Profile of Washington & Fayette streets, Georgetown, D.C. /

Washington Street profile covers 30th Street between Q and R streets N.W. Fayette Street profile covers 35th Street between Volta Place and Wisconsin Avenue N.W.

Part of "Widows Mite" lying in the co. of Washington D.C. ; The above 2 plats represent two separate parts of that portion of a tract of land called Josephs Park, lying in Montg. Co. Md. /

Both tracts border on Rock Creek.

DnaG interacts with a linker region that joins the N- and C-domains of DnaB and induces the formation of 3-fold symmetric rings

Loading of the replicative ring helicase onto the origin of replication (oriC) is the final outcome of a well coordinated series of events that collectively constitute a primosomal cascade. Once the ring helicase is loaded, it recruits the primase and signals the switch to the polymerization mode. The transient nature of the helicase-primase (DnaB-DnaG) interaction in the Escherichia coli system has hindered our efforts to elucidate its structure and function. Taking advantage of the stable DnaB-DnaG complex in Bacillus stearothermophilus, we have reviewed conflicting mutagenic data from other bacterial systems and shown that DnaG interacts with the flexible linker that connects the N- and C-terminal domains of DnaB. Furthermore, atomic force microscopy (AFM) imaging experiments show that binding of the primase to the helicase induces predominantly a 3-fold symmetric morphology to the hexameric ring. Overall, three DnaG molecules appear to interact with the hexameric ring helicase but a small number of complexes with two and even one DnaG molecule bound to DnaB were also detected. The structural/functional significance of these data is discussed and a speculative structural model for this complex is suggested.

An��lisis de las sanciones internacionales en el marco del tratado de no proliferaci��n nuclear. Estudio de caso: energ��a at��mica en Ir��n (2006-2015)

El presente trabajo de Grado tiene como prop��sito examinar la incidencia de las sancionesinternacionales en el marco del r��gimen de no proliferaci��n nuclear en el caso de Ir��n durante el periodo 2006-2015, teniendo en cuenta factores hist��ricos de a��os anteriores. Se analiza y explica c��mo las sanciones internacionales pueden ser una medida persuasiva por violar ciertos art��culos del Tratado de no Proliferaci��n Nuclear. Finalmente identifica y analiza los tipos de sanciones econ��micas, financieras y comerciales que los Estados y el Consejo de Seguridad de las Naciones Unidas le han impuesto a Ir��n, as�� como la manera en que estas han incidido en la esfera pol��tica iran�� y mundial.

Argumentos duales: el principio de dualidad como una estructura argumentativa

El objetivo de la tesis es identificar una familia de argumentos que comparten una estructura con el principio de dualidad de la geometr��a proyectiva. Esta familia la denomino "argumentos duales". Para lograr este objetivo, tomo cuatro argumentos importantes de la filosof��a anal��tica e identifico en ellos la estructura que comparten. Los cuatro argumentos son: (i) el acertijo de la inducci��n de Goodman; (ii) la indeterminaci��n de la referencia Putnam; (iii) la indeterminaci��n de la traducci��n de Quine; (iv) la paradoja del seguimiento de reglas de Wittgenstein.

La construcci��n de sentido (���sensemaking���) de un entorno estrat��gico turbulento por parte de los altos directivos: un estudio en una multinacional automotriz

Este art��culo presenta los resultados de una investigaci��n realizada al interior de dos contextos. Por un lado, el te��rico, en el marco de uno de los discursos m��s relevantes en los campos de la estrategia organizacional, de la managerial and organizational cognition (MOC) y, en general, de los estudios organizacionales (organization studies): la construcci��n de sentido (sensemaking). Por el otro, el emp��rico, en una de las grandes compa����as multinacionales del sector automotriz con presencia global. Esta corporaci��n enfrenta una permanente tensi��n entre lo que dicta la casa matriz, en relaci��n con el cumplimiento de metas y est��ndares espec��ficos, considerando el mundo entero, y los retos que, teniendo en cuenta lo regional y lo local, experimentan los altos directivos encargados de hacer prosperar la empresa en estos lugares. La aproximaci��n implementada fue cualitativa. Esto en atenci��n a la naturaleza de la problem��tica abordada y la tradici��n del campo. Los resultados permiten ampliar el actual nivel de comprensi��n acerca de los procesos de sensemaking de los altos directivos al enfrentar un entorno estrat��gico turbulento.

A IMPLANTA����O FERROVI��RIA NO ESTADO DO RIO DE JANEIRO: 1854-1898

Esse artigo �� parte da pesquisa que pretende analisar a import��ncia da ferrovia para a circula����o na regi��o Norte Fluminense do Estado do Rio de Janeiro. A pesquisa, ainda n��o conclu��da, est�� baseada nos preceitos da Geografia Hist��rica (ver SILVA, 2008). Com isso �� necess��rio analisar o processo maior de implanta����o ferrovi��ria no Estado do Rio de Janeiro desde o in��cio da implanta����o ferrovi��ria, em 1854, da primeira ferrovia brasileira, a Imperial Companhia de Navega����o a Vapor e Estrada de Ferro de Petr��polis. A partir da�� surge a Ferrovia Dom Pedro II, uma das mais importantes do pa��s e depois a Estrada de Ferro Cantagalo. S��o ent��o implantadas diversas ferrovias, sempre atendendo �� interesses regionais capitaneados por lideran��as locais. Assim �� que a ferrovia chega em Campos dos Goytacazes em 1875. Outra importante ferrovia do estado foi a Estrada de Ferro Leopoldina, que foi incorporando v��rias pequenas linhas f��rreas, at�� a transforma����o desta ferrovia em outra empresa a ���The Leopoldina Railway Company���, que come��a a funcionar em 1898, substituindo o caos reinante na companhia anterior. O conhecimento do per��odo inicial de configura����o de uma rede ferrovi��ria no Norte Fluminense passa a ser de fundamental import��ncia devido �� import��ncia da Companhia Leopoldina para a regi��o Norte Fluminense em per��odos posteriores.

Aspectos pol��ticos para el estudio del crecimiento de la poblaci��n urbana en Costa Rica en los per��odos intercensales 1950-63 y 1963 - 1973

Apicaci��n del m��todo estad��stico para analizar las tasas de crecimiento demogr��fico de la poblaci��n de 74 centros urbanos cantonales de Costa Rica

Frequency Of The Allelic Variant C.1150t > C In Exon 10 Of The Fibroblast Growth Factor Receptor 3 (fgfr3) Gene Is Not Increased In Patients With Pathogenic Mutations And Related Chondrodysplasia Phenotypes.

Mutations in the FGFR3 gene cause the phenotypic spectrum of FGFR3 chondrodysplasias ranging from lethal forms to the milder phenotype seen in hypochondroplasia (Hch). The p.N540K mutation in the FGFR3 gene occurs in ���70% of individuals with Hch, and nearly 30% of individuals with the Hch phenotype have no mutations in the FGFR3, which suggests genetic heterogeneity. The identification of a severe case of Hch associated with the typical mutation c.1620C > A and the occurrence of a c.1150T > C change that resulted in a p.F384L in exon 10, together with the suspicion that this second change could be a modulator of the phenotype, prompted us to investigate this hypothesis in a cohort of patients. An analysis of 48 patients with FGFR3 chondrodysplasia phenotypes and 330 healthy (control) individuals revealed no significant difference in the frequency of the C allele at the c.1150 position (p = 0.34). One patient carrying the combination `pathogenic mutation plus the allelic variant c.1150T > C' had a typical achondroplasia (Ach) phenotype. In addition, three other patients with atypical phenotypes showed no association with the allelic variant. Together, these results do not support the hypothesis of a modulatory role for the c.1150T > C change in the FGFR3 gene.

Urothelial Carcinogen Resistance Driven By Stronger Toll-like Receptor 2 (tlr2) And Uroplakin Iii (up Iii) Defense Mechanisms: A New Model.

The objective of the study was to illustrate the applicability and significance of the novel Lewis urothelial cancer model compared to the classic Fisher 344. Fischer 344 and Lewis females rats, 7��weeks old, were intravesical instilled N-methyl-N-nitrosourea 1.5��mg/kg every other week for a total of four doses. After 15��weeks, animals were sacrificed and bladders analyzed: histopathology (tumor grade and stage), immunohistochemistry (apoptotic and proliferative indices) and blotting (Toll-like receptor 2-TLR2, Uroplakin III-UP III and C-Myc). Control groups received placebo. There were macroscopic neoplastic lesions in 20��% of Lewis strain and 70��% of Fischer 344 strain. Lewis showed hyperplasia in 50��% of animals, normal bladders in 50��%. All Fischer 344 had lesions, 20��% papillary hyperplasia, 30��% dysplasia, 40��% neoplasia and 10��% squamous metaplasia. Proliferative and apoptotic indices were significantly lower in the Lewis strain (p��<��0.01). The TLR2 and UP III protein levels were significantly higher in Lewis compared to Fischer 344 strain (70.8 and 46.5��% vs. 49.5 and 16.9��%, respectively). In contrast, C-Myc protein levels were significantly higher in Fischer 344 (22.5��%) compared to Lewis strain (13.7��%). The innovative Lewis carcinogen resistance urothelial model represents a new strategy for translational research. Preservation of TLR2 and UP III defense mechanisms might drive diverse urothelial phenotypes during carcinogenesis in differently susceptible individuals.

Post Hoc Analysis Of The Patricia Randomized Trial Of The Efficacy Of Human Papillomavirus Type 16 (hpv-16)/hpv-18 As04-adjuvanted Vaccine Against Incident And Persistent Infection With Nonvaccine Oncogenic Hpv Types Using An Alternative Multiplex Type-specific Pcr Assay For Hpv Dna.

The efficacy of the human papillomavirus type 16 (HPV-16)/HPV-18 AS04-adjuvanted vaccine against cervical infections with HPV in the Papilloma Trial against Cancer in Young Adults (PATRICIA) was evaluated using a combination of the broad-spectrum L1-based SPF10 PCR-DNA enzyme immunoassay (DEIA)/line probe assay (LiPA25) system with type-specific PCRs for HPV-16 and -18. Broad-spectrum PCR assays may underestimate the presence of HPV genotypes present at relatively low concentrations in multiple infections, due to competition between genotypes. Therefore, samples were retrospectively reanalyzed using a testing algorithm incorporating the SPF10 PCR-DEIA/LiPA25 plus a novel E6-based multiplex type-specific PCR and reverse hybridization assay (MPTS12 RHA), which permits detection of a panel of nine oncogenic HPV genotypes (types 16, 18, 31, 33, 35, 45, 52, 58, and 59). For the vaccine against HPV types 16 and 18, there was no major impact on estimates of vaccine efficacy (VE) for incident or 6-month or 12-month persistent infections when the MPTS12 RHA was included in the testing algorithm versus estimates with the protocol-specified algorithm. However, the alternative testing algorithm showed greater sensitivity than the protocol-specified algorithm for detection of some nonvaccine oncogenic HPV types. More cases were gained in the control group than in the vaccine group, leading to higher point estimates of VE for 6-month and 12-month persistent infections for the nonvaccine oncogenic types included in the MPTS12 RHA assay (types 31, 33, 35, 45, 52, 58, and 59). This post hoc analysis indicates that the per-protocol testing algorithm used in PATRICIA underestimated the VE against some nonvaccine oncogenic HPV types and that the choice of the HPV DNA testing methodology is important for the evaluation of VE in clinical trials. (This study has been registered at ClinicalTrials.gov under registration no. NCT00122681.).

Long-term Decline Of The Amazon Carbon Sink.

Atmospheric carbon dioxide records indicate that the land surface has acted as a strong global carbon sink over recent decades, with a substantial fraction of this sink probably located in the tropics, particularly in the Amazon. Nevertheless, it is unclear how the terrestrial carbon sink will evolve as climate and atmospheric composition continue to change. Here we analyse the historical evolution of the biomass dynamics of the Amazon rainforest over three decades using a distributed network of 321 plots. While this analysis confirms that Amazon forests have acted as a long-term net biomass sink, we find a long-term decreasing trend of carbon accumulation. Rates of net increase in above-ground biomass declined by one-third during the past decade compared to the 1990s. This is a consequence of growth rate increases levelling off recently, while biomass mortality persistently increased throughout, leading to a shortening of carbon residence times. Potential drivers for the mortality increase include greater climate variability, and feedbacks of faster growth on mortality, resulting in shortened tree longevity. The observed decline of the Amazon sink diverges markedly from the recent increase in terrestrial carbon uptake at the global scale, and is contrary to expectations based on models.

The C-terminal Region Of The Human P23 Chaperone Modulates Its Structure And Function.

The p23 protein is a chaperone widely involved in protein homeostasis, well known as an Hsp90 co-chaperone since it also controls the Hsp90 chaperone cycle. Human p23 includes a ��-sheet domain, responsible for interacting with Hsp90; and a charged C-terminal region whose function is not clear, but seems to be natively unfolded. p23 can undergo caspase-dependent proteolytic cleavage to form p19 (p231-142), which is involved in apoptosis, while p23 has anti-apoptotic activity. To better elucidate the function of the human p23 C-terminal region, we studied comparatively the full-length human p23 and three C-terminal truncation mutants: p23���������������; p23��������������� and p23���������������. Our data indicate that p23 and p19 have distinct characteristics, whereas the other two truncations behave similarly, with some differences to p23 and p19. We found that part of the C-terminal region can fold in an ��-helix conformation and slightly contributes to p23 thermal-stability, suggesting that the C-terminal interacts with the ��-sheet domain. As a whole, our results suggest that the C-terminal region of p23 is critical for its structure-function relationship. A mechanism where the human p23 C-terminal region behaves as an activation/inhibition module for different p23 activities is proposed.

Avalia����o do teor e da estabilidade de vitaminas do complexo B e vitamina C em bebidas isot��nicas e energ��ticas

Vitamin C stability and concentration was evaluated in isotonic beverages and B group vitamins (B1, B2, B3, B5 and B6) in power beverages. The amount of vitamins was found to be above of that declared on the labels, even after the shelf life had been exceeded. A small decrease in the amount of B group vitamins was observed during the shelf life of the products. In the case of vitamin C this decrease was slightly higher. The present research shows the need of increased quality control and inspection.

Alila����o e crotila����o catal��tica e enantiosseletiva de alde��dos

The field of chiral catalysis has experienced explosive growth over the last two decades. By now, many of the classical reactions in organic synthesis can be carried out efficiently in asymmetric manner. As one of the fundamental and powerful C-C bond-forming reactions, enantioselective catalytic allylation (ECA) and crotylation (ECC) of aldehydes has attracted considerable attention. In this article, we present an overview about the importance of chiral Lewis acids and bases in catalytic enantioselective addition of allyl- and crotyl metals to aldehydes and the application of this methodology in the total synthesis of natural and non-natural products.