Modelagem do crescimento e produção aplicado ao manejo florestal na Amazônia brasileira

A floresta Amazônica possui um papel ambiental, social e econômico importante para a região, para o país e para o mundo. Dessa forma, técnicas de exploração que visam a diminuição dos impactos causados à floresta são essenciais. Com isso, o objetivo dessa tese é comparar a Exploração de Impacto Reduzido com a Exploração Convencional na Amazônia brasileira através de modelos empíricos de árvore individual de crescimento e produção. O experimento foi instalado na fazenda Agrossete, localizada em Paragominas - PA. Em 1993, três áreas dessa fazenda foram selecionadas para exploração. Na primeira área, 105 hectares foram explorados através da Exploração de Impacto Reduzido. Na segunda área, 75 hectares foram submetidos à Exploração Convencional. E, por fim, a terceira área foi mantida como área testemunha. A coleta de dados de diâmetro à altura do peito e a identificação das espécies dentro de uma parcela de 24,5 hectares, instalada aleatoriamente em cada área, foi realizada nos anos de 1993 (antes da colheita), 1994 (seis meses depois da colheita), 1995, 1996, 1998, 2000, 2003, 2006 e 2009. Dessa forma, as três áreas foram comparadas através do ajuste de um modelo de incremento diamétrico, considerando que efeito estocástico podia assumir outras quatro distribuições além da distribuição normal, de um modelo de probabilidade de mortalidade e de um modelo de probabilidade de recrutamento. O comportamento do incremento diamétrico indicou que as áreas que foram submetidas a exploração possuem o mesmo comportamento em quase todos os grupos de espécies, com exceção do grupo de espécies intermediárias. Os indivíduos que são submetidos a exploração possuem um maior crescimento em diâmetros quando comparados com área que não sofreu exploração. Além disso, assumir o efeito estocástico com distribuição Weibull melhorou o ajuste dos modelos. Em relação à probabilidade de mortalidade, novamente as áreas que sofreram exploração possuem comportamento semelhante quanto à mortalidade, mas diferente da área que não foi explorada, sendo que os indivíduos localizados nas áreas exploradas possuem uma maior probabilidade de morte em relação aos presentes na área não explorada. Os modelos de probabilidade de recrutamento indicaram diferença apenas entre as áreas exploradas e a área controle. Sendo que, as áreas exploradas apresentaram uma maior taxa de recrumento em comparação a área não explorada. Portanto, o comportamento individual das árvores após a exploração é o mesmo na Exploração Convencional e na Exploração de Impacto Reduzido.

Effects of ocean warming and acidification on the early stages of marine fishes

Tese de doutoramento, Ciências do Mar, Universidade de Lisboa, Faculdade de Ciências, 2016

As TIC na inclusão de alunos com NEE no ensino das ciências

Este estudo debruça-se sobre as atitudes dos professores de ciências face à adoção das Tecnologias da Informação e Comunicação na sua prática pedagógica com alunos com Necessidades Educativas Especiais e de que forma essa prática contribui para a inclusão efetiva destes alunos. De forma a encontrar respostas esclarecedoras face ao tema proposto, foi levada a cabo uma revisão de literatura que visou clarificar os conceitos relacionados com o mesmo: as Tecnologias da Informação e Comunicação (TIC), a inclusão de alunos com Necessidades Educativas Especiais (NEE) e o ensino das Ciências. Sobre estes conceitos, foram focados os seguintes itens: como evoluíram; qual a sua importância e como se relacionam. Procedemos à elaboração de um inquérito por questionário com o intuito de recolher a informação necessária para responder aos objetivos do estudo. Este foi aplicado a professores de Ciências (grupos de recrutamento 500,510, 520 e 550) do ensino básico e secundário, cujas respostas constituem a amostra do estudo. O estudo dos resultados obtidos através da aplicação do questionário procura esclarecer a seguinte questão central: Qual o papel das TIC na inclusão de alunos com NEE no ensino das Ciências? Após o tratamento e análise de dados foram tiradas as respetivas conclusões das quais fazem parte a atitude positiva dos professores de Ciências face à inclusão de alunos com Necessidades Educativas Especiais (NEE) no Ensino Regular e o reconhecimento da importância da utilização das Tecnologias da Informação e Comunicação (TIC) nesse processo.

O IMPACTO DAS POLÍTICAS E PRÁTICAS DE RECURSOS HUMANOS SOBRE OS COMPORTAMENTOS DE CIDADANIA ORGANIZACIONAL

O objetivo deste estudo é investigar se as políticas e práticas de recursos humanos (PPRH) impactam os comportamentos de cidadania organizacional (CCO), a fim de proporcionar aos gestores uma compreensão de como os indivíduos, tão diferentes entre si, comportam-se dentro e fora da organização diante daquilo que lhes é estabelecido pela área de recursos humanos. No campo teórico, foram consideradas as obras de Milkovich e Boudreau (1994), Dessler (2002), Demo et al. (2012) entre outros para abordar a questão de PPRH e os trabalhos de Handy (1978), Organ (1990), Siqueira (2003) entre outros para a temática CCO. A metodologia utilizada nesse estudo trata-se de uma pesquisa quantitativa entre 156 trabalhadores de empresas públicas, privadas e mistas localizadas no Estado de São Paulo, sendo que para a captação dos dados foram aplicados questionários de autopreenchimento, composto por duas escalas, sendo uma voltada a PPRH (DEMO et al., 2012) e outra cujas questões são relacionadas a CCO (BASTOS et al., 2014). Como resultados, identificou-se que PPRH impacta de forma significativa CCO e que a política de envolvimento, que trata da criação de um vínculo afetivo entre a organização e seus colaboradores, contribuindo para o seu bem-estar em termos de reconhecimento, relacionamento, participação e comunicação (Demo et al., 2012, p.38), é a única que impacta de forma significativa CCO, se comparados aos impactos causados pelas políticas de recrutamento e seleção, treinamento e desenvolvimento, condições de trabalho, avaliação de desempenho e remuneração e recompensas. Conclui-se também que o conjunto de PPRH impacta com maior significância os fatores de CCO denominados divulgação da imagem organizacional e sugestões criativas. Não houve impacto significativo entre PPRH e o fator cooperação com os colegas.

DEMANDA DE MÃO DE OBRA DA PETROBRAS POR NÍVEIS DE COMPETÊNCIAS A PARTIR DA DESCOBERTA DO PRÉ-SAL

A pesquisa possui como objetivo geral levantar, analisar, quantificar e classificar por níveis de competências quais foram os profissionais recrutados pela Petrobras no período pós-descoberta da camada do pré-sal brasileiro. A pesquisa se justifica pela previsão de crescimento da produção nacional de petróleo e gás natural estimada para os próximos anos o que poderá causar um descompasso entre a oferta e a demanda de mão de obra para o seu desenvolvimento. A abordagem metodológica desenvolvida para realização da pesquisa foi a da pesquisa exploratória, descritiva e documental, através de análise qualitativa e quantitativa longitudinal. Como resultado, a pesquisa revelou que a Petrobras não recruta profissionais para posições de nível gerencial. Os resultados demonstraram ainda que 56,8% das vagas abertas ao recrutamento são destinadas aos profissionais com formação de nível médio e que 76,4porcento das vagas são relacionadas ao processo fabril evidenciando que a Petrobras utiliza como porta de entrada a contratação de profissionais de nível médio com formação técnica. Ao realizar a classificação e qualificação da oferta de vagas abertas ao recrutamento a pesquisa identificou cinco grupos de profissionais distribuídos por três eixos de carreira e quatro níveis salariais que quando categorizados por níveis de competências que foram responsáveis por 69porcento de todas as vagas abertas ao recrutamento. Os dois primeiros grupos em relevância estão relacionados ao eixo de carreira de operações industriais onde o nível superior (O6) e o nível inferior (O1) foram os responsáveis por 22porcento e 21porcento respectivamente do total da oferta de vagas no período. O terceiro grupo em importância diz respeito ao eixo de carreira engenharia, processos e projetos onde os profissionais categorizados com nível médio (E3) numa escala de dois a cinco foram os responsáveis por 13porcento do total de vagas abertas. O quarto e quinto grupos estão relacionados ao eixo de carreira gestão de negócios e categorizados por níveis de competências nos níveis três (G3) e quatro (G4) em uma escala de um a cinco sendo estes responsáveis 7porcento e 6porcento do total de vagas.

Trajetos de exclusão e reclusão: oral temática de familiares atingidos pelo tratamento asilar da hanseníase

A história da hanseníase é marcada por preconceito, exclusão social, estigma, abandono e medo, por ter sido conhecida durante muito tempo como incurável e contagiosa. Além dos agravos inerentes às alterações dermatoneurológicas e consequentes incapacidades físicas, são ressaltadas as repercussão emocionais, alterações nos hábitos cotidianos e mudanças na configuração familiar. Atualmente a hanseníase é conhecida como doença negligenciada, com alta incidência e prevalência, considerada como um problema de saúde alvo de incentivos e mobilizações das políticas públicas. Ao estudar a história da hanseníase, parte-se do pressuposto de que pouco se sabe sobre as repercussões da doença do passado na vida dos familiares de ex-doentes tratados em regime asilar, assim como a visão e os sentimentos dos mesmos familiares diante da hanseníase na atualidade. Portanto, objetivou-se narrar a história de familiares de ex-doentes de hanseníase que foram tratados em hospital colônia. Os objetivos específicos são: Identificar se familiares de pacientes com hanseníase tratados em hospitais colônia eram atingidos pelo preconceito, estigma e exclusão que permeava a vida dos portadores da doença; Verificar se o tratamento de ex-doentes de hanseníase em hospitais colônia alterou a efetivação de laços familiares tais indivíduos e os membros de sua família; Averiguar qual a compreensão que familiares de ex-doentes de hanseníase tratados em hospitais colônia têm sobre a hanseníase; Promover, junto aos participantes da pesquisa, atividade de promoção da saúde sobre hanseníase. Adotou-se o estudo exploratório e descritivo, com abordagem qualitativa com suporte na História Oral de Temática como técnica e referencial metodológico. Os 52 familiares de ex-doentes de lepra que foram segregados no Hospital Colônia São Francisco de Assis, cadastrados no MORHAN-Potiguar, constituíram a colônia. A partir do ponto zero houve o recrutamento dos participantes que compuseram a rede, totalizando 10 colaboradores, de ambos os sexos e idade de 44 a 76 anos. Após aprovação pelo Comitê de Ética em Pesquisa - UFRN, sob o protocolo 650.654/2014 e CAAE 25922214.3.0000.5537, realizou-se a coleta de dados por meio de entrevista, utilizando instrumento de identificação da rede e questões abertas. As entrevistas foram gravadas, transcritas, conferidas pelos colaboradores e posteriormente transcriadas. Tratou-se as histórias, narradas pela técnica de Análise Temática de Conteúdo, segundo Bardin, emergindo três eixos temáticos: Impacto nas relações sociais (Estigma e preconceito; Exclusão social); Impacto nas relações familiares (Desagregação familiar; Restrições para visita; Compartilhamento e construção de uma nova família; Consequências familiar geradas pelo isolamento; Reconstrução do vínculo familiar); e Pensamentos frente a lepra e a hanseníase (A história no passado; A história no presente). O fato de ter um familiar doente de hanseníase segregado em hospital colônia gerou empecilhos nas relações sociais vivenciadas pelos colaboradores do estudo, que embora não tivessem a doença, foram vitimados pela exclusão social, estigma e preconceito. O internamento compulsório também gerou modificações na estrutura familiar, com distanciamento, alteração no vínculo e tentativa de reestruturação familiar. Os colaboradores também refletiram sobre política de controle da lepra no passado, assim como a adotada no presente frente à hanseníase.

História oral de vida de pessoas com úlcera venosa nos serviços de atenção primária à saúde

The Chronic Venous insufficiency is characterized as a set of physical changes including how most serious complication of venous ulcers, characterized by irregular and progressive loss of continuity of the skin. The occurrence of venous ulcers in people with chronic venous insufficiency generates dependence on them with health services, with long-term treatments that cause limitations and high-impact changes, affecting their quality of life, affecting the physical, psychological, social, cultural and spiritual as an important public health problem. This study aimed to describe the experience of having a venous ulcer, in the scenario of primary health care services to Health, which includes Primary Care Units and Family Health Strategy in the city of Natal / RN, based on the life histories of users. This is a qualitative study, exploratory and descriptive, with the Oral History of Life as a methodological framework. From the ponto zero was the recruitment of participants who formed the network, totaling six employees, of both sexes and aged between 57 and 79 years. After approval by the Research Ethics Committee - UFRN under the Protocol 653 788/2014 and CAAE 30408014.0.0000.5537 was held data collection, between the months of July and August, through interviews, using identification and characterization of the instrument employees and open questions. Interviews were recorded, transcribed, transcriadas and returned to employees for a conference. The narratives were subjected to Content thematic analysis technique, according to Bardin, allowing the construction of three themes that encompass categories, namely: Axis I - Perspectives on the changes: the impact wound in social relations (changes with ulcer venous, venous ulcer and social and family relationships); Axis II - Brands in body and soul: the story of being hurt (conceptions of the body injured; therapeutic itinerary in primary care services); and Axis III - Reconstruction of being hurt: coping mechanisms (redefinition of the wounded body, resilience to chronic wound). The impact of having a chronic venous ulcer generates impact of physical, psychological and social order. As aspects related to changes after the appearance of venous ulcers, survey participants reported the presence of pain, physical limitations, psychological distress, social and emotional isolation, incapacity, aesthetic discomfort and dependency on health services; the family was the aspect thatshowed no significant change after the occurrence of wound for most participants, an ally in the therapeutic process as a support network. The redefinition of the body and the wound are the main coping mechanism of chronic condition. The services in the Primary Care Network play a fundamental role in the rehabilitation of patients with venous ulcers, although there are difficulties in accessing appropriate treatment and need for expanded services, with permanent professional training of health teams and providing the resources managers to strengthen the comprehensive care of people with venous ulcers in Health Primary Care.

Avaliação do efeito da inibição do reparo de sítios abásicos na resposta inflamatória celular

Base excision repair (BER) proteins has been associated with functions beyond DNA repair. Apurynic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein involved in a plethora of cellular activities, such as redox activation of transcription factors, RNA processing and DNA repair. Some studies have described the action of the protein 8-oxoguanine (OGG1) in correcting oxidized lesions in promoters as a step in the transcription of pro-inflammatory cytokines. Despite being especially important in redox activation of transcription factors such as nuclear factor κB (NF-κB) and AP- 1, the repair activity of APE1 has not yet been associated with the inflammatory response. In this study, experimental and bioinformatic analysis approaches have been used to investigate the relationship between inhibition of the repair of abasic sites in DNA by MX, a synthetic molecule designed to inhibt the repair activity of APE1, and the modulation of the inflammatory response. The results showed that treatment of monocytes with lipopolysaccharide (LPS) and MX reduced the expression of cytokines, chemokines and toll-like receptors, and negatively regulated biological immune processes, as macrophages activation, and NF-κB and tumor necrosis factor (TNF-α) and interferon pathways, without inducing cell death. The transcriptomic analysis suggests that LPS/MX treatment induces mitochondrial dysfunction, endoplasmic reticulum stress and activation of autophagy pathways, probably activated by impairment of cellular energy and/or the accumulation of nuclear and mitochondria DNA damage. Additionally, it is proposed that the repair activity of APE1 is required for transcription of inflammatory genes by interaction with abasic sites at specific promoters and recruitment of transcriptional complexes during inflammatory signaling. This work presents a new perspective on the interactions between the BER activity and the modulation of inflammatory response, and suggests a new activity for APE1 protein as modulator of the immune response in a redox-independent manner.

A instrução paulista à época da Guerra do Paraguai (1864-1870)

This study aimed to analyze the Paraguayan War's impact on public education in the province of São Paulo in the period that lasted the conflict (1864-1870). Between 1864 and 1870 the area now known as South America experienced its greatest armed conflict. Brazil, Argentina and Uruguay declared war against Paraguay. Brazilian governors began to conceive the Empire as superior to the Paraguayan nation, which for them, was a non-civilized country. They understood that to maintain civilization and follow the progress, they needed to develop their education. The Empire mobilized men of any part of its territory. Some left for the war voluntarily and others were forced. Along that current War, the recruitment had become increasingly difficult, leading entire families moving, teachers and students to leave the school and the city. The paulistas teachers have been turning to the patriotic past of the province in which he was proclaimed the independence of the Empire. Civilization and progress, present at the leaders speeches were reflected in the São Paulo social practices that culminate in the notion of patriotism, also reflected in the education of children and youth of the period.

O impacto da remoção de peixes sobre a dinâmica e estrutura dos grupos funcionais fitoplanctônicos em um lago raso tropical durante uma seca severa

The artifi cial eutrophication is one of the biggest t h reat for the quality of aquatic ecosystems in the whole world. The expectations for the future climatic scenarios in arid and semi - arid regions are intense and frequent droughts enhancing the risk of eutrophicati on and cyanobacterial blooms. Restoration techniques of eutrophic lakes were proposed to reduce nutrient loading and improve the water quality. A successful technique used in temperate regions is the biomanipulation by benthivorous fish removal . Our hypoth esis is that the benthivorous fish removal reduces phytoplankton total biomass and change the composition of phytoplankton functional groups, improving water quality. The aim of the study was evaluate the impact of biomanipulation on phytoplankton function al groups and in the water quality. We applied the technique of biomanipulation in the artificial lake ESEC, in a semi - arid region of Brazil and analyzed the physical and chemical variables and the dynamic of phytoplankton functional groups monthly during November 2012 to August 2013. With the removal of benthivorous fish we observed a significant increase of the euphotic depth, phytoplankton richness and the recruitment of green algae (groups F and J ), indicators of good water quality. However, we did not observe significant differences on total phosphorous concentration and on phytoplankton biomass and diversity. The drought effect in the region during the study was evident , promoting a drastic reduction on water level which influenced the availability of resource and affected phytoplankton community before the biomanipulation. To evaluate the effect of severe drought on the dynamic of phytoplankton functional groups and test if the drought periods are favorable to dominance of cyanobacterial groups, we stu died two artificial neighbors lakes (ESEC and Pocinhos) in a semi - arid tropical region during May 2012 to February 2013. We observed a temporal differentiation of biotic and abiotic variables caused by drought. Both lakes presented reduction of 2 meters of water level and increase on conductivity, turbidity, nutrients concentration and a reduction on water transparency, during the severe drought. The deeper lake (Pocinhos) increased phytoplankton total biomass and presented cyanobacterial functional group d ominance (group S N ) and the shallower lake (ESEC) reduced phytoplankton total biomass and presented dominance of mixotrophic and flagellate functional groups (groups W 1 e W 2 ). Summarizing, the knowledge of the effects of benthivorous fish removal in semi - a rid tropical lakes still unknown and this study had limitations caused by the impact of drought. Thus, it is necessary a long term monitoring to investigate the real effects of biomanipulation on the functioning of the studied ecosystems. Otherwise, period s of drought could have opposite effects (increase or reduction) on total biomass and composition of phytoplankton functional groups. Drought not always leads to dominance of cyanobacterial groups.

Efeitos in vivo e in vitro de agonistas parciais do receptor NOP: implicações para o tratamento da ansiedade, depressão e mania

Introduction: This study aimed to investigate the effects of the two peptide NOP partial agonists (UFP-113 and [F/G]N/OFQ(1-13)NH2) and the non peptide NOP partial agonist (AT-090) in the mouse emotional behavior as well as in the intracellular transduction pathways following the receptor binding. Methods: Male Swiss or CD-1 mice were used in this study together with NOP(+/+) and NOP(-/-) mice. The elevated plus maze (EPM) was used to evaluate the effects of compounds on anxiety-like behaviors. Diazepam and the NOP agonists, N/OFQ and Ro 65-6570, were used as positive controls in the EPM. NOP(+/+) and NOP(-/-) mice were used to evaluate the selectivity of those compounds that induced anxiolytic-like behaviors. The forced swim test (FST) was used to evaluate the effects of compounds on depressive-like behaviors. Nortriptyline and the NOP antagonists, UFP-101 and SB-612111, were used as positive controls in the FST. The effects of N/OFQ, UFP-101, SB-612111, UFP-113, [F/G]N/OFQ(1-13)NH2, and AT-090 were assessed in the methylphenidate-induced hyperlocomotion (MIH) test; in this assay valproate was used as positive control. The G protein and β-arrestin 2 transduction pathways of NOP receptor agonists (N/OFQ and Ro 65-6570), antagonist (UFP-101), and partial agonists (UFP-113, [F/G]N/OFQ(1-13)NH2, and AT-090) were also evaluated using an innovative assay that measures a bioluminescence resonance energy transfer process. For this, cell lines permanently co-expressing the NOP receptor coupled to luciferase (energy donor), and green fluorescent protein (energy acceptor) coupled to one of the effector proteins (G protein or β-arrestin 2) were used. Results: Diazepam (1 mg/kg), N/OFQ (1 nmol), Ro 65-6570 (0.1 mg/kg), and AT-090 (0.01 mg/kg) induced anxiolytic-like effect in mice in the EPM. The effects of Ro 65-6570 and AT-090 were selective to NOP receptor. UFP-113 (0.01-1 nmol) and [F/G]N/OFQ(1-13)NH2 (0.1-3 nmol) were inactive in the EPM. In the FST, nortriptyline (30 mg/kg), UFP-101 (10 nmol), SB-612111 (10 mg/kg), UFP-113 (0.01 and 0.1 nmol), and [F/G]N/OFQ(1-13)NH2 (0.3 and 1 nmol) induced antidepressant-like effects, while AT-090 (0.001-0.1 mg/kg) was inactive in this assay. The effects of UFP-113 and [F/G]N/OFQ(1-13)NH2 were selective to NOP receptor. Valproate (400 mg/kg) counteracted methylphenidate (MPH, 10 mg/kg)-induced hyperlocomotion in mice in the open field. N/OFQ (1 nmol), UFP-113 (0.01-0.1 nmol), and [F/G]N/OFQ(1-13)NH2 (1 nmol) were also able to reduce the MPH-induced hyperlocomotion, without changing the locomotor activity per se. The effect of UFP-113 was selective to NOP receptor. The UFP-101 (10 nmol), SB-612111 (10 mg/kg), and AT-090 (0.001-0.03 mg/kg) did not change the hyperlocomotor effect of methylphenidate. In vitro, N/OFQ and Ro 65-6570 behaved as NOP full agonists for G-protein and β-arrestin 2 pathways. AT-090 behaved as NOP receptor partial agonist for both transduction pathways, while UFP-113 and [F/G]N/OFQ(1-13)NH2 behaved as partial agonists and antagonists of NOP receptor for NOP/G protein and NOP/β-arrestin 2, respectively. UFP-101 behaved as NOP receptor antagonist for both transduction pathways. Conclusion: NOP ligands producing same effects on NOP/G protein interaction (partial agonism), but with opposite effects on β-arrestin 2 recruitment (partial agonism vs antagonism), can promote different in vivo effects on anxiety and mood as it was observed in the behavioral tests. This work corroborates the potential of NOP receptor as an innovative pharmacological target for the treatment of emotional disorders.

Efeitos in vivo e in vitro de agonistas parciais do receptor NOP: implicações para o tratamento da ansiedade, depressão e mania

Introduction: This study aimed to investigate the effects of the two peptide NOP partial agonists (UFP-113 and [F/G]N/OFQ(1-13)NH2) and the non peptide NOP partial agonist (AT-090) in the mouse emotional behavior as well as in the intracellular transduction pathways following the receptor binding. Methods: Male Swiss or CD-1 mice were used in this study together with NOP(+/+) and NOP(-/-) mice. The elevated plus maze (EPM) was used to evaluate the effects of compounds on anxiety-like behaviors. Diazepam and the NOP agonists, N/OFQ and Ro 65-6570, were used as positive controls in the EPM. NOP(+/+) and NOP(-/-) mice were used to evaluate the selectivity of those compounds that induced anxiolytic-like behaviors. The forced swim test (FST) was used to evaluate the effects of compounds on depressive-like behaviors. Nortriptyline and the NOP antagonists, UFP-101 and SB-612111, were used as positive controls in the FST. The effects of N/OFQ, UFP-101, SB-612111, UFP-113, [F/G]N/OFQ(1-13)NH2, and AT-090 were assessed in the methylphenidate-induced hyperlocomotion (MIH) test; in this assay valproate was used as positive control. The G protein and β-arrestin 2 transduction pathways of NOP receptor agonists (N/OFQ and Ro 65-6570), antagonist (UFP-101), and partial agonists (UFP-113, [F/G]N/OFQ(1-13)NH2, and AT-090) were also evaluated using an innovative assay that measures a bioluminescence resonance energy transfer process. For this, cell lines permanently co-expressing the NOP receptor coupled to luciferase (energy donor), and green fluorescent protein (energy acceptor) coupled to one of the effector proteins (G protein or β-arrestin 2) were used. Results: Diazepam (1 mg/kg), N/OFQ (1 nmol), Ro 65-6570 (0.1 mg/kg), and AT-090 (0.01 mg/kg) induced anxiolytic-like effect in mice in the EPM. The effects of Ro 65-6570 and AT-090 were selective to NOP receptor. UFP-113 (0.01-1 nmol) and [F/G]N/OFQ(1-13)NH2 (0.1-3 nmol) were inactive in the EPM. In the FST, nortriptyline (30 mg/kg), UFP-101 (10 nmol), SB-612111 (10 mg/kg), UFP-113 (0.01 and 0.1 nmol), and [F/G]N/OFQ(1-13)NH2 (0.3 and 1 nmol) induced antidepressant-like effects, while AT-090 (0.001-0.1 mg/kg) was inactive in this assay. The effects of UFP-113 and [F/G]N/OFQ(1-13)NH2 were selective to NOP receptor. Valproate (400 mg/kg) counteracted methylphenidate (MPH, 10 mg/kg)-induced hyperlocomotion in mice in the open field. N/OFQ (1 nmol), UFP-113 (0.01-0.1 nmol), and [F/G]N/OFQ(1-13)NH2 (1 nmol) were also able to reduce the MPH-induced hyperlocomotion, without changing the locomotor activity per se. The effect of UFP-113 was selective to NOP receptor. The UFP-101 (10 nmol), SB-612111 (10 mg/kg), and AT-090 (0.001-0.03 mg/kg) did not change the hyperlocomotor effect of methylphenidate. In vitro, N/OFQ and Ro 65-6570 behaved as NOP full agonists for G-protein and β-arrestin 2 pathways. AT-090 behaved as NOP receptor partial agonist for both transduction pathways, while UFP-113 and [F/G]N/OFQ(1-13)NH2 behaved as partial agonists and antagonists of NOP receptor for NOP/G protein and NOP/β-arrestin 2, respectively. UFP-101 behaved as NOP receptor antagonist for both transduction pathways. Conclusion: NOP ligands producing same effects on NOP/G protein interaction (partial agonism), but with opposite effects on β-arrestin 2 recruitment (partial agonism vs antagonism), can promote different in vivo effects on anxiety and mood as it was observed in the behavioral tests. This work corroborates the potential of NOP receptor as an innovative pharmacological target for the treatment of emotional disorders.

Inibição dos efeitos locais induzidos pelas peçonhas das serpentes Bothrops erythromelas e Bothrops jararaca pelo extrato aquoso das folhas de Jatropha mollissima (Pohl) Bail

Snakebites are a serious public health problem in tropical and subtropical countries and Bothrops genus is responsible for the accidents in Brazil and throughout Latin America (90% of cases). The local effects (pain, edema, hemorrhage and myonecrosis) and systemic (cardiovascular alterations, shock and blood clotting disorders) caused by the venom of Bothrops are due to the numerous protein and non-protein components, which are part of the constitution of the poison. The only form of therapy is scientifically validated antivenom serum therapy which, however, is not effective with respect to local effects produced, risk of immunological reactions, high cost and difficult access in some regions. Thus, the search for new alternatives to serum therapy becomes important, and in this context, many medicinal plants have been highlighted by the popular use as antiophidic. Among these plants, we can mention the species Jatropha mollissima (Euphorbiaceae) which has popular use in traditional medicine as antiophidic, anti-inflammatory, antimicrobial and antipyretic. Therefore, this study aims to evaluate the neutralizing potential of local effects induced by the venom of Bothrops erythromelas and Bothrops jararaca with the aqueous extract of the leaves of J. mollissima. The leaf extracts were prepared by decoction, fractionated (by liquid-liquid partition) and characterized by thin layer chromatography (TLC) and High Performance Liquid Chromatography (HPLC). Antiophidic activity of the extract was evaluated in model of paw edema, peritonitis, bleeding and myotoxicity induced by venoms of B. jararaca and B. erythromelas. In all models, the extract was evaluated by intraperitoneal route at the doses of 50, 100 and 200 mg/kg, administered 30 minutes prior to injection of the venom (pretreatment protocol). Stains suggestive of the presence of flavonoids: apigenin, luteolin, orientin, isoorientin, vitexin and vitexin-2-O-rhamnoside were detected in the extract by co-CCD. By means of HPLC were identified isoorientin, orientin, vitexin and isovitexin. All tested doses of J. mollissima extract reduced the paw edema induced by the venom with intensity similar to dexamethasone. The aqueous extract of J. mollissima leaves on all evaluated doses, inhibited cell migration induced by B. jararaca and B. erythromelas promoting inhibition of recruitment of mononuclear cells and the polymorphonuclear cells. Local bleeding induced by B. jararaca venom was significantly inhibited by the extract. Both venoms were inhibited by the extract in myotoxic activity. These results indicate that the aqueous extract of J. mollissima leaves have snakebite potential, particularly with respect to local effects, which may justify the use of this plant in traditional medicine and complementary therapy as anti-venom serum.

Inibição dos efeitos locais induzidos pelas peçonhas das serpentes Bothrops erythromelas e Bothrops jararaca pelo extrato aquoso das folhas de Jatropha mollissima (Pohl) Bail

Snakebites are a serious public health problem in tropical and subtropical countries and Bothrops genus is responsible for the accidents in Brazil and throughout Latin America (90% of cases). The local effects (pain, edema, hemorrhage and myonecrosis) and systemic (cardiovascular alterations, shock and blood clotting disorders) caused by the venom of Bothrops are due to the numerous protein and non-protein components, which are part of the constitution of the poison. The only form of therapy is scientifically validated antivenom serum therapy which, however, is not effective with respect to local effects produced, risk of immunological reactions, high cost and difficult access in some regions. Thus, the search for new alternatives to serum therapy becomes important, and in this context, many medicinal plants have been highlighted by the popular use as antiophidic. Among these plants, we can mention the species Jatropha mollissima (Euphorbiaceae) which has popular use in traditional medicine as antiophidic, anti-inflammatory, antimicrobial and antipyretic. Therefore, this study aims to evaluate the neutralizing potential of local effects induced by the venom of Bothrops erythromelas and Bothrops jararaca with the aqueous extract of the leaves of J. mollissima. The leaf extracts were prepared by decoction, fractionated (by liquid-liquid partition) and characterized by thin layer chromatography (TLC) and High Performance Liquid Chromatography (HPLC). Antiophidic activity of the extract was evaluated in model of paw edema, peritonitis, bleeding and myotoxicity induced by venoms of B. jararaca and B. erythromelas. In all models, the extract was evaluated by intraperitoneal route at the doses of 50, 100 and 200 mg/kg, administered 30 minutes prior to injection of the venom (pretreatment protocol). Stains suggestive of the presence of flavonoids: apigenin, luteolin, orientin, isoorientin, vitexin and vitexin-2-O-rhamnoside were detected in the extract by co-CCD. By means of HPLC were identified isoorientin, orientin, vitexin and isovitexin. All tested doses of J. mollissima extract reduced the paw edema induced by the venom with intensity similar to dexamethasone. The aqueous extract of J. mollissima leaves on all evaluated doses, inhibited cell migration induced by B. jararaca and B. erythromelas promoting inhibition of recruitment of mononuclear cells and the polymorphonuclear cells. Local bleeding induced by B. jararaca venom was significantly inhibited by the extract. Both venoms were inhibited by the extract in myotoxic activity. These results indicate that the aqueous extract of J. mollissima leaves have snakebite potential, particularly with respect to local effects, which may justify the use of this plant in traditional medicine and complementary therapy as anti-venom serum.