964 resultados para Português Não-universitário (K-12)
Resumo:
Com o objetivo de determinar: - a curva de crescimento do melão (Cucumis melo L.), através da acumulação de matéria seca; - os teores e consequentes acúmulos de nutrientes nos órgãos aéreos da planta, em diferentes estádios de crescimento; - a exportação de nutrientes na colheita de frutos, no ponto de consumo. Foi conduzido um ensaio em condições de campo o qual consistiu da amostragem em cinco estádios de crescimento - 15, 30, 45, 60 e 75 dias após a emergência - de plantas competitivas, as quais eram cortadas rente ao solo, divididas em caule e ramos, folhas, flores e frutos, para determinação de quantidade de matéria seca e análise química para macronutrientes minerais. Os tratamentos, então representados por épocas de amostragem, constaram de um delineamento em blocos casualizados com quatro repetições. Concluiu-se que: - o crescimento das plantas é inicialmente lento, intensificando-se posteriormente, com maiores incrementos entre 30 e 45 dias; - no final do ciclo, a planta acumulou 905,88 g de matéria seca, assim distribuída; caule e ramos 19,38%, folhas 30,32% e flores e frutos 50,30%; - a concentração dos nutrientes na parte aérea variou com a idade e o órgão considerado; - verifica-se nos frutos uma tendência de redução nos teores dos macronutrientes (com exceção do enxofre); - a parte aérea acumulou, aos 75 dias, em miligramas por planta: 23,08 de N; 3,46 de P; 28,90 de K; 12,74 de Ca; 5,55 de Mg; 1,59 de S. - são exportados numa colheita de uma tonelada de frutos, cerca de 1,78 kg de N; 0,33 kg de P; 2,65 kg de K; 0,14 kg de Ca; 0,21 kg de Mg; 0,09 kg de S.
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Visando estabelecer a marcha de absorção, quantidade exportada e reciclada de nitrogênio, fósforo, potássio e cálcio na bananeira cultivar Prata, instalou-se um ensaio no Estado do Espírito Santo, em janeiro de 1980, em terreno com declividade média de 40% e solo cambissólico distrófico. Sorteou-se três plantas matrizes do plantio até 465 dias após (colheita),sendo bimensal até 300 dias e mensal até o final, totalizando doze estádios de desenvolvimento, e três plantas rebento, mensalmente, da emissão até 300 dias após, atingindo nove estádios. Cada bananeira foi dividida em folha, pecíolo, pseudocaule, rizoma, engaço, botão floral e fruto para se analisar os macronutrientes. A partir dos dados coletados ajustou-se um programa de regressão e obteve-se as curvas de absorção de macronutrientes, cujos resultados permitiram concluir que, as plantas matriz e rebento: necessitam de expressivas quantidades de macronutrientes para desenvolver e produzir; apresentam uma absorção de macronutrientes que acompanha a matéria seca acumulada pelos órgãos, exceto para o potássio no período; absorvem mais de setenta e cinco por cento de nitrogênio, fósforo e potássio após o estádio de 180 dias e acima de setenta por cento do cálcio e magnésio, a partir de 240 e 120 dias; podem repor ao solo grande parte dos macronutrientes absorvidos; apresentam uma razão de absorção de macronutrientes de 20 K:12 N:10 Mg: 9 Ca: 1 P e de 26,5:8 N:4,5 Mg: 4,5 Ca: 1 P. Conclui-se ainda que: deve-se considerar a quantidade de macronutrientes exportados com a colheita e consumo dos frutos, principalmente nitrogênio, fósforo e potássio, em um programa de adubação; a planta rebento acumula mais macronutrientes do que a planta matriz.
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Investigou-se a transferência de marcadores genéticos e a presença de DNA plasmidial em 240 culturas de Escherichia coli originárias de água de esgoto (afluente e fluentes) da Estação de Tratamento da Ilha do Governador, na cidade do Rio de Janeiro, RJ. Experimentos de conjugação com E. coli K 12 permitiram o isolamento de transconjugantes com resistência a antibióticos (Su, Sm, Tc, Cm e Ap); a metais pesados (Cu, Hg e Zn) e fatores colicinogênicos (Col Ia, Ib e V) principalmente para os coliformes isolados nos setores terminais da estação de tratamento. A distribuição de plasmídeos foi prevalente nas culturas de E. coli advindas dos efluentes, com percentuais superiores a 65.
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This paper presents practical experiences using Open educational Resources (OER) for basic and elementary education (K12), educational research and research training on two inter-institutional projects with the collaboration of thirteen higher education institutions and with the support of the Corporación de Universidades para el Desarrollo del Internet (CUDI) and by the Consejo Nacional de Ciencia y Tecnología (CONACYT) of Mexico and hosted by the Tecnológico de Monterrey. The first initiative is titled "Knowledge Hub for K-12 Education" with the main goal of enrich a catalog of Open Educational Resources for basic and elementary education (K-12) for Mexico and Spanish speaking countries in Latin-America. The main goal of the second initiative is to build a collection of Open Educational Resources for Mobile Learning to address the issue of educational research and research training.
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The interest in crambe (Crambe abyssinica ) cultivation in Brazil is on the rise, whereas information on the nutrient requirements for this crop is scarce. The objective of this work was to evaluate the effect of nitrogen-phosphorus-potassium (N-P2O5-K2O formula 8:28:16) fertilization (0, 150, and 300 kg ha-1) on crambe shoot biomass production, grain and oil yields, and nutrient extraction and exportation in the second growing season after soybean. The experiment with a Haplorthox (Dystroferric Red Latosol) was carried out for two years in Botucatu, São Paulo State, Brazil. A randomized complete block design with eight replications was used. Fertilization with NPK at sowing increased the shoot biomass production, grain yield, grain oil content, as well as nutrient extraction and exportation at harvest. In the fertilized treatments, the average amounts of nutrients extracted per hectare were 91 kg K, 71 kg N, 52 kg Ca, 9.4 kg P, 9.4 kg Mg, 7.9 kg S, 2,348 g Fe, 289 g Zn, 135 g Mn, and 18.2 g Cu; while the average values of nutrient exportation per hectare were 54 kg N, 20 kg K, 12.3 kg Ca, 10 kg P, 6.6 kg S, 3.2 kg Mg, 365 g Zn, 60 g Fe, 50 g Mn, and 7.3 g Cu, with NPK fertilizer application.
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The Iowa Communications Network (ICN) is the country’s premier distance learning and state government Network, committed to providing Iowans with convenient, equal access to education, government and healthcare. The Network makes it possible for Iowans, physically separated by location, to interact in an efficient, creative, and cost-effective manner. ICN provides high-speed Internet, data, video conferencing, and voice (phone) services to authorized users, under Code of Iowa, which includes: K-12 schools, higher education, hospitals, state and federal government, National Guard armories, and libraries.
Resumo:
In recent years in both Iowa and nationally, there has been a strong focus on increasing salary levels for teachers. Iowa initiated the Student Achievement and Teacher Quality Program in fiscal year 2002 to improve the quality of K-12 teachers in Iowa schools. The program's funding to improve teacher salaries continues and is now part of the school-aid formula. During the same time period, there has been a growing focus on recruiting and improving the quality of school district superintendents and other school administrators. This issue review looks at the growth of salaries over the past ten years for Iowa's full-time teachers, superintendents and other administrators.
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It has been shown that for the reaction catalyzed by "biodegradative" L-threonine dehydratase from E. coli strains K-12 and 980 in 0.5 M phosphate-carbonate buffer, pH 8.4 and pH 9.5, the plots of initial reaction rate (v) versus the initial substrate concentration ([S]0 are characterized by several inflection points, i. e. an intermediate plateau. The plot of v versus the allosteric activator (AMP) concentration have very complicated shapes: there are several inflection points, and also the maximum at L-threonine concentration equal to 3-10(2) and 5-10(-2) M. High AMP concentrations inhibit the enzyme at high substrate concentrations. The reduced glutathion dose not influence the enzyme and does not alter the activating effect of AMP. On the basis of the data obtained it is proposed that the substrate and AMP shift the equilibrium between multiple oligomeric enzyme forms differing in catalytic activity and kinetic manifestations of allosteric interactions between the active and allosteric AMP-binding sites towards polymerization. Thus, the functioning the enzyme under study is discussed in the frames of the model of dissociating regulatory enzymes with multiple intermediate oligomeric forms.
Resumo:
Com o objetivo de avaliar o efeito da omissão de macronutrientes no crescimento, nos sintomas de deficiências nutricionais e na composição mineral em gravioleiras, conduziu-se experimento em casa de vegetação, mediante a técnica do elemento faltante. O delineamento foi o inteiramente casualizado, com quatro repetições e sete tratamentos, sendo completo (N, P, K, Ca, Mg, S e micronutrientes) e com omissão individual de N, P, K, Ca, Mg e S. As omissões dos macronutrientes afetaram o crescimento em altura, diâmetro das plantas e produção de matéria seca, quando comparados ao tratamento completo. Com base nos teores em g/kg dos macronutrientes nas folhas, encontraram-se os seguintes valores adequados nos tratamento completo e com omissão: N = 14,70 e 8,82; P = 0,92 e 0,47; K = 12,35 e 2,62; Ca = 14,11 e 3,44; Mg = 3,59 e 1,09; S = 5,32 e 2,30.
Resumo:
Minna Canth, eg. Ulrika Wilhelmina Canth, f. Johnsson f. 19.3.1844 i Tammerfors d. 12.5.1897 i Kuopio Minna Canth har uppfattats som en av de viktigaste författarna inom 1880-talets finländska realism. I sin produktion behandlade hon många av sin tids samhälleliga missförhållanden, så som arbetarklassens fattigdom och kvinnornas svaga ställning i samhället. Canths drama Työmiehen vaimo (1885, sv. Arbetarens hustru, översatt till svenska 1886) markerar inledningen på en samhällskritisk period i författarskapet. Skådespelet Kovan onnen lapsia (1888, sv. Olyckans barn, tidigare kallad De vanlottade) väckte upprörda känslor och ströks ur repertoaren vid Finska teatern efter den första föreställningen. Sedermera har man lyft fram kombinationen av samhälleliga och psykologiska tendenser i Canths produktion. Canth var även verksam som tidningsskribent och skrev många polemiska artiklar om kvinnans ställning. Hon deltog också aktivt i den litterära diskussionen och organiserade litterära salonger för tidens framväxande bildade klass, den s.k. ”Minnan salonki” (sv. Minnas salong) i sin hemgård Kanttila. Biografiskt lexikon för Finland: http://www.blf.fi/artikel.php?id=2816
Resumo:
Zachris Topelius s. 14.1.1818 Kuddnäsissä, Uusikaarlepyyn lähellä k. 12.3.1898 Sipoossa Zachris Topelius on yksi aikansa suurimpia suomalaisia kirjailijoita, kulttuuripersoonia ja yhteiskunnallisia vaikuttajia. Topeliuksen pääosin 1800-luvun jälkipuoliskolla syntyneessä tuotannossa vallitsee kansallisromantiikan henki, mutta teoksista löytyy myös realistisia piirteitä. Ominaista Topeliuksen kansankuvaukselle on naiivi tunteellisuus ja isänmaallisuus. Kirjailijan teoksiin vaikutti myös hänen kristillinen elämänkatsomuksensa. Topeliuksen mittavan tuotannon tärkeimpiin teoksiin kuuluvat lapsille suunnatut teokset, esimerkiksi Lukemisia lapsille (Läsning för barn I-VIII, 1865‒1896) sekä oppikirja Maamme kirja (Boken om vårt land, 1875), jotka vaikuttivat ajan ruotsinkielisen lastenkirjallisuuden uudistumiseen. Suomessa Topelius oli edelläkävijä myös historiallisten romaanien ja novellien kirjoittajana. Hänen teoksiaan ovat esimerkiksi Suomen herttuatar (Hertiginnan af Finland, 1850) ja Välskärin kertomuksia (Fältskärns berättelser I-V, kirjana 1853‒1867). Topelius tunnettiin lisäksi tuotteliaana lyyrikkona, jonka useita runoja on sävelletty. Virsi- ja laulutuotantoon kuuluvat sellaiset edelleen tunnetut virret ja laulut kuten ”En etsi valtaa loistoa” (”Julvisa”/”Gif mig ej glans”, 1887), ”Kesäpäivä Kangasalla” (”En sommardag i Kangasala”, 1853), ”Sylvian joululaulu” (”Sylvias hälsning från Sicilien”/”Sylvias julvisa”, 1853) ja ”Pajulaulu” (”Videvisan”/”Sof, du lilla vide ung”, 1869). - - - - - Zachris Topelius f. 14.1.1818 i Kuddnäs, nära Nykarleby d. 12.3.1898 i Sibbo Zachris Topelius är en av de största finländska författarna, kulturpersonligheterna och samhällspåverkarna under sin tid. Hans litterära produktion, främst tillkommen under den senare halvan av 1800-talet, präglas av den nationella romantikens anda men även av realistiska drag. Topelius fosterländskhet och bild av det finländska karakteriseras av en naiv känslosamhet och är starkt präglad av hans kristna livssyn. Till det mest betydelsefulla i hans omfattande författarskap hör hans produktion för barn (t.ex. Läsning för barn I-VIII, 1865-1896 samt läroboken Boken om vårt land, 1875) som innebar en förnyelse av tidens svenskspråkiga barnlitteratur. Han var också en föregångare som finländsk författare till historiska romaner och noveller (t.ex. Hertiginnan af Finland, 1850 och Fältskärns berättelser I-V, i bokform 1853-1867), samt mycket produktiv som lyriker. Topelius lyrik har i flera fall tonsatts och han är upphovsman till en rad klassiska och fortfarande sjungna psalmer och andra sånger (t.ex. Julvisa/Gif mig ej glans…, 1887, En sommardag i Kangasala, 1853, Sylvias hälsning från Sicilien/Sylvias julvisa, 1853 och Videvisan/Sof, du lilla vide ung, 1869). Kansallisbiografia: http://www.kansallisbiografia.fi/kb/artikkeli/2854/ http://www.blf.fi/artikel.php?id=2854
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Escherichia coli K-12 (pEGFPluxABCDEAmp) (E. coli-lux), constitutively emitting bioluminescence (BL), was constructed and its BL emitting properties tested in different growth and killing conditions. The BL emission directly correlated with the number of viable E. coli-lux cells, and when subjected to the antimicrobial agent, the diminishment of the BL signal was linked directly to the number of killed bacterial cells. The method provided a very convenient application, especially when compared to conventional plate counting assays. This novel real-time based method was utilized in both immunological and toxicological assessments. The parameters such as the activation phase, the lytic phase and the capacity of the killing of the serum complement system were specified not only in humans but also in other species. E. coli-lux was also successfully used to study the antimicrobial activities of insect haemolymph. The mechanisms of neutrophil activity, like that of a myeloperoxidase (MPO)-H2O2-halide system, were studied using the E. coli-lux approach. The fundamental role of MPO was challenged, since during the actual killing in described circumstances in phagolysosome the MPO system was inactivated and chlorination halted. The toxicological test system, assessing indoor air total toxicity, particularly suitable for suspected mold damages, was designed based on the E. coli-lux method. Susceptibility to the vast number of various toxins, both pure chemicals and dust samples from the buildings and extracts from molds, were investigated. The E. coli-lux application was found to possess high sensitivity and specificity attributes. Alongside the analysis system, the sampling kit for indoor dust was engineered based on the swipe stick and the container. The combination of practical specimen collector and convenient analysis system provided accurate toxic data from the dust sample within hours. Neutrophils are good indicators of the pathophysiological state of the individual, and they can be utilized as a toxicological probe due to their ability to emit chemiluminescence (CL). Neutrophils can either be used as probe cells, directly exposed to the agent studied, or they can act as indicators of the whole biological system exposed to the agent. Human neutrophils were exposed to the same toxins as tested with the E. coli-lux system and measured as luminol amplified CL emission. The influence of the toxins on the individuals was investigated by exposing rats with moniliniformin, the mycotoxin commonly present in Finnish grains. The activity of the rat neutrophils was found to decrease significantly during the 28 days of exposure.
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Salmonella enterica sérovar Typhi (S. Typhi) est l’agent responsable de la fièvre typhoïde et cause environ 200 000 morts et 27 millions de cas annuellement. C’est un pathogène entérique dont le réservoir est restreint à l’Homme. Les raisons de cette restriction d’hôte sont méconnues et pourraient dépendre de l’expression de facteurs d’adhésion à des étapes importantes au cours de la pathogenèse. L’annotation bioinformatique du génome de S. Typhi identifie 12 fimbriae de type chaperon-placier (FCP), un curli ainsi qu’un pilus de type IV. L’objectif de ce projet de recherche est d’étudier ces systèmes d’adhésion peu caractérisés. D’abord, le niveau d’expression de ces gènes a été évalué dans différentes conditions de culture in vitro en utilisant une approche de gènes rapporteurs. L’expression des 14 systèmes d’adhésion a été détectée. Nos résultats indiquent qu’une carence en fer favorise l’expression des opérons bcf et csg. Indépendamment du fer, l’expression de bcf, csg, pil, sef, sta, stc, stg et sth est influencée par la richesse nutritive du milieu. L’incubation en milieu LB liquide favorise l’expression de la plupart des systèmes d’adhésion par rapport à un milieu LB liquide sans agitation ou un milieu LB solide. En somme, l’expression des systèmes d’adhésion de S. Typhi a été observée et est influencée par des conditions environnementales. Dans un second volet, nous avons tent de surexprimer les différents systèmes d’adhésion chez une souche d’E. coli ou de S. Typhi afimbriaire. Avec cette approche, nous avons été en mesure de démontrer que l’opéron tcf encode pour un fimbria fonctionnel que l’on a pu observer en microscopie électronique. L’expression de tcf chez une souche afimbriaire d’E. coli et S. Typhi a également diminué leur capacité d’adhésion à des cellules épithéliales intestinales humaines lors d’essais in vitro. Nos observations démontrent que l’expression des systèmes d’adhésion retrouvés chez S. Typhi est influencée par les conditions enviroi9onnementales. Au moins un de ces systèmes est fonctionnel. Ceci suggère une contribution des systèmes d’adhésion retrouvés chez S. Typhi lors de l’interaction de ce pathogène avec l’humain.
Characterization and Pathogenicity of Vibrio cholerae and Vibrio vulnificus from Marine environments
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The genus Vibrioof the family Vibrionaceae are Gram negative, oxidasepositive, rod- or curved- rodshaped facultative anaerobes, widespread in marine and estuarine environments. Vibrio species are opportunistic human pathogens responsible for diarrhoeal disease, gastroenteritis, septicaemia and wound infections and are also pathogens of aquatic organisms, causing infections to crustaceans, bivalves and fishes. In the present study, marine environmental samples like seafood and water and sediment samples from aquafarms and mangroves were screened for the presence of Vibrio species. Of the134 isolates obtained from the various samples, 45 were segregated to the genus Vibrio on the basis of phenotypic characterization.like Gram staining, oxidase test, MoF test and salinity tolerance. Partial 16S rDNA sequence analysis was utilized for species level identification of the isolates and the strains were identified as V. cholerae(N=21), V. vulnificus(N=18), V. parahaemolyticus(N=3), V. alginolyticus (N=2) and V. azureus (N=1). The genetic relatedness and variations among the 45 Vibrio isolates were elucidated based on 16S rDNA sequences. Phenotypic characterization of the isolates was based on their response to 12 biochemical tests namely Voges-Proskauers’s (VP test), arginine dihydrolase , tolerance to 3% NaCl test, ONPG test that detects β-galactosidase activity, and tests for utilization of citrate, ornithine, mannitol, arabinose, sucrose, glucose, salicin and cellobiose. The isolates exhibited diverse biochemical patterns, some specific for the species and others indicative of their environmental source.Antibiogram for the isolates was determined subsequent to testing their susceptibility to 12 antibiotics by the disc diffusion method. Varying degrees of resistance to gentamycin (2.22%), ampicillin(62.22%), nalidixic acid (4.44%), vancomycin (86.66), cefixime (17.77%), rifampicin (20%), tetracycline (42.22%) and chloramphenicol (2.22%) was exhibited. All the isolates were susceptible to streptomycin, co-trimoxazole, trimethoprim and azithromycin. Isolates from all the three marine environments exhibited multiple antibiotic resistance, with high MAR index value. The molecular typing methods such as ERIC PCR and BOX PCR revealed intraspecies relatedness and genetic heterogeneity within the environmental isolatesof V. cholerae and V. vulnificus. The 21 strains of V. choleraewere serogroupedas non O1/ non O139 by screening for the presence O1rfb and O139 rfb marker genes by PCR. The virulence/virulence associated genes namely ctxA, ctxB, ace, VPI, hlyA, ompU, rtxA, toxR, zot, nagst, tcpA, nin and nanwere screened in V. cholerae and V. vulnificusstrains.The V. vulnificusstrains were also screened for three species specific genes viz., cps, vvhand viu. In V. cholerae strains, the virulence associated genes like VPI, hlyA, rtxA, ompU and toxR were confirmed by PCR. All the isolates, except for strain BTOS6, harbored at least one or a combination of the tested genes and V. choleraestrain BTPR5 isolated from prawn hosted the highest number of virulence associated genes. Among the V. vulnificusstrains, only 3 virulence genes, VPI, toxR and cps, were confirmed out of the 16 tested and only 7 of the isolates had these genes in one or more combinations. Strain BTPS6 from aquafarm and strain BTVE4 from mangrove samples yielded positive amplification for the three genes. The toxRgene from 9 strains of V. choleraeand 3 strains of V. vulnificus were cloned and sequenced for phylogenetic analysis based on nucleotide and the amino acid sequences. Multiple sequence alignment of the nucleotide sequences and amino acid sequences of the environmental strains of V. choleraerevealed that the toxRgene in the environmental strains are 100% homologous to themselves and to the V. choleraetoxR gene sequence available in the Genbank database. The 3 strains of V. vulnificus displayed high nucleotide and amino acid sequence similarity among themselves and to the sequences of V. cholerae and V. harveyi obtained from the GenBank database, but exhibited only 72% homology to the sequences of its close relative V. vulnificus. Structure prediction of the ToxR protein of Vibrio cholerae strain BTMA5 was by PHYRE2 software. The deduced amino acid sequence showed maximum resemblance with the structure of DNA-binding domain of response regulator2 from Escherichia coli k-12 Template based homology modelling in PHYRE2 successfully modelled the predicted protein and its secondary structure based on protein data bank (PDB) template c3zq7A. The pathogenicity studies were performed using the nematode Caenorhabditiselegansas a model system. The assessment of pathogenicity of environmental strain of V. choleraewas conducted with E. coli strain OP50 as the food source in control plates, environmental V. cholerae strain BTOS6, negative for all tested virulence genes, to check for the suitability of Vibrio sp. as a food source for the nematode;V. cholerae Co 366 ElTor, a clinical pathogenic strain and V. cholerae strain BTPR5 from seafood (Prawn) and positive for the tested virulence genes like VPI, hlyA, ompU,rtxA and toxR. It was found that V. cholerae strain BTOS6 could serve as a food source in place of E. coli strain OP50 but behavioral aberrations like sluggish movement and lawn avoidance and morphological abnormalities like pharyngeal and intestinal distensions and bagging were exhibited by the worms fed on V. cholerae Co 366 ElTor strain and environmental BTPR5 indicating their pathogenicity to the nematode. Assessment of pathogenicity of the environmental strains of V. vulnificus was performed with V. vulnificus strain BTPS6 which tested positive for 3 virulence genes, namely, cps, toxRand VPI, and V. vulnificus strain BTMM7 that did not possess any of the tested virulence genes. A reduction was observed in the life span of worms fed on environmental strain of V. vulnificusBTMM7 rather than on the ordinary laboratory food source, E. coli OP50. Behavioral abnormalities like sluggish movement, lawn avoidance and bagging were also observed in the worms fed with strain BTPS6, but the pharynx and the intestine were intact. The presence of multi drug resistant environmental Vibrio strainsthat constitute a major reservoir of diverse virulence genes are to be dealt with caution as they play a decisive role in pathogenicity and horizontal gene transfer in the marine environments.
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Esta investigación buscó indagar sobre la pasantía como un momento particular de la transición educativo-laboral de un individuo; para ello, planteé la pasantía como un momento intermedio en el que el estudiante vive cambios estructurales de la etapa laboral, aún en la etapa educativa. El análisis se centra en el proceso vivido por estudiantes universitarios durante su pasantía laboral en un escenario de trabajo real en diálogo con su formación profesional. Los resultados de esta investigación están orientados a responder a la pregunta ¿Cómo viven los estudiantes universitarios de un programa de sociología la transición educativo-laboral por medio de las pasantías laborales, en relación con su contexto y con el de la pasantía? El análisis se sustenta en una reflexión de carácter etnográfico que tuvo como punto de partida mí experiencia como pasante, narrada en un diario de campo y su contraste con seis casos de estudiantes de sociología de la Universidad del Rosario que pasaron por dicha experiencia; la aproximación a estos casos se dio a través de una serie de entrevistas a profundidad. Así mismo, desarrollé observaciones participantes en los Seminarios de seguimiento de las pasantías del programa de Sociología en la Universidad del Rosario, lo que me permitió elegir los casos que hicieron parte de la investigación. Por otro lado, elaboré un contexto de la profesión en Colombia desde 1959 con énfasis en la implementación de las pasantías a través de una revisión de archivo, acompañada de entrevistas a coordinadores de pasantía de diferentes universidades de Bogotá para conocer el momento actual de las pasantías; lo anterior, con el fin de dar un contexto más amplio de éste momento particular de la transición educativo-laboral.