Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT)

The NF-κB family member p65 is central to inflammation and immunity. The purpose of this study was to identify and characterize evolutionary conserved genes modulating p65 transcriptional activity. Using an RNAi screening approach, we identified chaperonin containing TCP1 subunit η (CCTη) as a regulator of Drosophila NF-κB proteins, Dorsal and Dorsal-related immunity factor (Dif). CCTη was also found to regulate NF-κB-driven transcription in mammalian cells, acting in a promoter-specific context, downstream of IκB kinase (IKK). CCTη knockdown repressed IκBα and CXCL2/MIP2 transcription during the early phase of NF-κB activation while impairing the termination of CCL5/RANTES and CXCL10/IP10 transcription. The latter effect was associated with increased DNA binding and reduced p65 acetylation, presumably by altering the activity of histone acetyltransferase CREB-binding protein (CBP). We identified p65 lysines (K) 122 and 123 as target residues mediating the CCTη-driven termination of NF-κB-dependent transcription. We propose that CCTη regulates NF-κB activity in a manner that resolves inflammation.

Ages analysis, trace elements and relative frequencies of foraminifera snd diatoms from sediment cores AvH248260-2 and AvH248260-1, Ameralik Fjord, SW Greenland

A multiproxy record including benthic foraminifera, diatoms and XRF data of a marine sediment core from a SW Greenland fjord provides a detailed reconstruction of the oceanographic and climatic variations of the region during the last 4400 cal. years. The lower part of our record represents the final termination of the Holocene Thermal Maximum. After the onset of the 'Neoglaciation' at approximately 3.2 ka cal. BP, the fjord system was subject to a number of marked hydrographical changes that were closely linked to the general climatic and oceanographic development of the Labrador Sea and the North Atlantic region. Our data show that increased advection of Atlantic water (Irminger Sea Water) from the West Greenland Current into the Labrador Sea was a typical feature of Northeast Atlantic cooling episodes such as the 'Little Ice Age' and the 'European Dark Ages', while the advection of Irminger Sea Water decreased significantly during warm episodes such as the 'Mediaeval Warm Period' and the 'Roman Warm Period'.Whereas the 'Mediaeval Warm Period' was characterized by relatively cool climate as suggested by low meltwater production, the preceding 'Dark Ages' display higher meltwater runoff and consequently warmer climate. When compared with European climate, these regional climate anomalies indicate persisting patterns of advection of colder, respectively warmer air masses in the study region during these periods and thus a long-term seesaw climate pattern between West Greenland and Europe.

Economic impact report for proposed regulations, coil coating segment of machinery and mechanical products point source category /

"December 10, 1980."

Basic experimental studies on boiling fluid flow and heat transfer at elevated pressures for month of ...

"Monthly progress report".

[Colonial records of Pennsylvania]

Collection of documents, supplemented by the companion series "Pennsylvania archives". (See preliminary reports of the committees, and of the editors, S. Hazard, and the Act providing for the publication, 1837, in Pennsylvania archives, v.1, p. 1-23, especially p. 7 and 17-23, where the present collection is officially designated as the "Colonial records", a title not used in printing, the vols. having special titles only, as given in "Contents" below)

Superconductive properties of thin tin films,

"This work was supported in part by the General Research Provision of contract between the Air Force and Space Technology Laboratories, Inc., and in part by the Office of Naval Research."

Current induced switching of superconductive thin films,

"This work was supported in part by the General Research Provision of contract between the Air Force and Space Technology Laboratories, Inc., and in part by the Office of Naval Research."

High temperature machining methods. Phase I: Evaluation of elevated temperature machining methods.

"Submitted in partial fulfillment of Contract A. F.---33(600)40066."

Origines celticae (a fragment) and other contributions to the history of Britain,

Prefatory notice of the author: p. vii-xxviii.

Flight evaluation of various phugoid dynamics and 1/Th values for the landing-approach task.

"This is the second in a series of two reports dealing with longitudinal handling qualities in the landing approach." (see also TL/711/L3/C43+)

The effect of entrainment upon a jet-flapped airfoil.

"Submitted to the Office of Scientific Research of the Air Research and Development Command, United States Air Force, in partial fulfillment of contract number AF 18(600)-1523."

A digest of English civil law,

v.1. General, by Edward Jenks.--v.2. (part 1) Law of contract (general) by R. W. Lee.--v.2. (part 2) Law of contract (particular contracts) by R. W. Lee.--v.2. (part 3) Law of quasi-contract and tort, by J. C. Miles.--v.3. Law of property, by Edward Jenks.--v.4. Family law, by W. M. Geldart. Succession, by W. S. Holdsworth.--v.5. Succession (cont'd.) by W. S. Holdsworth.

Methods for changing consumer attitudes: a report of three experiments. Prepared for Quartermaster Food & Container Institute for the Armed Forces

At head of title: PRA report 61-2, Feb. 1961.

A Cultural resource survey of the proposed St. Louis Harbor site locations, Madison County, Illinois /

Submitted to the United States Army Corps of Engineers, St. Louis District, in partial fulfillment of Contract #DACW43-81-M-2728.

An overview of the archaeological resources of the metropolitan St. Louis area /

A report submitted to the National Park Service by the Illinois State Museum Society in fulfillment of contract no. CX0001-4-0093.