Lab on paper: biossensores colorimétricos de papel para a detecção de membros do complexo Mycobacterium tuberculosis

Este trabalho foi realizado no âmbito do projecto Lab on Paper, desenvolvido no Centro de Investigação de Materiais (CENIMAT) da Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa (FCT - UNL)

Management consulting lab- Galp social media

This study consists of the reflection on a consultancy project developed by four students and one project manager from NOVA SBE. In attempting to assist Galp Energia structure the operationalization of an entry into Social Media, we were confronted with first-time challenges in real-life highly demanding workplace situations. The following considerations attempt to defuse the problem-solving mindset of the practical experience from the methodological development and learning experience extracted from the consulting line of work

Management consulting lab-Portugal Telecom

This paper presents the main developments and learning taken from the Management Consulting Lab at Portugal Telecom. The main purpose of this consulting project was to assess the potential of a specific technology and how could Portugal Telecom maximize the value created. By identifying and evaluating all the business sectors where this technology would have impact, the team was able to address the initial hypotheses stated by the client regarding the importance of the technology and elaborate a set of recommendations based on the main findings obtained through field as well as desk research.

Evaluating balance, stability and gait symmetry of stroke patients using kinematic and dynamic gait lab data

Dissertação de mestrado integrado em Engenharia Biomédica

Kids Media Lab: Tecnologias e a aprendizagem da programação em idade Pré-escolar

Sabedores da realidade tecnológica que envolve as crianças, sentimos necessidade de conhecer aprofundadamente como é que as crianças aprendem, ou seja, como estruturam e desenvolvem o seu pensamento, nesta relação com as tecnologias e através da programação. A presente investigação revela-se inovadora, quer no contexto em que vai ser implementado, quer nos objetivos da própria investigação. Pretendemos consolidar conhecimento sobre as teorias de aprendizagem, através da introdução da programação de forma lúdica e criativa nas atividades no jardim de infância. Compreender como as crianças reagem na utilização das tecnologias e da programação revela-se importante para conhecer o seu comportamento, o seu estilo de aprendizagem e permitir aos profissionais de educação antecipar ou não esta integração, nos seus contextos educativos. Conhecer como todo este processo de aprendizagem é vivenciado pelas crianças irá permitir avaliar quais os benefícios e riscos da utilização das tecnologias e da aprendizagem da programação em idade pré-escolar.

PDMS encasing system for integrated lab-on-chip Ag/AgCl reference electrodes

Poly(dimethylsiloxane) (PDMS) is an organosilicon polymer widely used in the fabrication of microfluidic systems to integrate biochips. In this study, we propose the use of an adapted PDMS mould for the creation of a miniaturized, reusable, reference electrode for in-chip electrochemical measurements. Through its integrated microfluidic system it is possible to replenish internal buffer solutions, unclog critical junctions and treat the electrode’s surface, assuring a long term reuse of the same device. Planar Ag/AgCl reference electrodes were microfabricated over a passivated p-type Silicon Wafer. The PDMS mould, containing an integrated microfluidic system, was fabricated based on patterned SU-8 mould, which includes a lateral horizontal inlet access point. Surface oxidation was used for irreversible permanent bondage between flat surfaces. The final result was planar Ag/AgCl reference electrode with integrated microfluidic that allows for electrochemical analysis in biochips

Outlines of lectures on materia medica and botany :delivered in Jefferson Medical College, Philadelphia /by William P.C. Barton.

v.2 (1827)

Outlines of lectures on materia medica and botany :delivered in Jefferson Medical College, Philadelphia /by William P.C. Barton.

v.1 (1827)

Self-Confidence, Overconfidence and Prenatal Testosterone Exposure: Evidence from the Lab

This paper examines whether the degree of confidence and overconfidence in one's ability is determined biologically. In articular, we study whether foetal testosterone exposure correlates with an incentive-compatible measure of confidence within an experimental setting. We find that men (rather than women) who were exposed to high testosterone levels in their mother's womb are less likely to overestimate their actual performance, which in turn helps them to gain higher monetary rewards. Men exposed to low prenatal testosterone levels, instead, set unrealistically high expectations which results in self-defeating behaviour. These results from the lab are able to reconcile hitherto disconnected evidence from the field, by providing a link between traders'overconfidence bias, long-term financial returns and prenatal testosterone exposure.

Validation d'une nouvelle grille de codage de la régulation émotionnelle avec le Laboratory Temperament Assessment Battery (Lab-TAB)

L'objectif de cette étude est de valider une nouvelle grille de codage pour le Lab-TAB (Goldsmith et Rothbart, 1999). Cette grille mesure l'intensité émotionnelle et les comportements de régulation chez l'enfant âgé de six mois. L'accord interjuge se révèle satisfaisant. Les analyses factorielles ont mis en évidence trois facteurs - attention, niveau d'activité et détournement actif - retrouvés dans la littérature comme stratégies de régulation émotionnelle. Les analyses de consistance interne démontrent une bonne homogénéité de ces facteurs. La validité externe se révèle satisfaisante aux niveaux concourant et prédictif. Enfin, cet outil de codage permet de distinguer les prématurés et les nés à terme. L'ensemble de ces résultats indiquent que l'outil élaboré présente de bonnes qualités psychométriques.

Bioconjugated lanthanide luminescent helicates as multilabels for lab-on-a-chip detection of cancer biomarkers.

The lanthanide binuclear helicate [Eu(2)(L(C2(CO(2)H)))(3)] is coupled to avidin to yield a luminescent bioconjugate EuB1 (Q = 9.3%, tau((5)D(0)) = 2.17 ms). MALDI/TOF mass spectrometry confirms the covalent binding of the Eu chelate and UV-visible spectroscopy allows one to determine a luminophore/protein ratio equal to 3.2. Bio-affinity assays involving the recognition of a mucin-like protein expressed on human breast cancer MCF-7 cells by a biotinylated monoclonal antibody 5D10 to which EuB1 is attached via avidin-biotin coupling demonstrate that (i) avidin activity is little affected by the coupling reaction and (ii) detection limits obtained by time-resolved (TR) luminescence with EuB1 and a commercial Eu-avidin conjugate are one order of magnitude lower than those of an organic conjugate (FITC-streptavidin). In the second part of the paper, conditions for growing MCF-7 cells in 100-200 microm wide microchannels engraved in PDMS are established; we demonstrate that EuB1 can be applied as effectively on this lab-on-a-chip device for the detection of tumour-associated antigens as on MCF-7 cells grown in normal culture vials. In order to exploit the versatility of the ligand used for self-assembling [Ln(2)(L(C2(CO(2)H)))(3)] helicates, which sensitizes the luminescence of both Eu(III) and Tb(III) ions, a dual on-chip assay is proposed in which estrogen receptors (ERs) and human epidermal growth factor receptors (Her2/neu) can be simultaneously detected on human breast cancer tissue sections. The Ln helicates are coupled to two secondary antibodies: ERs are visualized by red-emitting EuB4 using goat anti-mouse IgG and Her2/neu receptors by green-emitting TbB5 using goat anti-rabbit IgG. The fact that the assay is more than 6 times faster and requires 5 times less reactants than conventional immunohistochemical assays provides essential advantages over conventional immunohistochemistry for future clinical biomarker detection.