Elicitation of specific cytotoxic T cells by immunization with malaria soluble synthetic polypeptides.


Autoria(s): Blum-Tirouvanziam U.; Beghdadi-Rais C.; Roggero M.A.; Valmori D.; Bertholet S.; Bron C.; Fasel N.; Corradin G.
Data(s)

1994

Resumo

We have studied the immunogenicity of Plasmodium falciparum circumsporozoite (CS) protein-derived synthetic polypeptides in mice. These synthetic peptides correspond to the N- and the C-terminal domains 22-125 and 289-390, respectively of the P. falciparum 7G8 isolate CS protein expressed on the sporozoite surface. They comprise what is believed to be the mature protein, except for the central repetitive B cell domain. BALB/c (H-2d) mice were immunized s.c. with 50 micrograms soluble CS polypeptides emulsified in IFA. After a single immunization, CS-specific helper and cytotoxic T lymphocytes (CTLs) could be obtained. The resultant CTLs obtained by in vitro restimulation of primed lymph node (LN) cells recognized H-2Kd target cells in the presence of short synthetic peptides defined in the present study. These epitopes are contained within the N- and C-terminal regions of the CS protein, and correspond to sequences 39-47 and 333-342. In addition, these CTLs can specifically lyse H-2d target cells transfected with the CS gene. These results suggest that, by immunization of mice with large soluble CS synthetic polypeptides in IFA, it is possible to obtain MHC class I-restricted T cell responses specific for the CS protein. This approach might be advantageous in the formulation of efficient malaria subunit vaccines.

Identificador

http://serval.unil.ch/?id=serval:BIB_C014428D5942

isbn:0022-1767 (Print)

pmid:7930617

isiid:A1994PN07100029

Idioma(s)

en

Fonte

Journal of Immunology, vol. 153, no. 9, pp. 4134-4141

Palavras-Chave #Amino Acid Sequence; Animals; H-2 Antigens/immunology; Malaria Vaccines/immunology; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Peptide Fragments/biosynthesis; Peptide Fragments/immunology; Protozoan Proteins/biosynthesis; Protozoan Proteins/immunology; Recombinant Proteins/biosynthesis; T-Lymphocytes, Cytotoxic/immunology; Transfection
Tipo

info:eu-repo/semantics/article

article