Trade-off aproaches for the vibroacoustic analysis of trains

Passengers’ comfort in terms of acoustic noise levels is a key design driver for train design. The problem is especially relevant for high speed trains, where the aerodynamic induced noise is dominant, but it is also important for medium speed trains where the mechanical sources of noise may have more influence. The numerical interior noise prediction inside the train is a very comp lex problem, involving many different parameters: complex geometries and materials, different noise sources, com- plex interactions among those sources, broad range of frequencies where the phenomenon is important, etc. In this paper, the main findings of this work developed at IDR/UPM (Instituto de Microgravedad “Ignacio Da Riva”, Universidad Politécnica de Madrid) are presented, concentrat ing on the different modelling methodologies used for the different frequency ranges of interest, from FEM-BEM models, hybrid FEM-SEA to pure SEA models. The advantages and disadvantages of the different approaches are summarized. Different modelling techniques have also been evaluated and compared, taking into account the various and specific geometrical configurations typical in this type of structures, and the material properties used in the models. The critical configuration of the train inside a tunnel is studied in order to evaluate the external loads due to noise sources of the train. In this work, a SEA-model composed by periodic characteristic sections of a high spee d train is analysed inside a tunnel.

Expression cloning of cDNA encoding a human β-1,3-N-acetylglucosaminyltransferase that is essential for poly-N-acetyllactosamine synthesis

The structure and biosynthesis of poly-N-acetyllactosamine display a dramatic change during development and oncogenesis. Poly-N-acetyllactosamines are also modified by various carbohydrate residues, forming functional oligosaccharides such as sialyl Lex. Herein we describe the isolation and functional expression of a cDNA encoding β-1,3-N-acetylglucosaminyltransferase (iGnT), an enzyme that is essential for the formation of poly-N-acetyllactosamine. For this expression cloning, Burkitt lymphoma Namalwa KJM-1 cells were transfected with cDNA libraries derived from human melanoma and colon carcinoma cells. Transfected Namalwa cells overexpressing the i antigen were continuously selected by fluorescence-activated cell sorting because introduced plasmids containing Epstein–Barr virus replication origin can be continuously amplified as episomes. Sibling selection of plasmids recovered after the third consecutive sorting resulted in a cDNA clone that directs the increased expression of i antigen on the cell surface. The deduced amino acid sequence indicates that this protein has a type II membrane protein topology found in almost all mammalian glycosyltransferases cloned to date. iGnT, however, differs in having the longest transmembrane domain among glycosyltransferases cloned so far. The iGnT transcript is highly expressed in fetal brain and kidney and adult brain but expressed ubiquitously in various adult tissues. The expression of the presumed catalytic domain as a fusion protein with the IgG binding domain of protein A enabled us to demonstrate that the cDNA encodes iGnT, the enzyme responsible for the formation of GlcNAcβ1 → 3Galβ1 → 4GlcNAc → R structure and poly-N-acetyllactosamine extension.

O pluralismo jurídico na constituição de 1988 / Silvio Dobrowolski. --

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Novas funções e estrutura do poder judiciário na Constituição de 1988 : uma introdução / Silvio Dobrowolski. --

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A constituição de 1988 : um mito para resgatar / Silvio Dobrowolski. --

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A Formação e o aperfeiçoamento do magistrado contemporâneo

Estudo comparado sobre o tratamento dado a seleção e aperfeiçoamento da magistratura no direito dos seguintes países : Coreia, Alemanha, Estados Unidos, França, Portugal, Espanha e Itália

O magistrado e o ideal de justiça / Reis Friede. --

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O novo perfil da liqüidação de sentença / Edilson Pereira Nobre Júnior. --

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