Development of impedimetric and optical calcium biosensor by using modified gold electrode with porcine S100A12 protein

We describe the development of a label free method to analyze the interactions between Ca(2+) and the porcine S100A12 protein immobilized on polyvinyl butyral (PVB). The modified gold electrodes were characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and surface plasmon resonance (SPR) techniques. SEM analyses of PVB and PVB-S100A12 showed a heterogeneous distribution of PVB spherules on gold surface. EIS and CV measurements have shown that redox probe reactions on the modified gold electrodes were partially blocked due the adsorption of PVB-S100A12, and confirm the existence of a positive response of the immobilized S100Al2 to the presence of calcium ions. The biosensor exhibited a wide linear response to Ca(2+) concentrations ranging from 12.5 to 200 mM. The PVB-S100A12 seems to be bound to the gold electrode surface by physical adsorption: we observed an increase of 1184.32 m degrees in the SPR angle after the adsorption of the protein on the PVB surface (in an indication that 9.84 ng of S100A12 are adsorbed per mm(2) of the Au-PVB electrode), followed by a further increase of 581.66 m degrees after attachment of the Ca(2+) ions. In addition, no SPR response is obtained for non-specific ions. These studies might be useful as a platform for the design of new reusable and sensitive biosensing devices that could find use in the clinical applications. (C) 2010 Elsevier B.V. All rights reserved.

Fyra lärare om könsskillnader och framgång i skolan : Varför ges flickor i högre grad än pojkar ett högre kursbetygjämfört med nationellt provbetyg i engelska 5 och engelska 6?

Flickor har under de senaste decennierna presterat bättre än pojkar i den svenska skolan. Attflickornas studieresultat har förbättrats skall naturligtvis ses som en framgång; samtidigt måstelikvärdigheten och jämställdheten i skolan värnas och pojkarnas sämre resultat tas på allvar. Härhar de nationella proven i skolan betydelse eftersom huvudsyftet med dessa är att just stödja enlikvärdig bedömning och betygssättning i den svenska skolan. Dock konstaterar Skolverket attflickor i högre utsträckning än pojkar får ett högre kursbetyg jämfört med det betyg de fick på detnationella provet i samma kurs. För ämnet engelska i gymnasiet så gäller även samma negativaförhållande för pojkarna när det gäller avvikelse som ger ett lägre kursbetyg än provbetyg, det villsäga pojkar får i högre utsträckning än flickor ett lägre kursbetyg jämfört med provbetyget församma kurs.Syftet med uppsatsen är att svara på frågeställningarna (1) Vilka orsaker anger mina respondentertill att det slutliga kursbetyget kan avvika från provbetyget? samt (2) Hur beskriverrespondenterna att dessa avvikelser är kopplade till elevernas kön?För att besvara ovan frågor har en mindre kvalitativ studie genomförts där fyra engelskalärare pågymnasiet har intervjuats. I bakgrunden redogörs för hur flickors och pojkars studieprestationsett ut historiskt, hur bedömningen av ämnet engelska sker samt skillnader i betygssättningmellan nationellt prov och kurs gällande engelska 5 och engelska 6. Det teoretiska perspektivetgrundar sig på tidigare forskning kring könsskillnader relaterat till studieprestation med fokus påflickors och pojkars beteenden samt attityder. Teorin är uppdelat i områdena ansträngning,motivation, förväntningar, självförtroende och stress.Resultatet visar att de intervjuade lärarna upplever att skillnaderna i flickors och pojkarsstudieprestationer är i linje med vad forskning gällande det teoretiska perspektivet visat. Dessakönsskillnader, kopplat till att nationellt prov och kurs mäter olika förmågor och även betygssättsefter olika riktlinjer, är tillsammans möjliga påverkansfaktorer till att flickor i högre utsträckningän pojkar får ett högre kursbetyg jämfört med det betyg de skrev på det nationella provet.

Tratamento da leishmaniose cutanea difusa (LCD) com a associacao antimoniato-N-metilglucamina (Glucantime®) e sulfato de paramomicina (Gabbrox®)

Diffuse cutaneous leishmaniasis (DCL) is a rare form of tegumentary leishmaniasis (TL) and 350 cases have been reported world wide. Presently in Brazil, there were 31 cases reported corresponding to 8.9% of the world cases. The state of Maranhao contributed with ten cases (32.3%) of national reports. Recently the authors had opportunity to follow two new cases of DCL, both from the countryside of that state totaling 12 cases. The parasite Leishmania L. amazonensis was incriminated in the disease, confirming the available data as being the unique species to produce DCL in this state. The therapy associating meglumine anthimoniate with paramomicine sulphate (Gabbrox®) during 90 days, showed good tolerance and favorable response, being another option for fills form of DCL that constituted a challenge to science so far.

El capital social campesino en la gestión del desarrollo rural: díadas, equipos, puentes y escaleras

Incluye Bibliografía

A gestão de resíduos sólidos em contextos intraorganizacionais: um estudo a partir da UFPA

Esta dissertação centra esforços em responder à seguinte pergunta: Como se deu o processo de implantação do Programa Coleta Seletiva Solidária na Universidade Federal do Pará? O referido programa foi instituído pelo Decreto Federal nº 5.940 do dia 25 de outubro de 2006 e visa à destinação do material reciclável produzido nos órgãos públicos federais da administração direta e indireta às associações e cooperativas de catadores. Esta ação, apesar de restrições quanto a sua destinação, vem contribuir com a diminuição da pobreza e o impacto ambiental em áreas naturais. O objetivo desta pesquisa é conhecer e analisar o processo de implantação do Programa Coleta Seletiva Solidária na UFPA. Teve o apoio das bases teóricas do Neoinstitucionalismo em Hall e Taylor e comparações das abordagens teóricas de Olson e Ostrom sobre a Teoria Comportamental da Ação Coletiva na produção de um Bem Público. A metodologia utilizada foi de caráter descritivo e qualitativo. A teoria conduziu ao trabalho de campo para as técnicas de observação, entrevistas semiestruturadas e pesquisa documental. Foram realizadas 25 entrevistas com técnicos da Prefeitura da Cidade Universitária da UFPA, diretores e coordenadores de Unidades Acadêmicas além de funcionários dos serviços gerais. Como resultado, foi constatada a necessidade de um trabalho de interação dentro das próprias Unidades Acadêmica, destas entre si e, paralelamente, com a Administração Superior. Em Ostrom, encontrou-se o apoio ao direcionamento teórico em razão de sua concepção de uso comum e as decisões coletivas de cooperação baseadas na reciprocidade e confiança entre as pessoas. Ela acrescenta elementos à Teoria da Ação Coletiva de Olson e mostra que trabalhos em busca do bem comum são possíveis desde que haja regras nas quais todos estão sujeitos aos benefícios das externalidades advindas. Com as abordagens institucionais e de ação coletiva apresentadas, este estudo pretende contribuir com a aplicação da Gestão Ambiental na Administração Pública. Os resultados alcançados podem levar à prática da Coleta Seletiva Solidária da UFPA, incentivando os gestores do interior do Estado a engajarem-se ao programa.

Considering sustainability factors in the development project life-cycle : a framework for increasing successful adoption of improved stoves

Indoor air pollution from combustion of solid fuels is the fifth leading contributor to disease burden in low-income countries. This, and potential to reduce environmental impacts, has resulted in emphasis on use of improved stoves. However, many efforts have failed to meet expectations and effective coverage remains limited. A disconnect exists between technologies, delivery methods, and long-term adoption. The purpose of this research is to develop a framework to increase long-term success of improved stove projects. The framework integrates sustainability factors into the project life-cycle. It is represented as a matrix and checklist which encourages consideration of social, economic, and environmental issues in projects. A case study was conducted in which an improved stove project in Honduras was evaluated using the framework. Results indicated areas of strength and weakness in project execution and highlighted potential improvements for future projects. The framework is also useful as a guide during project planning.

Pseudomonas chlororaphis subsp. piscium subsp. nov., isolated from freshwater fish

Gram-negative, aerobic, motile, rod-shaped bacteria were isolated from the intestines of freshwater fish on two separate occasions. Colonies of both strains, JF3835(T) and JF4413, produced non-diffusible green pigment following 4-5 days incubation on Luria-Bertani agar. The most abundant fatty acids were summed feature 3 (comprising C(16 : 1)ω7c and/or C(15 : 0) iso 2-OH), C(16 : 0) and C(18 : 1)ω7c. The DNA G+C content was 62.9 mol%. Sequence analysis of the 16S rRNA gene indicated 100 % sequence similarity between the two strains. In comparison with recognized species, the new strains exhibited the greatest degree of sequence similarity with members of the Pseudomonas chlororaphis subspecies: P. chlororaphis subsp. chlororaphis (99.84 %), P. chlororaphis subsp. aurantiaca (99.75 %) and P. chlororaphis subsp. aureofaciens (99.40 %). While DNA-DNA relatedness confirmed the placement of strains JF3835(T) and JF4413 as members of the species P. chlororaphis, multilocus sequencing indicated that the strains formed a distinct cluster within it. On the basis of genotypic and phenotypic evidence, strains JF3835(T) and JF4413 represent a novel subspecies of the species P. chlororaphis, for which the name Pseudomonas chlororaphis subsp. piscium subsp. nov. is proposed. The type strain is JF3835(T) (=NCIMB 14478(T)=DSM 21509(T)).

A mutation in hairless dogs implicates FOXI3 in ectodermal development

Mexican and Peruvian hairless dogs and Chinese crested dogs are characterized by missing hair and teeth, a phenotype termed canine ectodermal dysplasia (CED). CED is inherited as a monogenic autosomal semidominant trait. With genomewide association analysis we mapped the CED mutation to a 102-kilo-base pair interval on chromosome 17. The associated interval contains a previously uncharacterized member of the forkhead box transcription factor family (FOXI3), which is specifically expressed in developing hair and teeth. Mutation analysis revealed a frameshift mutation within the FOXI3 coding sequence in hairless dogs. Thus, we have identified FOXI3 as a regulator of ectodermal development.

La infancia en las prácticas y en el discurso

Este trabajo desarrolla una visión de conjunto sobre la relación entre fenómenos histórico-sociales, ideologías y legislación acerca de la infancia. Describe el ámbito institucional, sus características y modalidades de asistencia y la evolución operada en las prácticas de protección a la infancia poniendo énfasis en la jurisdicción de Córdoba que ofrece marcadas diferencias tanto ideológico-culturales, cuanto respecto de su participación e influencia en el desarrollo histórico y jurídico del país. Realiza un análisis del discurso institucional y del académico jurídico posterior a la sanción del Código Civil, a través de tesis doctorales y otras fuentes históricas dando cuenta de las perspectivas que desde el campo intelectual y académico desarrollaron la lógica de intervención estatal sobre la infancia, hasta comienzos del siglo XX, cuando se sancionan leyes específicas de protección a nivel nacional y se formaliza la intervención del Estado mediante instituciones asistenciales y jurídicas que enmarcaron la protección a la infancia durante todo el siglo pasado

La infancia en las prácticas y en el discurso

Este trabajo desarrolla una visión de conjunto sobre la relación entre fenómenos histórico-sociales, ideologías y legislación acerca de la infancia. Describe el ámbito institucional, sus características y modalidades de asistencia y la evolución operada en las prácticas de protección a la infancia poniendo énfasis en la jurisdicción de Córdoba que ofrece marcadas diferencias tanto ideológico-culturales, cuanto respecto de su participación e influencia en el desarrollo histórico y jurídico del país. Realiza un análisis del discurso institucional y del académico jurídico posterior a la sanción del Código Civil, a través de tesis doctorales y otras fuentes históricas dando cuenta de las perspectivas que desde el campo intelectual y académico desarrollaron la lógica de intervención estatal sobre la infancia, hasta comienzos del siglo XX, cuando se sancionan leyes específicas de protección a nivel nacional y se formaliza la intervención del Estado mediante instituciones asistenciales y jurídicas que enmarcaron la protección a la infancia durante todo el siglo pasado