Isolation of Aeromonas hydrophila in piglets

The production of Alentejano breed pig started a recovery two decades ago due to increasing demand for gourmet products. These pigs are raised in rotational semi-extensive or extensive outdoor production systems in the “Montado” (green and cork oak forest), grazing and feeding acorns and other associated food resources. Bacteria of the genus Aeromonas are considered as emerging pathogens of importance for man and animals, but its involvement in swine is not well documented. In the context of a study made at the University of Évora to assess the specific diseases of Alentejano swine, diseased piglets from two farms were submitted for pathological and bacteriological examinations. Pathological examinations revealed changes characteristic of septicemia, and Aeromonas hydrophila was isolated in pure culture from multiple organs of piglets from both farms. Antibiotic sensitivity tests showed that the isolates from one of the farms were susceptible to gentamicin, oxitetracycline, neomycin, enrofloxacin, colistin sulfate, trimethoprim, ceftiofur, and amoxicillin plus clavulanic acid. In contrast, the A. hydrophila isolated in the other farm was resistant to all drugs tested but enrofloxacin. This is the first report in the world showing the relationship between septicemia and A. hydrophila infection in piglets. The importance of this finding is further reinforced by the fact that these bacteria can be highly resistant to antimicrobial agents.

A representação de uma árvore para uma nova forma de ver o tempo e o espaço : desenho A 12º ano

Relatório da prática de ensino supervisionada, Mestrado em Ensino de Artes Visuais, Universidade de Lisboa, 2014

A normative framework for agent-based systems

One of the key issues in the computational representation of open societies relates to the introduction of norms that help to cope with the heterogeneity, the autonomy and the diversity of interests among their members. Research regarding this issue presents two omissions. One is the lack of a canonical model of norms that facilitates their implementation, and that allows us to describe the processes of reasoning about norms. The other refers to considering, in the model of normative multi-agent systems, the perspective of individual agents and what they might need to effectively reason about the society in which they participate. Both are the concerns of this paper, and the main objective is to present a formal normative framework for agent-based systems.

The attractive female body weight and female body dissatisfaction in 26 countries across 10 world regions: results of the International Body Project I

This study reports results from the first International Body Project (IBP-I), which surveyed 7,434 individuals in 10 major world regions about body weight ideals and body dissatisfaction. Participants completed the female Contour Drawing Figure Rating Scale (CDFRS) and self-reported their exposure to Western and local media. Results indicated there were significant cross-regional differences in the ideal female figure and body dissatisfaction, but effect sizes were small across high-socioeconomic-status (SES) sites. Within cultures, heavier bodies were preferred in low-SES sites compared to high-SES sites in Malaysia and South Africa (ds = 1.94-2.49) but not in Austria. Participant age, body mass index (BMI), and Western media exposure predicted body weight ideals. BMI and Western media exposure predicted body dissatisfaction among women. Our results show that body dissatisfaction and desire for thinness is commonplace in high-SES settings across world regions, highlighting the need for international attention to this problem.

3D Reconstruction of Medical Images from Slices Automatically Landmarked with Growing Neural Models

In this study, we utilise a novel approach to segment out the ventricular system in a series of high resolution T1-weighted MR images. We present a brain ventricles fast reconstruction method. The method is based on the processing of brain sections and establishing a fixed number of landmarks onto those sections to reconstruct the ventricles 3D surface. Automated landmark extraction is accomplished through the use of the self-organising network, the growing neural gas (GNG), which is able to topographically map the low dimensionality of the network to the high dimensionality of the contour manifold without requiring a priori knowledge of the input space structure. Moreover, our GNG landmark method is tolerant to noise and eliminates outliers. Our method accelerates the classical surface reconstruction and filtering processes. The proposed method offers higher accuracy compared to methods with similar efficiency as Voxel Grid.

Nutritional Evaluation and Optimisation in Neonates: a randomized, double-blind controlled trial of amino acid regimen and intravenous lipid composition in preterm parenteral nutrition

Background: Parenteral nutrition is central to the care of very immature infants. Current international recommendations favor higher amino acid intakes and fish oil–containing lipid emulsions. Objective: The aim of this trial was to compare 1) the effects of high [immediate recommended daily intake (Imm-RDI)] and low [incremental introduction of amino acids (Inc-AAs)] parenteral amino acid delivery within 24 h of birth on body composition and 2) the effect of a multicomponent lipid emulsion containing 30% soybean oil, 30% medium-chain triglycerides, 25% olive oil, and 15% fish oil (SMOF) with that of soybean oil (SO)-based lipid emulsion on intrahepatocellular lipid (IHCL) content. Design: We conducted a 2-by-2 factorial, double-blind, multicenter randomized controlled trial. Results: We randomly assigned 168 infants born at ,31 wk of gestation. We evaluated outcomes at term in 133 infants. There were no significant differences between Imm-RDI and Inc-AA groups for nonadipose mass [adjusted mean difference: 1.0 g (95% CI: 2108, 111 g; P = 0.98)] or between SMOF and SO groups for IHCL [adjusted mean SMOF:SO ratio: 1.1 (95% CI: 0.8, 1.6; P = 0.58]. SMOF does not affect IHCL content. There was a significant interaction (P = 0.05) between the 2 interventions for nonadipose mass. There were no significant interactions between group differences for either primary outcome measure after adjusting for additional confounders. Imm-RDI infants were more likely than Inc-AA infants to have blood urea nitrogen concentrations .7 mmol/L or .10 mmol/L, respectively (75% compared with 49%, P , 0.01; 49% compared with 18%, P , 0.01). Head circumference at term was smaller in the Imm-RDI group [mean difference: 20.8 cm (95% CI: 21.5, 20.1 cm; P = 0.02)]. There were no significant differences in any prespecified secondary outcomes, including adiposity, liver function tests, incidence of conjugated hyperbilirubinemia, weight, length, mortality, and brain volumes. Conclusion: Imm-RDI of parenteral amino acids does not benefit body composition or growth to term and may be harmful. This trial was registered at www.isrctn.com as ISRCTN29665319 and at eudract.ema.europa.eu as EudraCT 2009-016731-34.

Addressing software impact in the design of remote laboratories

Remote Laboratories or WebLabs constitute a first-order didactic resource in engineering faculties. However, in many cases, they lack a proper software design, both in the client and server side, which degrades their quality and academic usefulness. This paper presents the main characteristics of a Remote Laboratory, analyzes the software technologies to implement the client and server sides in a WebLab, and correlates these technologies with the characteristics to facilitate the selection of a technology to implement a WebLab. The results obtained suggest the adoption of a Service Oriented Laboratory Architecture-based approach for the design of future Remote Laboratories so that client-agnostic Remote Laboratories and Remote Laboratory composition are enabled. The experience with the real Remote Laboratory, WebLab-Deusto, is also presented.

Vitamin D deficiency and a CYP27B1-1260 promoter polymorphism are associated with chronic hepatitis C and poor response to interferon-alfa based therapy

BACKGROUND: Vitamin D is an important immune modulator and preliminary data indicated an association between vitamin D deficiency and sustained virologic response (SVR) rates in patients with chronic hepatitis C. We therefore performed a comprehensive analysis on the impact of vitamin D serum levels and of genetic polymorphisms within the vitamin D cascade on chronic hepatitis C and its treatment. METHODS: Vitamin D serum levels, genetic polymorphisms within the vitamin D receptor and the 1α- hydroxylase were determined in a cohort of 468 HCV genotype 1, 2 and 3 infected patients who were treated with interferon-alfa based regimens. RESULTS: Chronic hepatitis C was associated with a high incidence of severe vitamin D deficiency compared to controls (25(OH)D3<10 ng/mL in 25% versus 12%, p<0.00001), which was in part reversible after HCV eradication. 25(OH)D3 deficiency correlated with SVR in HCV genotype 2 and 3 patients (63% and 83% SVR for patients with and without severe vitamin D deficiency, respectively, p<0.001). In addition, the CYPB27-1260 promoter polymorphism rs10877012 had substantial impact on 1-25- dihydroxyvitamin D serum levels and SVR rates in HCV genotype 1, 2 and 3 infected patients. CONCLUSIONS: Chronic hepatitis C virus infection is associated with vitamin D deficiency. Reduced 25- hydroxyvitamin D levels and CYPB27-1260 promoter polymorphism are associated with failure to achieve SVR in HCV genotype 1, 2, 3 infected patients.

Discovery and fine mapping of serum protein loci through transethnic meta-analysis.

Many disorders are associated with altered serum protein concentrations, including malnutrition, cancer, and cardiovascular, kidney, and inflammatory diseases. Although these protein concentrations are highly heritable, relatively little is known about their underlying genetic determinants. Through transethnic meta-analysis of European-ancestry and Japanese genome-wide association studies, we identified six loci at genome-wide significance (p < 5 × 10(-8)) for serum albumin (HPN-SCN1B, GCKR-FNDC4, SERPINF2-WDR81, TNFRSF11A-ZCCHC2, FRMD5-WDR76, and RPS11-FCGRT, in up to 53,190 European-ancestry and 9,380 Japanese individuals) and three loci for total protein (TNFRS13B, 6q21.3, and ELL2, in up to 25,539 European-ancestry and 10,168 Japanese individuals). We observed little evidence of heterogeneity in allelic effects at these loci between groups of European and Japanese ancestry but obtained substantial improvements in the resolution of fine mapping of potential causal variants by leveraging transethnic differences in the distribution of linkage disequilibrium. We demonstrated a functional role for the most strongly associated serum albumin locus, HPN, for which Hpn knockout mice manifest low plasma albumin concentrations. Other loci associated with serum albumin harbor genes related to ribosome function, protein translation, and proteasomal degradation, whereas those associated with serum total protein include genes related to immune function. Our results highlight the advantages of transethnic meta-analysis for the discovery and fine mapping of complex trait loci and have provided initial insights into the underlying genetic architecture of serum protein concentrations and their association with human disease.

New gene functions in megakaryopoiesis and platelet formation.

Platelets are the second most abundant cell type in blood and are essential for maintaining haemostasis. Their count and volume are tightly controlled within narrow physiological ranges, but there is only limited understanding of the molecular processes controlling both traits. Here we carried out a high-powered meta-analysis of genome-wide association studies (GWAS) in up to 66,867 individuals of European ancestry, followed by extensive biological and functional assessment. We identified 68 genomic loci reliably associated with platelet count and volume mapping to established and putative novel regulators of megakaryopoiesis and platelet formation. These genes show megakaryocyte-specific gene expression patterns and extensive network connectivity. Using gene silencing in Danio rerio and Drosophila melanogaster, we identified 11 of the genes as novel regulators of blood cell formation. Taken together, our findings advance understanding of novel gene functions controlling fate-determining events during megakaryopoiesis and platelet formation, providing a new example of successful translation of GWAS to function.

Meta-analysis identifies multiple loci associated with kidney function-related traits in east Asian populations.

Chronic kidney disease (CKD), impairment of kidney function, is a serious public health problem, and the assessment of genetic factors influencing kidney function has substantial clinical relevance. Here, we report a meta-analysis of genome-wide association studies for kidney function-related traits, including 71,149 east Asian individuals from 18 studies in 11 population-, hospital- or family-based cohorts, conducted as part of the Asian Genetic Epidemiology Network (AGEN). Our meta-analysis identified 17 loci newly associated with kidney function-related traits, including the concentrations of blood urea nitrogen, uric acid and serum creatinine and estimated glomerular filtration rate based on serum creatinine levels (eGFRcrea) (P < 5.0 × 10(-8)). We further examined these loci with in silico replication in individuals of European ancestry from the KidneyGen, CKDGen and GUGC consortia, including a combined total of ∼110,347 individuals. We identify pleiotropic associations among these loci with kidney function-related traits and risk of CKD. These findings provide new insights into the genetics of kidney function.

Mammalian Target of Rapamycin Complex 2 Controls CD8 T Cell Memory Differentiation in a Foxo1-Dependent Manner.

Upon infection, antigen-specific naive CD8 T cells are activated and differentiate into short-lived effector cells (SLECs) and memory precursor cells (MPECs). The underlying signaling pathways remain largely unresolved. We show that Rictor, the core component of mammalian target of rapamycin complex 2 (mTORC2), regulates SLEC and MPEC commitment. Rictor deficiency favors memory formation and increases IL-2 secretion capacity without dampening effector functions. Moreover, mTORC2-deficient memory T cells mount more potent recall responses. Enhanced memory formation in the absence of mTORC2 was associated with Eomes and Tcf-1 upregulation, repression of T-bet, enhanced mitochondrial spare respiratory capacity, and fatty acid oxidation. This transcriptional and metabolic reprogramming is mainly driven by nuclear stabilization of Foxo1. Silencing of Foxo1 reversed the increased MPEC differentiation and IL-2 production and led to an impaired recall response of Rictor KO memory T cells. Therefore, mTORC2 is a critical regulator of CD8 T cell differentiation and may be an important target for immunotherapy interventions.

Espagnol 318

Contient : 1 Bref du pape Jules II aux Rois Catholiques Ferdinand et Isabelle. Rome, 25 mai 1504. En latin ; 2 Bref du pape Alexandre VI aux Rois Catholiques Ferdinand et Isabelle. Rome, 2 octobre 1493. En latin ; 3 Lettre de l'empereur Maximilien Ier aux Rois Catholiques Ferdinand et Isabelle. Strasbourg, 15 avril 1499. En latin ; 4 Lettre de l'empereur Maximilien Ier aux Rois Catholiques Ferdinand et Isabelle. Hall, 17 mars 1496. En français ; 5 Lettre de Frédéric III, roi de Naples, aux Rois Catholiques Ferdinand et Isabelle. San Germano, 11 janvier 1497. En italien et en partie en chiffre ; 6 Lettre de Jeanne de Naples, "la tryste rreyna," veuve de Ferdinand Ier, à son frère Ferdinand le Catholique. Naples, 17 novembre 1508 ; 7 Lettre de Jeanne de Naples, "la tryste rreyna," veuve de Ferdinand Ier à son père Jean II d'Aragon. Naples, 8 octobre 1477. En catalan ; 8 Lettre de Jeanne de Naples, "la tryste rreyna," veuve de Ferdinand Ier à son frère Ferdinand le Catholique. Castellamar (?), 23 juillet 1509 ; 9 Lettre des Rois Catholiques à la comtesse de la Cherra. Alcalá de Henares, 20 janvier 1503 ; 10 Lettre des Rois Catholiques à Mossen de Rebestan. Alcalá de Henares, 5 avril 1498 ; 11 Lettre des Rois Catholiques à l'empereur Maximilien. 1503. Duplicata ; 12 Lettre de Ferdinand le Catholique à son père Jean II d'Aragon. Séville, 25 juillet 1478 ; 13 Lettre des Rois Catholiques à N... "duque primo," lui défendant d'intervenir les armes à la main dans les affaires de la maison de Medinaceli, sous peine de perdre ses biens. Écija, décembre 1501. Minute ; 14 Lettres de créance des Rois Catholiques pour Gonçalo Fernandez de Cordova. Grenade, 22 mars 1501 ; 15 Lettre d'"Ysabella de Aragonia, duchesa de Millano," au Roi Catholique. Bari, 14 décembre 1507. En italien ; 16 Lettre de "Leonor," princesse de Navarre, au roi Jean II, son père. Olit, 10 décembre 1473 ; 17 Lettre de "Janus Maria de Campo Fregoso," doge de Gênes, au Roi Catholique. Gênes, 6 juillet 1512. En italien ; 18 Lettre d'Alphonse d'Est, duc de Ferrare, au Roi Catholique. Ferrare, 25 janvier 1507. En italien ; 19 Lettre de "Johan" d'Albret et "Catalina," rois de Navarre, au Roi Catholique. Pau, 9 octobre 1510 ; 20 Lettre d'"el prinçipe" D. Carlos au Roi Catholique. Malines, 26 octobre 1508 ; 21 Lettre d'"Anne," duchesse de Bretagne, à l'ambassadeur des Rois Catholiques, Francisco de Rojas. Rennes, 18 avril 1490. En français ; 22 Lettre d'"Anne," duchesse de Bretagne, reine de France, au Roi Catholique. Moulins, 24 mai 1497. En français ; 23 Lettre de Ferdinand le Catholique à son père Jean II. Sans lieu ni date ; 24 Lettre de "el Rey M[anuel]" de Portugal au Roi Catholique. Allmeirim, 7 mars 1510. En portugais ; 25 Lettre d'"Anne" de France au Roi Catholique. Paris, 30 juillet 1513. En français ; 26 Lettre de "Pierre" II de Bourbon à la Reine Catholique. Villefranche en Beaujolais, 12 décembre. En français ; 27 Lettre d'Alphonse II, roi de Naples, au Roi Catholique. Messine, 11 mai 1495. En italien ; 28 Lettre de Ferdinand Ier, roi de Naples, à son oncle Jean II d'Aragon. Naples, 3 mai 1473. En italien ; 29 Lettre de "Don Fadryque" III, roi de Naples, à la Reine Catholique. "De my rreal sobre Gaeta," 21 novembre 1496 ; 30 Lettre de "la infelicissima regina Ysabella," femme de Frédéric III de Naples, au Roi Catholique. "In lo placis de Turs." (Tours) 10 janvier. En italien ; 31 Lettre de Louis XII, roi de France, au Roi Catholique. 30 mars 1508. En français ; 32 Lettre de Charles VIII, roi de France, au Roi Catholique. Amboyse, 26 septembre. En français ; 33 Lettre de Louis XII au Roi Catholique. Rouenne, 26 mars 1509. En français ; 34 Lettre de Louis XII au Roi Catholique. Bloys, 14 septembre 1505. En français ; 35 Lettre de "la princesa de Gales," Catherine d'Aragon, au Roi Catholique. Rryxamon (Richmond), 29 mai ; 36 Lettre de Gonzalo Fernandez de Cordova, "duque de Terranova," à Miguel Perez de Almazan. Sans date ; 37 Lettre de "Juan Manuel" aux Rois Catholiques. Gênes, 8 janvier 1497 ; 38 Lettre de "Gaston" de Foix au Roi Catholique. Sans date. En français ; 39 Lettre de "la princesa de Gales," Catherine d'Aragon, au Roi Catholique. Granuche (Greenwich), 21 novembre 1508 ; 40 Lettre de Béatrix de Naples, "la infelicissima regina de Hungaria et Bohemia," fille de Ferdinand Ier de Naples et femme de Ladislas VI, roi de Hongrie, au Roi Catholique. Naples, 2 mars 1508. En italien ; 41 Lettre de Louis XII, roi de France, à l'archiduc Philippe. Loches, 24 novembre 1502. En français ; 42 Lettre d'Arthur, prince de Galles, à Catherine d'Aragon, sa femme. "Ex manerio Woodstoke," 9 septembre 1497. En latin ; 43 Lettre d'"el Rey M[anuel]" de Portugal au Roi Catholique. Evora, 22 décembre 1512. En portugais ; 44 Lettre de Marie, reine de Portugal, au Roi Catholique. Evora, 2 décembre 1508. En portugais ; 45 Lettre de Henri VII, roi d'Angleterre, aux Rois Catholiques. Greenwich, 15 mai 1497. En latin ; 46 Lettre de Jacques IV, roi d'Écosse, aux Rois Catholiques. Édimbourg, 27 octobre 1497. En latin ; 47 Lettre d'Élisabeth, reine d'Angleterre, à la Reine Catholique. Greenwich, 11 mars 1498. En latin ; 48 Lettre de l'archiduc Philippe et de Marguerite d'Autriche aux Rois Catholiques. Bruxelles, 16 décembre 1495. En français ; 49 Lettre de Catherine, reine de Navarre, au Roi Catholique. Pampelune, 12 juin 1512 ; 50 Lettre d'Isabelle, reine de Castille, au roi Jean II d'Aragon, son beau-père. Madrigal, 30 avril ; 51 Lettre de Madeleine de France, régente de Navarre, aux Rois Catholiques. Pampelune, 16 novembre 1494. En français ; 52 Lettre de la Reine Catholique aux maîtres et pilotes de la flotte qui doit conduire en Angleterre l'infante Catherine. Grenade, septembre 1501. Duplicata ; 53 Lettre du Roi Catholique au gouverneur du "principato Citra." Naples, 30 mai 1507. En italien ; 54 Lettre d'Isabelle, reine de Portugal, à Miguel Perez de Almazan, secrétaire des Rois Catholiques. Yelves, 5 avril 1498 ; 55 Lettre de Ferdinand II, roi de Naples, à la Reine Catholique. Procita, dernier jour de février. En italien ; 56 Lettre d'un agent du Roi Catholique à "Mosen Coloma," datée : "En el real de Valençya." ; 57 Lettre de "La Mouche de Veyre" à la Reine Catholique. Lyon, 23 juillet. En français ; 58 Lettre de Bernardino de Lezcano au Roi Catholique. Tolosa, 23 novembre 1512 ; 59 Lettre d'Yves II, baron d'Alègre, à Yñigo Lopez. Troye, 27 février 1502. En italien ; 60 Billet de Jean-Jacques Trivulci à un secrétaire des Rois Catholiques. Bles (Blois ?), 29 novembre 1513. En italien ; 61 Lettre de D. Iñigo Davalos y Aquino, marquis del Vasto, aux Rois Catholiques. Castello de Iscla, 26 avril 1503 ; 62 Lettre de Gonzalo Fernandez de Cordova au Roi Catholique. "Del real sobre Gaeta," 17 septembre 1503 ; 63 Lettre d'Antonio de Leyva au Roi Catholique. Naples, 6 mai 1512 ; 64 Lettre de Béatrix de Naples au Roi Catholique. Naples, 30 octobre 1507. En italien ; 65 Lettre de Jeanne de Naples, "la tryste reyna," veuve de Ferdinand II, à sa tante la Reine Catholique. Sans date ; 66 Lettre de Marie, reine de Portugal, à son père le Roi Catholique. Almeryn, 26 mars 1508 ; 67 Lettre de Henri VIII, roi d'Angleterre, au Roi Catholique. Windsor, 25 septembre 1511. En latin ; 68 Lettre de Marguerite d'Autriche au Roi Catholique. Bruxelles, 25 novembre 1509 ; 69 Lettre de Marguerite d'York, duchesse douairière de Bourgogne, à la Reine Catholique. Audermunde, 25 août 1493. En latin ; 70 Lettre d'Anne, reine de France, aux Rois Catholiques. Loches, 31 janvier. En français ; 71 Lettre de Philippe, archiduc d'Autriche, à sa belle-soeur Catherine, princesse de Galles. Bruxelles, 8 août 1498. En français ; 72 Lettre de Fabritio Colonna au Roi Catholique. Naples, 23 octobre 1511. En italien ; 73 Lettre de Bartholomeo d'Alviano au Roi Catholique. Fréjus, 2 avril 1508. En italien ; 74 Lettre de Gui de Blanchefort, grand maître de Rhodes, au Roi Catholique. Lyon, 24 juin 1513. En latin ; 75 Lettre d'Agostino Adorno, gouverneur du duché de Gênes, à Antonio de Grimaldis. Gênes, 7 juillet 1496. En italien ; 76 Mémoire du duc de Milan, Ludovico Maria Sforza, pour son ambassadeur à Venise, Octaviano Vicomercato. Milan, 4 avril 1496. En italien ; 77 Lettre de Gonzalo Fernandez de Cordova au Roi Catholique. 29 novembre ; 78 Lettre de Gonzalo Fernandez de Cordova au Roi Catholique. Naples, 15 octobre 1505 ; 79 Lettre de Gonzalo Fernandez de Cordova à Miguel Perez de Almazan, secrétaire des Rois Catholiques. Naples, 16 février 1504 ; 80 Lettre de Gonzalo Fernandez de Cordova à Miguel Perez de Almazan, secrétaire des Rois Catholiques. Naples, 12 novembre 1505 ; 81 Lettre de Gonzalo Fernandez de Cordova aux Rois Catholiques. Rome, 28 mars 1497 ; 82 Lettre du cardinal Jean Colonna au Roi Catholique. Rome, 14 février 1506. En italien ; 83 Lettre de recommandation de Juan de Ribera, archevêque de Valence et patriarche d'Antioche, pour Juan de Borja, fils de Francisco de Borja. Valence, 27 novembre 1570 ; 84 Lettre de Lorenzo Pucci, cardinal des Quatre-Saints, au Roi Catholique. Rome, 12 juillet 1514. En italien ; 85 Lettre de Gonzalo Fernandez de Cordova au Roi Catholique. Madrid (?), 7 août 1510 ; 86 Lettre de Fernando de Alarcon au Roi Catholique. Naples, 18 mai 1508 ; 87 Lettre de Christoval Camudio à Miguel Perez de Almazan. Osma, 25 mars ; 88 Lettre de Pedro Navarro au Miguel Perez de Almazan. Burgos, 29 septembre 1507 ; 89 Lettre de Prospero Colonna à Gonzalo Fernandez de Cordova. Rome, 19 août 1505. En italien ; 90 Lettre d'Ugo de Moncada au Roi Catholique. Cosenza, 22 janvier ; 91 Lettre de Giovane Caraccioli, prince de Melfi, au Roi Catholique. Barletta, 17 juin 1509. En italien ; 92 Lettre, en partie chiffrée, de Gonzalo Fernandez de Cordova à Lorenzo Suarez, 17 août 1500. Copie remise à Miguel Perez de Almazan ; 93 Lettre en chiffre de Lorenzo Suarez aux Rois Catholiques. Venise, 24 février 1504 ; 94 Lettre en chiffre du vice-roi de Sicile au Roi Catholique. Messine, 27 avril 1503 ; 95 Chiffre sans adresse, daté du 8 janvier 1497 ; 96 Lettre du cardinal Luis de Borja à Miguel Perez de Almazan. Naples, 17 avril 1510 ; 97 Lettre de Pierre II de Bourbon, seigneur de Beaujollais, à M. de Maure. Moulins, 14 août. En français ; 98 Lettre de Cifontes à la Reine Catholique. Ramua, 29 octobre 1496 ; 99 Lettre de Gonzalo Fernandez de Cordova à Miguel Perez de Almazan. Naples, 25 août 1505 ; 100 Lettre de Yahya ben Sa'id ben Meymoun à Douan Ramoun el-Kardoun (Ramon de Cardona),... chef de Naples. Mars 1511. En arabe