Per qu?? s??c, penso i actuo aix?? (pedag??gicament)? : influ??ncies que han marcat un cam?? cap a la identitat professional

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Hist??ria de vida : itinerari personal i professional d???un cam?? recorregut amb entusiasme

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Escoles creatives, un cam?? per a l'??xit

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L'experi??ncia d'obrir cam??

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El frac??s escolar : un cam?? llarg i desigual

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La cohesi?? d???equips docents, un cam?? per aprendre

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Un Camí del SAD al SED

L’objectiu principal del treball és exposar una alternativa a la institucionalització que fomenti l’autonomia, el desenvolupament i el benestar de les persones que es troben en situació de dependència, partint d’un recurs ja existent: el Servei d’Atenció Domiciliària (SAD). Va dirigit en especial al col•lectiu de persones amb problemàtiques de salut mental. Proposa la nomenclatura SED (Suport Educatiu Domiciliari) què no existeix com a tal, per anomenar una branca o una part del SAD que sí existeix

El Camí de Sant Jaume en la provincia de Girona

En primer lugar se identifican las distintas rutas del Camí de Sant Jaume en la provincia de Girona con el objetivo de determinar cuáles han sido las principales rutas que los peregrinos han utilizado para su peregrinación hacia Santiago de Compostela dentro de la provincia de Girona. En segundo lugar se establecen los elementos que determinan que un itinerario concreto esté comprendido en el Camino de Santiago para lo cual se analizan las rutas y se buscan los componentes que tienen una relación directa con la peregrinación a Santiago de Compostela, bien por ser elementos descriptivos del Santo, bien por ser elementos que a lo largo de la historia se han atribuido a los peregrinos que peregrina a Santiago de Compostela. En tercer lugar se analiza el recorrido del Camí de Sant Jaume elegido por la Generalitat de Catalunya, se examinan los servicios que ofrece y las carencias o insuficiencias que posee. Finalmente se establece la confección de propuestas de mejora y las acciones pertinentes para corregir las debilidades que en su caso puedan tener estos itinerarios

Modeling chemotaxis reveals the role of reversed phosphotransfer and a bi-functional kinase-phosphatase

Understanding how multiple signals are integrated in living cells to produce a balanced response is a major challenge in biology. Two-component signal transduction pathways, such as bacterial chemotaxis, comprise histidine protein kinases (HPKs) and response regulators (RRs). These are used to sense and respond to changes in the environment. Rhodobacter sphaeroides has a complex chemosensory network with two signaling clusters, each containing a HPK, CheA. Here we demonstrate, using a mathematical model, how the outputs of the two signaling clusters may be integrated. We use our mathematical model supported by experimental data to predict that: (1) the main RR controlling flagellar rotation, CheY6, aided by its specific phosphatase, the bifunctional kinase CheA3, acts as a phosphate sink for the other RRs; and (2) a phosphorelay pathway involving CheB2 connects the cytoplasmic cluster kinase CheA3 with the polar localised kinase CheA2, and allows CheA3-P to phosphorylate non-cognate chemotaxis RRs. These two mechanisms enable the bifunctional kinase/phosphatase activity of CheA3 to integrate and tune the sensory output of each signaling cluster to produce a balanced response. The signal integration mechanisms identified here may be widely used by other bacteria, since like R. sphaeroides, over 50% of chemotactic bacteria have multiple cheA homologues and need to integrate signals from different sources.

Phosphoinositide (PI) 3-kinase assays

The regulation of phosphoinositide (PI) 3-kinase activities has been linked to many normal and disease-related processes, including cell survival, cell growth and proliferation, cell differentiation, cell motility, and intracellular vesicle trafficking. However, as the family of enzymes has now grown to include eight true members, in three functional classes, plus several related protein kinases that are also inhibited by the widely used PI 3-kinase selective inhibitors, wortmannin and LY294002, extended methodologies are required to identify which type of kinase is involved in a particular cellular process, or protein complex, under study. A robust in vitro PI 3-kinase assay, suitable for use with immunoprecipitates, or purified proteins, is described here together with a series of modifications of substrate and assay conditions that will aid researchers in the identification of the particular class and isoform of PI 3-kinase that is involved in a signaling process under investigation.

Hepatitis C virus NS5A protein binds the SH3 domain of the Fyn tyrosine kinase with high affinity: mutagenic analysis of residues within the SH3 domain that contribute to the interaction

Background: The hepatitis C virus (HCV) non-structural 5A protein (NS5A) contains a highly conserved C-terminal polyproline motif with the consensus sequence Pro-X-X- Pro-X-Arg that is able to interact with the Src-homology 3 (SH3) domains of a variety of cellular proteins. Results: To understand this interaction in more detail we have expressed two N-terminally truncated forms of NS5A in E. coli and examined their interactions with the SH3 domain of the Src-family tyrosine kinase, Fyn. Surface plasmon resonance analysis revealed that NS5A binds to the Fyn SH3 domain with what can be considered a high affinity SH3 domain-ligand interaction (629 nM), and this binding did not require the presence of domain I of NS5A (amino acid residues 32-250). Mutagenic analysis of the Fyn SH3 domain demonstrated the requirement for an acidic cluster at the C-terminus of the RT-Src loop of the SH3 domain, as well as several highly conserved residues previously shown to participate in SH3 domain peptide binding. Conclusion: We conclude that the NS5A: Fyn SH3 domain interaction occurs via a canonical SH3 domain binding site and the high affinity of the interaction suggests that NS5A would be able to compete with cognate Fyn ligands within the infected cell.

A dual role for integrin-linked kinase in platelets: regulating integrin function and alpha-granule secretion

Integrin-linked kinase (ILK) has been implicated in the regulation of a range of fundamental biological processes such as cell survival, growth, differentiation, and adhesion. In platelets ILK associates with beta 1- and beta 3-containing integrins, which are of paramount importance for the function of platelets. Upon stimulation of platelets this association with the integrins is increased and ILK kinase activity is up-regulated, suggesting that ILK may be important for the coordination of platelet responses. In this study a conditional knockout mouse model was developed to examine the role of ILK in platelets. The ILK-deficient mice showed an increased bleeding time and volume, and despite normal ultrastructure the function of ILK-deficient platelets was decreased significantly. This included reduced aggregation, fibrinogen binding, and thrombus formation under arterial flow conditions. Furthermore, although early collagen stimulated signaling such as PLC gamma 2 phosphorylation and calcium mobilization were unaffected in ILK-deficient platelets, a selective defect in alpha-granule, but not dense-granule, secretion was observed. These results indicate that as well as involvement in the control of integrin affinity, ILK is required for alpha-granule secretion and therefore may play a central role in the regulation of platelet function. (Blood. 2008; 112: 4523-4531)