhibited higher values than other species in dietary fiber, while Eryngium cf. campestre detach with superior results in lipids. About the analyzed minerals, the leaves of Eryngium cf. campestre expressed results superior to the other in N, Ca, Mg, S and Cu. The amount of iron highlighted in sheets of E. foetidum, whereas P, K, Mn, Zn and B were most significant on leaves of C. sativum. It was concluded that the levels of total phenolic compounds found in these vegetables, characterize them for its high potential in the antioxidant and inhibition of lipoxygenase and α-amylase enzymes. Their protein and mineral levels classify them as species that can be used as a nutritional source in the preparation of other foods and may their regular consumption bring benefit to human health.

Acidovorax citruli: genetic analyses and protocol for its detection in seeds

Bacterial fruit blotch of cucurbits (BFB), caused by the seed borne Gramnegative bacterium Acidovorax citrulli is a serious threat to cucurbit industry worldwide. Since late 1980`s after devastating outbreaks in watermelon fields in southern United States, BFB has spread worldwide and has been reported in other cucurbit crops such as melon, pumpkin, cucumber and squash. To date, there is evidence for the existence of at least two genetically and pathogenically distinct populations of A. citrulli. In Brazil, the first report of BFB was in 1991, in a watermelon field in São Paulo. Although widespread in the country, BFB has been a major problem to melon production. More precisely, BFB has caused significant yield losses to melon production in northeastern Brazil, which concentrates > 90% of the country`s melon production. Despite the management efforts and the recent advances in A. citrulli research, BFB is still a continuous threat to the cucurbit industry, including seed producers, growers and transplant nurseries. To better understand the population structure of A. citrulli strains in Brazil, and to provide a basis for the integrated management of BFB, we used pulsed-field gel electrophoresis (PFGE), multilocus sequence analysis (MLSA) of housekeeping and virulence-associated genes and pathogenicity tests on different cucurbit seedlings to characterize a Brazilian population of A. citrulli strains from different hosts and regions. Additionally, we conducted for the first time a comparative analysis of the A. citrulli group I and II population at genomic level and showed that these two groups differ on their genome sizes due to the presence of eight DNA segments, which are present in group II and absent in group I genomes. We also provide the first evidence to suggest that temperature might be a driver in the ecological adaptation of A. citrulli populations under nutrient-rich or -depleted conditions. Finally, in order to improve the routine detection of A. citrulli on melon seedlots, we designed a new primer set that is able to detect the different Brazilian haplotypes, thus minimizing the risk of false-negatives on PCR-based seed health testing.

Componentes principais na elaboração de índices de provas de desempenho em bovinos

The principal component analysis assists the producers in making decision of which evaluated features must be maintained in performance tests indexes, according to the variation present in these animals evaluated. The objective in this study was to evaluate a set of characteristics measured in a performance test in semifeedlot cattle of the Simmental and Angus breeds, by means principal component analysis (PC), aim to identify the features that represent most of the phenotypic variation for preparation of indexes. It was used data from 39 Angus and 38 Simmental bulls from the Santa Éster farm, located in Silvianópolis - MG. The performance test period was from october 2014 to february 2015. The features evaluated in the test were: final weight (FW), average daily gain weight (GW), respiratory rate (RR), haircoat temperature (HT) and rectal (RT), hair number (HN), hair length (HL), hair thickness (HT), muscularity (MUSC), racial characteristics, angulation, reproductive and balance (BAL), height of the front and back, width and length of croup, body length, depth and heart girth, subcutaneous fat thickness and rump (FTR), loin eye area and marbling (MAR). It was used PRINCOMP from SAS program for procedure the PC analysis. It was found that of the 27 features evaluated, the first four PC for Simmental breed explained 74% total variation data. The four PC selected with the corresponding weighting coefficients formed the following index: (0.27 * FW) + (0.47 * MUSC) + (0.50 * HL) + (0.39 * HT). Since the characteristics related to the adaptability of great importance for the studied breed, it was decided to keep the index of evidence for the Angus breed, the feature hair number, because there is a feature that presented a great variability and occupied one of the first principal component. Thus, the Angus index was composed by five features, with 79% total variation data, resulting in the following formula: (0.26 * FW) + (0.33 * BAL) + (0.58 * MAR) - (0.43 * FTR) – (0.38 * HN). By the principal component analysis it was possible to minimize the features number to be evaluated on performance tests from that farm, making the animal selection rapidly and accurate.

Modelagem do crescimento e produção em povoamentos desbastados de Eucalyptus grandis

The aim of the study was to develop a system of growth and yield models for thinned stands of Eucalyptus spp.; and to assess the behavior of the growth in scenarios with 10% decrease or increase in rainfall. The probability distribution functions Weibull 2 and 3 parameters and Johnson SB for different methods were fitted. Correlation between the fitted parameters with age was evaluated. Dominant height growth behavior was evaluated to check if thinned stand changes its growth when compared to a non-thinned stands. The stand variables dominant height and basal area were projected and simultaneously predicted and projected, respectively. Individual tree equations were fitted, which were fitted as functions of stand level variables in order to decrease the error propagation. R software was used to fit all the proposed models and consequently all the fitted models were evaluated by their parameters significance (F-test) and graphs of predicted values in relation to the observed values around the 1:1 line. Thus, the prognosis system was made by two ways, first one using the full data set, and for the second one the dataset was restricted at age 7.5. Increase and decrease in 20% of rainfall were assessed by updating the site index function. Method of moments was the most precise to describe the diameter distribution for every age in eucalyptus stands for Johnson SB and Weibull 2 parameters pdfs. When observed for each pdf the correlation for their fitted parameters with age, we noticed that shape parameters for a thinned stand were no longer correlated with age, differently of non-thinned stands. Thus, thinning effect was accounted in the basal area prediction and projection modeling. This result emphasized the necessity of applying the Parameter Recovery method in order to assess differences and capture the right pattern for thinned and non-thinned stands in the future. Dominant height was not influenced by thinning intensity. Therefore the fitted Chapman-Richards model did not account for a stand being thinned or not. All the fitted equations behaved with good precision, no matter using full or precocious dataset. The prognosis system using full and/or precocious date set was evaluated for when using Parameter Recovery method for Sb and Weibull pdfs, and by then, graphical analysis and precision statistics showed appropriated results. Finally, the increase or decrease in rainfall regime were observed for eucalyptus stand yields and we may notice how important is to observe this effect, since the growth pattern is strictly affected by water.

Arginina para matrizes suínas primíparas em lactação

With the emergence of new genetic lines due to intense breeding improvement on swine production in recent years, there is the need to adapt more accurately diets for the current sows, which have higher nutritional demands. The use of functional amino acids aimsto optimize the sows production and among these amino acids arginine has excelled. Arginine is involved in several important metabolic pathways, for example, it serves as a substrate forsynthesis of protein, creatine, nitric oxide, polyamines, citrulline, agmatine, ornithine, proline, and glutamate. It also helps to stimulate the secretion of some hormones such as insulin, prolactin, and growth hormone.As arginine plays such important roles, its supplementation has been suggested in lactation feed once it may enhance the development of the mammary gland and milk nutritional profile, thus, providing a better piglet development.Thus, the objective was to evaluate the effect of lactation feed supplementation with L-Arginine on the productive performance of primiparoussows and their respective litter.One hundred forty sows from the same genetic lineage on a commercial farm, located in the city of Oliveira, MG were used in this study, in a completely randomized design with five treatments: control diet without amino acid supplementation and four diets with increasing levels of L-Arginine supplementation (containing 98.5% purity) - 0.5, 1.0, 1.5, and 2.0%. Each treatment hadtwenty-eight swine sows, and the experimental unit was the sowand its litter.It was used ‘on top’ amino acid supplementation.All data was submitted to variance analysis using the SAEG Software: version 9.1 (SAEG, 2005).The data relating to days of lactation were compared by Tukey test (5%). L-Arginine supplementation levels in lactation feed did not influence (P>0.05) average daily feed intake, body condition variables, and blood parameters of the sows (urea, creatinine, and non-esterified fatty acids) as well as it did not affect the dry matter, crude protein, and amino acid profile of milk and the litter performance. There was effect (P<0.05) of days of lactation on the percentage of crude protein and amino acids in milk, which reduced througout the days of lactation. The L-Arginine supplementation on the lactation diet at levels of 0.5, 1.0, 1.5, and 2.0% did not influence the sow and its respective litter performance.

Inovação do método MIA-QSAR: aplicação para doenças tropicais negligenciadas

Multivariate image analysis applied to the quantitative structure-activity relationships (MIA-QSAR) is a 2D QSAR technique that has been presenting promising outcomes for the development of new drug candidates, due to its simplicity, rapidity and low cost. In this way, the present study aims at introducing, consolidating and improving the new dimensions named aug-MIA-QSAR and aug-MIA-QSARcolor, as well as applying them to the study of neglected diseases, in order to obtain new drug targets using chemico-biological interpretation of the MIA molecular descriptors. Four compound data sets with experimental bioactivities against Chagas disease, malaria, dengue and schistosomiasis were evaluated using three approaches: MIA-QSARt, aug-MIA-QSAR and aug-MIA-QSARcolor. In general, representations of atoms as spheres with different colors and sizes proportional to the corresponding van der Waals radii (aug-MIA approaches) improved the predictive ability and interpretability in all data sets. The use of colors proportional to the Pauling´s electronegativity showed that MIA descriptors are capable of identifying periodic properties relevant for the studied activity. Finally, solid colors instead of spotlighted atoms allowed a correct identification of atoms by means of pixel values in the studies for malaria, dengue and schistosomiasis, which were, subsequently, useful for the chemical interpretation related to the bioactivity. It can be inferred that semicarbazones and thiosemicarbazones derivative with a tri-substituted ring in R1 group and a trifluoro methyl group in the R 3 position instead of a chlorine antitripanossoma resulted in higher activity. The antimalarial activity of quinolon-4(1H)imines can be improved if: 1) R1 and R2 are electron donor groups, 2) R3 has long aminoalkyl chains, and 3) R4 possesses substituents with big atomic volume. In the study for dengue, it was found that tetrapeptides with unbranched small size amino acids in the A1 and A4 positions can increase the substrate affinity (Km) to the NS3 protein, and when in A1 and A2 positions, the substrate cleavage rate (kcat). On the other hand, acidic amino acids in the A2 and A4 positions were found to be related with low substrate affinity to the NS3 protein and when present in A1, with low substrate cleavage rate. Finally, the presence of metoxy substituents in R1 (or R2) and R5 in the neolignan backbone can favor their antischistosomal activity.

Compartimentos de K, Ca e Mg em solos e sua relação com o crescimento inicial, fisiologia e produtividade do eucalipto

Forestry has grown in a continuous and accelerated manner in Brazil, constituting a strategic activity for the generation of employment, income and tributes, favoring social and economic development of Brazilian agribusiness. The objectives of this study were: (1) evaluate the contents of K, Ca and Mg in the reserve compartments, non-interchangeable, interchangeable, available and the speed of its release, its correlations and its effects over productivity (annual average increment – AAI) of eucalyptus plantations, in forest sites cultivated in soils of the state of Rio Grande do Sul; (2) evaluate the initial growth, nutrition and physiological aspects of eucalyptus plants, cultivated with and without the addition of mineral sources of potassium (K), calcium (Ca) and magnesium (MG), in soils obtained from forest sites in the state of Rio Grande do Sul. In the first study, contents of K, Ca and Mg were evaluated in sulfuric digestion extract, boiling nitric acid, ammonium chloride, Mehlich-1 (only K), potassium chloride (Ca and Mg), as well as the release speed of these nutrients in the soil. In the second study, growth variables, nutritional aspects, photosynthetic rate (A) and transpiration rate of the plants (E) grown in distinct soils were evaluated under controlled conditions. The contents of K, Ca and Mg varied between compartments and depths in the studied soil classes, with the highest proportions found in the reserve compartment, indicating the importance of this compartment for the supplement of these nutrients at average and long terms. The great majority of K, Ca and Mg compartments presented significant correlations between each other, showing the dependence between them and the importance of evaluating the contents of these nutrients in the different compartments to adapt the nutritional management of the plants to each soil class, and to obtain continuous productions, minimizing the negative effects to the environment. Plants cultivated in soils that present larger reserves, availability and K, Ca and Mg release kinetics, presented similar height (H), stem diameter (SD) and shoot dry mass (SDM), with or without fertilization with K, Ca and Mg. The plants presented higher leaf content and accumulation of K in all soils fertilized with K, Ca and Mg.

Biofortificação com zinco, selênio e ferro, e biodisponibilidade de ferro em cultivares de feijoeiro-comum

The common bean (Phaseolus vulgaris L.), a staple food in nutritional diet of Brazilians and populations in developing countries, is a nutritionally rich legume with potential for biofortification. Approximately one third of the world population suffers from nutritional deficiencies, being necessary to increase the nutrient content in vegetables, especially iron (Fe), selenium (Se) and zinc (Zn), which are important micronutrients for plants and human health. In this context, three studies were carried out aiming to evaluate the potential of common bean cultivars to biofortification with Fe, Se and Zn, and verify the interaction between these minerals and iron bioavailability, in order to contribute to increased nutritional quality of grains, reducing the micronutrients deficiency and improving human health. In the first study, experiments were conducted in a greenhouse, with ten common bean cultivars in nutrient solution under different treatments with Fe, Se and Zn. The plant growth and the mineral content of the beans were evaluated in addition to verify the influence of polyphenol and phytate levels on Fe bioavailability in grains fortified with Zn and Se. The evaluated beans cultivars have proved promising for simultaneous biofortification with these nutrients without greatly affecting Fe bioavailability. In the second study, the aim was evaluate the interaction between Fe, Se and Zn in cultivars consumed in Brazil or in USA. Gene expression and root microscopy analysis were performed in order to understand the positive effect of Zn supply on the Fe uptake by roots. The expression of genes related to the transport and uptake of Fe and Zn did not clearly explain the influence of Zn in Fe nutrition. The roots microscopy and the evaluation of nutrient solutions used showed that, in the presence of Zn, there was Fe accumulation in epidermis of the roots and not in the vascular system, prone to be precipitated when it goes through the root membrane. In the latest study, a field experiment was conducted to evaluate the effect of Zn fertilization via soil and foliar, in the content and accumulation of Fe and Zn in grains and in the yield of common bean cultivars, in addition to verify the amount of these micronutrients supplied by biofortified beans. The fertilization with Zn did not affect the yield, but provided high levels of this nutrient in grains of the cultivars analyzed, representing 27% of the recommended daily intake of Zn. The higher Fe content in beans, obtained when there was no application of foliar Zn, supplies 56% of the daily requirement of Fe.

Metodologias para a avaliação do potencial fisiológico de sementes de tabaco

Despite tobacco being a culture propagated by seeds, there is little information concerning tests that allow the distinction of similar germination lots in different levels of vigor. The diversity of cultivars available in the market, and a few peculiarities of the species, such as uneven maturation of the flowers, fruits and seeds, small size and seed dormancy, are considered obstacles for obtaining lots of tobacco of high physiological potential. Thus, this research was developed with the objective of adapting feasibility and vigor tests for evaluating the physiological potential of tobacco seed lots. We used nine lots of tobacco seeds of cultivar CSC 447 and nine lots of seeds of cultivar BAT 2101, belonging to variety groups Virginia and Burley, respectively. Initially, germination test was conducted to characterize the profile of the lots. For determining the feasibility and vigor of the tobacco seeds, germination tests were conducted in distinct temperatures, controlled emergence conditions, electric conductivity, artificial aging and in tetrazolium. For determining the isoenzymatic marker for seed quality, analyses were conducted with enzymes catalase, esterase, malate dehydrogenase and alcohol dehydrogenase. In conclusion, the emergence tests at 25oC and artificial aging at 41oC for 72 hours, are efficient in discriminating the lots of tobacco seeds in different levels of vigor. The electric conductivity and germination tests in different temperatures have distinct responses in relation to the genotype of the tobacco seeds. The tetrazolium test using the methodology with pre-conditioning in 3.5% sodium hypochlorite solution and subsequent emersion in 1.0% tetrazolium solution for 18 hours is efficient for the quick evaluation of the feasibility of tobacco seeds. The analysis of the profiles of enzymes catalase, esterase, malate dehydrogenase and alcohol dehydrogenase is efficient as markers for tobacco seed quality.

Modelos aplicados en Fitopatología

Dentro de las diversas áreas de la Fitopatología, la Epidemiología es la que posee un mayor potencial para el uso de modelos, porque esta ciencia es responsable de la descripción de las enfermedades a nivel de poblaciones y comunidades. Como las poblaciones están formadas generalmente por un número de individuos, es imposible e indeseable descubrir qué ocurre con individuos aislados. Los epidemiólogos precisan de alguna manera representar lo que sucede en las poblaciones de plantas, frente a los patógenos, en diferentes situaciones de ambiente. Haciendo una revisión de literatura, se verificó que hay dos caminos por los cuales los epidemiólogos estudian las enfermedades en poblaciones de plantas a través de modelos matemáticos, y de modelos estadísticos.

Identificação do enfermeiro de saúde pública na força de trabalho de Enfermagem de Saúde Pública no Departamento Regional de Saúde-6 de Ribeirão Preto, SP (Brasil)

Objetivou-se identificar o enfermeiro na força de trabalho de Enfermagem em Saúde Pública, no Departamento Regional de Saúde - 6 de Ribeirão Preto da Secretaria de Estado da Saúde, destacando e analisando alguns aspectos que o caracterizam. Utilizou-se, como fonte primária e direta de coleta de dados, questionário contendo dados de identificação, nível de formação, progressão funcional, funções, expectativas futuras quanto ao exercício profissional e filiação a órgãos de classe. O total do grupo estudado foi de 35 indivíduos do sexo feminino, predominando: grupo etário de 20 a 30 anos (40,0%); casados (51,5%); tempo de serviço entre zero e 15 anos (77,2%). Com referência à sua interproporcionalidade com as demais categorias, as enfermeiras se distribuíam nas seguintes relações: 1 enfermeira/13,5 pessoal auxiliar e 1 enfermeira/9,1 médicos. Quanto à formação profissional, 71,5% realizaram habilitação em Enfermagem em Saúde Pública e 8,6% Especialização em Saúde Pública. Não havia para as enfermeiras carreira nem quadro e seus vencimentos estavam entre 2 e 7 salários mínimos e apenas uma com 10. A função administrativa é exercida por todas as enfermeiras com predomínio de freqüência entre as inspetoras e de CS, 68,5%. As enfermeiras sentem-se preparadas para participar dos programas de assistência primária e 84,3% delas pretendem permanecer em exercício até sua aposentadoria.

Federalismo fiscal no Brasil: arrecadação, transferências e disponibilidades (1988 - 2000)

Este trabalho tem por objetivo identificar e analisar as mudanças ocorridas no cenário do federalismo fiscal-tributário brasileiro, no período entre a promulgação da Constituição Federal de 1988 e o encerramento do exercício financeiro de 2000. O referencial teórico está fundamentado na teoria das finanças públicas. Os dados foram obtidos junto a Secretaria da Receita Federal, a Secretaria do Tesouro Nacional e ao Instituto Brasileiro de Geografia e Estatística. Os resultados apontam para uma reversão parcial do padrão de descentralização observado no triênio posterior a promulgação da Constituição de 1988. As disponibilidades da União foram parcialmente reconstituídas, com ênfase nas receitas vinculadas ao financiamento da seguridade social. No âmbito subnacional os estados perderam participação relativa na distribuição das disponibilidades tributárias e os municípios afirmaram a posição obtida no ordenamento constitucional de 1988.

Câmbio e arrecadação municipal no Brasil: uma análise empírica de 2004 a 2007

Este trabalho busca testar empiricamente a associação estatística entre taxa de câmbio real local e arrecadação dos municípios brasileiros para o período de 2004 a 2007. O trabalho apresenta evidências empíricas sobre a relação positiva, mas heterogênea entre a taxa cambial e o montante arrecadado de IPTU nos municípios do Brasil no período de 2004 a 2007. Encontramos que a depreciação do real em relação ao dólar em um real (comparativamente a dois anos atrás) está associado a um aumento de R$ 4,65 o montante arrecadado em média por habitante através do IPTU. Não encontramos efeito da taxa de câmbio real sobre a arrecadação do ISS de forma direta.

Eficiência na arrecadação de tributos: análise através de uma fronteira estocástica de custo aplicada as delegacias da Receita Federal do Brasil

O presente estudo pretende avaliar o desempenho das Delegacias da Receita Federal através do estabelecimento de uma fronteira de eficiência paramétrica baseada nos custos, utilizando para tal um modelo estocástico que divide o ruído em dois componentes, sendo um aleatório e outro proveniente da ineficiência de cada unidade. O trabalho terá por base dados relativos aos anos de 2006 e 2008 em uma análise em corte transversal e visa avaliar a política pública de unificação dos órgãos estatais responsáveis pela arrecadação de tributos em nível Federal, a Secretaria da Receita Federal (SRF) com a Secretaria da Receita Previdenciária (SRP), ocorrida através da lei 11.457 de 16 de março de 2007. O objetivo principal da pesquisa é determinar se as unidades descentralizadas da Receita Federal, notadamente as Delegacias da Receita Federal estão operando com eficiência, na tarefa de arrecadar tributos, em função dos recursos colocados a disposição para execução de suas atividades. Na presente pesquisa o produto da unidade a ser avaliado é a arrecadação, dentre as inúmeras atividades realizadas pelo órgão, no sentido de proporcionar ao Estado recurso para implantação de Políticas Públicas. O resultado encontrado indica que as regiões onde existe um grande número de empresas optantes pelo regime de tributação do SIMPLES, bem como as que possuem em sua jurisdição empresas consideradas DIFERENCIADAS pelo seu porte, provocam um aumento nos custos das Delegacias. As unidades que se encontram nas capitais dos Estados melhoraram o seu desempenho após a unificação. Além disso, uma proporção maior de Auditores Fiscais dentro da Delegacia em relação ao total de servidores reduz a ineficiência. O trabalho espera contribuir na avaliação desse novo modelo de gestão implantado na administração tributária federal no país.

Um estudo sobre a distribuição das transferências condicionais no setor de saúde no Brasil

Este trabalho estuda empiricamente alguns fatores determinantes das transferências intergovernamentais na área da saúde no Brasil. Em especial, discute e investiga a existência de uma relação positiva entre arrecadação municipal e o recebimento de recursos federais através do Sistema Único de Saúde (SUS) pelos municípios brasileiros. Como a legislação do SUS atrela gastos dos municípios obrigatórios em saúde a uma parcela de sua arrecadação, o fenômeno também pôde ser investigado à luz da teoria do flypaper effect. A análise empírica foi realizada através da estimação de modelos em painel com efeitos fixos. Para tanto, utilizou-se dados dos municípios brasileiros no período de 2002 a 2010, obtidos, em sua maioria, através do Departamento de Informações do SUS (DATASUS) e do Sistema de Informações sobre Orçamentos Públicos em Saúde (SIOPS), ambos do Ministério da Saúde. Os resultados apontam que a arrecadação municipal exerce, de fato, um impacto positivo e significante sobre o recebimento de transferências. Considerando o aspecto redistributivo, essa relação pode não ser desejável. Argumentamos sobre a possibilidade de que os recursos repassados pelo SUS não sejam suficientes, de modo que uma contrapartida financeira dos governos locais seria necessária para cobrir os custos dos programas e serviços em sua totalidade. Foram identificados também indícios de que pode haver fatores políticos influenciando o recebimento de recursos do SUS.