Utilização da berberina na identificação dos mastócitos do molusco Anomalocardia brasiliana

Berberine is an alkaloid used as a fluorochrome in the identification of heparin and DNA. Enerback, 1974, described the technique used until today to study granules rich in heparin of vertebrate mast cells. Santos et al., 2003, studied mast cells of the mollusk Anomalocardia brasiliana using biochemical and histological analysis. This work used the fluorescent dye berberine technique to improve characterization of these cells. Mollusk organs (ctenidium and mantle) were processed with routine histological techniques. Tissue sections were treated with berberine 0,02% in redistilled water acidified to pH 4, by the addition of citric acid for 20 minutes. The visualization was made through fluorescence microscopy with ultraviolet region emission. The mast cell fluorescence had a strong yellow color, where cell nuclei appeared more greenish. This result was very similar to the ones reported before. Mast cells are location at the epithelium surface is the same in both organs, mantle and ctenidium. The fluorescence was easily observed in the granules. Therefore, this technique showed to be good and sensitive to study mast cell of invertebrates

A gestão da informação sob a luz do Enterprise Content Management (ECM): um estudo de caso em uma universidade pública

This work involves the organization and content perspectives on Enterprise Content Management (ECM) framework. The case study at the Federal University of Rio Grande do Norte was based on ECM model to analyse the information management provided by the three main administrative systems: The Integrated Management of Academic Activities (SIGAA), Integrated System of Inheritance, and Contracts Administration (SIPAC) and the Integrated System for Administration and Human Resources (SIGRH). A case study protocol was designed to provide greater reliability to research process. Four propositions were examined in order to reach the specific objectives of identification and evaluation of ECM components from UFRN perspective. The preliminary phase provided the guidelines for the data collection. In total, 75 individuals were interviewed. Interviews with four managers directly involved on systems design were recorded (average duration of 90 minutes). The 70 remaining individuals were approached in random way in UFRN s units, including teachers, administrative-technical employees and students. The results showed the presence of many ECM elements in the management of UFRN administrative information. The technological component with higher presence was "management of web content / collaboration". But initiatives of other components (e.g. email and document management) were found and are in continuous improvement. The assessment made use of eQual 4.0 to examine the effectiveness of applications under three factors: usability, quality of information and offered service. In general, the quality offered by the systems was very good and walk side by side with the obtained benefits of ECM strategy adoption in the context of the whole institution

Perceções de comportamentos (Des)adequados e relacionamento com pares na escola: um estudo com alunos de 9º ano

Os comportamentos e as relações entre pares na escola são importantes para o desenvolvimento social e pessoal dos jovens. A influência exercida pelos pares pode ser positiva ou negativa. Esta investigação visa conhecer as perceções que os jovens têm sobre os comportamentos e relacionamentos adequados/desadequados entre pares. Participaram no estudo 16 estudantes do 9ºano, do sexo masculino, identificados como tendo bom/mau comportamento. A recolha dos dados foi realizada através de questionário constituído por questões abertas. Os dados foram tratados qualitativa e quantitativamente. Os bons e maus comportamentos são conceptualizados por relação com atitudes individuais e relacionais. A perceção dos professores sobre o comportamento dos jovens é diferente da perceção que estes têm do seu próprio comportamento. Os alunos consideram maioritariamente não serem influenciados pelos pares, nem exercerem influência sobre eles. A promoção do relacionamento entre pares é vista como passando pela realização de atividades especificas. O grupo de alunos identificado com mau comportamento apresenta menor variedade conceptual; ABSTRACT: The behaviors and relationships in peers at school are important to the social and personal development of young people. The influence exerted by peers can be positive or negative. This research aims to analyses the perception that young people have about the appropriate/inappropriate behaviors and relationships among peers. Sixteen male students of the 9thyear made part of this study, indentified with bad/good behavior. The data collection was made through questionnaires consisted of open questions. The data was evaluated qualitatively and quantitatively. The good and bad behaviors are conceptualized by individual and relational attitudes. The teachers’ perception of the behavior is different from the perception that the young people has of themselves. The majority of the students do not consider themselves as an influence to the peers, and not even the peers as an influence to them. The promotion of peer relationship is seen as through the realization of specific activities. The student group which was identified with bad behavior presents a lower conceptual variety.

A inclusão da criança com necessidades educativas especiais no jardim de infância

Dissertação de mest. em Ciências da Educação Especialização em Educação de Infância, Faculdade de Ciências Humanas e Sociais, Univ. do Algarve, 2005

Serum Hsp70 Antigen: Early Diagnosis Marker in Perinatal Asphyxia

Background: Perinatal asphyxia is an important cause of mortality and permanent neurological and developmental deficit. Early and accurate diagnosis would help to establish the likely prognosis and may also help in determining the most appropriate treatment. Studies in experimental animal models suggest that a protein called Hsp70 may be a good and potentially useful marker of cellular stress that may be clinically useful in determining the presence of neonatal asphyxia. Objectives: Regarding the importance of early and accurate diagnosis of asphyxia, we conducted this study, which is the first investigation of the comparison of the serum Hsp70 antigen level between asphyxiated and healthy infants. Patients and Methods: In this observational study, the serum concentrations of Hsp70 antigen were compared between neonates suffering from perinatal asphyxia (n = 50) and normal neonates (n = 51). The inclusion criteria for the cases were neonates who had reached term and had at least two clinical criteria of asphyxia. Exclusion criteria were babies with gestational age < 37 weeks, infants with congenital abnormalities or positive blood culture. Exclusion criteria in this group were the requirement to hospital stay during first week of the life or babies whose mothers had difficulties during pregnancy or delivery. Term neonates without major anomalies who had asphyxia during delivery were enrolled in the first six hours after delivery, and control group consisted of healthy term neonates without problems and normal delivery process in the first week of life. The cord blood was taken during labor to measure Hsp70 antigen level by using an in-house ELISA (The enzyme-linked immunosorbent assay). Results: The median values of serum anti Hsp70 titers were significantly higher in asphyxiated neonates compared with non-asphyxiated neonates (0.36 [0.04 - 1.14] vs 0.24 [0.01 - 0.63]). At cutoff point = 0.3125 ng/mL, sensitivity was 58% and specificity 76% based on ROC curve. Conclusions: A significant difference between the serum concentrations of Hsp70 of the control and patient group was observed in this study. It is inferred serum concentrations of Hsp70 antigen may be a useful marker for the early diagnosis of that prenatal hypoxia.

O papel da formação docente na efetividade das políticas públicas de EPT no Brasil - período 2003-2015 : implicações políticas e pedagógicas na atuação de professores

Tese (doutorado)—Universidade de Brasília, Faculdade de Educação, Programa de Pós-Graduação em Educação, 2016.

Is change in global self-rated health associated with change in affiliation with a primary care provider? Findings from a longitudinal study from New Zealand

To investigate the association of self-rated health and affiliation with a primary care provider (PCP) in New Zealand.
Methods

We used data from a New Zealand panel study of 22,000 adults. The main exposure was self-rated health, and the main outcome measure was affiliation with a PCP. Fixed effects conditional logistic models were used to control for observed time-varying and unobserved time-invariant confounding.
Results

In any given wave, the odds of being affiliated with a PCP were higher for those in good and fair/poor health relative to those in excellent health. While affiliation for Europeans increased as reported health declined, the odds of being affiliated were lower for Māori respondents reporting very good or good health relative to those in excellent health. No significant differences in the association by age or gender were observed.
Conclusions

Our data support the hypothesis that those in poorer health are more likely to be affiliated with a PCP. Variations in affiliation for Māori could arise for several reasons, including differences in care-seeking behaviour and perceived need of care. It may also mean that the message about the benefits of primary health care is not getting through equally to all population groups.

Dynamic price dispersion in Bertrand–Edgeworth competition

This paper studies a dynamic oligopoly model of price competition under demand uncertainty. Sellers are endowed with one unit of the good and compete by posting prices in every period. Buyers each demand one unit of the good and have a common reservation price. They have full information regarding the prices posted by each firm in the market; hence, search is costless. The number of buyers coming to the market in each period is random. Demand uncertainty is said to be high if there are at least two non-zero demand states that give a seller different option values of waiting to sell. Our model features a unique symmetric Markov perfect equilibrium in which price dispersion prevails if and only if the degree of demand uncertainty is high. Several testable theoretical implications on the distribution of market prices are derived.

Exploring the suburban ideal: Residents' experiences of photo elicitation interviewing (PEI)

This article reports on how photo elicitation interviewing (PEI) was experienced by eight participants in a qualitative phenom- enological study of suburban lived experiences. Adopting a respondent-controlled approach, participants were required to photograph at least 10 aspects of their suburb that they perceived to be good and problematical and then explain the meaning of their images as part of a follow-up interview. This discussion also involved in-depth reflection on the photography exercise itself, with analysis revealing that taking photographs was perceived to be a purposeful exercise that offered a range of benefits. It gave a new perspective and appreciation for the community, while also helping to ensure their meanings were conveyed to the inter- viewers. The photography task was not without challenges. While some of these challenges influenced the images captured, the findings of this study highlight the importance for qualitative researchers who use PEI to not only consider the benefits and limitations for the research but also give due consideration to the experiences of the participant photographers.

Setting the record straight: assessing the reliability of retrospective accounts of change

Conservation Letters published by Wiley Periodicals, Inc. Ecological degradation is accelerating, reducing our ability to detect and reverse declines. Resource user accounts have the potential to provide critical information on past change but their reliability can rarely be tested, hence they are often perceived as less valid than other forms of scientific data. We compared individual fishers' catch records, recorded 1-50 years ago, with their memories of past good, typical and poor catches for the corresponding time period. Good and poor catches were recalled with reasonable accuracy, matching variability in recorded catch with no significant change observed over time. Typical recalled catches were overestimated and became significantly more exaggerated over time, but were more comparable to mean than median recorded values. While accuracy of resource users' memory varied with the type of information recalled, our results suggest that carefully structured interview questions can produce reliable quantitative data to inform resource management, even after several decades have elapsed.

El fenotipo "basal-her2" en el cáncer de mama: caracterización clínico-molecular e implicaciones terapéuticas

Clinically HER2+ (cHER2+) breast cancer (BC), as exclusively determined by immunohistochemistry of HER2 protein overexpression and/or fluorescence in situ hybridization of HER2 gene amplification, has been largely considered a single disease entity in terms of clinical outcome and in the susceptibility to the anti-HER2 monoclonal antibody trastuzumab (Herceptin). However, although the adjuvant/neoadjuvant use of the trastuzumab has been shown to significantly reduce recurrence risk when added to standard chemotherapy in women with early-stage cHER2+ BC, not all cases derive similar benefit from trastuzumab because a significant number of cHER2+ BC patients develop disease recurrence. Unfortunately, the identification of a robust clinical predictor of trastuzumab benefit, including HER2 itself, has proven challenging in the adjuvant/neoadjuvant setting. Thus, we suggest that a new generation of research needs to refine the prognostic taxonomy of cHER2+ BC and develop easy-to-use, clinicbased prediction algorithms to distinguish between good- and poor- responders to trastuzumab-based therapy ab initio. This study offered two hypotheses: 1.) HER2 overexpression can unexpectedly take place in a molecular background owned by basal-like BC (a commonly HER2-negative BC subtype which possesses many epithelial-mesenchymal transition (EMT) characteristics and exhibits robust cancer stem cell [CSC]-like features), thus generating a so-called basal/cHER2+ BC subtype; 2.) the basal/cHER2+ phenotype confers poor prognosis and delineates a subgroup of intrinsically aggressive cHER2+ BC with primary resistance to trastuzumab...

Florescimento psicológico e sentido navida: que relações: Validação preliminar da escala MHC-SF

Dissertação de Mestrado apresentada no ISPA – Instituto Universitário para obtenção do grau de Mestre em Psicologia especialidade em Psicologia da Saúde.

Fundamentos do agir ético em estudantes do ensino superior

Introduction: It is urgent that higher education in health develop a proactive ethico-moral commitment in students which is translated into the creation of socio-professional values. Objectives: To evaluate the ethical foundations which support morality in higher education students. Methods: A descriptive, cross-sithectional study, performed in 345 ESSV/IPV students, 80% female, average age of 20.82 years old. The “Questionário de Cidadania Ativa e Modo de Agir Ético” (CiAMAE) by Cunha (2015) was applied. Results: The results were 74.8% of the students showed a positive/adequate ethical method of acting; (with 45.8% adequate and 29% very adequate). In 25.2% the method of acting was ethically inadequate. The morality of the student’s actions were focused mostly on individualism and were based on the following ethical principles: 68.7% ethical subjectivism (69.1%♂ and 68.6%♀); 56.5% Relativism ( 56.5%♂ and 56.7%♀); 53.9% Deontological Ethics (Kant) (55.8%♂ and 47.0%♀); 11.3% Subjectivism/Ethical Selfishness (7.4%♂ and 12.3%♀). Conclusions: We may infer that the majority of the students do not have/ do not use an impartial consideration of good as criteria for morality of actions. They mostly manifested accepting an ethical subjectivist perspective, which means that good is not considered as a value for everyone. In contrast, applying the criteria of ethical impartiality would imply considering the greater good, and choosing/adopting the universal point of view in which any rational and enlightened student would choose for him/ herself and for others that universal greater good.

Inclusão, Direitos Humanos e Igualdade

O presente estudo denominado “Inclusão, Direitos Humanos e Igualdade: Educar para a diferença” direcionou-se para a temática da deficiência física evidente. Partindo do mestrado em Ação Humanitária, Cooperação e Desenvolvimento, incidimos o nosso estudo na temática dos direitos humanos associada à da inclusão. A diferença, usualmente, origina exclusão em virtude de que o que é diferente não é socialmente aceite. Vivemos numa sociedade formatada para o “normal” em que o “normal” apresenta-se sempre como um modo de supressão gradual da diferença e da uniformização da diversidade e onde dificilmente encaixa a “diferença”. Uma das áreas onde se verifica tratamento diferenciado é a nível da deficiência que, não sendo entendida pela sociedade, gera um ciclo vicioso que dificilmente se quebra. Para tal, a escola terá de desempenhar um papel de extrema importância, modificando mentalidades, promovendo a deficiência, para que no futuro esta não seja encarada como uma diferença, mas como uma mais-valia no processo de singularidade e de diversidade humanas. Os comportamentos e os movimentos de transformação não podem ser impostos, mas devem ser introduzidos, compreendidos e modificados, pois só desta forma podem servir como alavanca de suporte para uma sociedade mais justa, mais inclusiva, mais humana, pois está nas mãos de cada um de nós, educadores, formar futuros cidadãos conscientes, ativos e responsáveis. A sociedade atual vive momentos conturbados decorrentes de interesses geopolíticos e estratégicos que potenciam conflitos armados, em que cada vez mais a população é indiscriminadamente afetada. Uma das consequências destes conflitos é a deficiência física evidente, aqui explorada, e que é uma realidade inerente à nossa prática profissional. Todos os dias são colocados em questão os direitos humanos de quem tem que conviver diariamente com ambientes bélicos. Apesar da sociedade portuguesa estar pouco desperta para esta realidade, pareceu-nos pertinente levar esta temática para a escola tentando explorar as perceções de crianças face à deficiência física evidente, quando observada noutras crianças, em ambiente escolar. Fizemos a aplicação de diversos métodos (inquéritos, visualização de imagens e vídeo, atividades de simulação de deficiência), atividades práticas em ambiente escolar que nos permitissem atingir o nosso objetivo geral. Posteriormente, foi realizada a interpretação de dados decorrentes da aplicação da metodologia, quer quantitativamente, quer qualitativamente efetuada a sua análise e retiradas conclusões.

Levnadsvanor och självskattad hälsa hos 40-åringar

Självskattad hälsa beskriver personens subjektiva uppfattning av sin hälsa. En lågt självskattad hälsa har samband med ökad framtida dödlighet. Ett starkt välbefinnande är enligt Katie Erikssons dimensioner av hälsa, förutsättningen för att hälsan ska skattas som bra. Syftet med denna studie var att beskriva eventuella skillnader i självskattad hälsa sett till olika levnadsvanor hos 40-åriga kvinnor och män. Metod: Enkätsvar från totalt 1144 40-åriga kvinnor och män har använts. Enkäterna genomfördes under 2014 på 55 vårdcentraler i två regioner i södra Sverige. Levnadsvanor som valdes till denna studie var fysisk aktivitet, grönsaks- och frukostvanor, alkoholintag, rökning, sysselsättning, sömn och stress. Självskattad hälsa kategoriserades som bra (”mycket bra” och ”bra”) och dålig (”någorlunda”, ”dålig” och ”mycket dålig”). För att studera skillnader användes Student T-test på parametrisk data och Chi-två på icke parametrisk data. Resultat: De levnadsvanor som var vanligare förekommande hos de med bra självskattad hälsa var ansträngande/hård motion, låg nivå av stress och att vara i arbete (p<.001). Att inte röka hos män och att äta frukost och grönsaker hos kvinnor var också vanligare förekommande hos de med bra självskattad hälsa. Lågt intag av alkohol visade inte på några skillnader.  Slutsats: För att minska risken för framtida sjuklighet är det viktigt för distriktssköterskor, samt flera andra samhällsinsatser, att försöka påverka och uppmuntra hög fysisk aktivitet och minskad stress.