Relatório de Estágio em Educação Pré-Escolar

O relatório de estágio de qualificação profissional desenvolveu-se no âmbito da Unidade Curricular (UC) de Prática Pedagógica Supervisionada, inserida no Mestrado em Educação Pré- Escolar, nos contextos de creche e pré-escolar. Esta integração implicou a mestranda numa atitude investigativa, traduzida no aprofundamento dos referenciais teóricos abordados na referida UC, que permitiu desenvolver uma atitude indagadora e reflexiva, de modo a aliar saberes teóricos à prática e mobilizá-los em contexto. Efetivando a sua prática pedagógica através da observação, recolha de dados, análise sistemática da ação e reflexão crítica permanente, apoiou-se na metodologia de investigaçãoação, que segundo Elliot (1991) constitui um processo reflexivo, que envolve a mudança de teorias e práticas, permitindo ligar a autoavaliação ao desenvolvimento profissional. A prática pedagógica revista neste documento parte da convicção da mestranda relativamente à importância da observação, na mesma perspetiva de Gonçalves (2006): “se soubermos observar, saberemos compreender, se soubermos compreender, saberemos intervir e se soubermos intervir, saberemos melhorar, estaremos a conferir ao exercício de observação e análise da relação educativa o seu verdadeiro estatuto de instrumento de desenvolvimento pessoal/profissional do formando” (p.72). Foi assim que a mestranda solidificou a construção da sua imagem de criança, encarando-a como ativa e competente, almejando que essa visão se refletisse nas suas planificações, que identificavam necessidades e interesses das crianças, e consequentemente na sua ação. A co construção de saberes e a cooperação assumiram particular relevância no processo de formação e desenvolvimento profissional, proporcionado pelos intervenientes no contexto educativo: par de formação, educadoras, supervisora, pais e comunidade.

Two protocols without periodicity for the global and preemptive scheduling problem of multi-mode real-time systems upon multiprocessor Platforms

We consider the problem of scheduling a multi-mode real-time system upon identical multiprocessor platforms. Since it is a multi-mode system, the system can change from one mode to another such that the current task set is replaced with a new task set. Ensuring that deadlines are met requires not only that a schedulability test is performed on tasks in each mode but also that (i) a protocol for transitioning from one mode to another is specified and (ii) a schedulability test for each transition is performed. We propose two protocols which ensure that all the expected requirements are met during every transition between every pair of operating modes of the system. Moreover, we prove the correctness of our proposed algorithms by extending the theory about the makespan determination problem.

Interactive light and sound table: an EPS@ISEP project

Interactive products are appealing objects in a technology-driven society and the offer in the market is wide and varied. Most of the existing interactive products only provide either light or sound experiences. Therefore, the goal of this project was to develop a product aimed for children combining both features. This project was developed by a team of four thirdyear students with different engineering backgrounds and nationalities during the European Project Semester at ISEP (EPS@ISEP) in 2012. This paper presents the process that led to the development of an interactive sound table that combines nine identical interaction blocks, a control block and a sound block. Each interaction block works independently and is composed of four light emitting diodes (LED) and one infrared (IR) sensor. The control is performed by an Arduino microcontroller and the sound block includes a music shield and a pair of loud speakers. A number of tests were carried out to assess whether the controller, IR sensors, LED, music shield and speakers work together properly and if the ensemble was a viable interactive light and sound device for children.

A fluorescente bicyclic Calix[4] Arene-Oxacyclophane with planar chirality: Resolution, chiroptical, properties, and absolute configuration.

A new inherently chiral calix[4]arene ICC 1 has been disclosed. The dissymmetry of 1 is generated from a chirality plane in the quinol moiety of a 1,3-bridged bicyclic calix[4]arene. ICC 1 has been resolved by enantioselective HPLC, and the chiroptical properties of both isolated antipodes (pS)-1 and (pR)-1 confirm their enantiomeric nature. The absolute configuration of the (pS)-1/(pR)-1 enantiomeric pair was established through time-dependent density functional theory (TDDFT) calculations of electronic circular dichroism (CD) spectra. (C) 2014 Elsevier Ltd. All rights reserved.

Estratégias para o desenvolvimento da competência escrita

Relatório de Estágio apresentado à Escola Superior de Educação de Lisboa para obtenção de grau de mestre em Ensino do 1.º e 2.º Ciclo

Neutral C60 effusive source for atomic collisions with fullerene

Review of scientific instruments, Vol.72, Nº9

Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.

Nucleic Acid Research (2007) Vol.37 N. 14 4755-4766

Tradições orais como subsídio para escritas de cena: das tradições orais à artes performativas

Projeto para obtenção do Grau de Mestre em Teatro. Especialização em Artes Performativas — Escritas de Cena.

Enantiomeric fraction evaluation of pharmaceuticals in environmentalmatrices by liquid chromatography-tandem mass spectrometry

The interest for environmental fate assessment of chiral pharmaceuticals is increasing and enantioselective analytical methods are mandatory. This study presents an enantioselective analytical method for the quantification of seven pairs of enantiomers of pharmaceuticals and a pair of a metabolite. The selected chiral pharmaceuticals belong to three different therapeutic classes, namely selective serotonin reuptake inhibitors (venlafaxine, fluoxetine and its metabolite norfluoxetine), beta-blockers (alprenolol, bisoprolol, metoprolol, propranolol) and a beta2-adrenergic agonist (salbutamol). The analytical method was based on solid phase extraction followed by liquid chromatography tandem mass spectrometry with a triple quadrupole analyser. Briefly, Oasis® MCX cartridges were used to preconcentrate 250 mL of water samples and the reconstituted extracts were analysed with a Chirobiotic™ V under reversed mode. The effluent of a laboratory-scale aerobic granular sludge sequencing batch reactor (AGS-SBR) was used to validate the method. Linearity (r2 > 0.99), selectivity and sensitivity were achieved in the range of 20–400 ng L−1 for all enantiomers, except for norfluoxetine enantiomers which range covered 30–400 ng L−1. The method detection limits were between 0.65 and 11.5 ng L−1 and the method quantification limits were between 1.98 and 19.7 ng L−1. The identity of all enantiomers was confirmed using two MS/MS transitions and its ion ratios, according to European Commission Decision 2002/657/EC. This method was successfully applied to evaluate effluents of wastewater treatment plants (WWTP) in Portugal. Venlafaxine and fluoxetine were quantified as non-racemic mixtures (enantiomeric fraction ≠ 0.5). The enantioselective validated method was able to monitor chiral pharmaceuticals in WWTP effluents and has potential to assess the enantioselective biodegradation in bioreactors. Further application in environmental matrices as surface and estuarine waters can be exploited.

Angular distributions in t(t)over-barH(H -> b(b)over-bar) reconstructed events at the LHC

The associated production of a Higgs boson and a top-quark pair, t (t) over barH, in proton-proton collisions is addressed in this paper for a center of mass energy of 13 TeV at the LHC. Dileptonic final states of t (t) over barH events with two oppositely charged leptons and four jets from the decays t -> bW(+) -> bl(+)v(l), (t) over bar -> (b) over barW(-) -> (b) over barl(-)(v) over bar (l) and h -> b (b) over bar are used. Signal events, generated with MadGraph5_aMC@NLO, are fully reconstructed by applying a kinematic fit. New angular distributions of the decay products as well as angular asymmetries are explored in order to improve discrimination of t (t) over barH signal events over the dominant irreducible background contribution, t (t) over barb (b) over bar. Even after the full kinematic fit reconstruction of the events, the proposed angular distributions and asymmetries are still quite different in the t (t) over barH signal and the dominant background (t (t) over barb (b) over bar).

Static detection of anomalies in transactional memory programs

Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para a obtenção do Grau de Mestre em Engenharia Informática

Algebraic structure for interaction on mixed models

Binary operations on commutative Jordan algebras, CJA, can be used to study interactions between sets of factors belonging to a pair of models in which one nests the other. It should be noted that from two CJA we can, through these binary operations, build CJA. So when we nest the treatments from one model in each treatment of another model, we can study the interactions between sets of factors of the first and the second models.

Pré-processamento e Tag automático de imagens em ambiente móvel e web, aplicado a um sistema de informação geográfica

No panorama socioeconómico atual, a contenção de despesas e o corte no financiamento de serviços secundários consumidores de recursos conduzem à reformulação de processos e métodos das instituições públicas, que procuram manter a qualidade de vida dos seus cidadãos através de programas que se mostrem mais eficientes e económicos. O crescimento sustentado das tecnologias móveis, em conjunção com o aparecimento de novos paradigmas de interação pessoa-máquina com recurso a sensores e sistemas conscientes do contexto, criaram oportunidades de negócio na área do desenvolvimento de aplicações com vertente cívica para indivíduos e empresas, sensibilizando-os para a disponibilização de serviços orientados ao cidadão. Estas oportunidades de negócio incitaram a equipa do projeto a desenvolver uma plataforma de notificação de problemas urbanos baseada no seu sistema de informação geográfico para entidades municipais. O objetivo principal desta investigação foca a idealização, conceção e implementação de uma solução completa de notificação de problemas urbanos de caráter não urgente, distinta da concorrência pela facilidade com que os cidadãos são capazes de reportar situações que condicionam o seu dia-a-dia. Para alcançar esta distinção da restante oferta, foram realizados diversos estudos para determinar características inovadoras a implementar, assim como todas as funcionalidades base expectáveis neste tipo de sistemas. Esses estudos determinaram a implementação de técnicas de demarcação manual das zonas problemáticas e reconhecimento automático do tipo de problema reportado nas imagens, ambas desenvolvidas no âmbito deste projeto. Para a correta implementação dos módulos de demarcação e reconhecimento de imagem, foram feitos levantamentos do estado da arte destas áreas, fundamentando a escolha de métodos e tecnologias a integrar no projeto. Neste contexto, serão apresentadas em detalhe as várias fases que constituíram o processo de desenvolvimento da plataforma, desde a fase de estudo e comparação de ferramentas, metodologias, e técnicas para cada um dos conceitos abordados, passando pela proposta de um modelo de resolução, até à descrição pormenorizada dos algoritmos implementados. Por último, é realizada uma avaliação de desempenho ao par algoritmo/classificador desenvolvido, através da definição de métricas que estimam o sucesso ou insucesso do classificador de objetos. A avaliação é feita com base num conjunto de imagens de teste, recolhidas manualmente em plataformas públicas de notificação de problemas, confrontando os resultados obtidos pelo algoritmo com os resultados esperados.

Detection and identification of dengue virus isolates from Brazil by a simplified reverse transcription - polymerase chain reaction (RT-PCR) method

We show here a simplified RT-PCR for identification of dengue virus types 1 and 2. Five dengue virus strains, isolated from Brazilian patients, and yellow fever vaccine 17DD as a negative control, were used in this study. C6/36 cells were infected and supernatants were collected after 7 days. The RT-PCR, done in a single reaction vessel, was carried out following a 1/10 dilution of virus in distilled water or in a detergent mixture containing Nonidet P40. The 50 µl assay reaction mixture included 50 pmol of specific primers amplifying a 482 base pair sequence for dengue type 1 and 210 base pair sequence for dengue type 2. In other assays, we used dengue virus consensus primers having maximum sequence similarity to the four serotypes, amplifying a 511 base pair sequence. The reaction mixture also contained 0.1 mM of the four deoxynucleoside triphosphates, 7.5 U of reverse transcriptase, 1U of thermostable Taq DNA polymerase. The mixture was incubated for 5 minutes at 37ºC for reverse transcription followed by 30 cycles of two-step PCR amplification (92ºC for 60 seconds, 53ºC for 60 seconds) with slow temperature increment. The PCR products were subjected to 1.7% agarose gel electrophoresis and visualized by UV light after staining with ethidium bromide solution. Low virus titer around 10 3, 6 TCID50/ml was detected by RT-PCR for dengue type 1. Specific DNA amplification was observed with all the Brazilian dengue strains by using dengue virus consensus primers. As compared to other RT-PCRs, this assay is less laborious, done in a shorter time, and has reduced risk of contamination

The friction force mouse-pad and the forearm muscles efforts (preliminary results)

The objective of the article is to evaluate the impact of the friction force mouse-pad in the contraction level of the forearm muscles M. extensor carpi ulnaris, M. extensor digitorum and M. extensor carpi radialis longus. A standard protocol of mouse movements was performed involving horizontal, vertical and diagonal mouse displacements drag-and-drop type. The operators were instructed to execute the protocol with their normal working speed. The movements protocol were performed by each subject (n=17) with three selected pairs mouse-pad, classified as low, medium and high friction force pairs. The mean time to execute the protocol with each mouse was ~138s. Mean values of ~13%MVE and ~17%MVE were found in the M. extensor carpi ulnaris and in the M. extensor digitorum respectively when performing the movements’ protocol. A 8.1% increase in %MVE was observed in the M. extensor digitorum and a 9.4% increase in %MVE was observed in the M. extensor carpi ulnaris when the high friction force pair was operated, relatively to the low friction force pair (p<0.05). The main preliminary conclusions of this study is that operating a high friction force mouse-pad may increase the risk to symptoms or disorders in the wrist due to an increase in the forearm muscles contraction levels, particularly during work with drawing application