Induction of intracellular cAMP by a synthetic retroviral envelope peptide: a possible mechanism of immunopathogenesis in retroviral infections.

A synthetic heptadecapeptide, CKS-17, represents the highly conserved amino acid sequences occurring within the transmembrane envelope protein of many animal and human retroviruses. CKS-17 has been demonstrated to exhibit suppressive properties for numerous immune functions. We have recently shown that CKS-17 acts as an immunomodulatory epitope causing an imbalance of human type 1 and type 2 cytokine production and suppression of cell-mediated immunities. cAMP, an intracellular second messenger, plays an important role in regulation of cytokine biosynthesis--i.e., elevation of intracellular cAMP levels selectively inhibits type 1 cytokine production but has no effect or enhances type 2 cytokine production. Here, we demonstrate that CKS-17 induces dramatic rises in the intracellular cAMP levels of a human monocyte cell line and of human peripheral blood mononuclear cells in a time- and dose-dependent manner. A peptide corresponding to the reverse sequence of CKS-17, used as control, has no effect on intracellular cAMP levels. The cAMP-inducing ability of CKS-17 is significantly blocked by SQ-22536, an inhibitor of adenylate cyclase. These results indicate that CKS-17, a highly conserved component of the transmembrane proteins of immunosuppressive retroviruses, induces increased intracellular levels of cAMP via activation of adenylate cyclase and suggest that this retroviral envelope peptide may differentially modulate type 1 and type 2 cytokine production through elevation of intracellular cAMP levels.

Differential modulation of Th1- and Th2-related cytokine mRNA expression by a synthetic peptide homologous to a conserved domain within retroviral envelope protein.

The influence of a synthetic retroviral peptide, CKS-17, on T helper type 1 (Th1)- or Th2-related cytokines was investigated in human blood mononuclear cells. Cells were stimulated with staphylococcal enterotoxin A, anti-CD3 plus anti-CD28 monoclonal antibodies, or lipopolysaccharide to induce cytokine mRNA. mRNA was detected by a reverse transcription-polymerase chain reaction or Northern blot analysis. CKS-17 down-regulated stimulant-induced mRNA accumulation for interferon gamma (IFN-gamma), interleukin (IL)-2, and p40 heavy and p35 light chains of IL-12, a cytokine that mediates development of Th1 response. CKS-17 up-regulated stimulant-induced mRNA accumulation of IL-10 and did not suppress Th2-related cytokine (IL-4, IL-5, IL-6, or IL-13) mRNA expression. A reverse sequence of CKS-17 peptide, used as a control, showed no such action. Anti-human IL-10 monoclonal antibody blocked ability of CKS-17 to inhibit mRNA accumulation for IFN-gamma but not the CKS-17 suppressive activity of IL-12 p40 heavy chain mRNA. Thus, CKS-17-mediated suppression of IFN-gamma mRNA expression is dependent upon augmentation of IL-10 production by CKS-17. This conserved component of several retroviral envelope proteins, CKS-17, may act as an immunomodulatory epitope responsible for cytokine dysregulation that leads to suppression of cellular immunity.

Terminology and French-Spanish business translation: evaluating terminology resources for the translation of accounting documents

This article is the English version of “Terminología y traducción económica francés-español: evaluación de recursos terminológicos en el ámbito contable” by Daniel Gallego Hernández. It was not published on the print version of MonTI for reasons of space. The online version of MonTI does not suffer from these limitations, and this is our way of promoting plurilingualism.

Guarantee of financial support for student James Savage, signed by William Tudor and John Cooper, 1799 July 25

This legal agreement, a guarantee of financial support for entering student James Savage (A.B. 1803), was signed on July 25, 1799 by his two guarantors, William Tudor and John Cooper. The document was also signed by two witnesses, William Tudor's sons John Henry Tudor and Frederic Tudor. The agreement specifies that, in the event of Savage's failure to settle all financial obligations to the President and Fellows of Harvard College during the course of his studies, the two guarantors would be responsible for a payment of two hundred ounces of silver. It seems that the Tudors and Cooper were relatives of Savage, thus explaining their desire to assure his entry to Harvard by entering into this financial obligation.

Plea in the case of Hamilton v. Saunders, 1763

Plea in response to the plaintiff's declaration. Signed: (Charles) Pinckney, defendant's attorney, and John Rutledge (plaintiff's attorney?). Note on verso: Filed 4th May 1763.

Nazi-deutsch, a glossary of contemporary German usage, with appendices on government, military and economic institutions,

"A revised and enlarged edition of the 'Dictionary of Nazi terms' compiled for the Office of European Economic Research."--Acknowledgments.

Realisation of absolute scale of temperature.

Reprinted from vol.1 of "The dictionary of applied physics."

The parting of the ways; or, Conquest by purchase.

Authorship attributed to Mrs. Bevan. cf. Kennedy's Dictionary of anonymous and pseudonymous English literature. 1926-

Twenty lessons in domestic science : a condensed home study course : marketing, food principals, functions of food, methods of cooking, glossary of usual culinary terms, pronunciations and definitions, etc. /

Some recipes use liquor as an ingredient.

Biographical directory of the American Congress, 1774-1961 : the Continental Congress, September 5, 1774, to October 21, 1788 and the Congress of the United States, from the First to the Eighty-sixth Congress, March 4, 1789, to January 3, 1961, inclusive.

"This volume, compiled by Clifford P. Reynolds, publications technician of the [Joint] Committee [on Printing], is a revision of the Dictionary of the United States Congress and the general government, published in 1859 and again revised in 1869, by Charles Lanman; the Biographical annals of the civil government of the United States in 1876, by Joseph Lanman and James Anglin, and the Lanman edition of 1876 as corrected by Joseph M. Morrison in 1887; the Political register and congressional directory of 1878; by Ben: Perley Poore; the Biographical congressional directory of 1903, by O. M. Enyart; the Biographical congressional directory in 1911, the Biographical directory of the American Congress of 1927, by Ansel Wold, and the 1949 edition by James L. Harrison."

The new DOT : a database of occupational titles for the twenty-first century : final report /

"May 1993"--Cover.

The makers of Canada,

[v. 15] Papineau, Cartier; by A.D. de Celles. [1904]--[v. 16] Egerton Ryerson, By N. Burwash. [1903] Selkirk, by Rev. G. Bryce. see Mackenzie.--[v. 17] John Graves Simcoe, by C. Scott. 1905.--[v. 18] Lord Sydenham, by A. Shortt. 1908. Simpson, by Rev. G. Bryce. see Mackenzie. Tilley, by J. Hannay. see Wilmot.--[v. 19] Wilmot and Tilley, by J. Hanney. 1907.--[v. 20] Wolfe and Montcalm, by the Abbé H.R. Casgrain. 1905.--[v. 21] Burpee, L.J. ed. Index and dictionary of Canadian history, ed. by L.J. Burpee ... and A.G. Doughty . 1911.

Marathi Bible dictionary.

Based on Bible dictionary of the American Sunday School Union of Philadelphia, and on Smith's Bible dictionary, but principally on Schaff's Dictionary of the Bible.--cf. Pref.

A sketch of the life of the late lieut.-colonel Champion, of the 95th regiment, with extracts from his corrsepondence.

"By various friends".--Dictionary of national biography.

The order of keeping a court leet & court baron, with the charges appertaining to the same (1650 ed.)

By John Kitchin. - cf. Dictionary of anonymous and pseudonymous English literature, by S. Halkett and J. Laing.