The role of PKCzeta in amikacin-induced apoptosis in the cochlea: prevention by aspirin.

Aminoglycoside antibiotics are ototoxic, inducing irreversible sensorineural hearing loss mediated by oxidative and excitotoxic stresses. The NF-kappaB pathway is involved in the response to aminoglycoside damage in the cochlea. However, the molecular mechanisms of this ototoxicity remain unclear. We investigated the expression of PKCzeta, a key regulator of NF-kappaB activation, in response to aminoglycoside treatment. Amikacin induced PKCzeta cleavage and nuclear translocation. These events were concomitant with chromatin condensation and paralleled the decrease in NF-kappaB (p65) levels in the nucleus. Amikacin also induced the nuclear translocation of apoptotic inducing factor (AIF). Prior treatment with aspirin prevented PKCzeta cleavage and nuclear translocation. Thus, aspirin counteracts the early effects of amikacin, thereby protecting hair cells and spiral ganglion neurons. These results demonstrate that PKCzeta acts as sentinel connecting specific survival pathways to mediate cellular responses to amikacin ototoxicity.

Sobre la publicació: "Cowroid de cerámica de cerámica vidriada hallado en el poblado ibérico del "Tossal del Moro" en Piñeras (Batea, Tarragona)"

És aquest un dels treballs pioners del Dr. Maluquer. Efectivament, feia ja un segle que s'havien començat a descobrir peces semblants a la península Iberica de les quals es tenia notícia bibliografica: un escaraboid pseudoegipci descobert a la Puerta de Tierra de Cadis (Delgado 1871: CXXXI), i un escarabeu descobert a Malaga (Rodríguez de Berlanga 1891: 328-329,332-333). Nogensmenys, abans de l' article del Dr. Maluquer que ara ens ocupa, molt pocs treballs explícitament dedicats a objectes de tipus egipci trobats a la península Iberica s'havien publicat: només podem citar-ne un dedicat a troballes d'objectes egipcis fetes a Tarragona (Castillo 1909: 169-180) i un altre dedicat al primer escarabeu egipci procedent de la necropolis d' Alcácer do Sal (Correia 1925: 90-93).

La legislació constantiniana respecte de l'episcopalis audientia.

Dues disposicions de Constantí -el Codex Theodosiallus I, 27, 1 (318) i la COllstitutio Sirmondina I (333)- constitueixen la primera san ció legal del poder jurídic deIs bisbes en afers externs a la religió. Al voltant d' elles creix una nova institució eclesiastica, l' episcopalis audientia, la qual, recollint els preceptes cristians tradicionals, aplica el dret imperial en la resolució de causes de naturales a civil. La controvertida veracitat de les lIeis constantinianes i la varietat d' interpretacions que susciten depassen l' ambit d' allo estrictament jurídic per endinsar-se en la canviant societat romana del segle IV.

A comparison of the energy budgets of two freshwater Pulmonates: Lymnaea peregra (Müller) and Physa acuta (Drap.)

The energy budgets of two freshwater gastropds, Lymnae peregra and Physa acuta, were compared in similar experimental conditions (20ºC, fed ad libitum with 24h-decayed lettuce), and found to differ in several ways. 1) L. Peregra has a higher assimilation efficiency than P. acuta (72% vs 60%). 2) These species assimilate different components of the ingested food: P. acuta uses a smaller, but more energetic part (probably mainly bacteria), whereas L. peregra assimilate a larger, but less energetic part (probably mainly cellulose). 3) L. peregra allocates more of its assimilated energy to oxygene consumption and mucus production (maintenance investments), wheras P. acuta invest more in growth and reproduction (production investments). Such differences are relevant to the natural habitat of these two species: P. acuta colonizes warm, eutrophic and temporary pools, where decaying material constitue the main part of available resources, and where adult mortality is high and impredictible. By contrast, L. peregra is frequently found in colder, oligotrophic and predictible environements, where living primary producers constitute the main part of available resources, and where biotic interactions are important factors of mortality.

[Pérouse. Basilica di San Pietro]

Référence bibliographique : Weigert, 333

Elicitation of specific cytotoxic T cells by immunization with malaria soluble synthetic polypeptides.

We have studied the immunogenicity of Plasmodium falciparum circumsporozoite (CS) protein-derived synthetic polypeptides in mice. These synthetic peptides correspond to the N- and the C-terminal domains 22-125 and 289-390, respectively of the P. falciparum 7G8 isolate CS protein expressed on the sporozoite surface. They comprise what is believed to be the mature protein, except for the central repetitive B cell domain. BALB/c (H-2d) mice were immunized s.c. with 50 micrograms soluble CS polypeptides emulsified in IFA. After a single immunization, CS-specific helper and cytotoxic T lymphocytes (CTLs) could be obtained. The resultant CTLs obtained by in vitro restimulation of primed lymph node (LN) cells recognized H-2Kd target cells in the presence of short synthetic peptides defined in the present study. These epitopes are contained within the N- and C-terminal regions of the CS protein, and correspond to sequences 39-47 and 333-342. In addition, these CTLs can specifically lyse H-2d target cells transfected with the CS gene. These results suggest that, by immunization of mice with large soluble CS synthetic polypeptides in IFA, it is possible to obtain MHC class I-restricted T cell responses specific for the CS protein. This approach might be advantageous in the formulation of efficient malaria subunit vaccines.

Longevity differs among sexes but is not affected by repeated immune activation in voles (Microtus arvalis)

Investment of resources in immune defences, despite obvious short-term benefits, may be detrimental to long-term maintenance and thus decrease longevity in absence of parasites. In addition, females and males may differ in immune investment and intrinsic longevity because they are subjected to different degrees of sexual competition and extrinsic mortality. In order to test if sex-specific investment in mounting an immune response reduced longevity, we compared the longevity of captive male and female common voles Microtus arvalis regularly challenged with keyhole limpet haemocyanin, an antigen which elicits the production of antibodies, to the longevity of voles injected with the corresponding antigen-free buffer (phosphate-buffered saline). Injections were repeated every 28 days to mimic a chronic infection. The magnitude of immune response did not vary between males and females and did not affect longevity. Overall, females lived longer than males, independently of the immune challenge. Thus, the long-term costs of immunity seem small in voles. The longevity pattern is consistent with the prediction that male-biased predation or parasitism in the wild causes reduced intrinsic lifespan, but this reduction is not mediated by a decrease in male immunity

La juventud ante la ciudadanía

En este artículo se presenta los resultados de un trabajo por encuesta realizado para el estudio de la ciudadanía con alumnado de educación secundaria obligatoria. Trata, en concreto, de la elaboración de un cuestionario para identificar los elementos que constituyen el concepto de ciudadanía del alumnado de secundaria. En primer lugar se presenta la fundamentación teórica y el modelo de ciudadanía del que se parte, en base a la cual se concretan los objetivos, el contenido, y la estructura interna del cuestionario. Tras un estudio piloto del mismo y, una vez realizadas las modificaciones oportunas, se construye el cuestionario a aplicar. El cuestionario, que aparece en el anexo del articulo, se ha aplicado a una muestra de 333 personas de seis centros de educación secundaria obligatoria con población multicultural. Finalmente, se presentan los resultados obtenidos en el estudio, así como las evidencias de las características técnicas del instrumento elaborado.

La juventud ante la ciudadanía

En este artículo se presenta los resultados de un trabajo por encuesta realizado para el estudio de la ciudadanía con alumnado de educación secundaria obligatoria. Trata, en concreto, de la elaboración de un cuestionario para identificar los elementos que constituyen el concepto de ciudadanía del alumnado de secundaria. En primer lugar se presenta la fundamentación teórica y el modelo de ciudadanía del que se parte, en base a la cual se concretan los objetivos, el contenido, y la estructura interna del cuestionario. Tras un estudio piloto del mismo y, una vez realizadas las modificaciones oportunas, se construye el cuestionario a aplicar. El cuestionario, que aparece en el anexo del articulo, se ha aplicado a una muestra de 333 personas de seis centros de educación secundaria obligatoria con población multicultural. Finalmente, se presentan los resultados obtenidos en el estudio, así como las evidencias de las características técnicas del instrumento elaborado.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, May 20, 2014; With Addendums

Weekly letting report.

Intensidade de raleio de frutos em pessegueiros 'flordaprince' conduzidos em pomar com alta densidade de plantio

O objetivo deste trabalho foi verificar o comportamento de pessegueiros (Prunus persica (L.) Batsch.) cultivar Flordaprince, conduzidos em pomar com alta densidade (3.333 plantas/ha), submetidos a diferentes intensidades de raleio manual de frutos. Os tratamentos utilizados foram 120, 100 e 80 frutos por planta, e o controle sem raleio (230 frutos por planta). As plantas submetidas ao raleio produziram frutos significativamente maiores e mais pesados do que os do controle. A produção por planta (kg) e a produtividade estimada (t/ha) foram maiores nas plantas sem raleio, mas a classificação comercial e a receita bruta (R$/ha) desse tratamento foram menores, devido ao menor tamanho e peso dos frutos. Os tratamentos 100 e 80 frutos por planta (56,52% e 65,21% de raleio, respectivamente) apresentaram os melhores resultados

Exigências hídricas da videira na Região do Submédio São Francisco

O trabalho objetivou a estimativa das necessidades hídricas da videira (Vitis vinifera L.), cv. Itália, sob as condições edafoclimáticas da Região do Submédio São Francisco. A parte experimental foi conduzida no campo experimental de Bebedouro da Embrapa-Centro de Pesquisa Agropecuária do Trópico Semi-Árido, no município de Petrolina, PE, durante o período de maio a agosto de 1996. A evapotranspiração da cultura foi determinada pelo método do balanço hídrico no solo, e a evapotranspiração de referência foi estimada pelo método de Penman, visando avaliar o comportamento do coeficiente de cultura (Kc) ao longo do ciclo da cultura. O parreiral, com cinco anos de idade, foi conduzido em sistema de latada a 2 m acima da superfície do solo, num espaçamento de 4 m x 2 m e irrigado diariamente por gotejamento. O consumo hídrico diário máximo da cultura foi de 4,33 mm dia-1, totalizando 333,6 mm no período de observações. Os valores de Kc variaram de 0,50 a 0,74. Determinou-se uma curva característica de Kc para o ciclo vegetativo da videira, a qual permite obter o Kc diário em função dos dias após a poda.