Influence of nitrogen source and sucrose concentration on inulinase production by Kluyveromyces marxianus in fed-batch fermentation

This work studied the influence of nitrogen source and sucrose concentration in the feeding medium for biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus. The results show that the best nitrogen source was a combination of 5 g/L of yeast extract and 10 g/L of peptone. Both cellular growth and enzymatic activity increased with sucrose concentration in the feeding medium (from 200 to 500 g/L). When the sucrose concentration reached 600 g/L, both cellular growth and enzymatic activity decreased.

Reactivity of mesenteric and aortic rings from trained rats fed with high caloric diet

A strong association between the benefits of physical exercise on the cardiovascular disease with an improvement of the endothelium-derived relaxing factor production has been consistently shown. The purpose of this study was to evaluate the effect of exercise training associated with high caloric diet in the reactivity of rat mesenteric and aortic rings. Experimental protocol consisted of 4 weeks of high caloric diet consumption previous to 4 weeks of run training (1.2 km/h, 0% grade, in sessions of 60 min, 5 days/week). Concentrations of triglycerides, glucose, insulin and nitrite/nitrate levels were measured and atherogenic index was calculated. Concentration-response curves to acetylcholine (10 nM-100 mu M), sodium nitroprusside (100 pM-100 nM) and phenylephrine (1 nM-3 mu M) were obtained. Exercise training reduced body mass (6%) and triglyceride levels (about 54%), without changes in glucose and insulin concentrations. An improvement of endothelium-dependent relaxation responses to acetylcholine in mesenteric and aortic rings was observed in trained group. No changes were seen for sodium nitroprusside and phenylephrine. In conclusion, our study is the first to show clearly that run training promotes an improvement of the endothelium-dependent relaxing response in aorta and mesenteric rings from rats fed with high caloric diet and that is associated with increase of NO production. (c) 2006 Elsevier B.V. All rights reserved.

Exercise test and glucose homeostasis in rats treated with alloxan during the neonatal period or fed a high calorie diet

Background: Animal models appear well-suited for studies into the role of exercise in the prevention of non-insulin-dependent diabetes mellitus (NIDDM). The aim of the present study was to analyze glucose homeostasis and blood lactate during an exercise swimming test in rats treated with alloxan during the neonatal period and/or fed a high calorie diet from weaning onwards.Methods: Rats were injected with alloxan (200 mg/kg, i.p.) or vehicle (citrate buffer) at 6 days of age. After weaning, rats were divided into four groups and fed either a balanced diet or a high-caloric diet as follows: C, control group (vehicle + normal diet); A, alloxan-treated rats fed the normal diet; H, vehicle-treated rats fed the high-caloric diet; and HA, alloxan-treated rats fed the high-caloric diet.Results: Fasting serum glucose levels were higher in groups A and AH compared with the control group. The Homeostatic Model Assessment index varied in the groups as follows: H > A > HA = C. There were no differences in free fatty acids or blood lactate concentrations during the swim test.Conclusions: Alloxan-treated rats fed a normal or high-caloric diet have the potential to be used in studies analyzing the role physical exercise plays in the prevention of NIDDM.

Exercise counteracts fatty liver disease in rats fed on fructose-rich diet

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Platelet hyperaggregability in high-fat fed rats: A role for intraplatelet reactive-oxygen species production

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Giant monopole resonance and nuclear compression modulus for Ca-40 and O-16

Using a collective potential derived previously on the basis of the generator coordinate method with Skyrme interactions, we obtain values for the compression modulus of Ca-40 which are in good agreement with a recently obtained experimental value. Calculated values for the compression modulus for O-16 are also given. The procedure involved in the derivation of the collective potential is briefly reviewed and discussed.

Commissioning and performance of the CMS silicon strip tracker with cosmic ray muons

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Corrugated waveguide under scaling investigation

Some scaling properties for classical light ray dynamics inside a periodically corrugated waveguide are studied by use of a simplified two-dimensional nonlinear area-preserving map. It is shown that the phase space is mixed. The chaotic sea is characterized using scaling arguments revealing critical exponents connected by an analytic relationship. The formalism is widely applicable to systems with mixed phase space, and especially in studies of the transition from integrability to nonintegrability, including that in classical billiard problems.

Finding invariant tori in the problem of a periodically corrugated waveguide

Some dynamic properties for a light ray suffering specular reflections inside a periodically corrugated waveguide are studied. The dynamics of the model is described in terms of a two dimensional nonlinear area preserving map. We show that the phase space is mixed in the sense that there are KAM islands surrounded by a large chaotic sea that is confined by two invariant spanning curves. We have used a connection with the Standard Mapping near a transition from local to global chaos and found the position of these two invariant spanning curves limiting the size of the chaotic sea as function of the control parameter.

Characterization of multiple reflections and phase space properties for a periodically corrugated waveguide

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Scaling invariance for the escape of particles from a periodically corrugated waveguide

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Effect of the addition of invert sugar on the production of cephalosporin C in a fed-batch bioreactor

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)