Performing "Up The Shore": a stylistic analysis of Newfoundland Irish English vowels in the Ferryland dinner theatre

This study is a variationist sociolinguistic analysis of two speech styles, performance and interview, of a dinner theatre troupe in Ferryland on the Southern Shore of Newfoundland. Five actors and ten of their characters are analyzed to test if their vowels change across styles. The study adopts a variationist framework with a Community of Practice model, drawing on Bell’s audience and referee design to argue that the performers’ stage conventions and identity construction are influenced by a third person referee: the Idealized Authentic Newfoundlander (IAN). Under this view the goal of the performer is to both communicate with and entertain the audience, which requires different tactics when speaking. These tactics manifest phonetically and are discussed in a quantitative, statistical analysis of the acoustic measurements of the vowel tokens [variables FACE, KIT, LOT/PALM and GOAT lexical sets with Newfoundland Irish English (NIE) variants] and a qualitative discussion.

Utilização de peptídeos sintéticos derivados de moléculas imunodominantes de Toxoplasma gondii para diagnóstico sorológico da toxoplasmose em ovinos e galinhas caipiras

Chapter I - The obligate intracellular parasite Toxoplasma gondii is the causative agent of toxoplasmosis, a disease that affects humans and generates economic losses in farm animals. When prevention fails disease refers to the diagnosis and subsequent treatment if the individual is diagnosed as positive. Therefore, the development of new accurate diagnostic tools for detecting T. gondii infection is a need in particular to determine the environmental source of infection which can result in more appropriate public health policies against different routes of infection and prevent potential damage that toxoplasmosis can cause when animals are infected. Chapter II - The domestic chicken (Gallus gallus domesticus) are considered epidemiological sentinels, still representing a major source of recombinant strains when predated by cats, it is common to find them with multiple infections. We evaluate the diagnostic potential of six synthetic peptides (SAG2Y, MIC1, M2AP, GRA10, ROP2 and ROP7) predicted in silico from tachyzoites immunodominant markers of T. gondii in samples from naturally infected chickens, comparing synthetic peptides with antigen total soluble (STAg). In general, our results demonstrated that reactivity rates and positivity for these peptides are similar to the STAg, and the ROP7 peptide and the combination of peptides MIC1+ROP2 have significant sensitivity, confirming them as potential diagnostic tools for the diagnosis of toxoplasmosis in chickens. Chapter III - Sheep (Ovis aries) are commonly infected with Toxoplasma gondii due to his eating habits. Infection in pregnant sheep can have serious consequences such as embryonic death, fetal resorption, mummification, and neonatal death. One concern regarding the infection in these animals is that the meat can be a source of contamination to humans and other carnivores. Therefore perform accurate diagnosis in these animals is of fundamental importance. In the present study we evaluated the potential of new synthetic peptides as a diagnostic tool. Synthetic peptides (SAG2Y, SRS52A, MIC14, GRA4, GRA10 and GRA15) were predicted in silico from immunodominant proteins of T. gondii. We determine the levels of IgG antibodies using sera obtained from two farms in the city of Uberlândia. Analyzing the results together, the peptide combination GRA10+GRA15 (Accuracy = 0,82) showed better characteristics compared with the other mixtures. This preparation could be better analyzed with an antigenic mixture potential use in the diagnosis of toxoplasmosis in sheep and other species.

Luz e vida: uma proposta de formação continuada de professores

The Course “Light and Life”, which is a product of the present research, is a mediator in helping Science Teachers on how to deal with the Physics classes in secondary school. The aim of the present study was to propose a course which can help Science Teachers with their difficulties in the teaching of Physics in the later years of secondary school, mainly about topics related to the theme Light. The elaboration of the product of this research involved structuring and applying a course of continued formation through the “Fun with Science and Art Museum-DICA, under the theme Light, to Science Teachers of the later years of secondary school, in order to promote an environment of dialogue and problem raising as means of disseminating and discussing the teaching of topics related to Light in the teaching of Sciences. The formation course presented a flexible structure in order to widen the relation between the researcher and the teachers participating in the course, aiming to give an answer to the formative needs of the teachers in relation to the theme proposed. In order to know and discuss the problems and challenges of the teaching of Physics in the Science classes, an approach of insertion and integration of concepts related to the theme Light was conducted, mainly on topics relating to the contents of Physics, Chemistry and Biology, according to the curriculum and the abilities of Sciences worked on in class, through the approach of concepts and practice which approximate the practical and theoretical reality of the formative needs of the teachers involved in this study. This work aimed to understand the relation of Science Teachers with the theme Light and comprehend the relation of the teachers with the present proposal of continued formation promoted by the Museum DICA. The methodological option of this research remained in the domains of qualitative research whose analysis was based on the content analysis. The data collection was carried out through questionnaires and group discussions recorded in audio, besides the participative observation of the researcher. As the research product, the structuring and application of a course of continued formation of Science Teachers of secondary school was proposed, under the thematic Light, and a later restructuring of the course under the same thematic based on the data collected after the application of the referred course, which will be later promoted by the Museum DICA.

Hur grafiska formgivare runt om i världen uppfattar skandinavisk grafisk design

Syftet med föreliggande studie har varit att kartlägga hur grafiska formgivare runt om i världen uppfattar skandinavisk grafisk design. 53 deltagare från industriländer deltog i studien som använde en kombination av mail-intervjuer och enkäter. Resultaten från denna studie indikerar att, oavsett kontinent, upplevs skandinavisk grafisk design som enkel och funktionell. Layouten uppfattades som rutnätsbaserad med mycket ljusrum och få grafiska element. Monokroma färger som svart, vit och grå utan gradienter och skuggor uppfattades som typiska för skandinavisk grafisk design; följt av jord- och pastellfärger. Sanserifer var mest förknippade med skandinavisk grafisk design. Motiv ansågs i denna studie att användas sparsamt, men när de används avbildar de naturen eller geometriska former. Foton och illustrationer ansågs användas ungefär lika mycket, illustrationer hade en smärre större preferens. Den upplevda påverkan av skandinavisk grafisk design varierade mellan deltagarna. Deltagarna som tyckte att påverkan var stor, ansåg att detta berodde på förespråkandet av enkelhet och funktion, sammanflätning av designområden och/eller frammaning av hållbar grafisk design. Deltagarna som ansåg att påverkan var låg, tyckte att för lite publicitet var en bidragande faktor.De flesta deltagarna i denna undersökning ansåg att skandinavisk grafisk design var enklare i jämförelse med vad de kunde se i sina hemländer. Vidare tyckte afrikanska, asiatiska och sydamerikanska deltagare  att färgerna hade lägre kroma.

Monsters Take to the Streets! Monstrous Street-Art as Pedagogy of Resistance to Post-Olympic Regeneration in Hackney Wick?

This chapter explores geographies of gentrification and resistance in relation to the monstrous through the lens of street-art in post-Olympic London. It takes as a geographic case study Hackney Wick, which has for a long time been a bastion of alternative and creative living due to cheap rents in large, ex-industrial warehouse spaces. The artistic sociality of the area is imbued within its landscape, as prolific street artists have adorned ex-industrial warehouses and canal-side walls with graffiti and murals. Since the announcement of the 2012 Olympic Games, the area has been a site of intense political and aesthetic contestation. The post-Olympic legacy means that the area has been earmarked for redevelopment, with current residents facing the possibility of joining thousands already displaced by the games. The anxiety of dispossession is reflected by monstrous characters and sinister disembodied teeth, eyes and fingers embedded within the landscape, painted by local artists. Using geographically sensitive mobile and visual methodology to document the landscape and artwork, the chapter analyses and interprets the monstrous themes using a range of theorists including Mikhail Bakhtin, Georges Bataille and Nick Land. I argue that monstrous street-art lays visible claim to public territory for aesthetic purposes at odds with the visions of redevelopers and the needs of capital. Whilst street-art and graffiti do not fit easily within frameworks of organized political resistance or collective social movements, they operate as a kind of epistemological transgression that triggers transformative affects in the viewer. This creates conditions for pedagogies of resistance to gentrification by expressing and mobilizing political affects such as anger and anxiety, raising awareness of geographical politics, and encouraging the viewer to question the status quo of the built environment.

Monitorização e gestão em turismo : uma proposta de dashboard visual

A presente investigação propõe-se a atuar no sector turístico, uma vez que este é bombardeado diariamente por uma quantidade considerável de dados e informações. Atualmente, usufrui-se significativamente mais da tecnologia com a finalidade de promover e vender os produtos/serviços disponíveis no mercado. A par da evolução tecnológica, os utilizadores/clientes conseguem comprar, cada vez mais, à distancia de um clique os produtos turísticos que desejam. No entanto, há um variado leque de aplicações sobre o turismo que permitem entender os gostos e as necessidades dos turistas assim como a sua atitude para com o mesmo. Porém, nem as entidades nem os gestores turísticos usufruem inteligentemente dos dados que lhes são facultados. Estes tendem normalmente a prender-se pelo turismo em Portugal e de que forma é que a sua entidade é apresentada acabando por esquecer que os dados podem e devem ser utilizados para expandir o mercado assim como entender/conhecer potenciais mercados. Deste modo, o fundamento principal desta investigação remete para a criação de uma plataforma infocomunicacional que analise na totalidade os dados obtidos, assim como fornecer as ferramentas pertinentes para que se consiga fazer esta análise, nomeadamente através de uma representação infográfica adequada e estratégias de a comunicar aos stakeholders.. Para tal foi aplicada no âmbito desta dissertação a metodologia investigação/ação, vista como um processo cíclico que para além de incluir simultaneamente estas duas vertentes, vai alternando entre a ação e a reflexão critica sendo sustentada por bases teóricas. A criação do protótipo da plataforma Smart Tourism, resultou num sistema inovador que tenta responder aos indicadores escolhidos no Dashbord e ao problema infocomunicacional, tentando criar as bases necessárias para que as entidades consigam analisar de forma mais integrada/sistematizada e racional a atividade turística. Foi por isso, desenvolvido e avaliado qualitativamente um protótipo de base infocomunicacional visual (dashboard visual) que para além do que para além do que já foi referido, consegue proporcionar a gestão dos produtos, clientes, staff e parceiros, aumentando assim o valor deste sector.

Cooperar com os PAIS, um sinal da mudança dos tempos

Relatório de Estágio apresentado à Escola Superior de Educação de Paula Frassinetti para obtenção do grau de Mestre em Educação Pré- Escolar.RENATES TID 201004437

Technical Services vs. Public Service

Regarding this issue, it seems appropriate to quote Gorman who says: And it came to pass that when Kutta, assistant God made the first library, she noted that it was good. So he called together all librarians and divided as a shepherd divides the sheep and goats. The first group spoke to him saying: you will dwell in the clarity and serve the readers and their glory will be great. Then he turned to the second group and spoke to him saying: you will dwell in the darkness. Secret must be hidden effort and work. You do not know the reader nor shall you know them. Go away and sort ... and has been up to these days (1979, p. 435).

Estudo da imobilização das proteases aspárticas da flor de Cynara cardunculus L. Reologia da coalhada

Mestrado em Biotecnologia, Faculdade de Engenharia de Recursos Naturais, Univ. do Algarve, 2005

Evaluación de la exposición al ruido industrial en los trabajadores de una planta de asfalto, en la provincia del Azuay y propuesta de plan de control

El presente trabajo se realizó en una Planta de Hormigón Asfáltico, donde se realizó el estudio del ruido como factor de riesgo bajo las perspectivas de: Salud Ocupacional, Seguridad e Higiene Industrial. Este enfoque holístico, define la influencia que éste riesgo laboral ejerce sobre la pérdida de agudeza auditiva. Se estableció inicialmente el marco teórico y legal vigente sobre el ruido, posteriormente se describió la metodología de estudio, operatividad de variables, y la muestra a estudiar. Luego se procedió a la caracterización de la población y área estudiada, así como las actividades productivas. Dentro de la Higiene Industrial, se monitoreó la exposición del nivel de presión sonora, se realizó el comparativo con el nivel permisible (TWA 8 horas) establecido en el “Reglamento de Seguridad y Salud de los Trabajadores”, D.E. 2393. En términos de Seguridad Industrial, se analizó el equipo de protección personal EPP utilizado, y las variables determinantes. En el ámbito de Salud Ocupacional, se estudió: características personales de trabajadores, patologías relacionadas con sordera, y un cuestionario de exposición al ruido. Finalmente se determinó la existencia de sobreexposición a ruido laboral en la empresa objeto de estudio, mediante el estudio del estado de salud auditiva de los colaboradores se determinó la gran incidencia de personal sano y finalmente se determinó las medidas de control a implementarse enfatizadas a la realidad descubierta en el presente estudio, las que incluyen cambios en equipos y maquinaria, buenas prácticas de trabajo, planes de adiestramiento y capacitación en todo el personal entre otros.

The healing power of grandparents who parent

This paper explores the changing role of contemporary grandparents with many demonstrating a willingness and ability to take on parental responsibilities for their grandchildren, where they may face challenges and opportunities in difficult times. Three main forms of grand parenting are identified in the literature, those who hâve primary responsibility and are raising their grand children as their main carers perhaps in response to crisis situations, those who live in extended families and participate in care, and those who provide day care while the child's parents work. The rôle of Grandparents encompasses ail three areas in the United Kingdom. This has occurred as a resuit of the increased récognition of children living in abusive families, of the increasing frequency of divorce, single parenting, children and families living in poverty, socio-economic deprivation and the lack of available or subsidised child care in the United Kingdom when parents hâve to work to support the family. Ail of this against the backdrop the Every Child Matters change for children Agenda. When grandparents step into a troubled situation and attempt to offer stability and security for their grandchildren they may hâve to manage the combined responsibilities of family caregivers and parental figures. Grandparenthood is atenuous rôle, lacking clear agreement on behaviour norms. In the current culture of advice and parenting support, while care must be taken not to undermine parenting skills or make judgements about the ability to cope with the demands of childcare, an exploration of the impact on grandparents, and children in the context of families must be undertaken. Due to the complex web of interrelated factors the process and outcomes of care giving by grandparents is not well known in the literature. It is proposed therefore that it is timely to explore the impact of this on the health of children and their families in the current climate of change when there has been dissolution of the nuclear family and grandparents are playing increasingly significant rôles in healing and supporting their families.

Positive biocompatibility of nanocrystalline glass-like carbon thin films

The interest in carbon nanomaterials with high transparency and electrical conductivity has grown within the last decade in view of a wide variety of applications, including biocompatible sensors, diagnostic devices and bioelectronic implants. The aim of this work is to test the biocompatibility of particular nanometer-thin nanocrystalline glass-like carbon films (NGLC), a disordered structure of graphene flakes joined by carbon matrix (Romero et al., 2016). We used a cell line (SN4741) from substantia nigra dopaminergic cells derived from transgenic mouse embryo cells (Son et al., 1999). Some cells were cultured on top of NGLC films (5, 20 and 80 nm) and other with NGLC nanoflakes (approx. 5-10 mm2) in increasing concentrations: 1, 5, 10, 20 and 50 μg/ml, during 24 h, 3 days and 7 days. Cells growing in normal conditions were defined under culture with DMEM supplemented with 10% FCS, Glucose (0,6%), penicillin-streptomycin (50U/ml) and L-glutamine (2mM) at 5%CO2 humidified atmosphere. Nanoflakes were resuspended in DMEM at the stock concentration (2 g/l). The experiments were conducted in 96 well plates (Corning) using 2500 cells per well. For MTT analysis, the manufacturer recommendations were followed (Roche, MTT kit assay): a positive control with a 10% Triton X-100 treatments (15 minutes) and a negative control without neither Triton X-100 nor NGLC. As apoptosis/necrosis assay we used LIVE/DEAD® Viability/Cytotoxicity Assay Kit (Invitrogen). In a separate experiment, cells were cultured on top of the NGLC films for 7 days. Primary antibodies: anti-synaptophysin (SYP, clone SY38, Chemicon) and goat anti-GIRK2 (G-protein-regulated inward-rectifier potassium channel 2 protein) (Abcom) following protocol for immunofluorescence. WB for proteins detection performed with a polyclonal anti-rabbit proliferating cell nuclear antigen (PCNA). Results demonstrated the biocompatibility with different concentration of NGLC varying the degree of survival from a low concentration (1 mg/ml) in the first 24 h to high concentrations (20-50 g/ml) after 7 days as it is corroborated by the PCNA analysis. Cells cultured on top of the film showed after 7 days axonal-like alignment and edge orientation as well as net-like images. Neuronal functionality was demonstrated to a certain extent through the analysis of coexistence between SYP and GIRK2. In conclusion, this nanomaterial could offer a powerful platform for biomedical applications such as neural tissue engineering

Ocorrência da infecção por Toxoplasma gondii e avaliação da imunização em caprinos do sertão do cabugi, Rio Grande do Norte

Toxoplasmosis is one zoonosis caused by Toxoplasma gondii protozoan. Goats, amongst the production animals, are one of the species most susceptible to this parasite, being one them main involved agents in ovine and goat abortions, determining great economic losses and implications for public health, since the presence it parasite in the products of goat origin, consist in one of the main sources of infection for the man. In this study 244 blood samples in 8 farms situated in 4 cities from the Sertão do Cabugi region, Rio Grande do Norte State, northeast of Brazil and, tested by ELISA assay. The results had shown a prevalence of 47.13% for anti- T. gondii antibodies and a significant association between positivity and variable evaluated as age, locality and property. The IgG avidity assay evaluated in 115 positive samples was carried to discriminate acute and chronic infection. Twelve samples (10.4%) had presented antibodies of low avidity while 103 (89.6%) presented high avidity antibodies; indicating that most of the animals was precocious exposure to the parasite. Significant difference was verified only for the variable sex. We also evaluate the capacity of recombinant adenoviruses codifying SAG1, SAG2, SAG3 and CMV in inducing activation of specific immune response in goat. These 109 animals received 109 pfu of the AdSAG1, AdSAG2, AdSAG3, AdCMV or PBS in vaccine protocol with 3 immunizations. Serum samples of the each animal, before and after mmunization, had been submitted to the ELISA. The results demonstrate that the immunizations had induced the production of IgG antibodies specific against T. gondii proteins

When the virtual influences reality. The conservation project of the toledo gate in ciudad real (Spain)

The Toledo Gate of Ciudad Real, Spain, constructed between the late 13th and early 14th centuries, is the last remaining portion of a once complete medieval city wall. It represents the long history of the city and constitutes its main heritage symbol, dividing the historic city centre from the later 19th and 20th century expansions. In October 2012, the Town Hall and the Montemadrid Foundation started the conservation works to preserve this important monument. The preliminary phase of this project included an in-depth series of scientific studies which were carried out by a multidisciplinary team focusing on archival research, historic investigations, archaeological excavations as well as material composition analysis and main treatment application tests. As a result of these studies a series of virtual 3D models were created to inform, discuss and study the monument. A first digital model permitted visualization of the gate in the 19th century and how the main entrance to the city was integrated as a fundamental part of the city walls. This virtual reconstruction also became an important part of the campaign to raise awareness among the citizens towards a monument that had remained in the shadows for the last century, isolated in a roundabout after the systematic demolition of the city walls in the late 19th century. Over the last three years and as a result of these archaeological and historic investigations and subsequent virtual models, surprisingly new and interesting data were brought to light thus permitting the establishment and corroboration of a new and updated hypothesis of the Toledo Gate that goes beyond the previous ideas. As a result of these studies a new architectural typology with construction techniques of has been suggested. This paper describes how the results of this continuous and interdisciplinary documentation process have benefitted from a computer graphic reconstruction of the gate. It highlights how virtual reconstruction can be a powerful tool for conservation decision making and awareness raising. Furthermore, the interesting results of the final reconstruction hypothesis convinced the technical team responsible for the conservation to alter some aspects of the final project physical interventions in order to focus on some of the features and conclusions discovered through the virtual model study.

Reforestation challenges in Scandinavia

In the keynote, major reforestation challenges in Scandinavia will be highlighted. The following countries make up Scandinavia: Iceland, Norway, Sweden, Finland and Denmark. For Iceland, with only a forest cover of 2%, a major reforestation challenge is the deforestation and overgrazing in combination with land degradation and extensive soil erosion. The challenges include the conflicts with livestock farmers. For centuries the commons were used for sheep and horse grazing. However, more and more of farmer grazing land have been fenced up, allowing the regeneration of birch and plantations of other species to increase. With a forest cover of 37% and 69% respectively, for decades a major reforestation challenge in Norway and Sweden has been the risk of seedling damages from the pine weevil. Unprotected seedlings can have a survival rate of less than 25% after being planted. Pine weevils feed on the bark of planted young seedlings at regeneration sites. If the seedling is girdled, it will not survive. In Sweden, and soon in Norway, pesticides have been forbidden. In the keynote, new methods and technology will be presented based on non-chemical protection. In Finland, with a forest cover of 75%, a major reforestation challenge is linked to the forest structure. The structure of Finnish forestry includes many private forests in combination with small regeneration sites. This implies a situation where logistics and methods for lifting and field storage provide a major challenge in order to preserve seedling quality until the planting date. Due to this situation, new logistic systems and technologies are being developed in Finland, including new seedling cultivation programs (including cultivation under Light Emitting Diodes (LEDs)) to match the access of fresh planting stock to different planting dates. In Denmark, with a forest cover of 13%, a major reforestation challenge is the possibility of future plantations based on a wide range of relevant species. For this to become a realistic option, new methods and technology have to be developed in reforestation activities that support this possibility. These methods and technology should make it possible to not be limited to certain species due to problems and restrictions during field establishment. This due to the prospect of establishing stable, healthy, and productive stands of various forest species that can be adapted to future climate change.