Generation of aldehyde-derived protein modifications in ethanol-exposed heart

Background: Although excessive ethanol consumption is known to lead to a variety of adverse effects in the heart, the molecular mechanisms of such effects have remained poorly defined. We hypothesized that posttranslational covalent binding of reactive molecular species to proteins occurs in the heart in response to acute ethanol exposure. Methods: The generation of protein adducts with several aldehydic species was examined by using monospecific antibodies against adducts with malondialdehyde (MDA), acetaldehyde (AA), MDA-AA hybrids, and hydroxyethyl radicals. Specimens of heart tissue were obtained from rats after intraperitoneal injections with alcohol (75 mmol/kg body weight) with or without pretreatment with cyanamide (0.05 mmol/kg body weight), an aldehyde dehydrogenase inhibitor. Results: The amounts of MDA and unreduced AA adducts were found to be significantly increased in the heart of the rats treated with ethanol, cyanamide, or both, whereas no other adducts were detected in statistically significant quantities. Immunohistochemical studies for characterization of adduct distribution revealed sarcolemmal adducts of both MDA and AA in the rats treated with ethanol and cyanamide in addition to intracellular adducts, which were also present in the group treated with ethanol alone. Conclusions: These findings support the role of enhanced lipid peroxidation and the generation of protein-aldehyde condensates in vivo as a result of excessive ethanol intake. These findings may have implications in the molecular mechanisms of cardiac dysfunction in alcoholics.

Avalia????o de pol??ticas de financiamento a micro e pequenas empresas na gera????o de postos de trabalho: o caso do Proger ??? principais aspectos

O artigo se prop??e a apresentar os principais aspectos e resultados de uma nova metodologia de avalia????o de programas de financiamento a micro e pequenas empresas aplicada ao Programa de Gera????o de Emprego e Renda na ??rea urbana ??? Proger urbano, tanto no que se refere aos seus custos efetivos, at?? ent??o desconhecidos, quanto no que se refere ao seu impacto sobre a gera????o de empregos, estimado utilizando dados prim??rios oriundos do cruzamento de dois bancos de dados do Minist??rio do Trabalho e Emprego, quais sejam, o do Sistema de Acompanhamento da Execu????o do Proger (Saep), e o do Cadastro Geral de Empregados e Desempregados. S??o apresentados, preliminarmente, alguns dos resultados de duas avalia????es anteriores do programa, uma realizada pelo Ibase, atrav??s de question??rios aos benefici??rios do programa, e outra, uma primeira experi??ncia de cruzamento dos dois referidos bancos de dados, que serviu de base para a avalia????o que se apresenta.

A practical framework for the generation and selection of strategic plans of action, The relevance of timeframe considerations and a systems thinking approach

The paper proposes a methodology especially focused on the generation of strategic plans of action, emphasizing the relevance of having a structured timeframe classification for the actions. The methodology explicitly recognizes the relevance of long-term goals as strategic drivers, which must insure that the complex system is capable to effectively respond to changes in the environment. In addition, the methodology employs engineering systems techniques in order to understand the inner working of the system and to build up alternative plans of action. Due to these different aspects, the proposed approach features higher flexibility compared to traditional methods. The validity and effectiveness of the methodology has been demonstrated by analyzing an airline company composed by 5 subsystems with the aim of defining a plan of action for the next 5 years, which can either: improve efficiency, redefine mission or increase revenues.

The main benefits associated with health and safety management systems certification in Portuguese small and medium enterprises post quality management system certification

The purpose of this study is to characterize how Portuguese Small and Medium Enterprises (SMEs) view the Occupational Health and Safety Management Systems (OHSMSs) certification process, after receiving the Quality Management System (QMS) certification. References were based on the ISO 9001 standard for a QMS and OHSAS 18001 for OHSMS. The method used to evaluate the implemented systems, was by form of questionnaire. Those questioned had to have a certified quality management system, an implemented OHSMS and be a SME. The questionnaire was sent to 300 SMEs; 46 responses were received and validated. Of them, only 12 SMEs had the OHSMS certificate according to OHSAS 18001. Within those 12 companies that participated: 7 SMEs are from the industrial sector; 3 belong to the electricity/telecommunications sector and 2 SMEs are from the trade/services activity sector. The size of the sample was small, but corresponds to Portuguese reality. Moreover, 34 SMEs did not have the OHSMS certificate. The questionnaire requested the main reasons for SMEs to opt for non-certification and it was related with high costs, while the main reasons to certificate were, among others, needed to eliminate or minimize risks to workers. The main benefits that Portuguese SMEs have gained from the referred certifications have been, improved working conditions, ensuring compliance with legislation and better internal communication about risks and hazards. Also presented are the main difficulties in achieving an OHSMS certification including high certification costs, difficulties motivating personnel, difficulties in changing the company’s culture and increased bureaucracy.

Certification and integration of management systems: the experience of Portuguese small and medium enterprises

The purpose of this study was to characterize the situation of Portuguese Small and Medium Enterprises (SMEs) concerning the certification of their Quality Management Systems (QMS), Environmental Management Systems (EMS) and Occupational Health and Safety Management Systems (OHSMS), in their individually form, to identify benefits, drawbacks and difficulties associated with the certification process and to characterize the level of integration that has been achieved. This research was based on a survey carried out by the research team; it was administered to 46 Portuguese SMEs. Our sample comprised 20 firms (43%) from the Trade/Services activity sector, 17 (37%) from the Industrial sector, 5 (11%) from the Electricity/Telecommunications sector and 4 (9%) from the Construction area. All SMEs surveyed were certified according to the ISO 9001 (100%), a quarter of firms were certified according to the ISO 14001 (26.1%) and a few certified by OHSAS 18001 (15.2%). We undertook a multivariate cluster analysis, which enabled grouping variables into homogeneous groups or one or more common characteristics of the SMEs participating in the study. Results show that the main benefits that Portuguese SMEs have gained from the referred certifications have been, among others, an improvement of both their internal organization and external image. We also present the main difficulties in achieving certification. Overall, 7 of the Portuguese SMEs examined indicated that the main benefits of the IMS implementation management included costs reduction, increased employee training and easier compliance of legislation. The respective drawbacks and difficulties are also presented. Finally, we presented the main integrated items in the certified Portuguese SMEs we examined.

Uma Abordagem para a Geração de Casos de Teste Baseada em Modelos

Abstract — The analytical methods based on evaluation models of interactive systems were proposed as an alternative to user testing in the last stages of the software development due to its costs. However, the use of isolated behavioral models of the system limits the results of the analytical methods. An example of these limitations relates to the fact that they are unable to identify implementation issues that will impact on usability. With the introduction of model-based testing we are enable to test if the implemented software meets the specified model. This paper presents an model-based approach for test cases generation from the static analysis of source code.

Performance, carcass traits and costs of Suffolk lambs finishing systems with early weaning and controlled suckling

The performance, carcass traits and finishing costs of Suffolk lambs were evaluated in three systems: (1) lambs weaned with 22 kg of body weight (BW) and supplemented with concentrate on pasture until slaughter; (2) lambs weaned with 22 kg BW and fed in feedlot until slaughter; (3) lambs maintained in controlled nursing after 22 kg BW and creep fed in feedlot until slaughter. Average daily gain (ADG) was 224 g/d for lambs weaned and supplemented with concentrate on pasture, 386 g/d for lambs weaned in feedlot and 481 g/d for lambs under controlled nursing. Empty body weight and visceral fat deposition were highest in lambs from feedlot systems. Carcass weights and carcass yields were highest for lambs in controlled nursing. Finishing total costs were highest in controlled nursing and lowest in the system with weaning in feedlot. High concentrate diet associated with controlled nursing in feedlot allowed lambs to reach the growth potential and carcasses with higher weights, higher yields and higher fat content. After weaning, lambs in feedlot fed with high concentrate diet had higher weight gain than lambs supplemented with concentrate on pasture. Carcasses produced under these two systems presented the same characteristics. The system with weaning in feedlot showed the lowest cost per kg carcass.

Transaction costs: an empirical analysis of their relationship with investment and foreign direct investment

As stated by the New Institutional Economics theory, transaction costs play a relevant role in economics and, according to the extent of such costs, agents make investment decisions. Actually, transaction costs may represent a disincentive to entrepreneurship. This work aims to verify whether transaction costs are related to investment rate and foreign direct investment rate (FDI) in different business environments. The results suggest that foreign investors do not have precise information about other countries as domestic investors do; as it is observed, only the relation between transaction costs and investment rate is significant. Furthermore, there is evidence that the business environments of BRIC countries are less developed when compared to business environments of other countries in the study

(Virtual) Agents for running electricity markets

This paper describes a multi-agent based simulation (MABS) framework to construct an artificial electric power market populated with learning agents. The artificial market, named TEMMAS (The Electricity Market Multi-Agent Simulator), explores the integration of two design constructs: (i) the specification of the environmental physical market properties and (ii) the specification of the decision-making (deliberative) and reactive agents. TEMMAS is materialized in an experimental setup involving distinct power generator companies that operate in the market and search for the trading strategies that best exploit their generating units' resources. The experimental results show a coherent market behavior that emerges from the overall simulated environment.

A flexible side information generation framework for distributed video coding

One of the most efficient approaches to generate the side information (SI) in distributed video codecs is through motion compensated frame interpolation where the current frame is estimated based on past and future reference frames. However, this approach leads to significant spatial and temporal variations in the correlation noise between the source at the encoder and the SI at the decoder. In such scenario, it would be useful to design an architecture where the SI can be more robustly generated at the block level, avoiding the creation of SI frame regions with lower correlation, largely responsible for some coding efficiency losses. In this paper, a flexible framework to generate SI at the block level in two modes is presented: while the first mode corresponds to a motion compensated interpolation (MCI) technique, the second mode corresponds to a motion compensated quality enhancement (MCQE) technique where a low quality Intra block sent by the encoder is used to generate the SI by doing motion estimation with the help of the reference frames. The novel MCQE mode can be overall advantageous from the rate-distortion point of view, even if some rate has to be invested in the low quality Intra coding blocks, for blocks where the MCI produces SI with lower correlation. The overall solution is evaluated in terms of RD performance with improvements up to 2 dB, especially for high motion video sequences and long Group of Pictures (GOP) sizes.

Scheduling of head-dependent cascaded reservoirs considering discharge ramping constraints and start/stop of units

This paper is on the problem of short-term hydro scheduling (STHS), particularly concerning head-dependent reservoirs under competitive environment. We propose a novel method, based on mixed-integer nonlinear programming (MINLP), for optimising power generation efficiency. This method considers hydroelectric power generation as a nonlinear function of water discharge and of the head. The main contribution of this paper is that discharge ramping constraints and start/stop of units are also considered, in order to obtain more realistic and feasible results. The proposed method has been applied successfully to solve two case studies based on Portuguese cascaded hydro systems, providing a higher profit at an acceptable computation time in comparison with classical optimisation methods based on mixed-integer linear programming (MILP).

Utilização racional de energia eléctrica no município de Vila Franca de Xira: casos de estudo

O objectivo pretendido alcançar é o de obter uma redução nos consumos de energia eléctrica do Município de Vila Franca de Xira, recorrendo à URE. Esta URE consiste num conjunto de acções e/ou medidas e/ou procedimentos e/ou equipamentos cuja aplicação tem como fim, o de potenciar uma utilização e uma gestão mais racional e rentável da energia - daí se podendo dizer, cada vez mais, que a mesma é um factor essencial de economia energética, logo, de redução de custos. A aplicação destas acções e/ou medidas e/ou procedimentos e/ou equipamentos deverá, por isso, ser extensível aos Municípios, com o fim de fazer reduzir uma factura energética de grande peso e significado, a qual, em tempos que são de profunda crise económica, pode tornar problemático ou até mesmo hipotecar, o respectivo futuro. Assim e se por um lado existe no Município um conjunto alargado de situações estabelecidas sem critérios de racionabilidade energética e ás quais, todavia, é já possível fazer aplicar essas acções e/ou medidas e/ou procedimentos e/ou equipamentos de URE, por outro lado, é daí garantido que se poderá alcançar a pretendida redução dos consumos energéticos do Município, sempre assegurando e mantendo o conforto e a produtividade das actividades dependentes dessa energia. Com esse objectivo e partindo da análise de um conjunto de instalações e/ou equipamentos já existentes ou com possibilidades de virem a ser estabelecidas/os pelo Município, é pretendido definir, estudar e classificar, um conjunto de dados que tendam a desenvolver, potenciar e justificar a decisão da sua aplicação. Um conjunto alargado de exemplos práticos ou Casos de Estudo serão desenvolvidos, tentando chegar a conclusões sobre a viabilidade económica das soluções apresentadas e, daí, da decisão da oportunidade da execução do conjunto de trabalhos inerentes à aplicação desse tipo de situação.

A gestão do risco associado à negociação de energia eléctrica através de contratos bilaterais e mercado spot

Num mercado de electricidade competitivo onde existe um ambiente de incerteza, as empresas de geração adoptam estratégias que visam a maximização do lucro, e a minimização do risco. Neste contexto, é de extrema importância para desenvolver uma estratégia adequada de gestão de risco ter em conta as diferentes opções de negociação de energia num mercado liberalizado, de forma a suportar a tomada de decisões na gestão de risco. O presente trabalho apresenta um modelo que avalia a melhor estratégia de um produtor de energia eléctrica que comercializa num mercado competitivo, onde existem dois mercados possíveis para a transacção de energia: o mercado organizado (bolsa) e o mercado de contratos bilaterais. O produtor tenta maximizar seus lucros e minimizar os riscos correspondentes, seleccionando o melhor equilíbrio entre os dois mercados possíveis (bolsa e bilateral). O mercado de contratos bilaterais visa gerir adequadamente os riscos inerentes à operação de mercados no curto prazo (mercado organizado) e dar o vendedor / comprador uma capacidade real de escolher o fornecedor com que quer negociar. O modelo apresentado neste trabalho faz uma caracterização explícita do risco no que diz respeito ao agente de mercado na questão da sua atitude face ao risco, medido pelo Value at Risk (VaR), descrito neste trabalho por Lucro-em-Risco (PAR). O preço e os factores de risco de volume são caracterizados por um valor médio e um desvio padrão, e são modelizados por distribuições normais. Os resultados numéricos são obtidos utilizando a simulação de Monte Carlo implementado em Matlab, e que é aplicado a um produtor que mantém uma carteira diversificada de tecnologias de geração, para um horizonte temporal de um ano. Esta dissertação está organizada da seguinte forma: o capítulo 1, 2 e 3 descrevem o estado-da-arte relacionado com a gestão de risco na comercialização de energia eléctrica. O capítulo 4 descreve o modelo desenvolvido e implementado, onde é também apresentado um estudo de caso com uma aplicação do modelo para avaliar o risco de negociação de um produtor. No capítulo 5 são apresentadas as principais conclusões.

Estudo técnico/económico da instalação de uma central de cogeração numa serração de madeira

O pinheiro tem um papel importante na ecologia e economia nacional. O Pinheiro sofre de uma praga severa, denominada por doença da murchidão dos pinheiros, causada pelo nemátodo da madeira do pinheiro (NMP). Apresenta-se como um verme microscópico, invertebrado, medindo menos de 1,5 mm de comprimento. O contágio entre árvores deve-se a vectores biologicamente conhecidos por longicórneo e capricórnio do pinheiro. Os produtores de madeira de pinho são desta forma obrigados a efectuar tratamentos térmicos (HT), de eliminação do NMP e dos seus vectores para que a exportação da madeira serrada cumpra com a norma NP 4487. De modo a manter a competitividade internacional das empresas nacionais, o impacto dos custos do HT deve ser minimizado. O objectivo desta dissertação é efectuar o estudo técnico-económico da implementação de um sistema de cogeração capaz produzir calor para efectuar o tratamento ao NMP e simultaneamente energia eléctrica para vender à rede pública. As receitas da venda de energia eléctrica poderão contribuir para a minimização dos custos do HT. Tendo em conta que os resíduos das serrações de madeira podem ser usados como combustível consideraram-se para avaliação duas tecnologias de cogeração, um sistema de turbina a vapor clássico (ciclo Rankine) e um sistema Organic Rankine Cycle (ORC), permitindo ambas a queima dos resíduos das serrações de madeira. No que diz respeito à avaliação económica, foi desenvolvido um simulador de tecnologia/modalidade de remuneração que efectua cálculos consoante as necessidades térmicas de cada produtor, a potência eléctrica a instalar e indicadores económicos, VAL, TIR e PAYBACK da instalação do sistema de cogeração. O simulador desenvolvido aplica a nova legislação que enquadra o sistema jurídico e remuneratório da cogeração (DL 23/2010), na qual se consideram duas modalidades, geral e especial. A metodologia desenvolvida foi aplicada num caso real de uma serração de madeira e os principais resultados mostram que as soluções apresentadas, turbina a vapor e sistema ORC, não apresentam viabilidade económica. Através da análise de sensibilidade, conclui-se que um dos factores que mais influência a viabilidade económica do projecto é o tempo de funcionamento reduzido. Sendo uma das soluções apresentada a criação de uma central de cogeração para vários produtores de madeira. Uma possível solução para o problema do reduzido tempo de utilização seria o fornecimento do serviço de tratamentos térmicos a outros produtores de paletes de madeira que não possuem estufa própria.

Nonlinear optimization method for short-term hydro scheduling considering head-dependency

This paper is on the problem of short-term hydro scheduling, particularly concerning head-dependent reservoirs under competitive environment. We propose a new nonlinear optimization method to consider hydroelectric power generation as a function of water discharge and also of the head. Head-dependency is considered on short-term hydro scheduling in order to obtain more realistic and feasible results. The proposed method has been applied successfully to solve a case study based on one of the main Portuguese cascaded hydro systems, providing a higher profit at a negligible additional computation time in comparison with a linear optimization method that ignores head-dependency.