Different true-protein sources do not modify the metabolism of crossbred Bos taurus x Bos indicus growing heifers

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Glycerol combined with oils did not limit biohydrogenation of unsaturated fatty acid but reduced methane production in vitro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Degradabilidade ruminal da matéria seca de folhas e colmo de genótipos de Cynodon spp. em quatro idades de rebrota

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach

Background: Forestomach fermentation in Australian marsupials such as wallabies and kangaroos, though analogous to rumen fermentation, results in lower methane emissions. Insights into hydrogenotrophy in these systems could help in devising strategies to reduce ruminal methanogenesis. Reductive acetogenesis may be a significant hydrogen sink in these systems and previous molecular analyses have revealed a novel diversity of putative acetogens in the tammar wallaby forestomach.Results: Methanogen-inhibited enrichment cultures prepared from tammar wallaby forestomach contents consumed hydrogen and produced primarily acetate. Functional gene (formyltetrahydrofolate synthetase and acetyl-CoA synthase) analyses revealed a restricted diversity of Clostridiales species as the putative acetogens in the cultures. A new acetogen (growth on H-2/CO2 with acetate as primary end product) designated isolate TWA4, was obtained from the cultures. Isolate TWA4 classified within the Lachnospiraceae and demonstrated > 97% rrs identity to previously isolated kangaroo acetogens. Isolate TWA4 was a potent hydrogenotroph and demonstrated excellent mixotrophic growth (concomitant consumption of hydrogen during heterotrophic growth) with glycerol. Mixotrophic growth of isolate TWA4 on glycerol resulted in increased cell densities and acetate production compared to autotrophic growth. Co-cultures with an autotrophic methanogen Methanobrevibacter smithii revealed that isolate TWA4 performed reductive acetogenesis under high hydrogen concentration (> 5 mM), but not at low concentrations. Under heterotrophic growth conditions, isolate TWA4 did not significantly stimulate methanogenesis in a co-culture with M. smithii contrary to the expectation for organisms growing fermentatively.Conclusions: The unique properties of tammar wallaby acetogens might be contributing factors to reduced methanogen numbers and methane emissions from tammar wallaby forestomach fermentation, compared to ruminal fermentation. The macropod forestomach may be a useful source of acetogens for future strategies to reduce methane emissions from ruminants, particularly if these strategies also include some level of methane suppression and/or acetogen stimulation, for example by harnessing mixotrophic growth capabilities

Metabolism and ruminal parameters of Holstein × Gir heifers fed sugarcane and increasing levels of crude protein

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The effects of Diet and corticosteroid-induced immune suppression during infection by Haemonchus contortus in lambs

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Utilização de pastagem de alfafa e raspa de mandioca na produção de leite de cabras

Pós-graduação em Zootecnia - FMVZ

Desenvolvimento do rúmen de bovinos no período pré-natal

The development of the domestic mammals’ stomach is a complex process, especially in ruminants. The objective of this research was to perform the analysis of structures of this organ during prenatal period. Samples of Nelore fetuses rumen (Bos Taurus indicus) were divided into five groups: 1 - fetuses with 9 to 15 weeks (8 to 21cm) of gestation, 2 - fetuses with 16 to 22 weeks (23 to 37cm), 3 - fetuses at 23 to 29 weeks (40 to 58cm) 4 - fetuses with 30 to 36 weeks (61 to 77cm) and 5 - fetuses with 37 to 43 weeks (79 to 88cm). Sections were stained with Hematoxylin and eosin and Mallory's trichrome and examined at light microscope. In group 1, in fetuses with 11 cm, could be seen all the layers, mucosa with high epithelium and light cells, lamina propria fused with submucosa, the muscular layer was with two thin sublayers similar in thickness and serosa was thin too. With 13.5 cm, was observed the first irregularities in the mucosa with epithelium projections and lamina propria that will lead rumen papillae. With 16.5 cm there was differentiation of the lamina propria to the submucosa. In group 2, in fetuses with 28 cm were observed complete papillae. From this stage, the remaining groups, there was an increase in thickness of the epithelium, lamina propria, submucosal and muscular layer whit the inner sublayer more thicker than the external and serosa. It was concluded that the main variations occur in fetuses of groups 1 and 2, and after this phase, there is increased in thickness of all layers until the end of the pregnancy period.

A standartized research protocol for platelet-rich plasma (PRP) preparation in rats

Introduction: The urgent need for studies using standardized protocols to evaluate the real biological effects of PRP has been emphasized by several authors. Objective: The purpose of this study was to standardize a methodology for autologous Platelet-Rich Plasma (PRP) preparation in rats. Material and methods: Twentyfour, 5 to 6-month-old, male rats, weighing 450 to 500 g were used. After general anesthesia, 3.15 ml of blood was collected from each animal, via cannulation of the jugular vein. A standardized technique of double centrifugation was used to prepare PRP. PRP samples and peripheral blood platelets were then manually counted using a Neubauer chamber. Student’s t-test was used to compare the differences between the number of platelets in peripheral blood and PRP samples (p < 0.05). In addition, PRP and peripheral blood smears were stained to see platelets’ morphology. Results: All surgical procedures were well tolerated by the animals and they were healthy during the entire experimental period. PRP samples showed higher significantly platelet concentrations than peripheral blood samples (2,677,583 and 683,680 respectively). Conclusion: Within the limits of this study, it can be concluded that the method used produced autologous PRP with appropriated platelet quantity and quality, in rats.

Períodos de adaptação de bovinos Nelore confinados a dietas de alto teor de concentrado

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Óleo funcional na dieta de vacas leiteiras

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Silagens de milho consorciado com forrageira tropical e leguminosa em dietas para terminação de cordeiros

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Acidose ruminal subaguda em ovinos Santa Inês: estudo clínico, laboratorial e avaliação da laminite por termografia infravermelha e radiologia digital

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fluxo de gases de efeito estufa na implantação de sistema silvipastoril em pastejo com ovinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Protocolos experimentais de confinamento para bovinos Nelore alimentados com dietas contendo glicerina bruta

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)