Redefinición de las TIC en el museo: del discurso invasivo al inclusivo

Las TIC son inseparables de la museografía in situ e imprescindibles en la museografía en red fija y móvil. En demasiados casos se han instalado prótesis tecnológicas para barnizar de modernidad el espacio cultural, olvidando que la tecnología debe estar al servicio de los contenidos de manera que resulte invisible y perfectamente imbricada con la museografía tradicional. Las interfaces móviles pueden fusionar museo in situ y en red y acompañar a las personas más allá del espacio físico. Esa fusión debe partir de una base de datos narrativa y abierta a obras materiales e inmateriales de otros museos de manera que no se trasladen las limitaciones del museo físico al virtual. En el museo in situ tienen sentido las instalaciones hipermedia inmersivas que faciliten experiencias culturales innovadoras. La interactividad (relaciones virtuales) debe convivir con la interacción (relaciones físicas y personales) y estar al servicio de todas las personas, partiendo de que todas, todos tenemos limitaciones. Trabajar interdisciplinarmente ayuda a comprender mejor el museo para ponerlo al servicio de las personas.

The magical transformation contest in the ancient storytelling tradition

El concurso de transformación mágica, esquema narrativo difundido en la tradición popular, se presenta en dos variantes principales: los hechiceros que compiten pueden metamorfosearse en varios seres o crear esos seres por medios mágicos. En cualquier caso el concursante ganador da a luz criaturas más fuertes que superan las de su oponente. La segunda variante fue preferida en el antiguo Cercano Oriente (Sumeria, Egipto, Israel). La primera se puede encontrar en algunos mitos griegos sobre cambiadores de forma (por ejemplo, Zeus y Némesis). El mismo esquema narrativo puede haber influido en un episodio de la Novela de Alejandro (1.36-38), en el que Darío envía regalos simbólicos a Alejandro y los dos monarcas enemigos ofrecen contrastantes explicaciones de ellos. Esta historia griega racionaliza el concurso de cuento de hadas, transfiriendo las fantásticas hazañas de creaciones milagrosas a un plano secundario pero realista de metáfora lingüística.

Discrimination of honey of different floral origins by a combination of various chemical parameters

Abstract Honey is a high value food commodity with recognized nutraceutical properties. A primary driver of the value of honey is its floral origin. The feasibility of applying multivariate data analysis to various chemical parameters for the discrimination of honeys was explored. This approach was applied to four authentic honeys with different floral origins (rata, kamahi, clover and manuka) obtained from producers in New Zealand. Results from elemental profiling, stable isotope analysis, metabolomics (UPLC-QToF MS), and NIR, FT-IR, and Raman spectroscopic fingerprinting were analyzed. Orthogonal partial least square discriminant analysis (OPLS-DA) was used to determine which technique or combination of techniques provided the best classification and prediction abilities. Good prediction values were achieved using metabolite data (for all four honeys, Q² = 0.52; for manuka and clover, Q² = 0.76) and the trace element/isotopic data (for manuka and clover, Q² = 0.65), while the other chemical parameters showed promise when combined (for manuka and clover, Q² = 0.43).

Fast and sensitive aflatoxin B1 and total aflatoxins ELISAs for analysis of peanuts, maize and feed ingredients

Aflatoxins are a group of carcinogenic compounds produced by Aspergillus fungi that can grow on different agricultural crops. Both acute and chronic exposure to these mycotoxins can cause serious illness. Due to the high occurrence of aflatoxins in crops worldwide fast and cost-effective analytical methods are required for the identification of contaminated agricultural commodities before they are processed into final products and placed on the market. In order to provide new tools for aflatoxin screening two prototype fast ELISA methods: one for the detection of aflatoxin B₁ and the other for total aflatoxins were developed. Seven monoclonal antibodies with unique high sensitivity and at the same time good cross-reactivity profiles were produced. The monoclonal antibodies were characterized and two antibodies showing IC₅₀ of 0.037 ng/mL and 0.031 ng/mL for aflatoxin B₁ were applied in simple and fast direct competitive ELISA tests. The methods were validated for peanut matrix as this crop is one of the most affected by aflatoxin contamination. The detection capabilities of aflatoxin B₁ and total aflatoxins ELISAs were 0.4 μg/kg and 0.3 μg/kg for aflatoxin B₁, respectively, which are one of the lowest reported values. Total aflatoxins ELISA was also validated for the detection of aflatoxins B₂, G₁ and G₂. The application of the developed tests was demonstrated by screening 32 peanut samples collected from the UK retailers. Total aflatoxins ELISA was further applied to analyse naturally contaminated maize porridge and distiller's dried grain with solubles samples and the results were correlated with these obtained by UHPLC-MS/MS method.

Evaluación de la gestión escolar y tipo de escuelas en el Perú: una aplicación de costo efectividad

El presente documento tiene como objetivo evaluar alternativas de gestión y tamaños de colegios públicos en función de su contribución a la mejora del rendimiento de los estudiantes de primaria y al uso eficiente de los recursos públicos. Para tal fin, se utiliza la metodología de Análisis de Costo Efectividad (ACE), que consiste en seleccionar la mejor alternativa que brinde igual o mejores resultados en la población al menor costo, para lo cual se requiere definir un indicador de efectividad y calcular los costos de provisión de las dos o más alternativas.

Usos político-partidistas en cuentas de Twitter de administraciones públicas

Introducción: La comunicación institucional y la comunicación política deberían tener cauces de difusión separados pero, adoptando la hipótesis de la normalización, en las redes sociales también se refleja la tendencia al indebido uso partidista de los canales institucionales. Metodología: Este estudio analiza las publicaciones en Twitter de 40 cuentas institucionales españolas distribuidas equitativamente entre cuatro niveles territoriales (estatal, autonómico, provincial y local). Se trata de una investigación cualitativa con un método exploratorio en el que se ha empleado fundamentalmente el análisis de contenido. Resultados y conclusiones: Los resultados verifican la hipótesis de que se están dando usos propagandísticos y partidistas desde estas cuentas institucionales con una decena de tendencias que reflejan una gran preocupación por la proyección de los políticos en tanto que agentes de un partido. Por tanto, se consolida en este nuevo canal la confusión de estos dos planos, ya advertida en investigaciones previas con respecto a las salas de prensa online de una institución (García Orosa y Vázquez Sande, 2012).

Colour differentiation of high-lycopene tomato fruit through the addition of the colourless-epidermis (y) mutation

High-lycopene tomatoes (Solanum lycopersicum) are characterised by an intense red flesh-colour, due to an elevated concentration of the carotenoid, lycopene. However, this characteristic is only visible once fruit are cut open, making it impossible to differentiate intact high-lycopene fruit from standard tomato fruit, a clear market disadvantage. The reason that fruit colour of both high-lycopene and standard fruit looks almost identical from the outside is because tomato fruit normally contain the yellow flavonoid 'naringenin chalcone' in a thin layer of epidermal cells. It is this combination of naringenin chalcone and the underlying lycopene in the flesh that gives tomatoes their characteristic orange-red colour. By incorporation of the recessive colourless epidermis mutant allele 'y' (which prevents naringenin chalcone accumulation) into high-lycopene fruit, we have been able to create high-lycopene tomatoes (hp1.ogc.y) exhibiting a deep-pink colour visible from the outside. Hue angle of the skin of the high-lycopene 'y' mutant and a regular highlycopene tomato (hp1.ogc.Y) was 30 and 38°, respectively, while flesh values were similar at 31 and 32°, respectively. Removal of naringenin chalcone from the epidermis appeared to improve the visibility of underlying lycopene, such that fruit outer colour became a subsequent indicator of underlying flesh colour. The removal of epidermal pigmentation means that high-lycopene fruit can now be differentiated from standard tomato fruit in the market place without the need to cut fruit open.

The bacterial helicase-primase interaction: a common structural/functional module

The lack of a high-resolution structure for the bacterial helicase-primase complex and the fragmented structural information for the individual proteins have been hindering our detailed understanding of this crucial binary protein interaction. Two new structures for the helicase-interacting domain of the bacterial primases from Escherichia coli and Bacillus stearothermophilus have recently been solved and both revealed a unique and surprising structural similarity to the amino-terminal domain of the helicase itself. In this minireview, the current data are discussed and important new structural and functional aspects of the helicase-primase interaction are highlighted. An attractive structural model with direct biological significance for the function of this complex and also for the development of new antibacterial compounds is examined.

INFLACIÓN E INCERTIDUMBRE INFLACIONARIA: EVIDENCIA PARA COSTA RICA

En este documento se estima una medida de la incertidumbre inflacionaria. Un modelo de inflación señala incertidumbre cuando los errores de pronóstico son heteroscedásticos. Por medio de la especificación de una ecuación GARCH (Generalized Autoregressive Conditional Heteroscedasticity), para la varianza del término de error de un modelo de inflación, es posible estimar una proxy de incertidumbre inflacionaria. La estimación simultánea del modelo de inflación y de la ecuación GARCH, produce un nuevo modelo de inflación en el cual los errores de pronóstico son homocedásticos. Existe consenso en la literatura económica en que hay una correlación positiva entre incertidumbre inflacionaria y la magnitud de la tasa de inflación, lo cual, como lo señaló Friedman (1977), representa uno de los costos asociados con la persistencia inflacionaria. Esto es porque tal incertidumbre dificulta la toma de decisiones óptimas por parte de los agentes económicos.La evidencia empírica, para el periodo 1954:01-2002:08, apoya la hipótesis de que para el caso de Costa Rica mientras mayor es la inflación mayor es la incertidumbre respecto a esta variable. En los últimos siete años (1997-2002) la incertidumbre presenta la variación media más baja de todo el periodo. Además, se identifica un efecto asimétrico de la inflación sobre la incertidumbre inflacionaria, es decir, la incertidumbre inflacionaria tiende a incrementarse más para el siguiente periodo cuando la inflación pronosticada está por debajo de la inflación actual, que cuando la inflación pronosticada está por arriba de la tasa observada de inflación. Estos resultados tienen una clara implicación para la política monetaria. Para minimizar la dificultad que la inflación causa en la toma óptima de decisiones de los agentes económicos es necesario perseguir no solamente un nivel bajo de inflación sino que también sea estable.AbstractThis paper estimates a measure of inflationary uncertainty. An inflation model signals uncertainty when the forecast errors are heteroskedastic. By the specification of a GARCH (Generalized Autoregressive Conditional Heteroscedasticity) equation, for the variance of the error term of the inflation model, it is possible to estimate a proxy for inflationary uncertainty. By the simultaneous estimation of the inflation model and the GARCH equation, a new inflation model is obtained in which the forecast errors are homocedastic. Most economists agree that there is a positive correlation between inflationary uncertainty and the magnitude of the inflation rate, which, as was pointed out by Friedman (1977), represents one of costs associated with the persistence of inflation. This is because such uncertainty clouds the decision-making process of consumers and investors.The empirical evidence for the period 1954:01-2002:08 confirms that in the case of Costa Rica inflationary uncertainty increases as inflation rises. In the last seven years(1997-2002) the uncertainty present the mean variation most small of the period. In addition, inflation has an asymmetric effect on inflationary uncertainty. That is, when the inflation forecast is below the actual inflation, inflationary uncertainty increases for the next period. The opposite happens when the inflation forecast is above the observed rate of inflation. Besides, the absolute value of the change on uncertainty is greater in the first case than the second. These results have a clear implication for monetary policy. To minimize the disruptions that inflation causes to the economic decision-making process, it is necessary to pursue, not only a low level of inflation, but a stable one as well.

COMPETITIVIDAD DE LAS PyMES: ¿CÓMO PUEDEN LAS PEQUENAS Y MEDIANAS EMPRESAS DE COSTA RICA COMPETIR EN EL MERCADO LOCAL E INTERNACIONAL?

Muchas investigaciones han estudiado en los últimos años el tema de competitividad en las Pequeñas y Medianas Empresas conocidas como PyMES. Múltiples factores son los que explican la competitividad de éstas empresas, así como la competitividad de sectores productivos y naciones. En economías pequeñas como la de Costa Rica factores internos (empresa) tales como: limitada capacidad tecnológica, tradicionales técnicas de mercadeo, recurso humano poco calificado, incipiente gestión gerencial, entre otros, explican el bajo nivel competitivo de las PyMES. Además, un conjunto de factores externos como la política macroeconómica, conflictos y pugnas entre diferentes grupos sociales así como presencia de mercados imperfectos se han constituido en elementos condicionantes de la competitividad. El presente artículo ofrece un marco de referencia, el cual pretende clarificar el concepto de competitividad, desde diferentes referentes teóricos y sugiere modestos lineamientos estratégicos para el mejoramiento de la productividad, eficiencia y calidad de las PyMES en el mercado local e internacional.

Pore size distributions derived from adsorption isotherms, immersion calorimetry, and isosteric heats: A comparative study

We compare the pore size distribution of a well-characterized activated carbon derived from model-dependent, adsorption integral equation (AIE) methods with those from model-independent, immersion calorimetry and isosteric heat analyses. The AIE approach applied to nitrogen gave a mean pore width of 0.57 nm; the CO2 distribution exhibited wider dispersion. Spherical model application to CO2 and diffusion limitations for nitrogen and argon were proposed as primary reasons for inconsistency. Immersion enthalpy revealed a sharp decrease in available area equivalent to a cut-off due to molecular exclusion when the accessible surface was assessed against probe kinetic diameter. Mean pore width was identified as 0.58 ± 0.02 nm, endorsing the underlying assumptions for the nitrogen-based AIE approach. A comparison of the zero-coverage isosteric heat of adsorption for various non-polar adsorptives by the porous test sample was compared with the same adsorptives in contact with a non-porous reference adsorbent, leading to an energy ratio or adsorption enhancement factor. A linear relationship between the energy ratio and probe kinetic diameter indicated a primary pore size at 0.59 nm. The advantage of this enthalpy, model-independent methods over AIE were due to no assumptions regarding probe molecular shape, and no assumptions for pore shape and/or connectivity.

Prevalence of SOS-mediated control of integron integrase expression as an adaptive trait of chromosomal and mobile integrons

BACKGROUND Integrons are found in hundreds of environmental bacterial species, but are mainly known as the agents responsible for the capture and spread of antibiotic-resistance determinants between Gram-negative pathogens. The SOS response is a regulatory network under control of the repressor protein LexA targeted at addressing DNA damage, thus promoting genetic variation in times of stress. We recently reported a direct link between the SOS response and the expression of integron integrases in Vibrio cholerae and a plasmid-borne class 1 mobile integron. SOS regulation enhances cassette swapping and capture in stressful conditions, while freezing the integron in steady environments. We conducted a systematic study of available integron integrase promoter sequences to analyze the extent of this relationship across the Bacteria domain. RESULTS Our results showed that LexA controls the expression of a large fraction of integron integrases by binding to Escherichia coli-like LexA binding sites. In addition, the results provide experimental validation of LexA control of the integrase gene for another Vibrio chromosomal integron and for a multiresistance plasmid harboring two integrons. There was a significant correlation between lack of LexA control and predicted inactivation of integrase genes, even though experimental evidence also indicates that LexA regulation may be lost to enhance expression of integron cassettes. CONCLUSIONS Ancestral-state reconstruction on an integron integrase phylogeny led us to conclude that the ancestral integron was already regulated by LexA. The data also indicated that SOS regulation has been actively preserved in mobile integrons and large chromosomal integrons, suggesting that unregulated integrase activity is selected against. Nonetheless, additional adaptations have probably arisen to cope with unregulated integrase activity. Identifying them may be fundamental in deciphering the uneven distribution of integrons in the Bacteria domain.

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Combined media on photographic paper. 75" x 50"