Effects of Cobalt Chloride in Junctional Epithelium and Reduced Enamel Epithelium of the Rat Maxillary First Molar

Cobalt is one of the main components of cast metal alloys broadly used in dentistry. It is the constituent of 45 to 70% of numerous prosthetic works. There are evidences that metal elements cause systemic and local toxicity. The purpose of the present study was to evaluate the effects of cobalt on the junctional epithelium and reduced enamel epithelium of the first superior molar in rats, during lactation. To do this, 1-day old rats were used, whose mothers received 300mg of cobalt chloride per liter of distilled water in the drinker, during lactation. After 21 days, the rat pups were killed with an anesthetic overdose. The heads were separated, fixed in ""alfac"", decalcified and embedded in paraffin. Frontal sections stained with hematoxylin and eosin were employed. Karyometric methods allowed to estimate the following parameters: biggest, smallest and mean diameters, D/d ratio, perimeter, area, volume, volume/area ratio, eccentricity, form coefficient and contour index. Stereologic methods allow to evaluate: cytoplasm/nucleus ratio, cell and cytoplasm volume, cell number density, external surface/basal membrane ratio, thickness of the epithelial layers and surface density. All the collected data were subjected to statistic analysis by the non-parametric Wilcoxon-Mann-Whitney test. The nuclei of the studied tissues showed smaller values after karyometry for: diameters; perimeter, area, volume and volume/area ratio. Stereologically, it was observed, in the junctional epithelium and in the reduced enamel epithelium, smaller cells with scarce cytoplasm, reflected in the greater number of cells per mm3 of tissue. In this study, cobalt caused epithelial atrophy, indicating a direct action on the junctional and enamel epithelium.

Effects of Cadmium on the Rat Salivary Glands, During Lactation

Cadmium (Cd) in air, drinking water and food has the potential to affect the health of people, mainly those who live in highly industrialized regions. Cd affects placental function, can cross the placental barrier and directly modify fetal development. Once the organism is particularly susceptible to the exposition to the Cd during the perinatal period, and that this metal can be excreted in the milk, the aim of the present work was to study the effects of the constant exposition to drinkable water containing low levels of Cd during the lactation, on the salivary glands of the rat. Female rats received ad libitum drinking water containing 300mg/l of CdCl2 throughout the whole lactation. Control animals received a similar volume of water without Cd. Lactant rats (21 day old) were killed by lethal dose of anesthetic. The salivary glands were separated, fixed in ""alfac"" solution for 24 h, and serially sectioned. The 6 mu m thick sections were stained with hematoxylin and eosin. Nuclear glandular parameters were estimated, as well as cytoplasm and cell volume, nucleus/cytoplasm ratio, number and surface density, diameters and cell thickness. Mean body weight was 34.86 g for the control group and 18.56 g for the Cd-treated group. Histologically, the glandular acini were significantly smaller, the gland ducts were similar in both groups studied. The connective tissue was more abundant. In conclusion, the salivary glands (submandibular, parotid and sublingual) showed retarded growth after Cd intoxication.

Fluctuating abundance of humpback whales (Megaptera novaeangliae) in a calving ground off coastal Brazil

The humpback whale (Megaptera novaeangliae) population that uses Abrolhos Bank, off the east coast of Brazil as a breeding ground is increasing. To describe temporal changes in the relative abundance of humpback whales around Abrolhos, seven years (1998-2004) of whale count data were collected during July through to November. During one-hour-scans, observers determined group size within 9.3 km (5 n.m.) of a land-based observing station. A total Of 930 scans, comprising 7996 sightings of adults and 2044 calves were analysed using generalized linear models that included variables for time of day, day of the season, years and two-way interactions as possible predictors. The pattern observed was the gradual build-up and decline in whale counts within seasons. Patterns and peaks of adult and calf counts varied among years. Although fluctuation was observed, there was generally an increasing trend in adult counts among years. Calf counts increased only in 2004. These fluctuations may have been caused by some environmental conditions in humpback whales` summering grounds and also by changes in spatial-temporal concentrations in Abrolhos Bank. The general pattern observed within the study area mirrored what was observed in the whole Abrolhos Bank. Knowledge of the consistency with which humpback whales use this important nursing area should prove beneficial for designing future monitoring programmes especially related to whale watching activities around Abrolhos Archipelago.

Ultrastructural Aspects of Female Aging Wistar Rat Epithelium Tongue: A HRSEM and TEM Study

Background: There is general consensus that the effects of intrinsic aging on the oral mucosa are relatively small, though potentially important to understanding the pathologies present in the aged animals. Objective: In this paper, the development of dorsal surface of rat tongue was examined using transmission electron microscopy (TEM) and high-resolution scanning electron microscopy (HRSEM) in order to understand the age-related structural and ultrastructural changes experimentally. Methods: In this study, we used female rats 75 and 720 days old (adult and aging). Tissues of rat tongue were prepared and the specimens submitted to HRSEM and TEM techniques. Results: The analysis of HRSEM and TEM demonstrated that the same characteristic keratinous epithelium was found in aging animals, however with some modifications. Conclusion: We agree that there are obvious changes in the oral mucosa with aging and these modifications can be observed starting from the ultrastructural aspects. Copyright (C) 2009 S. Karger AG, Basel

Catalytic mechanism of inulinase from Arthrobacter sp S37

Detailed catalytic roles of the conserved Glu323, Asp460, and Glu519 of Arthrobacter sp. S37 inulinase (EnIA), a member of the glycoside hydrolase family 32, were investigated by site-directed mutagenesis and pH-dependence studies of the enzyme efficiency and homology modeling were carried out for EnIA and for D460E mutant. The enzyme efficiency (k(cat)/K-m) of the E323A and E519A mutants was significantly lower than that of the wild-type due to a substantial decrease in k(cat), but not due to variations in K-m, consistent with their putative roles as nucleophile and acid/base catalyst, respectively. The D460A mutant was totally inactive, whereas the D460E and D460N mutants were active to some extent, revealing Asp460 as a catalytic residue and demonstrating that the presence of a carboxylate group in this position is a prerequisite for catalysis. The pH-dependence studies indicated that the pK(a) of the acid/base catalyst decreased from 9.2 for the wild-type enzyme to 7.0 for the D460E mutant, implicating Asp460 as the residue that interacts with the acid/base catalyst Glu519 and elevates its pK(a). Homology modeling and molecular dynamics simulation of the wild-type enzyme and the D460E mutant shed light on the structural roles of Glu323, Asp460, and Glu519 in the catalytic activity of the enzyme. (C) 2008 Elsevier Inc. All rights reserved.

Landsbygdens lärjungar : En studie av den sociala rekryteringen vid realskolan i Rättvik 1930-1959

Syftet med denna uppsats är att undersöka den sociala bakgrunden hos elever som läste vid Realskolan i Rättvik mellan åren 1930 och 1959. Källmaterialet består av antagnings- och avgångsmatriklar från Realskolan i Rättvik där uppgifter om elevens kön, faderns yrke, eventuell examen samt om eleven valt att läsa vidare på gymnasium efter avslutad skolgång hämtats. Ovanstående insamlad data visar vilken socialgrupp och vilket kön eleverna hade som läste på skolan, vilka som avbröt sina studier i förtid samt vilka som läste vidare på gymnasium efter realexamen. Indelningen i socialgrupper har gjorts utifrån SCB’s socioekonomiska indelning, SEI. Resultatet visar att elevernas sociala bakgrund har varierat över tid och även varierat mellan könen. Resultatet visar även att denna variation stämmer överens med omvärldens förändringar, där samhälleliga och social förändringar avspeglar sig i resultatet. Resultatet visar att de största variationerna återfinns i de lägre socialgrupperna, socialgrupp 2 och 3, där hemmansägarnas och arbetarnas barn befinner sig.

Managing Seasonality in Tourism : Challenges and Opportunities for the Tourism Industry in Húsavík, Iceland

Seasonality is a common phenomenon in the tourism industry around the world. Húsavík, a town on the northern coast of Iceland, has experienced tremendous growth in tourism in recent years and is sometimes recognised as the „Whale Watching Capital of Europe‟. However, Húsavík faces extreme seasonality with high demand mainly during the summer months and limited or no demand at other times of the year. As is emphasised in the tourism literature and widely in practice, seasonality is frequently seen as a problem for the tourism industry which has to be tackled. Academic research has never before been done on seasonality in Húsavík and the aim of this thesis is to understand the nature of seasonality within the tourism industry in Húsavík and discover potential ways to reduce seasonality in Húsavík tourism. In order to fulfil the aim, qualitative semi-structured interviews were undertaken with six tourism managers in Húsavík to investigate their perceptions of seasonality and if and how it could be managed. The results confirmed that the peripheral location of the destination stimulates seasonality and it poses major economic challenges for tourism businesses in Húsavík. Managers would prefer a longer tourism season. Several recommendations were provided for how to reduce seasonality such as develop other attractions than whale watching, improve accessibility, focus marketing on seasons outside the summer, and offer winter packages for foreign and domestic tourists.

Power outage, and other poems

Contents - At the Starry Night Cafe AIbino Buffalo Monteverdi In The Rain Lust Litter Poem After The Chinese Cleere's Pub Treasure Island Nipple Periwinkle Talkeetna Beached Whale Spending Christmas in the land of Enchantment Easter Poem For Emily Dickinson Second Hand Gifts The Power Outage October In The Observation Car Westerly

Administração dos recursos de informática pública : estudo de caso do modelo paranaense

Este trabalho analisa as vantagens que o setor público pode obter da informatização. da Tecnologia da Informação (TI). Defende o estabelecimento de um modelo de administração de informática para garantir a gestão adequada dos recursos e apresenta uma sugestão de quais elementos devem ser contemplados na formulação de um modelo de gestão dos recursos de informática, na dimensão de uso da TI como ferramenta de administração. Estuda o caso do modelo de informática pública implantado no estado do Paraná - O SEI, Sistema Estadual de Informações, fazendo uma análise contra os elementos definidos anteriormente

Comportamento do consumidor online: perfil, uso da internet e atitudes

O trabalho tem como objetivo geral buscar um melhor entendimento do comportamento de compra online e, para tal, valeu-se de cinco objetivos específicos. O primeiro objetivo tratou da ordenação e sistematização do conhecimento sobre esse assunto e, através de seu atingimento, foi possível perceber que o comportamento de compras online tem sido explicado na literatura científica por 3 grandes grupos de variáveis: perfil do consumidor, tipo de uso que faz da Internet e atitude em relação a essa mídia. Também foi possível reduzir de 28 para 13 as variáveis relevantes na explicação desse tipo de consumo. O segundo objetivo tratou de investigar as características que diferenciam compradores de não-compradores e aqui foi possível identificar que compradores online têm perfil socioeconômico mais elevado, viajaram mais para o exterior, conhecem mais o idioma inglês e recebem salários mais altos. Também valorizam mais a conveniência, são mais inovadores, têm menor aversão ao risco e uma orientação experiencial menor, ou seja, necessitam menos ver e pegar o produto antes de decidir comprar. Esses consumidores também têm mais locais de acesso à rede, consideram ter maior conhecimento sobre a Web e a utilizam mais para e-mails, operações bancárias e para levantar informações sobre produtos e serviços. Tendem a enxergar a Internet mais útil e divertida do que os não-compradores. O terceiro objetivo tratou de identificar diferentes segmentos de consumidores online e 5 grupos distintos surgiram. Entre os não-compradores caracterizou-se os grupos Internet não é comigo, Gosto da Internet, mas não sei se vou comprar e o Estou quase lá. Entre os compradores foi possível encontrar os grupos Estou testando e Fãs de carteirinha. Percebeu-se que os cinco grupos formam um contínuo que representa um caminhar em direção às compras online, guardando semelhanças com a Teoria da Difusão das Inovações. O quarto objetivo buscou desenvolver um modelo que estimasse como a probabilidade de consumo online é afetada pelo perfil, uso da Internet e atitudes. Segundo esse modelo, tal probabilidade é maior quando o consumidor acessa a Internet de mais lugares; utiliza a rede para banking, levantar informações sobre produtos e serviços e buscar ofertas; viaja mais para o exterior; conhece mais o idioma inglês; possui mais bens digitais; está mais envolvido com a Internet; vê mais utilidade da na rede; acha importante poder comprar sem sair de casa; tem mais propensão ao risco; não se julga muito inovador; e, mais importante, tem baixa orientação experiencial, dispondo-se a comprar sem ver e pegar o produto antes. O quinto objetivo era oferecer sugestões de cunho prático a gerentes e empreendedores e entre elas é possível destacar as seguintes: 1) Constatou-se a necessidade dos sites também terem elementos hedônicos, não apenas utilitários; 2) Os produtos de mais aceitação para venda online são os de busca, seguidos, na ordem, pelos tipo experiência-2, tipo experiência-1 e, por último, confiança; 3) Uma maior percepção de segurança online leva a mais compras e maior freqüência de compras; 4) Os achados descritos no quarto objetivo podem servir para localizar potenciais consumidores online; 5) As descobertas do terceiro objetivo podem orientar o processo de conversão e manutenção de compradores online.

Limites e possibilidades da gestão sob o enfoque holístico

O tema Gestão sob o enfoque holístico surgiu a partir de uma leitura da revista HSM Management de setembro-outubro/2000. O artigo abordava o novo paradigma de gerenciar funcionários, que privilegia a busca do bem-estar, onde muitas empresas estão sendo obrigadas a revisar suas políticas de recursos humanos para enfocar as três dimensões de um indivíduo - corpo, mente e espírito. Segundo a reportagem, pesquisas da PricewaterhouseCoopers, Universum e outras organizações constataram que o equilíbrio trabalho/vida não é apenas uma demanda dos atuais empregados, mas também uma das principais prioridades dos formandos em universidades do mundo todo. A intensa luta para atrair funcionários de alto gabarito colocará a empresa com abordagem mais holística um degrau acima de suas concorrentes. Para as empresas, atender as verdadeiras necessidades individuais de seus funcionários é um novo paradigma na maneira de gerenciar seus negócios. O que mudou é a percepção de que o ser humano é fator primordial de competitividade e deixou de ser insumo. O ser humano passou a ser tendencialmente o centro, o foco das relações. Ainda que este conceito aparente estar longe da realidade organizacional brasileira, em função da distância entre o discurso e a prática de um modelo de gestão que considere o funcionário como um ser integral, já temos exemplos de empresas que estão adotando este princípio. A necessidade do equilíbrio entre os aspectos físico, mental, emocional e espiritual para ser feliz, tem levado as pessoas a superarem o medo do desconhecido e caminhar com detenninação em direção ao autodesenvolvimento. Este estudo analisou a utilização da gestão sob o enfoque holístico em duas diferentes empresas. Os resultados evidenciaram que a abordagem holística não está no âmbito da utopia, antes trata-se de um desafio para os gestores das organizações que, para um sucesso na adoção desta prática, precisam, mais do que implantá-la, adotá-la como filosofia de vida, incorporando-a a seus valores e visão de mundo. A prática cotidiana do modelo de gestão holística a partir do corpo diretivo é que vai possibilitar a verdadeira transformação da cultura organizacional tradicional para uma cultura organizacional holística, cuja conseqüência é o reflexo positivo nos resultados econômico-financeiros. C onstatamos, por outro lado, as limitações na utilização desse modelo de gestão tanto por parte dos funcionários quanto por parte dos altos executivos. Quanto aos funcionários, nem todos estão preparados para assumir novas responsabilidades ou preparados para o seu desenvolvimento pessoal, através do auto conhecimento. As crenças adquiridas no período educacional, tanto no lar quanto na escola, bem como suas recentes experiências profissionais podem inibir a descoberta de novas possibilidades, até que se sintam compelidos a lidar com os novos conhecimentos por necessidade de empregabilidade. Quanto aos executivos, por se tratar de um modelo de gestão relativamente novo, sem comprovação científica aliado à dificuldade de encontrar instrumentos de medição objetivos e ao elevado nível de exigência quanto a resultados crescentes no curto prazo, acabam priorizando a administração por objetivos, por se sentirem mais confortáveis quanto à certeza de obtenção de resultados econômico-financeiros no curto prazo. Esquecem-se, no entanto, dos efeitos desastrosos no clima organizacional, que vão comprometer os resultados de médio e longo prazos, seja pela possibilidade de elevado turnover como pela desmotivação generalizada, onde os funcionários só trabalham sob pressão. Por sua vez, cientes da importância do ser humano no novo cenário de elevada competitividade, não são poucos os empresários que já estão começando a trabalhar seus valores intrinsecos com a ajuda de terapeutas, filósofos, psicólogos, estimulando primeiro o auto conhecimento pessoal, para que possam futuramente empreender essa nova maneira de ver o mundo em suas respectivas empresas.

Neue medien und text: privatbrief und private E-Mail im vergleich

Die vorliegende Dissertation setzt sich mit dem Phänomen ›Text im Rahmen der Neuen Medien‹ auseinander, indem sie theoretisch und empirisch der in der einschlägigen Forschung aufgeworfenen (und kontrovers diskutierten) Frage nachgeht, ob die Sprache in den Textsorten der neuen Kommunikationsformen als neue Schriftlichkeit bzw. schriftliche Mündlichkeit zu verstehen sei. Dabei konzentriert sie sich exemplarisch auf die Analyse eigens für diesen Zweck erstellter aktueller Textkorpora der Kommunikationsformen E-Mail und Brief und untersucht sie unter den Gesichtspunkten Mündlichkeit / Schriftlichkeit bzw. Nähe / Distanz. ›Kapitel 1‹ umreißt den Forschungsstand zum Thema ›Text und Textsorte‹ sowohl in der Textlinguistik als auch in aktuellen Studien zur Sprache in den Neuen Medien. ›Kapitel 2‹ ist dem kritischen Referat verschiedener bekannter Modelle gewidmet, die sich mit dem Aspekt der Mündlichkeit und Schriftlichkeit bzw. Nähe und Distanz beschäftigen (Hugo Stegers Freiburger Gruppe, Ludwig Söll, Koch/Oesterreicher und Ágel/Hennig) und eine Verortung von Textsorten im Kontinuum Nähe und Distanz vornehmen (Koch/Oesterreicher und Ágel/Hennig).›Kapitel 3‹ schlägt Korrekturen und Ergänzungen betreffend Punktgebung, Modellglossar und Makroanalyse im Modell von Ágel/Hennig vor, das in dieser Arbeit den Analysen der Nähesprachlichkeit in Privatbriefen und privaten E-Mails zugrunde liegt. ›Kapitel 4‹ setzt sich mit Aspekten der beiden Textkorpora auseinander, auf die sich diese Arbeit stützt, und beschreibt und erläutert den Fragebogen, der im Rahmen dieser Arbeit erstellt und Probanden zur Beantwortung vorgelegt wurde. ›Kapitel 5‹ nimmt einen ausführlichen Vergleich der Textsorten ›Privatbrief und private E-Mail‹ vor und beleuchtet abrissartig die Geschichte beider Kommunikationsformen, wobei es sich gleichzeitig auch kritisch mit den in der Forschung in solchen Vergleichsfragen vertretenen Positionen auseinander setzt. ›Kapitel 6‹ interpretiert die Ergebnisse der in der Arbeit durchgeführten Näheanalysen und zieht daraus die Schlussfolgerungen.

Population structure, habitat use and conservation of short-finned pilot whales Globicephala macrorhynchus in the Archipelago of Madeira

This thesis provides information on the grouping structure, survival, abundance, dive characteristics and habitat preferences of short-finned pilot whales occurring in the oceanic archipelago of Madeira (Portugal, NE Atlantic), based on data collected between 2001-2011, and contributes for its conservation. Photo-identification methods and genetic analyses demonstrated that there is a large degree of variability in site fidelity, including resident, regular visitor and transient whales, and that they may not be genetically isolated. It is proposed that the pilot whales encountered in Madeira belong to a single population encompassing several clans, possibly three clans of island-associated (i.e. resident and regular visitor) whales and others of transients, each containing two to three matrilineal pods. Mark-recapture methods estimated that the island-associated community is composed of less than 150 individuals and that their survival rate is within the range of other long-lived cetacean species, and that around 300 whales of different residency patterns uses the southern area of the island of Madeira from mid-summer to mid-autumn. No significant trend was observed between years. Time-depth recorders deployed in adult whales during daytime revealed that they spend over ¾ of their time at the surface, that they have a low diving rate, and that transient whales also forage during their passage. The analyses of visual data collected from nautical and aerial line-transect surveys indicate a core/preferred habitat area in the south-east of the island of Madeira. That area is used for resting, socializing, foraging, breeding, calving and birthing. Thus, that area should be considered as an important habitat for this species, at least seasonally (during autumn) when the species is more abundant, and included in conservation plans. No direct threat needing urgent measures was identified, although the impact of some activities like whale-watching or marine traffic should be assessed.

Purificação e caracterização de uma β-glucuronidase extraída do molusco mesogastropoda ampularidade Pomacea sp

This work studies the involved enzymatic way in the metabolism of glycosaminoglycans sulfateds in the mollusc Pomacea sp. Had been identified endoglycosidases and exoglycosidases in the enzymatic extract of the mollusc Pomacea sp by means of hydrolysis activity in condroitim sulphate of whale cartilage and of the p-Nitrofenil-β-glucuronide, respectively. The enzymatic extracts qere obtained of Pomacea sp. being used of 0.1 sodium acetate buffer, pH 5.0 and later centrifugated the 8,000 x g and the presents proteins in the sobrenadante were submitted to the fractionament with two crescents ammonium sulphate concentrations, the visualized activity biggest in the F2 fraction (50-80%). The β-glucuronidase (F3) was isolated in gel chromatography filtration Biogel 1.5m, the purification degree was ratified in Chromatography Liquid of high efficiency (HPLC). The enzyme was purificated 6.362,5 times with 35,6% yield. The β -glucuronidase isolated in this work showed a molecular mass of 100 kDa, determined for eletroforese in poliacrilamida gel . The determination of the ideal kinetic parameters for the catalysis of the p-nitrofenil- β -glucuronide for β-glucuronidase, showed excellent activity in pH 5,0 and temperature 65ºC for 6 hours and apparent Km of 72 x 10-2 mM. It is necessary for the total degradation of 3mM of p-N-β-glucoronide, the amount of 1,2μg of ss-glucuronidase. The BaCl2 increased the activity of ss-glucuronidase, and the activity was inhibited completely by the composites SDS and NaH2PO4

Educação em saúde em hanseníase: estratégia na educação básica, Parnamirim/RN

Leprosy is an ancient disease that still stands as a public health problem worldwide, especially in the considered developing countries. Of these, Brazil still has large areas of endemicity. The disease remains high among those younger than 15 years old. In this group, the national index achieves 0.6 every 10 000 inhabitants and reaches 2 in the North and Midwest of Brazil. Therefore, the plan of the Ministry of Health is to prioritize the fight against disease in critical areas, providing early diagnosis and timely treatment, especially for patients under 15 years old. This research aimed to: identify the knowledge of students from three schools of basic education in Parnamirim/RN about the risk of getting ill in leprosy; conduct them health education on this topic. An exploratory-descriptive study with quantitative and qualitative nature. Data collection consists of two steps: questionnaire and health education on leprosy. The study was approved by the Research Ethics Committee (REC) of UFRN through the opinion nº 204/2009. Quantitative data obtained were organized, categorized, typed and submitted to the Microsoft Office - Excel for quantitative analysis with simple percentage. Qualitative data were subjected to a content analysis according to Bardin. The sample consisted of 164 students of basic education, most of which is: 1st year of high school, from 11 to 15 years, in the afternoon shift, female gender, skin color white and residents in the municipality of Parnamirim/RN. Still, each student living with four people curiously did not respond in relation to occupation of the father. But the same answered their mother as a housewife and a family income of one to less than four minimum wages. There have been significant results compared the performance of health education in leprosy. Initially, students possessed little knowledge about leprosy. Subsequent to health education, most knew the answer about the disease, transmission, cause, where to get help in case of suspicion of leprosy and the need for the people who lives with leprosy patients to also seek for help, the understanding about if it is treatable or not. Content analysis has established the following themes: axis I, what I do not know about leprosy: cure, fear and prejudice. Axis II, what I know about leprosy, we identified: cure, effectiveness of health education and social exclusion in the past. It is considered the scope of the proposed objectives by matching the effectiveness of health education on leprosy in basic education in Parnamirim/RN; emphasizes, therefore, the importance of understanding the knowledge of public school students. It is expected of them to detect early cases of leprosy in their communities; encourage adherence to treatment as quickly as possible; prevent disability; reduce the stigma surrounding the disease.