Disruption of amylase genes by RNA interference affects reproduction in the Pacific oyster Crassostrea gigas

Feeding strategies and digestive capacities can have important implications for variation in energetic pathways associated with ecological and economically important traits, such as growth or reproduction in bivalve species. Here, we investigated the role of amylase in the digestive processes of Crassostrea gigas, using in vivo RNA interference. This approach also allowed us to investigate the relationship between energy intake by feeding and gametogenesis in oysters. Double-stranded (ds)RNA designed to target the two α-amylase genes A and B was injected in vivo into the visceral mass of oysters at two doses. These treatments caused significant reductions in mean mRNA levels of the amylase genes: −50.7% and −59% mRNA A, and −71.9% and −70.6% mRNA B in 15 and 75 µg dsRNA-injected oysters, respectively, relative to controls. Interestingly, reproductive knock-down phenotypes were observed for both sexes at 48 days post-injection, with a significant reduction of the gonad area (−22.5% relative to controls) and germ cell under-proliferation revealed by histology. In response to the higher dose of dsRNA, we also observed reductions in amylase activity (−53%) and absorption efficiency (−5%). Based on these data, dynamic energy budget modeling showed that the limitation of energy intake by feeding that was induced by injection of amylase dsRNA was insufficient to affect gonadic development at the level observed in the present study. This finding suggests that other driving mechanisms, such as endogenous hormonal modulation, might significantly change energy allocation to reproduction, and increase the maintenance rate in oysters in response to dsRNA injection.

Kids, raccoons and roos : awkward encounters and mixed affects

Suomen turvallisuuspoliittisen ratkaisun lähtökohtia

Suomi luopui puolueettomuuspolitiikasta turvallisuuspoliittisena linjanaan kun se liittyi Euroopan unioniin ennen kaikkea turvallisuussyistä. Vaikka Suomi on EU-jäsenyyden myötä poliittisesti liittoutunut ja vaikka se on jatkuvasti laajentanut ja syventänyt yhteistyötään NATOn kanssa, Suomi on yhä sotilaallisesti liittoutumaton valtio. Suomen turvallisuuspoliittinen toimintaympäristö on ollut poikkeuksellisen vakaa kylmän sodan jälkeen ennen kaikkea siksi, että maailman voimakkain suurvalta Yhdysvallat on yhä sotilaallisesti läsnä Euroopassa ja Euroopan integraatio on jatkunut ja syventynyt. Suomi on sitonut nykyisen turvallisuuspoliittisen linjansa turvallisuusympäristönsä pysyvyyteen. Viime vuosina NATOn laajentuminen entisen Neuvostoliiton alueelle on voimistanut läntisten suurvaltojen ja Venäjän välistä valtakamppailua ja turvallisuuskilpailua Suomen lähialueella. Viimeistään Ukrainan konflikti on tehnyt ajankohtaiseksi myös kysymyksen Suomen sotilaallisesta liittoutumisesta. Tässä artikkeliteoksessa turvallisuuspolitiikan asiantuntijat analysoivat keskeisimpien ulkoisten toimijoiden – EU:n, Ruotsin, Viron, Venäjän, Yhdysvaltojen ja NATOn – merkitystä Suomen turvallisuuspoliittisen toimintaympäristön vakaudelle ja Suomen turvallisuudelle sekä pohtivat, onko Suomen syytä muuttaa nykyistä turvallisuuspoliittista linjaansa turvallisuusympäristössä tapahtuneiden muutosten vuoksi. Kirjoittajat myös arvioivat Suomen mahdolliseen sotilaalliseen liittoutumiseen liittyviä etuja ja haittoja verrattuna nykyiseen turvallisuuspoliittiseen ratkaisuun. Teoksen kirjoittajien toisistaan poikkeavat näkemykset Suomen turvallisuuspoliittisen toimintaympäristön vakaudesta, NATOn laajentumisen vaikutuksista Itämeren alueella, Venäjän valtapolitiikasta ja sen Suomelle muodostaman sotilaallisen uhan voimakkuudesta, NATOn pelotteen uskottavuudesta ja Yhdysvaltojen sitoutumisesta liittolaistensa puolustamiseen sekä Suomen NATO-jäsenyyden eduista ja haitoista valaisevat laajasti kysymystä Suomen turvallisuudesta ja turvallisuuspoliittisesta ratkaisusta. Suomelle sen geopoliittinen asema Venäjälle strategisesti elintärkeiden Kuolan alueen ja Pietari–Moskova-ydinalueen välittömässä läheisyydessä on ongelmallinen maan turvallisuuden kannalta. Suurvalta Venäjä onkin edelleen keskeisin Suomen turvallisuuteen ja turvallisuuspoliittiseen ratkaisuun vaikuttava ulkoinen toimija. Neuvostoliiton hajoamisen jälkeen Suomella on ollut ainutlaatuinen mahdollisuus liittoutua Yhdysvaltojen kanssa NATO-jäsenyyden myötä Venäjän potentiaalista sotilaallista uhkaa vastaan. Toisaalta Suomella ei kuitenkaan ole ollut pakottavaa tarvetta siihen, koska se on välillisesti hyötynyt Yhdysvaltojen suurstrategiasta Euroopassa ja koska Venäjän valtapolitiikka on kohdistunut ennen kaikkea entisen Neuvostoliiton alueelle. Vaikka Ukrainan konflikti onkin lisännyt poliittista ja sotilaallista jännitettä Itämeren alueella, tämän teoksen perusteella on kuitenkin mahdotonta antaa yksiselitteistä vastausta siihen, pitäisikö Suomen sen turvallisuuspoliittisessa toimintaympäristössä tapahtuneen muutoksen seurauksena liittyä NATOn jäseneksi vai ei. Halutessaan Suomi voi siis edelleen jatkaa nykyisellä turvallisuuspoliittisella linjallaan sotilaallisesti liittoutumattomana valtiona, mutta samalla sen täytyy kuitenkin pitää myös sotilaallinen liittoutuminen avoimena turvallisuuspoliittisena vaihtoehtona tulevaisuudessa.

Interaction between mercury (Hg), arsenic (As) and selenium (Se) affects the activity of glutathione S-transferase in breast milk: possible relationship with fish and shellfish intake

Breast milk is regarded as an ideal source of nutrients for the growth and development of neonates, but it can also be a potential source of pollutants. Mothers can be exposed to different contaminants as a result of their lifestyle and environmental pollution. Mercury (Hg) and arsenic (As) could adversely affect the development of fetal and neonatal nervous system. Some fish and shellfish are rich in selenium (Se), an essential trace element that forms part of several enzymes related to the detoxification process, including glutathione S-transferase (GST). The goal of this study was to determine the interaction between Hg, As and Se and analyze its effect on the activity of GST in breast milk. Milk samples were collected from women between day 7 and 10 postpartum. The GST activity was determined spectrophotometrically; total Hg, As and Se concentrations were measured by atomic absorption spectrometry. To explain the possible association of Hg, As and Se concentrations with GST activity in breast milk, generalized linear models were constructed. The model explained 44% of the GST activity measured in breast milk. The GLM suggests that GST activity was positively correlated with Hg, As and Se concentrations. The activity of the enzyme was also explained by the frequency of consumption of marine fish and shellfish in the diet of the breastfeeding women.

Evaluation of Adoptive T Cell Therapy and Oncolytic Virotherapy for Treatment of Brain Tumors

Adoptive immunotherapy and oncolytic virotherapy are two promising strategies for treating primary and metastatic malignant brain tumors. We demonstrate the ability of adoptively transferred tumor-specific T cells to rapidly mediate the clearance of established brain tumors in several mouse models. Similar to the clinical situation, tumor recurrences are frequent and result from immune editing of tumors. T cells can eliminate antigen-expressing tumor cells but are not effective against antigen loss variant (ALV) cancer cells that multiply and repopulate a tumor. We show that the level of tumor antigen present affects the success of adoptive T cell therapy. When high levels of antigen are present, tumor stromal cells such as microglia and macrophages present tumor peptide on their surface. As a result, T cells directly eliminate cancer cells and cross-presenting stromal cells and indirectly eliminate ALV cells. We were able to show the first direct evidence of tumor antigen cross-presentation by CD11b+ stromal cells in the brain using soluble, high-affinity T cell receptor monomers. Strategies that target brain tumor stroma or increase antigen shedding from tumor cells leading to increased crosspresentation by stromal cells may improve the clinical success of T cell adoptive therapies. We evaluated one potential strategy to complement adoptive T cell therapy by characterizing the oncolytic effects of myxoma virus (MYXV) in a syngeneic mouse brain tumor model of metastatic melanoma. MYXV is a rabbit poxvirus with strict species tropism for European rabbits. MYXV can also infect mouse and human cancer cell lines due to signaling defects in innate antiviral mechanisms and hyperphosphorylation of Akt. MYXV kills B16.SIY melanoma cells in vitro, and intratumoral injection of virus leads to robust, selective and transient infection of the tumor. We observed that virus treatment recruits innate immune cells iii to the tumor, induces TNFα and IFNβ production in the brain, and results in limited oncolytic effects in vivo. To overcome this, we evaluated the safety and efficacy of co-administering 2C T cells, MYXV, and neutralizing antibodies against IFNβ. Mice that received the triple combination therapy survived significantly longer with no apparent side effects, but eventually relapsed. Based on these findings, methods to enhance viral replication in the tumor and limit immune clearance of the virus will be pursued. We conclude that myxoma virus should be further explored as a vector for transient delivery of therapeutic genes to a tumor to enhance T cell responses.

In vitro expansion of U87-MG human glioblastoma cells under hypoxic conditions affects glucose metabolism and subsequent in vivo growth

International audience

The timing and interval of mate encounter affects investment during mating

The benefits obtained from mating are usually condition-dependent, favouring the evolution of flexible investment during copulation, for instance, in terms of invested time, energy, or sperm. Flexible investment strategies are predicted to depend on the likelihood of acquiring alternative mates and therefore they should depend on the timing of mate encounter. However, scarce experimental evidence for this hypothesis exists. Here we manipulated the time delay until first mating and the interval between first and second mating in the polygynandrous common lizard, Zootoca vivipara. We determined treatment effects on fertilisation success and copulation duration, the latter being a proxy for investment in mating and for quantity of transferred sperm. The duration of the second copulation decreased with increasing inter-mating interval and depended on the fertilisation success of first mates. The former provides evidence for time-dependent investment strategies, most likely resulting from the progression of the female's reproductive cycle. Fertilisation success of first mates increased with increasing inter-mating interval and was higher when females were closer to ovulation, showing that flexible investment strategies significantly affected male reproductive success. This points to fertilisation assurance, which may mitigate negative effects of low population density on reproductive success, e.g. Allee effects.

Glucose-dependent insulinotropic polypeptide (GIP) directly affects collagen fibril diameter and collagen cross-linking in osteoblast cultures.

International audience

Medial and lateral hamstrings and quadriceps co-activation affects knee joint kinematics and ACL elongation : a pilot study

Chronic exposure to sub-lethal concentration of a glyphosate-based herbicide alters hormone profiles and affects reproduction of female Jundia (Rhamdia quelen)

This work was carried out to verify the effect of a glyphosate-based herbicide on Jundia hormones (cortisol, 17 beta-estradiol and testosterone), oocyte and swim-up fry production. Earthen ponds containing Jundia females were contaminated with glyphosate (3.6 mg/L); blood samples were collected from eight females from each treatment immediately before, or at 1, 10, 20 30 and 40 days following contamination. A typical post-stress rise in cortisol levels was observed at the 20th and 40th days following exposure to glyphosate. At the 40th day, 17 beta-estradiol was decreased in the exposed females. A similar number of oocytes were stripped out from females from both groups, however, a lower number of viable swim-up fry were obtained from the herbicide exposed females, which also had a higher liver-somatic index (LSI). The results indicate that the presence of glyphosate in water was deleterious to Rhamdia quelen reproduction, altering steroid profiles and egg viability. (c) 2006 Elsevier B.V. All rights reserved.

Fluency and attitudes

The goal of this article is to review how, when, and why f luency, or processing ease, affects attitudes. The current article first defines f luency and then discusses its direct impact on attitudes, noting that f luency usually makes attitudes more positive and that it does so for a wide array of attitude objects. Mechanisms and moderators of these direct effects are also described. The article then summarizes how f luency can affect attitudes indirectly, through its impact on other judgments (like perceptions of confidence or truth) and on cognitive operations (like information processing). The article ends by highlighting a few areas where additional research is likely to reap impressive benefits.

Sugarcane trash levels in soil affects the fungi but not bacteria in a short-term field experiment.

The sugarcane in Brazil is passing through a management transition that is leading to the abolition of pre-harvest burning. Without burning, large amounts of sugarcane trash is generated, and there is a discussion regarding the utilization of this biomass in the industry versus keeping it in the field to improve soil quality. To study the effects of the trash removal on soil quality, we established an experimental sugarcane plantation with different levels of trash over the soil (0%, 50% and 100% of the original trash deposition) and analyzed the structure of the bacterial and fungal community as the bioindicators of impacts. The soil DNA was extracted, and the microbial community was screened by denaturing gradient gel electrophoresis in two different seasons. Our results suggest that there are no effects from the different levels of trash on the soil chemistry and soil bacterial community. However, the fungal community was significantly impacted, and after twelve months, the community presented different structures among the treatments.

Iron content affects lipogenic gene expression in the muscle of nelore beef cattle.

Iron (Fe) is an essential mineral for metabolism and plays a central role in a range of biochemical processes. Therefore, this study aimed to identify differentially expressed (DE) genes and metabolic pathways in Longissimus dorsi (LD) muscle from cattle with divergent iron content, as well as to investigate the likely role of these DE genes in biological processes underlying beef quality parameters. Samples for RNA extraction for sequencing and iron, copper, manganese, and zinc determination were collected from LD muscles at slaughter. Eight Nelore steers, with extreme genomic estimated breeding values for iron content (Fe-GEBV), were selected from a reference population of 373 animals. From the 49 annotated DE genes (FDR<0.05) found between the two groups, 18 were upregulated and 31 down-regulated for the animals in the low Fe-GEBV group. The functional enrichment analyses identified several biological processes, such as lipid transport and metabolism, and cell growth. Lipid metabolism was the main pathway observed in the analysis of metabolic and canonical signaling pathways for the genes identified as DE, including the genes FASN, FABP4, and THRSP, which are functional candidates for beef quality, suggesting reduced lipogenic activities with lower iron content. Our results indicate metabolic pathways that are partially influenced by iron, contributing to a better understanding of its participation in skeletal muscle physiology.

Exploring first-year academic achievement through structural equation modelling

The purpose of this research was to develop and test a multicausal model of the individual characteristics associated with academic success in first-year Australian university students. This model comprised the constructs of: previous academic performance, achievement motivation, self-regulatory learning strategies, and personality traits, with end-of-semester grades the dependent variable of interest. The study involved the distribution of a questionnaire, which assessed motivation, self-regulatory learning strategies and personality traits, to 1193 students at the start of their first year at university. Students' academic records were accessed at the end of their first year of study to ascertain their first and second semester grades. This study established that previous high academic performance, use of self-regulatory learning strategies, and being introverted and agreeable, were indicators of academic success in the first semester of university study. Achievement motivation and the personality trait of conscientiousness were indirectly related to first semester grades, through the influence they had on the students' use of self-regulatory learning strategies. First semester grades were predictive of second semester grades. This research provides valuable information for both educators and students about the factors intrinsic to the individual that are associated with successful performance in the first year at university.