Efeito da adição de oxigênio na produção de glicerol quinase pela levedura recombinante de Pichia pastoris

O glicerol é um importante subproduto de processos fermentativos. Em algumas bebidas alcoólicas, como vinhos e vodcas contribuem com seu sabor tornando-a aceitável para o consumidor. Com o incentivo na produção de biocombustíveis, a disponibilidade do glicerol deve aumentar, já que é o principal subproduto gerado na produção do Biodiesel. A sua presença em alguns alimentos, pode identificar presença de microrganismos, já que pode ser uma fonte de carbono para eles. Devido às inúmeras utilizações do glicerol para diversos tipos de indústrias, a dosagem do glicerol se torna cada vez mais importante e rotineira. Isso incentiva novas pesquisas de métodos de doseamento. A enzima GK é presente em vários microrganismos e converte o glicerol em glicerol-3-fosfato. Com a utilização da enzima Glicerol -3- fofato desidrogenase, o glicerol - 3 – fosfato é convertido a di-hidroxi-acetona fosfato com a produção de NADH. Este pode ser quantificado através do espectrofotômetro, tornando-se um rápido e eficiente método para doseamento. A levedura recombinante Pichia pastoris foi escolhida pois foi inserida no seu DNA, o Gene GUT1 que expressa a GK em Saccharomyces cerevisiae. Além disso, a expressão enzimática é extracelular, diminuindo etapas no processo de isolamento da enzima GK. O trabalho consistiu em otimizar a produção de GK expressa pela levedura recombinante Pichia pastoris por meio da adição de oxigênio no biorreator de fermentação em escala de 2,5 L. Os resultados do presente estudo indicam o ponto ótimo de oxigenação em fermentador nos parâmetros estabelecidos é de 2,0 lpm tanto para a produção de enzimática quanto para biomassa formada

Efeitos da ingestão aguda do suco de laranja na resposta glicêmica e insulínica, níveis séricos de leptina e adiponectina, consumo energético e atividade antioxidante

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência do porta-enxerto, da configuração de extração e do período de maturação no teor de limonina e na aceitação sensorial do suco de laranja Pêra-Rio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência do boro no crescimento e acúnulo de nutrientes em porta-enxertos de caramboleira

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Queijo Prato com teor reduzido de gordura adicionado de enzima proteolítica: características físicas e sensoriais

The Prato cheese is the second most consumed cheese in Brazil. It is produced by milk enzymatic coagulation, and maturated for at least 25 days; it is classified as fatty cheese and of medium moisture. Due to the concern to health, the cheeses consumers have been seeking for products with low fat contents; however fat is essential for providing desirable sensory and physiologic characteristics, such as flavor, softness and texture to cheeses. Alterations on the technological processing of low fat cheeses have been made seeking for improved products, and the use of proteolytic enzymes has been a significant strategy. The meltability, color and sensory characteristics are fundamental quality indicators of the final products. This study reports the findings from the analyses on the physical and sensory characteristics of low fat Prato cheese with addition of proteolytic enzyme – fastuosain, that is extracted from unripe gravata fruit. The addition of fastuosain improved the quality of the product, as this additive neither affected the meltability, nor produced bitterness, which is a common unpleasant taste in low fat cheeses.

Histórico e aspectos tecnológicos do processamento da linguiça cuiabana

This paper reports the data on the cuiabana sausage historical aspect, and from the study performed on its technical/sensory characteristics. Four formulations of cuiabana-type sausage were prepared, varying the basic raw material (bovine, chicken and swine meats). For processing these sausages, the raw meats (beef, chicken and pork) were cut into cubes and together with the other ingredients (garlic, cheese, pepper and chives) they were weighed and homogenized. The resulting mixture was refrigerated for 24 hours for intensifying the flavor. Thereafter, it was stuffed into natural casings. These prepared samples were characterized on the physical-chemical profile and on the shelf life under refrigeration for over a seven day-storage in order to assess the microbiological contamination (total coliforms and fecal thermotolerant bacteria), sulphite-reducing clostridium, Salmonella spp., coagulase-positive Staphylococcus), and pH. The sensory evaluation and statistics analysis were carried out on the different types of laboratory- processed cuiabana sausage. Introducing over time changes in the cuiabana sausages formulation are positive factors; therefore, variations in the formulations it is feasible to get a better final product than that original one, and it might be resulted from the market demand.

Efeito da utilização de substitutos de gordura em queijos light

Cheeses are known to be sources of calcium, phosphorus and protein, important nutrients for a suitable nutrition. However, certain cheeses imply the ingestion of large amounts of fats, which can cause the development of coronary heart and carcinogenic diseases. Although consumers are aware of the necessity of reducing the fats intake, they are still not pleased with the quality of light cheeses available on the market, because the partial or total fat removal provides some undesirable changes, especially regarding to the product texture and flavor. In order to offer products nutritionally adequate and palatable, alternatives have been developed to improve the characteristics of light cheeses. Such alternatives include the use of fat substitutes, those additives that improve the functional and sensory characteristics of cheeses with reduced fat. Fat substitutes composed of proteins, carbohydrates and lipids, or a combination of them, help the retention of moisture and eliminate the undesirable characteristics of fat reduced-cheeses. In this context, this review aims at reporting the innovations and trends on the use of fat substitutes to produce light cheeses.

Avaliação da qualidade de óleos de fritura utilizados em restaurante universitário

Frying is a widely used method of preparing food, because it is a very fast and convenient process, and because it provides specific characteristics of color, flavor, odor, and texture, besides having great consumer acceptance. Therefore, the interest in the physiological effects that oils heated at high temperatures can cause to the human body arises. The aim of this work was to analyze the levels of frying used oil alteration in different kinds of foods served at the university restaurant at “Instituto de Biociências, Letras e Ciências Exatas” (IBILCE), in São José do Rio Preto-SP. In addition, it aims to make the restaurant aware of the good ways to fry and the commitment of providing good quality food to students and professionals of the Institute. With this purpose, determination of total polar compounds, conjugated dienoic acids, peroxide value, and fatty acid profile were analyzed. Two rapid tests were also used: 3M Fat Monitor and Oil Test kit. The results were compared with limits recommended for the disposal of oils and fats used in frying by the Brazilian Health Surveillance Agency (ANVISA), by other countries, and by other researches. Thus, it was concluded that the university restaurant provides the consumers with fried food of good quality, since none of the analyses showed results above recommended, although there is a need of improvement in the quality control of the oil used, in order to avoid unnecessary costs to the university restaurant.

Efeitos dos derivados vegetais de condimentos nas características de qualidade e vida de prateleira de linguiça de frango fresca

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Utilização de informações genômicas para o melhoramento genético de características da carne em bovinos da raça Nelore

Pós-graduação em Genética e Melhoramento Animal - FCAV

Atividade antioxidante do suco de laranja processado por alta pressão hidrostática

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Nitrato, nitrito e sorbato em produtos cárneos consumidos no Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da citotoxicidade e atividade antioxidante de plantas condimentares

Pós-graduação em Biociências - FCLAS

Do politicamente correto ao incorretamente polido

The text argues that a linguistic analysis paired with the appreciation of the context of current social stands is highly legitimate, observing, however, that, in different situations, this so-called investigative-appreciative attitude has been transformed in actions and reactions that might become vulgarized in common place, or might even become disfigured in stupidity. The distortions in the assessment of the discourse of the other are now a widespread, the hunt for what is now conventionalized as the politically incorrect in language is just a sample of such a distortion. In this presentation, a number of cases of such a nature is analyzed against cases in which, inversely, the use of language is markedly polite, but the deviation of conduct is made more evident. The objective is to evaluate both cases via the role of intersubjectivity in the construal of discourse, aiming at verifying its conceptual counterpart, with special attention to evidence on the mutual conceptual engagement between interlocutors.

Tipos de condução de hastes na produção e na qualidade de mini tomate em manejo orgânico

Pós-graduação em Agronomia (Horticultura) - FCA