Cortactin mediated morphogenic cell movements during zebrafish (Danio rerio) gastrulation

Cell migration is essential to direct embryonic cells to specific sites at which their developmental fates are ultimately determined. However, the mechanism by which cell motility is regulated in embryonic development is largely unknown. Cortactin, a filamentous actin binding protein, is an activator of Arp2/3 complex in the nucleation of actin cytoskeleton at the cell leading edge and acts directly on the machinery of cell motility. To determine whether cortactin and Arp2/3 mediated actin assembly plays a role in the morphogenic cell movements during the early development of zebrafish, we initiated a study of cortactin expression in zebrafish embryos at gastrulating stages when massive cell migrations occur. Western blot analysis using a cortactin specific monoclonal antibody demonstrated that cortactin protein is abundantly present in embryos at the most early developmental stages. Immunostaining of whole-mounted embryo showed that cortactin immunoreactivity was associated with the embryonic shield, predominantly at the dorsal side of the embryos during gastrulation. In addition, cortactin was detected in the convergent cells of the epiblast and hypoblast, and later in the central nervous system. Immunofluorescent staining with cortactin and Arp3 antibodies also revealed that cortactin and Arp2/3 complex colocalized at the periphery and many patches associated with the cell-to-cell junction in motile embryonic cells. Therefore, our data suggest that cortactin and Arp2/3 mediated actin polymerization is implicated in the cell movement during gastrulation and perhaps the development of the central neural system as well.

Haematopoietic lineage cell-specific protein 1 (HS1) promotes actin-related protein (Arp) 2/3 complex-mediated actin polymerization

HS1 (haematopoietic lineage cell-specific gene protein 1), a prominent substrate of intracellular protein tyrosine kinases in haematopoietic cells, is implicated in the immune response to extracellular stimuli and in cell differentiation induced by cytokines. Although HS1 contains a 37-amino acid tandem repeat motif and a C-terminal Src homology 3 domain and is closely related to the cortical-actin-associated protein cortactin, it lacks the fourth repeat that has been shown to be essential for cortactin binding to filamentous actin (F-actin). In this study, we examined the possible role of HS1 in the regulation of the actin cytoskeleton. Immunofluorescent staining demonstrated that HS1 co-localizes in the cytoplasm of cells with actin-related protein (Arp) 2/3 complex, the primary component of the cellular machinery responsible for de novo actin assembly. Furthermore, recombinant HS1 binds directly to Arp2/3 complex with an equilibrium dissociation constant (K-d) of 880 nM. Although HS1 is a modest F-actin-binding protein with a Kd of 400 nM, it increases the rate of the actin assembly mediated by Arp2/3 complex, and promotes the formation of branched actin filaments induced by Arp2/3 complex and a constitutively activated peptide of N-WASP (neural Wiskott-Aldrich syndrome protein). Our data suggest that HS1, like cortactin, plays an important role in the modulation of actin assembly.

旋转机械全息序列相似性匹配故障诊断方法

针对全息诊断分辨率低影响旋转机械故障诊断质量和自动化水平的问题,将时间序列相似性匹配的基本概念和方法引入故障诊断应用中,结合全息诊断信息融合分析旋转机械振动全貌的思想,定义了全息序列及其相似性度量模型,用类时间轴上的多维序列表征转子系统振动全貌,进而利用采用近似三角不等式与B+树结合剪枝策略的全息序列相似性匹配算法实现故障诊断。实验结果表明,该方法能够实现高质量的故障自动分类识别。

基于时间序列数据挖掘的旋转机械预诊断方法研究

基于状态的维护(CBM, Condition Based Maintenance)是近年来新兴的一种设 备维护策略，它的基本理念是在机械设备需要维护的时候才对其进行维护，强调 维护要及时、准确和经济。采用这种维护策略，能够提高工业生产的安全性和可 靠性，系统地降低企业运营成本。 机械设备状态预诊断是实现CBM 的核心支撑技术，对其进行深入研究，对推 动CBM 的发展具有重要意义。但是，由于相关研究起步不久，目前预诊断技术还 未能得到很好的实现，研究人员有必要不断地尝试各种新的有效方法来更好地解 决这一问题，加快其实现方法与技术应用的成熟进程。基于此，本文从数据挖掘 的角度，探索了机械设备预诊断新的解决方法和途径，深入研究和探讨了基于时 间序列数据挖掘的旋转机械预诊断方法。本文的主要工作包括： 1. 结合CBM 的基本理念和应用实际的需求，对机械设备状态预诊断的基本 内涵进行了系统分析。将状态评估、故障预测和剩余有效使用寿命预测三个预诊 断基本功能进一步抽象，提出了包含特征提取、状态预测和模式匹配三个子问题 的预诊断一般流程模式。在详细分析机械设备状态预诊断理论方法和应用技术研 究现状的基础上，提出了预诊断技术研究的发展趋势及各子问题的研究侧重点。 并对利用时间序列数据挖掘这一理论方法解决机械设备状态预诊断问题的可行性 进行了分析。 2. 针对具有波动频繁、噪声干扰严重等特点的原始振动量时间序列无法直接 用于旋转机械性能状态分析的问题，结合全息诊断信息融合分析旋转机械振动全 貌的思想，提出了全息状态矩阵的概念并给出定义，用类时间轴上的多维序列表 征转子系统振动全貌，以实现振动量时间序列的高级表示，为后续预测与匹配分 类工作提供良好的数据源，同时增强全息诊断的信息检索和知识自动获取的能力。 3. 将旋转机械性能状态预测，归结为旋转机械设备维护应用背景下的一维数 值型时间序列预测问题来进行深入研究。针对现有预测方法长期预测能力较弱， 且自动化水平低的不足，提出了用于旋转机械性能状态预测的ARIMA 动态间隔预 测法。该方法以动态间隔获取时间序列样本建模并预测的策略，提高了ARIMA 模 型用于设备状态长期预测的准确性，并且能够实现建模与预测的自动化，满足CBM 系统的实时性要求。 4. 针对全息状态矩阵表示的旋转机械性能状态特征数据，提出了一种全息状 态矩阵相似性匹配方法。结合旋转机械预诊断领域应用的特点定义了全息状态矩 阵的相似性度量模型，基于全息状态矩阵近似距离三角不等式设计了剪枝搜索策 略，并在此基础上设计了全息状态矩阵相似性高效准确匹配算法，不需要借助专家经验和人工识别确认，在一定阈值范围内能够实现高质量的旋转机械性能状态 相似性匹配。 5. 旋转机械基本振动量特征时间序列具有海量、超高维度、短期波动频繁和 大量噪声等特征，与时间序列数据挖掘传统应用的金融商业领域数据不同，直接 采用传统方法会存在搜索速度大幅度降低的问题。针对这一问题，提出了基于随 机投影的时间序列相似性搜索方法。该方法利用近年来新兴的随机投影统计学降 维法，将原始时间序列集映射到低维空间，并利用R*树进行索引，能够在保持高 准确率的同时，实现旋转机械基本振动量特征时间序列相似性快速搜索。 6. 针对现有机械设备性能状态分类方法不考虑误分类代价的问题，提出了一 种代价敏感直推式旋转机械设备性能状态分类法。该方法将代价敏感分类和直推 式学习的基本思想和理论相结合，采用一种代价敏感的直推式分类机制，实现了 机械设备性能状态的代价敏感分类。该方法在保证较高分类准确率的基础上，明 显地降低了误分类总代价。 7. 基于CBM 的基本理念，设计了旋转机械CBM 系统的基本结构，并以本 文理论方法的研究成果为核心，详细设计了各模块的基本功能和处理逻辑，采用 VC#.net 与Matlab 混合编程的方式开发了一个面向大型旋转机械的CBM 系统原 型，以验证本文机械设备预诊断方法研究成果的可操作性和实用性，为CBM 系统 应用技术研究做出了有益的探索。

智能化工程机械发展战略研究

The intelligent level of construction machinery abroad is described in the article. In association with automation level of domestic construction machinery, developing targets are pointed out. Some plans and schemes in national technical 863 projects are introduced, which may be of reference to domestic enterprises when making developing schedule for organization.

用于AUV收放装置的变幅机构研究

这里主要介绍了变幅机构的理论分析及AUV载体的收放方法,并简要介绍了收放机构的组成情况。

空间冗余度机器人最小关节力矩的轨迹规划

许多工程机械的工作装置属于冗余度机器人的范畴 ,该类机械施工控制自动化所涉及到的轨迹规划问题 ,一直受到人们的重视·采用冗余度机器人运动学的最小关节力矩法 ,对空间4R冗余度机器人的关节轨迹的运动规划进行了分析仿真·实验表明该方法实际可行 ,运动学和动力学的工作特性良好·

锻压机导套对立柱作用反力的分析研究

讨论了锻压机工作时偏心载荷对机架下横梁和立柱应力变化的影响程度，验证了下横梁的断裂原因；详细地讨论了导套筒对立柱作用反力的影响，指出了传统液压机设计方法中假设条件的不足，为该类机械的结构设计和改进提出了新的见解。

汽车零件精段自动生产线机械手设计

在高温、多粉尘、多有害气体的危险环境中,工业机械手的应用就尤为显得重要,我们设计的液压组合式机械手特点是巧妙地使油缸远离手爪,以防油液受热使油的黏度下降。能在振动、油污等条件下稳定和可靠的工作,在锻造水压机生产流水线中,代替人手来搬运,装卸和操作,不但减轻了工人的劳动强度还大大的提高了劳动生产率。

鄂尔多斯盆地环县地区长6-长8沉积相及储层研究

The exploration in recent years shows that the Yanchang Formation in the southwest of Ordos Basin is of great resource potential and good exploration and exploitation prospect. In the thesis ，sedimentary source analysis，sedimentary system，sedimentary microfacies，sandstones distribution and reservoir characteristic are studied and favorable oil area are forecasted in Chang6-Chang8 of Yanchang formation in HuanXian region， by mainly study on the data of field section observation ，core observation, well logging explaination and routine microscope slice identification，scanning Electron Microscope and reservoir analysis of lithology and physical property , Under the guidance of such advanced theories and methods as sedimentology，reservoir sedimentology，lithological oil pool and so on. The stratum of Chang6-Chang8 of Yanchang formation could be divided into pieces of member following the principles that firstly contrasting the big segments, then contrasting the small segments, being controlled by cycle and consulting the thickness etc.And the characteristic of stratum are detailed discussed , respectively. Based on the source direction of the central basin, heavy and light minerals are used to analyse source direction of Chang6 and Chang8 member, in HuanXian area. Research result shows that the source of Chang6 and Chang8 member is mixed provenance,including west-south,west and east-north. By the study of rock types、 sedimentary conformation、lithology and electromotive curve combination and palaeo-biology,lake、delta and braided delta mianly developed in study area are recognized, Subaqueous distributary channels in delta front and in braided delta front, and sand body in deep-lake turbidite, are the main reservoir.forthermore,the characteristic of depositional system and sandy body in space are discussed. Applied with routine microscope slice identification, Scanning Electron Microscope, reservoir lithology and physical property analysis and other analytic machinery, Feldspar-lithic fine-sandstone and feldspar fine-sandstone are mainly sandstone of Y Chang6-Chang8 in Huanxian area, small pore and tiny pore are the main pore types, tiny throat type and micro-fine throat type are widely developed , secondary dissolution porosity, intercrystal porosity, tiny pore and micro-crack are main pore types.Intergranular porosity and dissolution porosity secondary is the main pore secondary. The dominant diagenesis types in the area are compaction, cementation, replacement and dissolution. Chlorite films cementation facies, carbonate cementation facies ,mud cementation compaction facie, compaction 、pressure solution facies are the main diagenetic facies,in which Chlorite films cementation facies is the best diagenetic facies in study area. Reservoir influence factor analysis ,rock types are the main factor forming this low-pore and low-permeability of Chang6-Chang8 member in study area,and relatively higher permeability area are cortrolled by sedimentary facies distribution, diagenesis improved reservoir physical property. According to the distributing of sedimentary micro-facies and sandy body , and the test oil, favorable region in Chang6-Chang8 are forecasted.

陕北何家集区延长组长4＋5－长6储层特征研究

Abstract： Hejiaji area lies on eastern part of Shanbei Slope in Ordos Basin and the primary oil-bearing bed is Chang 4＋5 and Chang 6 of Yanchang Formation. It is indicated that the sedimentary facies and reservoir characteristics restricted the hydrocarbon accumulation regularity by the geological information. Therefore, Applied with outcrop observation，core description, geophysical logging interpretation, thin section determination, Scanning Electron Microscope, reservoir lithology and physical property analysis and other analytic machinery, the sedimentary facies ,micro-characteristic and master control factors on hydrocarbon reservoir of Yanchang Formation in Hejiaji area are studied deeply by means of sedimentology，reservoir geology and petroleum geology and provide a reliably reference for later prospect . Delta facies are identified in Hejiaji area and of which distributary channels in delta plain microfacies controlled the distribution of sand bodies and accumulation of oil and gas.The distribution of sand bodies distributed from northeast to southwest are dominated by sedimentary facies . It was shown that the sandstones are medium to granule arkose，which the mud matrix is r and including，calcite，the content of matrix is lower and that mostly are cements which are mainly quartz and feldspar overgrowths and chlorite films, in the second place are hydromica and ferrocalcite. All the sandstones have entered a period of late diagenetic stage in which the dominant diagenesis types in the area are compaction, cementation and dissolution. Remnant intergranular porosity and feldspar dissolved pore are main pore types which are megalospore and medium pore. Medium-fine throat, fine throat and micro-fine throat are the mainly throat type. Pore texture can be classified as megalospore and fine throat type, medium-pore and micro-fine throat type mainly, and they are main accumulate interspace in research region. The reservoir of Yanchang Formation in Hejiaji area is low- pore and low- permeability in the mass which have strong heterogeneity in bed, interbedded and plane. Studying the parameter of pore and permeability comprehensively and consulting prevenient study results of evaluation of reservoir, the reservoir is classifiedⅡ，Ⅲ and Ⅳ three types in which the Ⅱand Ⅲ can be divided into Ⅱa and Ⅱb, Ⅲa and Ⅲb respectively. Ⅱb and Ⅲa are the main reservoir type in Hejiaji area which are about 72.73％and 80％percent of whole reservoir and effective reservoir respectively.

两种超微细矿物粉体材料的制备及应用研究

Superfine mineral materials are mainly resulted from the pulverization of natural mineral resources, and are a type of new materials that can replace traditional materials and enjoy the most extensive application and the highest degree of consumption in the present day market. As a result, superfine mineral materials have a very broad and promising prospect in terms of market potential. Superfine pulverization technology is the only way for the in-depth processing of most of the traditional materials, and is also one of the major means for which mineral materials can realize their application. China is rich in natural resources such as heavy calcite, kaolin, wollastonite, etc., which enjoy a very wide market of application in paper making, rubber, plastics, painting, coating, medicine, environment-friendly recycle paper and fine chemical industries, for example. However, because the processing of these resources is generally at the low level, economic benefit and scale for the processing of these resources have not been realized to their full potential even up to now. Big difference in product indices and superfine processing equipment and technologies between China and advanced western countries still exists. Based on resource assessment and market potential analysis, an in-depth study was carried out in this paper about the superfine pulverization technology and superfine pulverized mineral materials from the point of mineralogical features, determination of processing technologies, analytical methods and applications, by utilizing a variety of modern analytical methods in mineralogy, superfine pulverization technology, macromolecular chemistry, material science and physical chemistry together with computer technology and so on. The focus was placed on the innovative study about the in-depth processing technology and the processing apparatus for kaolin and heavy calcite as well as the application of superfine products. The main contents and the major achievements of this study are listed as follows: 1. Superfine pulverization processing of mineral materials shall be integrated with the study of their crystal structures and chemical composition. And special attention shall be put on the post-processing technologies, rather than on the indices for particle size, of these materials, based on their fields of application. Both technical feasibility and economic feasibility shall be taken into account for the study about superfine pulverization technologies, since these two kinds of feasibilities serve as the premise for the industrialized application of superfine pulverized mineral materials. Based on this principle, preposed chemical treatment method, technology of synchronized superfine pulverization and gradation, processing technology and apparatus of integrated modification and depolymerization were utilized in this study, and narrow distribution in terms of particle size, good dispersibility, good application effects, low consumption as well as high effectiveness of superfine products were achieved in this study. Heavy calcite and kaolin are two kinds of superfine mineral materials that enjoy the highest consumption in the industry. Heavy calcite is mainly applied in paper making, coating and plastics industries, the hard kaolin in northern China is mainly used in macromolecular materials and chemical industries, while the soft kaolin in southern China is mainly used for paper making. On the other hand, superfine pulverized heavy calcite and kaolin can both be used as the functional additives to cement, a kind of material that enjoys the biggest consumption in the world. A variety of analytical methods and instruments such as transmission and scanning electron microscopy, X-ray diffraction analysis, infrared analysis, laser particle size analysis and so on were applied for the elucidation of the properties and the mechanisms for the functions of superfine mineral materials as used in plastics and high-performance cement. Detection of superfine mineral materials is closely related to the post-processing and application of these materials. Traditional detection and analytical methods for superfine mineral materials include optical microscopy, infrared spectral analysis and a series of microbeam techniques such as transmission and scanning electron microscopy, X-ray diffraction analysis, and so on. In addition to these traditional methods, super-weak luminescent photon detection technology of high precision, high sensitivity and high signal to noise ratio was also utilized by the author for the first time in the study of superfine mineral materials, in an attempt to explore a completely new method and means for the study of the characterization of superfine materials. The experimental results are really exciting! The innovation of this study is represented in the following aspects: 1. In this study, preposed chemical treatment method, technology of synchronized superfine pulverization and gradation, processing technology and apparatus of integrated modification and depolymerization were utilized in an innovative way, and narrow distribution in terms of particle size, good dispersibility, good application effects, low consumption as well as high effectiveness of superfine products were achieved in the industrialized production process*. Moreover, a new modification technology and related directions for producing the chemicals were invented, and the modification technology was even awarded a patent. 2. The detection technology of super-weak luminescent photon of high precision, high sensitivity and high signal to noise ratio was utilized for the first time in this study to explore the superfine mineral materials, and the experimental results can be compared with those acquired with scanning electron microscopy and has demonstrated its unique advantages. It can be expected that further study may possibly help to result in a completely new method and means for the characterization of superfine materials. 3. During the heating of kaolinite and its decomposition into pianlinite, the diffraction peaks disappear gradually. First comes the disappearance of the reflection of the basal plane (001), and then comes the slow disappearance of the (hkl) diffraction peaks. And this was first discovered during the experiments by the author, and it has never before reported by other scholars. 4. The first discovery of the functions that superfine mineral materials can be used as dispersants in plastics, and the first discovery of the comprehensive functions that superfine mineral materials can also be used as activators, water-reducing agents and aggregates in high-performance cement were made in this study, together with a detailed discussion. This study was jointly supported by two key grants from Guangdong Province for Scientific and Technological Research in the 10th Five-year Plan Period (1,200,000 yuan for Preparation technology, apparatus and post-processing research by using sub-micron superfine pulverization machinery method, and 300,000 yuan for Method and instruments for biological photon technology in the characterization of nanometer materials), and two grants from Guangdong Province for 100 projects for scientific and technological innovation (700,000 yuan for Pilot experimentation of superfine and modified heavy calcite used in paper-making, rubber and plastics industry, and 400,000 yuan for Study of superfine, modified wollastonite of large length-to-diameter ratio).

A Multiple-Context Equality-Based Reasoning System

Expert systems are too slow. This work attacks that problem by speeding up a useful system component that remembers facts and tracks down simple consequences. The redesigned component can assimilate new facts more quickly because it uses a compact, grammar-based internal representation to deal with whole classes of equivalent expressions at once. It can support faster hypothetical reasoning because it remembers the consequences of several assumption sets at once. The new design is targeted for situations in which many of the stored facts are equalities. The deductive machinery considered here supplements stored premises with simple new conclusions. The stored premises include permanently asserted facts and temporarily adopted assumptions. The new conclusions are derived by substituting equals for equals and using the properties of the logical connectives AND, Or, and NOT. The deductive system provides supporting premises for its derived conclusions. Reasoning that involves quantifiers is beyond the scope of its limited and automatic operation. The expert system of which the reasoning system is a component is expected to be responsible for overall control of reasoning.

Diagnóstico Molecular na era da sequenciação de 3ª geração e da PCR digital

Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas

Vírus e retrovírus

Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas