995 resultados para ddc: 510


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报道GaAs/GaAlAs中红外(3~5μm)量子阱探测器和双色量子阱红外探测器的制备和性能。GaAs/GaAlAs中红外量子阱探测器是光伏型,探测峰值波长为5.3μm,85K下的500K黑体探测率为3.0×10~9cm·Hz~(1/2)/W,峰值探测率达到5×10~(11)cm·Hz~(1/2)/W,阻抗为50MΩ。GaAs/GaAlAs双色量子阱红外探测器是偏压控制型的两端器件,在零偏压下该探测器仅在3~5μm波段有响应,响应峰值波长为5.3μm,85K温度下550K黑体探测率为3.0×10~9cm·Hz~(1/2)/W,当偏压为2V时,该探测器的响应切换到8~12μm波段,峰值响应波长为9.0μm,85K温度下的黑体探测率为1.0×10~9cm·Hz~(1/2)/W。

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国家自然科学基金

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林窗是森林中经常发生的重要中小尺度干扰,在森林天然更新中起着重要作用。灌木和草本植物通过与乔木幼苗竞争林窗资源(尤其是光)而影响林窗树种更新,因此,研究林窗光环境时空特征及其与下层植被关系对揭示林窗树种更新机制具有重要意义。然而,林窗光环境的时空异质性及有效测量方法的缺乏导致这方面研究很少。本研究提出了林窗立体结构的相片测量法;基于林窗立体结构、坡度和坡向改进了目前广泛用于估测林窗光环境的林窗光指数(gap light index, GLI)模型,并提出了林窗内任意位置最大光照时长(potential sunshine duration, PSD)的计算方法;采用改进的GLI和PSD分析了东北次生林3个不同大小林窗(110,270,510 m2)及其对应的9个模拟林窗中2007年光环境的时空特征;通过在12个人工林窗中连续3年的植被调查,研究了林窗形成后前3年下层木本和草本植物对4个光强梯度的响应,主要结论如下: 1. 林窗立体结构和光环境的测量方法:1) 等角椭圆扇形法计算的林窗面积比目前使用较多的等角多边形法具有更高的精度;2) 半球面影像法可以测量林窗面积和形状,且精度较高;3) 双半球面影像法不仅可以测量林窗面积、形状,还可以测量任意方位林窗边缘木高度;4) 改进的GLI可以快速、精确计算林窗任意位置的直射光和散射光;5) PSD可用于分析林窗光照强度时空特征。 2. 东北次生林林窗光环境时空特征:1) 水平空间结构(地面层):光环境空间异质性高,最大值位于林窗北部,坡向对光分布格局影响大而对光强影响小,光强随林窗面积的增大和边缘木高度的降低而增加;2) 垂直空间结构(林窗南北轴):不同高度层光强最大值均位于林窗南北轴中心偏北,南北轴距地面越高光强最大值则越靠近轴中心;光强随距地面高度下降而衰减,最大衰退速率点位于轴中心偏南,靠近该点光强衰退线呈指数形,远离该点则趋于直线形;3) 光成分特征(地面层南北轴):南坡林窗整个南北轴上直射光大于散射光,北坡模拟林窗南半轴散射光大于直射光,而北半轴相反;南坡林窗中直射光的均值及其变化范围都比其对应的北坡模拟林窗大,然而,坡向对散射光影响不大;南坡林窗和北坡模拟林窗北半轴的全光和直射光均比南半轴大,但散射光没有这种显著的规律;4) 时间特征:不同月份PSD在相同大小林窗中分布格局十分相似,6月份林窗中被光照射面积和PSD均值最大,而10月份最小;各月PSD在南北轴上从南到北逐渐增加,在东西轴上自西向东缓慢增加。 3. 林窗早期下层植被对光强梯度的响应:1) 木本植物物种丰富度随光照强度的增加而增高,且在林窗形成后的第2和3年均存在显著相关性;2) DCA排序第一轴表明,木本植物物种组成仅第1年在4个光强梯度间存在显著差异,而在各年间存在显著差异;草本植物物种组成在4个光强梯度上存在显著差异,但在各年间不存在显著差异;3) 木本植物生物体积(biovolume)随光强梯度升高而增加,而植株密度不存在显著变化;生物体积逐年增加,平均年增长率为20.3%,植株密度逐年下降,平均年减少率为30.0%;4) 草本层盖度和高度在4个光强梯度间均不存在显著差异,但在林窗形成后的第2、3年均显著比第1年大。

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为了探讨远紫外辐射对植物的损伤机理和紫杉的濒杉机制,本文就远紫外辐射对紫杉幼苗针叶膜脂过氧化及内源保护物质的影响进行了模拟研究,初步得到如下结果:UV-C 辐射紫杉针叶离体叶绿体可使膜脂过氧化产物丙二醛(MDA)含量增加,类胡萝卜素含量和光系统II(PS II)电子传递少性下降。UV-BC 辐射紫杉幼苗针叶可使叶绿体超氧离子自由基(O~-_2),单线态氧(~O_2),针叶有机自由基产额和H_2O_2含量有不同程度增加。针叶MDA,组织自动氧化速率及质膜相对透性也随辐射时间进程面增加。UV-BC处理初期超氧化物歧化酶(SOD),过氧化氢酶(CAT)活性及谷胱甘肽(GSH)含量均被诱导增高,21d后SOD活性开始下降,而GSH 含量和CAT活性始终高于对照。维生素C(ASA),类胡萝卜素(Car),叶绿素(chl),PS II 电子传递活性在处理期间始终呈下降趋势,其中ASA下降最明显。可溶性蛋白21d前变化不大,之后开始下降。外源活性氧清除剂苯甲酸钠和抗坏血酸对针叶膜脂过氧化有抑制作用;甲基紫精和DDC 对针叶膜脂过氧化有促进效果。根据上述结果推测,紫杉的UV-BC伤害可能是由于活性氧产生过剩和清除系统水平下降,而引起的膜脂过氧化损伤。

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横断山地区是一个十分自然的植物区系地区,在中国植物区系分区中是作为泛北极植物区中国-喜马拉雅亚区中的一个地区,其种子植物区系具有丰富的科、属、种,地理成分复杂,特有现象和替代现象明显。该地区作为植物区系和生物多样性的研究热点地区,长期以来极受中外植物学家关注。横断山脉东缘是中国-喜马拉雅和中国-日本植物区系的交汇过渡区域,北部的岷江流域以及南部的金沙江流域,孕育了该区丰富的物种资源和植被资源。而岷江干热河谷和金沙江干热河谷的相似性和相关性,更为该区的植物区系和生物多样性南北的对比研究提供了有利的条件。 本研究选择的九顶山西坡和龙肘山分别位于横断山区北部和南部,九顶山属岷江流域而龙肘山属金沙江流域。本研究结合植物区系研究和生物多样性研究,对该区的植物资源进行调查。通过样带调查和样线踏查结合,大量详实的野外样方调查和标本采集,进行传统的区系研究和生物多样性研究。研究该区物种多样性的海拔梯度格局及其潜在的影响影子,并利用新的区系评估质量方法对九顶山西坡的植物区系质量进行定量的研究,以期能更为深刻的理解该区的植物资源,为该区的资源保护和利用提供合理可行的建议。主要研究结论如下: 1)九顶山西坡植物区系的性质和特点 经鉴定和统计,九顶山西坡共有1707 种维管植物,分属617 属和140 科,其中种子植物1616 种,分属572 属117 科。就科的分布区成分构成而言,该区系的热带成分与温带成分相当,热带成分略占优势,表明九顶山西坡的植物区系与热带植物区系有较强的联系。但是,在九顶山西坡属的分布区类型所占的比例上,温带成分远远超过了热带成分,本区的种子植物分布表现出明显的温带性质。且温带分布类型的许多物种组成了九顶山西坡植被的建群种和优势种,是本区系最重要的成分,充分体现了本区系的温带性质。 2)九顶山西坡不同植被带的生物多样性海拔梯度格局 基于对土门-断头崖、茶山-九顶山、雁门沟-光光山三条垂直植被样带的调查,我们发现九顶山西坡的生物多样性沿海拔梯度的变化呈现出一定的规律性,不同样带之间有一定差异。就三条样带的物种组成相似性来看,虽然土门-断头崖样带属于涪江水系,而茶山-九顶山样带和雁门沟-光光山样带属于岷江水系,但不同水系对该区物种组成的影响并不明显。三条样带中,草本层物种丰富度均远远大于灌木层和乔木层,而以乔木层物种丰富度最低;α-多样性指数随着海拔梯度的变化在土门-断头崖样带中呈现单一下降趋势,在茶山-九顶山样带表现为双峰模型,而在雁门沟-光光山样带则表现为不显著波动变化;均匀度指数在土门-断头崖样带呈现出单一下降的趋势,在雁门沟-光光山样带表现为凹形曲线,而在茶山-九顶山样带却无明显的变化规律。β-多样性指数在土门-断头崖样带和茶山-九顶山样带呈现出明显的波动状态,植被类型替代现象明显;而在雁门沟-光光山样带却并未有十分显著的转折点,因其水平植被带受到干扰,同海拔替代现象不显著。 3)九顶山西坡维管植物丰富度的海拔梯度格局 我们考察了九顶山西坡和两条垂直样带(土门-断头崖和雁门沟-光光山样带)的不同分类等级(包括科、属、种)和不同生活型物种(乔木、灌木、禾草、蕨类和其它草本)的丰富度沿着海拔梯度的分布。结果发现,物种的丰富度海拔梯度格局具有不同的模式,单一下降和中间膨胀格局依然是其主流。不同生活型的物种具有不同的丰富度格局,但是对于环境需求相似的类型具有较相似的丰富度格局。不同的丰富度格局可能由多因素导致,包括:气候,海拔跨度,面积,人为干扰等等。 4)九顶山西坡区系质量评估 我们尝试使用传统的区系质量评估方法对九顶山西坡的区系质量进行评估,并尝试使用一种新的区系质量评估体系对该区的区系进行评价。在九顶山西坡随着海拔梯度的上升,平均保守性系数在各条植被带中均呈现出逐渐上升的趋势。区系质量指数随着海拔的升高都表现为双峰模型,在植被交错区区系质量指数相对较高,而在海拔的两极,区系质量指数都很低。大部分地区使用新方法计算所得的加权平均保守性系数和区系质量指数都比传统方法计算的平均保收性系数和区系质量指数要高,说明在九顶山西坡的三条样带中,大部分地区都是那些保守性系数较高的物种占据优势,同时也表明九顶山西坡具有很高质量的区系和自然植被。 5)龙肘山种子植物区系的性质和特点 龙肘山种子植物区系的物种较为丰富,共有154 科,544 属,1156 种。科的优势十分明显,单种属和寡种属数量众多,说明本区系植物成分较为复杂、起源古老、物种多样性指数较高。地理成分复杂,分布类型多样,其中热带成分在总数量上高于温带成分,但是许多温带成分的属是该区植被的重要建群类群和优势类群,表现出明显的亚热带性质。 6)龙肘山生物多样性的现状和特点 在海拔梯度上,龙肘山地区无论是科、属、种的数量,还是不同等级分类单元之间的数量比,均呈现先升后降的趋势,并在中海拔地区达到峰值。物种多样性指数从总体上来说变化幅度不大,略有先升后降的趋势,在中海拔梯度物种多样性最高。乔、灌、草三层的多样性指数表现出乔木层<灌木层<草本层的特征;乔木层均匀度的变化很大,而灌木层和草本层均匀度的变化较小;灌木层均匀度的波动又强于草本层。β-多样性指数呈现单峰模式,中海拔地区最高。就龙肘山东、西坡物种多样性相比较而言,两者虽然在数值上交替上升,但是却体现出了较为一致的趋势,但西坡因受到干热河谷气候的影响,其平均气温要高于东坡,导致了东坡植物群落和物种的分布比西坡要低。在区系成分构成上,低山区的相同海拔段,西坡的热带亚热带成分所占的比例要比东坡高,这是因为西坡的平均气温比东坡稍高,导致了热带、亚热带物种分布更多。而随着海拔的上升,东、西两坡的气候、土壤等条件趋于一致,其植物区系成分的构成格局也趋于一致。 The Hengduan Mountain region is a very natural floristic region; it belongs toChina-Himalaya sub-region of Holarctic region in floristic subarea of China. The flora in this areais rich in family, genus and species; has a very complex composition of geographical elements;especially with high richness of endemic species and obvious substitution phenomenon. Thisregion as a hot-spot area of floristic and biodiversity, has fascinated biologists in the world for along time. The eastern range of Hengduan Mountain is the transition zone of China – Himalayaforest sub-region and China-Japan forest sub-region in floristic. The water systems are quitedifferent, Minjiang River in the north and Jishajiang River in the south grow quit different but alsoabundant plant species and vegetation resources. The similarity and correlativity of Minjiang River dry valleys and Jinshajiang River dry valleys have provided advantageous condition tocontrast flora and biodiversity between north and south. In the present study, the Jiuding Mountainlies in the north of Hengduan Mountain and belongs to Minjiang River, and the LongzhouMountain lies in the south of Hengduan Mountain and belongs to Jinshajiang River. In our study, we combined the methods of floristic research and biodiversity investigation toexplore the resources of plant species and vegetations; sampled with transects along the altitudinalgradients and also with transverse straps with similar elevation; collected the vascular plant specimen with sampling plots of ecology. We explored the plant species richness patterns alongaltitudinal gradients and discussed the underlying factors aroused these patterns; and used a novelmethod to assess the quality of Jiuding Mountain’s flora. All for a deeper comprehension of the plant recourses of this region; and provided feasible and reasonable suggestion for the protectionof resources. The results were as follows: 1 The characteristic of the flora of the west slope of Jiuding Mountain We had collected 1707 species of vascular plants belonging to 617 genera in 140 families inthe west slope of Jiuding Mountain,in which included 1616 seed plant species belonging to 572genera and 117 families. As for the composition of the areal types of the Families of seed plants,tropic components and temperate components are well-balanced, and percentage of tropicscomponents is higher than that of temperate ones for a litter bit. This shows the flora in the westslope of Jiuding Mountain has strong relationship with the tropic flora. But for the composition ofthe areal types of genera, temperate components have far exceeded the tropics ones, indicated thewhole flora with a conspicuous temperate character. Temperate components possess maximumproportion in the west slope of Jiuding Mountain, and many of them belong to constructivespecies and dominant species in the vegetation, are most important components in JiudingMountain’s Flora, also have embodied the temperate character of this area sufficiently. 2 Biodiversity patterns along altitudinal gradients in different vegetation transects in the westslope of Jiuding Mountain Based on the investigation of three vegetation transects (including Tumen-Duantouya transect,Chashan-Jiudingshan transect and Yanmengou-Guangguangshan Transect) in the west slope ofJiuding Mountain, we found the change of biodiversity along the altitude gradients displayedcertain regularity, but have differences among different transects. The three transects belong todifferent water systems; the Tumen-Duantouya transect belongs to Fujiang River, and the othertwo belong to Minjiang River. From the similarity of species compositions of different transects,we found different water system didn’t show obvious impact on the species composition. In all thethree transects, the species richness of herb layer was remarkably higher than shrub and tree layer,and the species richness of tree layer was the lowest one. With the increasing of the altitude, theline of α-diversity was monotonically decreasing curve in Tumen-Duantouya transect, andbimodal curve in Chashan-Jiudingshan transect, but in Yanmengou-Guangguangshan transectshowed a wave-like curve although not very obvious. Species evenness showed monotonicallydecreasing trends in Tumen-Duantouya transect, and very low at mid-altitude in Yanmengou-Guangguangshan transect, but in Chashan-Jiudingshan transect changed irregularly. Changes inβ-diversity corresponded with the transition of vegetation in the Tumen-Duantouya transect andChashan-Jiudingshan transect, and the curve of β-diversity along altitude had obvious turningpoint; but in Yanmengou-Guangguangshan transect had no obvious turning point, and thesubstitution phenomenon was not obvious, transverse vegetation straps distributed interlaced. 3 Richness patterns of vascular plant species along altitude in the west slope of Jiuding Mountain Direct gradient analysis and regression methods were used to describe the species richnesspatterns along the altitudinal for Mt. Jiuding, as well as separately for Tumen-Duantouya Transectand Yanmengou-Guangguangshan Transect. Altitudinal gradient of diversity of units at differenttaxonomic level (including Family, Genus and Species) and at different life form (including tree,shrub, pteridophyte, grass and other herb) were tested to find differences among the richnesspattern. We found altitudinal richness also shows different patterns, and both monotonicallydecreasing pattern and hump-shaped pattern can be founded in vascular species richness. Speciesin different life forms show different altitudinal patterns, but those species with similarrequirements to environmental conditions show similar richness patterns along altitudinalgradients. Different richness patterns can be aroused by different climate, different altitudinal span,area factor, anthropogenic factor and so on. 4 Floristic quality assessments in the west slope of Jiuding Mountain We used both the conventional method broadly adopted in the USA and the new one toassess the floristic quality in the west slope of Jiuding Mountain. The Mean Coefficient ofConservatism (MC) had the trend of increment along the altitudinal gradients. The FloristicQuality Index (FQI) was a bimodal curve with increasing of elevation; FQI got maximum valuesin the transition zones of different vegetations in the middle altitude, and had very low values atthe two end of elevation. In most areas of the west slope of the Jiuding Mountain, the resultscalculated using the new methods were higher than those using the conventional method. Thisindicated the dominant species of the communities had very high coefficients of conservatism inmost areas of Jiuding Mountain, and the communities are relatively kept pristine and the habitats very integrative. 5 The characteristic of the flora of Longzhou Mountain The flora of Longzhou Mountain has very abundant in species composition; there are about1156 species of seed plants belonging to 544 genera in 154 families. In which, twelve families with more than 20 species include totally 232 genera and 532 species, and form the majority of itsflora. The origin of its flora is old, monospecific genera and oligotypic genera amounts to 510 innumber, which constitute 93.75% of total number of genera. The geographical components arevarious in Longzhou Mountain, the majority of flora are temperate and pantropic ones. The tropiccomponents overtopped temperate components on genera quantity, but many temperatecomponents belong to constructive species and dominant species in the vegetation, and the wholeflora shows an obvious subtropical character. 6 Current situation and characteristic of biodiversity in Longzhou Mountain With the increasing of altitude, the number of species, genus, family and the ratios ofdifferent taxonomic levels all displayed a trend of descending after rising first, and peaked atmiddle height area. The change of α-diversity was not very acutely, with the trend of descendingafter rising first in some degree, the middle height area had highest α-diversity. As studying thetree layer, shrub layer and herb layer respectively, the Shannon-Wiener index was in followingorder: tree layer < shrub layer < herb layer; the change of evenness was more complicatedly thanthat of diversity, the tree layer changed acutely, but the shrub layer and herb layer fluctuatedsmoothly. Changes in β-diversity also showed the trend of descending after rising first. TheJaccard index and Cody index all peaked at the middle height forest area. As for the comparison ofplant diversity and evenness between the west and east slope, the numerical values ascendedalternatively, but the trend of changing was similar. The distribution of similar plant communitiesand species in east slope were lower than the west slope for the influence of Jinsha River DryValley. As for the composition of different floristic components, in lower altitude area of westslope, the tropic and sub-tropic plants had higher ratio than east slope’s and even could be equal tothe temperate plants. With the increasing of elevation, the floristic composition become morelikely between the east and west slope and temperate plants dominated the flora.

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通过单因子和多因子摇瓶正交试验,确定了米曲霉液态发酵产氨基酰化酶的最佳发酵条件。优化发酵培养基组成(ρ/g L-1): 葡萄糖40,蔗糖10,可溶性淀粉20,蛋白胨2.5,马铃薯液1 000mL, pH自然。培养基装量50mL/250mL三角瓶,接种量4%。培养温度30℃,转速100 rmin-1,发酵时间42h。每50mL培养物的总酶活由优化前的2627U提高到7338U,是优化前的2.79倍。 研究了米曲霉氨基酰化酶的部分酶学性质,该酶催化反应的最适pH为7.0,最适温度为40℃,低浓度的Co2+(5×10-4mol/L)对酶活激活作用显著,催化反应过程中,底物浓度大于0.2 mol/L时,存在高浓度底物抑制酶活力现象。 初步探索了包埋法固定化米曲霉氨基酰化酶的载体,在实验的五种载体中,以海藻酸钠为载体包埋固定化米曲霉氨基酰化酶酶活保留率高,且操作简单,成本低廉。对包埋法固定化米曲霉氨基酰化酶酶学性质进行了研究,较游离米曲霉氨基酰化酶,最适温度未发生改变,最适pH向碱性范围偏移至8.0,对酸碱和热的稳定性增强,最适底物浓度增大到0.4 mol/L。 根据氨基酰化酶能立体专一水解L-氨基酰化物的特点,利用米曲霉氨基酰化酶对消旋苯丙氨酸进行了拆分。在米曲霉氨基酰化酶选择性的作用于底物N-乙酰-L-苯丙氨酸,得到L-苯丙氨酸后,通过732阳离子树脂和结晶法分别将L-苯丙氨酸和N-乙酰-D-苯丙氨酸分离,N-乙酰-D-苯丙氨酸通过酸水解脱去乙酰基得到D-苯丙氨酸,拆分得到光学纯度为98%的L-苯丙氨酸(收率84.8%)和光学纯度为92.3%的D-苯丙氨酸(收率89.5%)。 separate factors tests and orthogonal experiments,the optimum fermentation conditions of aminoacylase –producing Aspergillus oryzae were determined, as follows(ρ/g L-1),glucose 40,sucrose 10,soluble starch 20,peptone 2.5,potato juice 1000ml, inoculation volume 4%and fermentation temperature 30℃,rotation speed 100rmin-1.The highest total enzyme activity ,7338μ,was obtained after fermentation for 42 h, increased by 279% compared with the original value of 2627μbefore optimization. We dicussed partial characteristics of aminoacylase. The optimal pH and temperature of aminoacylase were 7.0 and 40℃ respectively. Low- concentration Co2+ (5×10-4mol/L)activated the aminoacylase remarkably while high-concentration substrate lowered the aminoacylase . Five vectors has been used for immobolizing the enzyme and calcium alginate showed to be the best one for it had the slightest influence on the enzyme activity, easy to operate ,and low in price, comparing with other fours. The enzymatic charateristic study showed that its optimum temperature didn’t change, but the optimum pH and substrat concentration were higher after immobilization. The stability of immobolized enzyme to acid, alkaline and heat rised as well. The aminoacylse from Aspergillus oryzae was used to resolute racemic phenylalanine to obtain D-phenylalanine. After catalyzing process, we took two methods to separate D-phenylalanine .In end,L-phenylalanine was obtained with 98% optical purity in 84.8% yield, D-phenylalanine was obtained with 92.3% optical purity in 89.5% yield.

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本工作研究460 keV、3 MeV和308 MeV Xe23+辐照Al2O3单晶样品的光致发光特性。从经过460 keV Xe23+辐照后样品的光致发光测试结果可看到,波长为380、413和450 nm的发光峰明显增强,在390和564 nm处出现了新的发光峰。从3 MeV的Xe23+辐照后样品谱的变化可看到,在较低剂量条件下,516 nm(2.4 eV)和564 nm(2.2 eV)处的发光峰随辐照剂量增加而增强,且当剂量增到1×1016cm-2时,564 nm处的发光峰消失,只有516 nm(2.4 eV)处的发光峰较强。从308 MeV Xe23+辐照后样品的光致发光谱中可看到,357 nm(3.47 eV)和516 nm(2.4 eV)处的发光峰随着剂量增加明显增强。辐照后样品的FTIR谱显示:波数在460~510 cm-1和630 cm-1附近的吸收是Al2O3振动模式,经离子辐照后,吸收带展宽;1 000~1 300 cm-1间为Al—O—Al桥氧键的伸缩振动模式,高能辐照后的吸收带向低波数方向移动。

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本文介绍了近代物理研究所自行生长的CsI(Tl)晶体用于γ射线及重离子能量测量的结果。测量662keVγ射线时得到好于10%的能量分辨(采用光电倍增管读出),同时光输出可达到同类型晶体的130%;测量能量为30MeV/u左右的重离子时得到好于2%的能量分辨(采用光二极管读出)。

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研究了L-α-丙氨酸剂量计测量12C离子辐射的剂量学特性,实验证明丙氨酸剂量计适用于12C离子辐射的剂量学测量。另外,还研究了12C离子照射人外周血诱发的染色体畸变(双着丝粒+着丝粒环)的剂量效应,在0—8.0 Gy范围内拟合的最佳回归方程为Y=0.858 503D+0.361 5×10-2D2。