998 resultados para Genoma funcional
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The SPECT (Single Photon Emission Computed Tomography) systems are part of a medical image acquisition technology which has been outstanding, because the resultant images are functional images complementary to those that give anatomic information, such as X-Ray CT, presenting a high diagnostic value. These equipments acquire, in a non-invasive way, images from the interior of the human body through tomographic mapping of radioactive material administered to the patient. The SPECT systems are based on the Gamma Camera detection system, and one of them being set on a rotational gantry is enough to obtain the necessary data for a tomographic image. The images obtained from the SPECT system consist in a group of flat images that describe the radioactive distribution on the patient. The trans-axial cuts are obtained from the tomographic reconstruction techniques. There are analytic and iterative methods to obtain the tomographic reconstruction. The analytic methods are based on the Fourier Cut Theorem (FCT), while the iterative methods search for numeric solutions to solve the equations from the projections. Within the analytic methods, the filtered backprojection (FBP) method maybe is the simplest of all the tomographic reconstruction techniques. This paper's goal is to present the operation of the SPECT system, the Gamma Camera detection system, some tomographic reconstruction techniques and the requisites for the implementation of this system in a Nuclear Medicine service
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Introdução – Uma das causas mais prováveis de lesões esportivas está relacionada aos desequilíbrios musculares e a fadiga muscular, que é evidenciada no decorrer de um jogo de futebol, principalmente no final do jogo. Esta pode ser avaliada através da comparação da força de contração máxima antes e após o exercício. A fadiga produzida durante o jogo diminui a força muscular concêntrica e excêntrica de flexores e extensores do joelho podendo aumentar a incidência de lesões. Objetivo - Avaliar o efeito de uma partida sobre o pico de torque funcional e convencional nos músculos isquiotibiais e quadríceps de jogadores de futebol de salão com e sem desequilíbrio muscular. Método – Oito jogadores de futebol de salão do gênero masculino, com idade entre 18 e 24 anos, fizeram três visitas ao laboratório: 1ª foram realizadas a antropometria e adaptação aos equipamentos; 2ª teste máximo no dinamômetro isocinético para determinar a razão do pico de torque funcional e convencional nos músculos isquiotibiais e quadríceps; 3ª idem ao 2ª, após a partida de futebol de salão. Análise estatística – através das médias ± DP dos dados obtidos foi utilizado o teste t para amostras dependentes e o Mann Whitney para comparação do pico de torque antes e após a partida de futebol de salão e para comparação da relação flexor/extensor pré e pós- esforço, respectivamente. Em ambos os testes foram adotado um nível de significância de p < 0,05. Resultados - Não foram encontradas diferenças significantes entre os grupos de atletas com desequilíbrio muscular na perna dominante (GD) e grupo equilíbrio (GE) e para os extensores e flexores do GD e GE nas condições Con60. Através do Mann Whitney foram encontradas diferenças...(Resumo completo, clicar acesso eletrônico abaixo)
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Mood States are feelings auto regulation deliver to individual better quality of life and healthy aging. The declines of the aging process can be minimized with the regular practice of physical activity and systematized. The objective of this study was to evaluate the effects of a dance program in mood states, depressive symptoms and functional capacity in elderly participants of PROFIT. METHOD: The sample was composed of 20 elderly people. The Training Group was formed by 15 elders who participated in a protocol of dance with duration of 1 hour, three times per week, for 12 weeks and the Control Group, composed of 5 elderly, did not attend any program of physical activity. The participants were evaluated by the List of States of Mind Reduced and Illustrated, Geriatric Depression Scale and the battery of tests of AAHPERD. For the mood states was used non-parametric analysis, according to the technique of Binomial Analysis. To compare the components of functional capacity between the groups was used analysis of variance for repeated measures two-way ANOVA and the level of significance was set at 5 %. RESULTS: The functional components remained favorable, highlighting Agility (pre = 21.03 ± 1.64 sec./ post = 18.63 ± 2.26 sec.) And aerobic endurance (pre = 495.94 ± 46.48 sec. / post = 448.01 ± 12.27 sec.). In the comparison between positive and negative functional levels and mood states showed no relationship between these variables, dealing with different aspects and has influence one another. The state post-dancing sessions mood in GT were more positive even for elderly patients with depressive symptoms even unproven statistically, depressive symptoms were maintained or reduced mostly. CONCLUSION: The dance practice maintains the functionality of components; modifies mood states to positive levels and reduces or prevents the onset of depressive symptoms and complications in elderly
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The Coffee Genome Project made available to the scientific community relevant information that made practical the identification and cloning of important genes, as well as the identification of the major sequences involved on their regulation. The aim of the present study was to amplify, clone and sequence coffee promoters with specific expression patterns. For that, coffee ESTs which known expression profiles were employed. First, the promoter regions of coffee genes showing, respectively, fruitspecific and ubiquitous expression were amplified using the Genome Walking strategy. Amplified sequences were then inserted in the pGEM-Teasy vector (Promega) and sequenced. Once completed the sequencing, an expression cassette was constructed using the binary vector pCAMBIA-1381z (Cambia). These expression cassettes were cloned into Agrobacterium tumefaciens, and transgenic tobacco plants were generated aiming the functional characterization of these promoters
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Introdução: O envelhecimento e/ou fatores a ele associados pode levar à diminuição dos componentes de capacidade funcional (CF). Tal redução pode comprometer a realização das atividades da vida diária (AVDs), a independência e a qualidade de vida do idoso. A avaliação de tais componentes reveste-se de importância para conhecer o estado funcional bem como prescrever programas de atividades físicas para a população idosa. Objetivo: Analisar os níveis dos componentes de CF em idosos com 80 anos ou mais de idade, assim como verificar e comparar os resultados entre gêneros desse mesmo estudo e também com outras faixas de idade através de resultados já existentes na literatura. Método: Sessenta idosos (30 homens e 30 mulheres) com idade de 80 anos ou mais realizaram a bateria de testes motores da American Alliance for Health, Physical Education, Recreation and Dance (AAHPERD), que avaliou os seis componentes de CF (flexibilidade, coordenação, agilidade, equilíbrio, força e capacidade aeróbia). Os dados foram analisados pela estatística descritiva e pela comparação de médias entre os dois gêneros e com os resultados, já previamente existentes, de outras faixas etárias, por meio do teste t de Student e teste t (resumo amostral) para p <0,05. Resultados: A comparação entre gêneros apresentou resultados com diferença estatisticamente significativa no componente capacidade aeróbia, com melhores valores para os homens, e nos componentes força e flexibilidade com melhores resultados para as mulheres. A comparação com faixas etárias mais jovens revelou diferença estatisticamente significativa para todos os componentes, sendo que apenas a flexibilidade apresentou melhores resultados para as idosas com 80 anos ou mais. Conclusão: Conclui-se, dessa forma, que homens com 80 anos ou mais de idade possuem menores valores de flexibilidade e resistência de força... (Resumo completo, clicar acesso eletrônico abaixo)
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In the present work we performed molecular cytogenetic studies in two different populations of Astyanax bockmanni from streams of Botucatu, SP: one from Capivara river, on Tietê river basin and one from Água da Madalena river, on Paranapanema river basin. The results showed that the population of Astyanax bockmanni from Capivara river have a diploid number of 50 chromosomes, with karyotype consisting of 8 methacentric, 14 submethacentric, 12 subtelocentric and 16 acrocentric, while the individuals of the population from Água da Madalena river have 50 diploid chromosomes but with karyotype organized in 8 metacentric, 14 submetacentric, 16 subtelocentric and 12 acrocentric. Also, the rDNA 18s sequences are widely dispersed throughout the genome of two populations, with intra and interindividual variations. On the other hand, the sequences for rDNA 5S and Histone H1 remained chromosomally conserved in these two samples and sites located in pairs 2 and 19 (rDNA 5S) and the pairs 2 and 15 (Histone H1). The low dispersion of structural genes and a functional dynamic independence between sequences of rDNA 5S and rDNA 18S may be related to the process of karyotype maintenance and differentiation in these populations of Astyanax bockmanni
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Currently the forests around the world face different aspects of weather. Global climate change and its adversities alter significantly the dynamics of the forest, directly influencing the operation. Human activities interfere in the functions of forests, either by removal of vegetation, either by reforesting deforested areas. Given the influence of human activity and climate change on forests, this project presents a comparative and analytical situation, the microclimatic level, the State Forest Edmundo Navarro de Andrade (FEENA), in Rio Claro. We measured the temperature maximum, minimum and average in different periods surrounding the FEENA, from 1889 to 1917, 1971 to 1999 and from 1999 to 2011. What allowed to suggest changes in the microclimate of your surroundings and probably in forest dynamics
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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV
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Pós-graduação em Microbiologia Agropecuária - FCAV
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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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This work deals with the distinction between embedded questions and headless relatives, that was already pointed out as problematic in several studies based on generative approach. The purpose is to examine to what extent functional aspects, as proposed by the theory of Functional Discourse Grammar (HENGEVELD; MACKENZIE, 2008) to describe the two types of clauses can contribute to the explanation of the differences and similarities between them. Based on representative occurrences of spoken Brazilian Portuguese, we demonstrated that the approach of Functional Discourse Grammar provides important parameters for defining the nature of these two types of clauses, in both formal and functional (semantic-discursive) terms, contributing thus to the existing proposals for the distinction between these clauses.
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Conselho Nacional de Desenvolvimento em Pesquisa (CNPq)
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Conselho Nacional de Desenvolvimento em Pesquisa (CNPq)