Alternative drying techniques for the manufacturing of hydrogel-forming and dissolving microneedle arrays.

Dissolving polymeric microneedle arrays and hydrogel-forming microneedle arrays have attracted much attention during recent years due mainly to their biocompatibility and capacity for enhanced drug delivery. Nevertheless, for the production of this type of devices, typically, a drying step is required. Microneedles are prepared following a micromoulding technique using aqueous blends of Gantrez® S-97. Currently, production of microneedles arrays involves a long drying process of 48 hours. Therefore alternative drying methods were investigated including microwave radiation and hot air convection.

The Use of a Pressure-Indicating Sensor Film to Provide Feedback upon Hydrogel-Forming Microneedle Array Self-Application In Vivo

PURPOSE:
To evaluate the combination of a pressure-indicating sensor film with hydrogel-forming microneedle arrays, as a method of feedback to confirm MN insertion in vivo.
METHODS:
Pilot in vitro insertion studies were conducted using a Texture Analyser to insert MN arrays, coupled with a pressure-indicating sensor film, at varying forces into excised neonatal porcine skin. In vivo studies involved twenty human volunteers, who self-applied two hydrogel-forming MN arrays, one with a pressure-indicating sensor film incorporated and one without. Optical coherence tomography was employed to measure the resulting penetration depth and colorimetric analysis to investigate the associated colour change of the pressure-indicating sensor film.
RESULTS:
Microneedle insertion was achieved in vitro at three different forces, demonstrating the colour change of the pressure-indicating sensor film upon application of increasing pressure. When self-applied in vivo, there was no significant difference in the microneedle penetration depth resulting from each type of array, with a mean depth of 237 μm recorded. When the pressure-indicating sensor film was present, a colour change occurred upon each application, providing evidence of insertion.
CONCLUSIONS:
For the first time, this study shows how the incorporation of a simple, low-cost pressure-indicating sensor film can indicate microneedle insertion in vitro and in vivo, providing visual feedback to assure the user of correct application. Such a strategy may enhance usability of a microneedle device and, hence, assist in the future translation of the technology to widespread clinical use.

Hur ett tvärfunktionellt samarbete kan motverka stuprörsstrukturer : Kommunikationens roll i det organisatoriska gränssnittet mellan olika avdelningar

Stovepipes, or also called silos, appear in many different organizations and sectors and contribute to problems when employees or managers tend to look more to their own, or the individual departments, objectives rather than to the organizations. The purpose of this study was to identify different communicative factors that promote stovepipes in order to further identify the most critical factor to disarm. A case study has been done at a selected company, with a stovepipe structure, in order to achieve the purpose of the study. The case study has included interviews and observations to identify different problem areas which then have been compared with three communicative factors identified in previous studies. The factor that had the most connections to the problem areas have been considered the most critical factor. The result of the study indicates that “A lack of understanding each other's work” is the most critical factor in stovepipe structures and that it can be prevented by following five recommendations: bring up positive collaboration continually, raise problems with each other instead of with others, identify different communication paths in and between the departments, implement a long-term model for preventing stovepipes and set up workshops between the involved departments. The conclusion of the study is that stovepipes create several undesirable effects in the organization but that the efforts to counter these problems do not have to be complicated. Following five small steps into a better collaboration and communication can be enough to be on your way to a better organizational structure.

Catalytic reactions with palladium supported on mesocellular foam : Applications in hydrogenation, isomerization, and C-C bond forming reactions

The major part of this thesis concerns the development of catalytic methodologies based on palladium nanoparticles immobilized on aminopropyl-functionalized siliceous mesocellular foam (Pd0-AmP-MCF). The catalytic activity of the precursor to the nanocatalyst, PdII-AmP-MCF is also covered by this work. In the first part the application of Pd0-AmP-MCF in Suzuki-Miyaura cross-coupling reactions and transfer hydrogenation of alkenes under microwave irradiation is described. Excellent reactivity was observed and a broad range of substrates were tolerated for both transformations. The Pd0-AmP-MCF exhibited high recyclability as well as low metal leaching in both cases. The aim of the second part was to evaluate the catalytic efficiency of the closely related PdII-AmP-MCF for cycloisomerization of various acetylenic acids. The catalyst was able to promote formation of lactones under mild conditions using catalyst loadings of 0.3 - 0.5 mol% at temperatures of up to 50 oC in the presence of Et3N. By adding 1,4-benzoquinone to the reaction, the catalyst could be recycled four times without any observable decrease in the activity. The selective arylation of indoles at the C-2 position using Pd-AmP-MCF and symmetric diaryliodonium salts is presented in the third part. These studies revealed that Pd0-AmP-MCF was more effective than PdII-AmP-MCF for this transformation. Variously substituted indoles as well as diaryliodonium salts were tolerated, giving arylated indoles in high yields within 15 h at 20 - 50 oC in H2O. Only very small amounts of Pd leaching were observed and in this case the catalyst exhibited moderate recyclability. The final part of the thesis describes the selective hydrogenation of the C=C in different α,β-unsaturated systems. The double bond was efficiently hydrogenated in high yields both under batch and continuous-flow conditions. High recyclability and low metal leaching were observed in both cases.

”Få in världen i klassrummet” : En studie om skönlitteraturens roll i gymnasieskolans SVAundervisning

Uppsatsens övergripande syfte är att åskådliggöra hur några gymnasielärare behöriga i svenska som andraspråk respektive svenska betraktar skönlitteraturens roll i ämnet svenska som andraspråk (SVA) och hur det praktiska klassrumsarbetet med litteraturundervisning ser ut. I uppsatsen diskuteras även lärarnas syn på skönlitteraturen i SVA-ämnet i jämförelse med i svenskämnet i relation ämnes- och kursplanerna i Läroplan, examensmål och gymnasiegemensamma ämnen för gymnasieskola 2011. För att uppnå syftet och besvara frågeställningarna har jag genomfört en kvalitativ studie där fyra lärare intervjuats, samtliga verksamma på nationella program. Av resultaten framkommer att lärarna starkt betonar skönlitteraturens betydelse i SVA-undervisningen. De viktigaste motiven för skönlitteraturen som lyfts fram är språkutveckling, identitetsutveckling, främjande av empatisk och demokratisk förmåga samt som förmedlare av svenska referensramar. Vid valen av litterära texter beaktar lärarna styrdokumentens skrivningar samt språkliga faktorer. Två arbetssätt som framhålls är boksamtal och tematiskt arbete. Det litterära samtalet framhävs som positivt för lärandet liksom läsande i en större kontext inom ett gemensamt tema. Lärarna understryker att skillnaderna i styrdokumenten mellan svenskämnena är alltför stora vad avser skrivningar om skönlitteratur och de anser att SVA borde likna svenskämnet mera vad gäller det litterära ämnesinnehållet.

Begreppens roll vid inlärning av algebra : En litteraturstudie om den roll begrepp och begreppsförmåga spelar vid inlärning av algebra för elever i årskurs 4-6

Svenska elever har presterat dåligt i internationella undersökningar en längre tid när det gäller algebraområdet i matematik. Elevernas begreppsförståelse har pekats ut som en faktor som spelar in i de dåliga resultaten för svenska elever del. Syftet med denna studie har därför varit att ta reda på den roll som begrepp och begreppsförmåga spelar vid inlärning av algebra samt vilken begreppsförståelse elever i årskurs 4-6 har. Genom en systematisk litteraturstudie har frågeställningarna besvarats. Resultaten visar att brister i begreppsförståelse i algebra också leder till brister i kunskap i algebra. Undervisning med fokus på begrepp leder till bättre förståelse för begrepp samtidigt som det även leder till procedurell kunskap. Elever i årskurs 4-6 kan hantera variabler och använda dem i matematiska uttryck. Fördelar med en tidig introduktion av variabelbegreppet är att elever bygger en bättre förståelse för begreppet.

Begreppens roll vid inlärning av algebra : En litteraturstudie om den roll begrepp och begreppsförmåga spelar vid inlärning av algebra för elever i årskurs 4-6

Svenska elever har presterat dåligt i internationella undersökningar en längre tid när det gäller algebraområdet i matematik. Elevernas begreppsförståelse har pekats ut som en faktor som spelar in i de dåliga resultaten för svenska elever del. Syftet med denna studie har därför varit att ta reda på den roll som begrepp och begreppsförmåga spelar vid inlärning av algebra samt vilken begreppsförståelse elever i årskurs 4-6 har. Genom en systematisk litteraturstudie har frågeställningarna besvarats. Resultaten visar att brister i begreppsförståelse i algebra också leder till brister i kunskap i algebra. Undervisning med fokus på begrepp leder till bättre förståelse för begrepp samtidigt som det även leder till procedurell kunskap. Elever i årskurs 4-6 kan hantera variabler och använda dem i matematiska uttryck. Fördelar med en tidig introduktion av variabelbegreppet är att elever bygger en bättre förståelse för begreppet.

Large-scale Roll Out of Online Prospective Measurement of Mood Symptoms in Affective Disorders

Background and Aims: True Colours is an online prospective mood-monitoring system developed at the University of Oxford to assist local patients and clinicians with monitoring course of illness in bipolar disorder. We report our initial experiences of using True Colours for research purposes in the Bipolar Disorder Research Network (BDRN; www.bdrn.org), a large research network of individuals with mood disorders spread throughout the UK. Methods: After initial piloting to ensure the practicality/acceptability of using True Colours within BDRN, we invited all BDRN participants (n = 7000) to participate in weekly True Colours ratings via three postal invitations sent over an 8-month period. Results: Following the three postal invitations, 915 individuals have so far expressed an interest in joining True Colours, and, of these, 662 (72.3%) have registered. 32 of those who registered for True Colours (5%) have so far asked to leave the system. Positive feedback from participants has focused around the ease of use and convenience of True Colours and potential clinical utility of the graphical representation of weekly mood scores. Conclusions: We have demonstrated that large-scale prospective mood monitoring for research purposes using a contemporary online approach is feasible. Challenges have included: (i) variation in participants’ technological ability; (ii) management of requests for clinical advice based on mood scores within a research setting; and, (iii) resources required to provide access and on-going support for participants using True Colours. We continue to expand recruitment to True Colours within BDRN, and plan to trial email invitations in the next phase of recruitment.

Samhällskunskap och historia i svensk gymnasieskola: ämnenas roll och relation i diskurs och ämnesplaner

The adoption of new syllabuses in history and social science in the new Swedish curriculum raises questions on the relationship between the two subjects. Social Science and History as school subjects have previously been described as both competing and complementary. The competing aspect has mainly been descibed in terms of citizenship education and the complementary aspect has been described in terms of content, abilities, and temporality. This article discusses how history and social science relate to these four topics in didactic discourse and in the new syllabuses. In the didactic discource both subjects underline the importance of making subject matter useful in life outside school. However, the new syllabuses are very differently formulated in this sense. History is closely connected to citizenship education and describes content, abilities and temporality in such a context. Social Science on the other hand downtunes the traditional role as a subject important for citizenship. Instead, focus is aimed at disciplinary thinking and using knowledge to understand social science methods and theories. This development is seen as very problematic and challenging for a meaningful teaching of Social Science in upper secondary school. In response to this challenge, the author proposes interdisciplinary didactic development, especially in developing "dynamic concepts" within Social Science didactics.

Channel-forming activity of syringopeptin 25 A in mercury-supported phospholipid monolayers and negatively charged bilayers

Interactions of the cationic lipodepsipeptide syringopeptin 25 A (SP25A) with mercury-supported dioleoylphosphatidylcholine (DOPC), dioleoylphosphatidylserine (DOPS) and dioeleoylphosphatidic acid (DOPA) self-assembled monolayers (SAMs) were investigated by AC voltammetry in 0.1 M KCl at pH 3, 5.4 and 6.8. SP25A targets and penetrates the DOPS SAM much more effectively than the other SAMs not only at pH 6.8, where the DOPS SAM is negatively charged, but also at pH 3, where it is positively charged just as SP25A. Similar investigations at tethered bilayer lipid membranes (tBLMs) consisting of a thiolipid called DPTL anchored to mercury, with a DOPS, DOPA or DOPC distal monolayer on top of it, showed that, at physiological transmembrane potentials, SP25A forms ion channels spanning the tBLM only if DOPS is the distal monolayer. The distinguishing chemical feature of the DOPS SAM is the ionic interaction between the protonated amino group of a DOPS molecule and the carboxylate group of an adjacent phospholipid molecule. Under the reasonable assumption that SP25A preferentially interacts with this ion pair, the selective lipodepsipeptide antimicrobial activity against Gram-positive bacteria may be tentatively explained by its affinity for similar protonated amino-carboxylate pairs, which are expected to be present in the peptide moieties of peptidoglycan strands.

Ionisation and discharge in cloud-forming atmospheres of brown dwarfs and extrasolar planets

Brown dwarfs and giant gas extrasolar planets have cold atmospheres with rich chemical compositions from which mineral cloud particles form. Their properties, like particle sizes and material composition, vary with height, and the mineral cloud particles are charged due to triboelectric processes in such dynamic atmospheres. The dynamics of the atmospheric gas is driven by the irradiating host star and/or by the rotation of the objects that changes during its lifetime. Thermal gas ionisation in these ultra-cool but dense atmospheres allows electrostatic interactions and magnetic coupling of a substantial atmosphere volume. Combined with a strong magnetic field , a chromosphere and aurorae might form as suggested by radio and x-ray observations of brown dwarfs. Non-equilibrium processes like cosmic ray ionisation and discharge processes in clouds will increase the local pool of free electrons in the gas. Cosmic rays and lighting discharges also alter the composition of the local atmospheric gas such that tracer molecules might be identified. Cosmic rays affect the atmosphere through air showers in a certain volume which was modelled with a 3D Monte Carlo radiative transfer code to be able to visualise their spacial extent. Given a certain degree of thermal ionisation of the atmospheric gas, we suggest that electron attachment to charge mineral cloud particles is too inefficient to cause an electrostatic disruption of the cloud particles. Cloud particles will therefore not be destroyed by Coulomb explosion for the local temperature in the collisional dominated brown dwarf and giant gas planet atmospheres. However, the cloud particles are destroyed electrostatically in regions with strong gas ionisation. The potential size of such cloud holes would, however, be too small and might occur too far inside the cloud to mimic the effect of, e.g. magnetic field induced star spots.

Silicon Promotes Exodermal Casparian Band Formation in Si-Accumulating and Si-Excluding Species by Forming Phenol Complexes

We studied the effect of Silicon (Si) on Casparian band (CB) development, chemical composition of the exodermal CB and Si deposition across the root in the Si accumulators rice and maize and the Si non-accumulator onion. Plants were cultivated in nutrient solution with and without Si supply. The CB development was determined in stained root cross-sections. The outer part of the roots containing the exodermis was isolated after enzymatic treatment. The exodermal suberin was transesterified with MeOH/BF3 and the chemical composition was measured using gas chromatography-mass spectroscopy (GC-MS) and flame ionization detector (GC-FID). Laser ablation-inductively coupled plasma-mass spectroscopy (LA-ICP-MS) was used to determine the Si deposition across root cross sections. Si promoted CB formation in the roots of Si-accumulator and Si non-accumulator species. The exodermal suberin was decreased in rice and maize due to decreased amounts of aromatic suberin fractions. Si did not affect the concentration of lignin and lignin-like polymers in the outer part of rice, maize and onion roots. The highest Si depositions were found in the tissues containing CB. These data along with literature were used to suggest a mechanism how Si promotes the CB development by forming complexes with phenols.