DNA recognition by the first tail-to-tail linked distamycin-like oligopeptide dimers

Sequence-specific bidentate binding to double-stranded (ds)-DNA by 'tail-to-tail' linked dimeric, distamycin analogues is described; compared to their monomeric analogues, these dimers exhibit greater affinity and longer binding site size and open up a novel avenue in the design of minor groove binders that overcome the phasing problem.

Conformational basis for substrate recognition and regulation of catalytic activity in Staphylococcus aureus nucleoside di-phosphate kinase

Nucleoside diphosphate kinases (NDK) are characterized by high catalytic turnover rates and diverse substrate specificity. These features make this enzyme an effective activator of a pro-drug an application that has been actively pursued for a variety of therapeutic strategies. The catalytic mechanism of this enzyme is governed by a conserved histidine that coordinates a magnesium ion at the active site. Despite substantial structural and biochemical information on NDK, the mechanistic feature of the phospho-transfer that leads to auto-phosphorylation remains unclear. While the role of the histidine residue is well documented, the other active site residues, in particular the conserved serine remains poorly characterized. Studies on some homologues suggest no role for the serine residue at the active site, while others suggest a crucial role for this serine in the regulation and quaternary association of this enzyme in some species. Here we report the biochemical features of the Staphylococcus aureus NDK and the mutant enzymes. We also describe the crystal structures of the apo-NDK, as a transition state mimic with vanadate and in complex with different nucleotide substrates. These structures formed the basis for molecular dynamics simulations to understand the broad substrate specificity of this enzyme and the role of active site residues in the phospho-transfer mechanism and oligomerization. Put together, these data suggest that concerted changes in the conformation of specific residues facilitate the stabilization of nucleotide complexes thereby enabling the steps involved in the ping-pong reaction mechanism without large changes to the overall structure of this enzyme. (C) 2011 Elsevier B.V. All rights reserved.

Two Dimensional Principal Component Analysis for Online Tamil Character Recognition

This paper presents a new application of two dimensional Principal Component Analysis (2DPCA) to the problem of online character recognition in Tamil Script. A novel set of features employing polynomial fits and quartiles in combination with conventional features are derived for each sample point of the Tamil character obtained after smoothing and resampling. These are stacked to form a matrix, using which a covariance matrix is constructed. A subset of the eigenvectors of the covariance matrix is employed to get the features in the reduced sub space. Each character is modeled as a separate subspace and a modified form of the Mahalanobis distance is derived to classify a given test character. Results indicate that the recognition accuracy using the 2DPCA scheme shows an approximate 3% improvement over the conventional PCA technique.

Online character recognition using regression techniques

This paper introduces a scheme for classification of online handwritten characters based on polynomial regression of the sampled points of the sub-strokes in a character. The segmentation is done based on the velocity profile of the written character and this requires a smoothening of the velocity profile. We propose a novel scheme for smoothening the velocity profile curve and identification of the critical points to segment the character. We also porpose another method for segmentation based on the human eye perception. We then extract two sets of features for recognition of handwritten characters. Each sub-stroke is a simple curve, a part of the character, and is represented by the distance measure of each point from the first point. This forms the first set of feature vector for each character. The second feature vector are the coeficients obtained from the B-splines fitted to the control knots obtained from the segmentation algorithm. The feature vector is fed to the SVM classifier and it indicates an efficiency of 68% using the polynomial regression technique and 74% using the spline fitting method.

Comparison of AM-FM Based Features For Robust Speech Recognition

Effective feature extraction for robust speech recognition is a widely addressed topic and currently there is much effort to invoke non-stationary signal models instead of quasi-stationary signal models leading to standard features such as LPC or MFCC. Joint amplitude modulation and frequency modulation (AM-FM) is a classical non-parametric approach to non-stationary signal modeling and recently new feature sets for automatic speech recognition (ASR) have been derived based on a multi-band AM-FM representation of the signal. We consider several of these representations and compare their performances for robust speech recognition in noise, using the AURORA-2 database. We show that FEPSTRUM representation proposed is more effective than others. We also propose an improvement to FEPSTRUM based on the Teager energy operator (TEO) and show that it can selectively outperform even FEPSTRUM

Online Handwriting Recognition of Tamil Script Using Fractal Geometry

We present a fractal coding method to recognize online handwritten Tamil characters and propose a novel technique to increase the efficiency in terms of time while coding and decoding. This technique exploits the redundancy in data, thereby achieving better compression and usage of lesser memory. It also reduces the encoding time and causes little distortion during reconstruction. Experiments have been conducted to use these fractal codes to classify the online handwritten Tamil characters from the IWFHR 2006 competition dataset. In one approach, we use fractal coding and decoding process. A recognition accuracy of 90% has been achieved by using DTW for distortion evaluation during classification and encoding processes as compared to 78% using nearest neighbor classifier. In other experiments, we use the fractal code, fractal dimensions and features derived from fractal codes as features in separate classifiers. While the fractal code is successful as a feature, the other two features are not able to capture the wide within-class variations.

Unrestricted Kannada online handwritten akshara recognition using SDTW

In this paper, we present an unrestricted Kannada online handwritten character recognizer which is viable for real time applications. It handles Kannada and Indo-Arabic numerals, punctuation marks and special symbols like $, &, # etc, apart from all the aksharas of the Kannada script. The dataset used has handwriting of 69 people from four different locations, making the recognition writer independent. It was found that for the DTW classifier, using smoothed first derivatives as features, enhanced the performance to 89% as compared to preprocessed co-ordinates which gave 85%, but was too inefficient in terms of time. To overcome this, we used Statistical Dynamic Time Warping (SDTW) and achieved 46 times faster classification with comparable accuracy i.e. 88%, making it fast enough for practical applications. The accuracies reported are raw symbol recognition results from the classifier. Thus, there is good scope of improvement in actual applications. Where domain constraints such as fixed vocabulary, language models and post processing can be employed. A working demo is also available on tablet PC for recognition of Kannada words.

Comparison of HMM and SDTW for Tamil handwritten character recognition

In this paper, we compare the experimental results for Tamil online handwritten character recognition using HMM and Statistical Dynamic Time Warping (SDTW) as classifiers. HMM was used for a 156-class problem. Different feature sets and values for the HMM states & mixtures were tried and the best combination was found to be 16 states & 14 mixtures, giving an accuracy of 85%. The features used in this combination were retained and a SDTW model with 20 states and single Gaussian was used as classifier. Also, the symbol set was increased to include numerals, punctuation marks and special symbols like $, & and #, taking the number of classes to 188. It was found that, with a small addition to the feature set, this simple SDTW classifier performed on par with the more complicated HMM model, giving an accuracy of 84%. Mixture density estimation computations was reduced by 11 times. The recognition is writer independent, as the dataset used is quite large, with a variety of handwriting styles.

Self assembly of bivalent glycolipids on single walled carbon nanotubes and their specific molecular recognition properties

Solubilization of single walled carbon nanotubes (SWNTs) in aqueous milieu by self assembly of bivalent glycolipids is described. Thorough analysis of the resulting composites involving Vis/near-IR spectroscopy, surface plasmon resonance, confocal Raman and atomic force microscopy reveals that glycolipid-coated SWNTs possess specific molecular recognition properties towards lectins.

Prototype learning methods for online handwriting recognition

In this paper, we study different methods for prototype selection for recognizing handwritten characters of Tamil script. In the first method, cumulative pairwise- distances of the training samples of a given class are used to select prototypes. In the second method, cumulative distance to allographs of different orientation is used as a criterion to decide if the sample is representative of the group. The latter method is presumed to offset the possible orientation effect. This method still uses fixed number of prototypes for each of the classes. Finally, a prototype set growing algorithm is proposed, with a view to better model the differences in complexity of different character classes. The proposed algorithms are tested and compared for both writer independent and writer adaptation scenarios.

A Long Memory Process Based Parametric Modeling and Recognition of PD Signal

We address the problem of recognition and retrieval of relatively weak industrial signal such as Partial Discharges (PD) buried in excessive noise. The major bottleneck being the recognition and suppression of stochastic pulsive interference (PI) which has similar time-frequency characteristics as PD pulse. Therefore conventional frequency based DSP techniques are not useful in retrieving PD pulses. We employ statistical signal modeling based on combination of long-memory process and probabilistic principal component analysis (PPCA). An parametric analysis of the signal is exercised for extracting the features of desired pules. We incorporate a wavelet based bootstrap method for obtaining the noise training vectors from observed data. The procedure adopted in this work is completely different from the research work reported in the literature, which is generally based on deserved signal frequency and noise frequency.