961 resultados para Carboxyl-terminal Fragment
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Trabalho Final de Mestrado para obtenção do grau de Mestrado em Engenharia Electrónica e Telecomunicações
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The synthetic study of the uppermost Cretaceous of the Beira Litoral (fauna, floral confirms its upper Campanian-Maastrichtian age. It shows the presence of a tropical to subtropical climate in an area constituted by a low coastal plain only occasionally linked to the sea, saturared with fresh water and possessing accordingly, a predominantely freshwater fauna (Viso, Aveiro); this plain changed towards the interior into a drier more forested zone with a more abundant terrestrial fauna which includes mammals (Taveiro). A thorough study of the chelonian Rosasia, abundant on the coastal plain, was made possible thanks to the discovery of a skull: it demonstrates that the genus belongs to the family Bothremydidae, revalided here. The composition of this family is presented, its phylogenetic and paleobiogeographic relation with the other pleurodires are analyzed, and its diagnosis established. The family is constituted of three groups; Rosasia belongs to one of rhese, the Bothremys group.
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The reef levels of the gulf of skoura belong to the reef formation of the Upper Miocene of the South-Rifan straits. A detailed analysis of the vertical distribution of various forms of colonies has led to the establishment of precise coral morphologies zonation. This palaeoecological approach leads us to distinguish between two environments in the Skoura gulf, probably corresponding to two reef episodes. The palaeogeographical implications (relationships between the Atlantic ocean and the Mediterranean sea) will be pointed out.
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A rich uppermost Miocene selachian fauna from the Alvalade Basin (represented by more than 10.000 teeth) is accounted for. It is the most modern miocene fauna of neritic habit under warm-temperate to subtropical conditions, known in the european Miocene.
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An Upper Miocene important sedimentary break can be accurately recognised in the Portuguese basins and is reflected by a drastic palaeogeographic change in relation to a large-scale tectonic event of probable uppermost Vallesian-Turolian (9,5 Ma; middle Tortonian) age. The characterisation of the sedimentary record of this tectonic event, as well as its relations with interpreted active faults is made for different situations: Douro (NW border), Mondego, Lower-Tagus and Sado Tertiary basins. The sedimentary record, considered upper Tortonian-Messinian ? (uppermost Vallesian-Turolian ?) is interpreted mainly as endorheic alluvial fans (internal drainage), developed along active NNE-SSW indent-linked strike-slip faults and NE-SW reverse faults. At NE Portugal, proximal fluvial systems of an endorheic hydrographic network drained eastwards to the Spanish Duero interior Basin. The main evidences of the betic compression clímax in Portugal mainland are presented; the interpreted active tectonic structures are in accordance with an intense NNW-SSE crustal shortening, but some regional differences are also documented.
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Dissertação de Mestrado em Engenharia Informática
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In the present study we report the results of an analysis, based on ribotyping of Corynebacterium diphtheriae intermedius strains isolated from a 9 years old child with clinical diphtheria and his 5 contacts. Quantitative analysis of RFLPs of rRNA was used to determine relatedness of these 7 C.diphtheriae strains providing support data in the diphtheria epidemiology. We have also tested those strains for toxigenicity in vitro by using the Elek's gel diffusion method and in vivo by using cell culture method on cultured monkey kidney cell (VERO cells). The hybridization results revealed that the 5 C.diphtheriae strains isolated from contacts and one isolated from the clinical case (nose case strain) had identical RFLP patterns with all 4 restriction endonucleases used, ribotype B. The genetic distance from this ribotype and ribotype A (throat case strain), that we initially assumed to be responsible for the illness of the patient, was of 0.450 showing poor genetic correlation among these two ribotypes. We found no significant differences concerned to the toxin production by using the cell culture method. In conclusion, the use of RFLPs of rRNA gene was successful in detecting minor differences in closely related toxigenic C.diphtheriae intermedius strains and providing information about genetic relationships among them.
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Dissertation presented to obtain the Ph.D degree in Developmental Biology
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The 15th International Conference on Runtime Verification (RV'15). 22-25 September. Vienna, Austria.
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Infraestrutura com mais de um século de história, o Porto de Leixões encontra-se em fase de renovação com a criação de um emblemático Edifício de Terminal de Cruzeiros. Obra de arquitetura arrojada, o edifício é implantado em condições geológico-geotécnicas adversas e na convivência com o efeito da água do mar e das respetivas marés. O presente relatório é relativo a um Estágio realizado na obra numa fase de execução dos trabalhos de fundações e de contenções periféricas (ensecadeira). Apresentam-se as soluções sugeridas em projeto, as soluções apresentadas inicialmente pelo construtor e as alternativas a estas, propostas já durante a obra. Descrevem-se as diferentes soluções, identificando os equipamentos, recursos humanos e procedimentos envolvidos. Comparam-se os respetivos rendimentos e custos. Apresenta-se o Plano de Instrumentação e de Monitorização implementado em obra. Descreve-se em pormenor o trabalho realizado pelo estagiário, em obra, no decurso do estágio.
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Dissertação apresentada para cumprimento dos requisitos necessários à obtenção do grau de Mestre em História Contemporânea
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Os ependimomas são tumores sólidos com origem nas células que delimitam o sistema ventricular e o canal ependimário. A sua localização habitual varia com a idade e, nos ependimomas espinhais, a sintomatologia é inespecífica o que motiva diagnósticos tardios. Os autores descrevem o caso clínico de uma criança de 9 anos de idade, com dor lombosagrada, alteração da marcha, postura anti-álgica e inversão da lordose lombar. A ressonância magnética nuclear (RMN) revelou um -tumor da cauda equina (filum terminal). Procedeu-se à excisão completa do tumor; o exame histológico permitiu diagnosticar um ependimoma mixo-papilar do filum terminal. No pós-operatório verificou-se retenção urinária transitória que resolveu gradualmente. Dois anos após a cirurgia o doente encontra-se assintomático persistindo apenas ausência dos reflexos aquilianos. Faz-se uma pequena abordagem das manifestações clínicas, diagnóstico e terapêutica dos ependimomas espinhais.
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Dissertação para obtenção do Grau de Mestre em Engenharia Civil
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Fluids in which Mycobacterium tuberculosis are seldom found, such as pleural and cerebrospinal liquids, are good candidates to be studied using PCR techniques. We detail our experience with a PCR assay applied to pleural and cerebrospinal fluids using the primer MPB64. Seventy three specimens were analyzed: 30 pleural fluids (PF), 26 pleural biopsies (PB) and 17 cerebrospinal fluids (CSF). The gold standard for the diagnosis of tuberculous meningitis was the positive culture for M. tuberculosis in CSF. Tuberculous pleural effusion was diagnosed when cultures of PF and/or PB were positive for M. tuberculosis, or the PB histology showed granulomas. Our results, compared to the gold standards employed, showed a sensitivity of 70%, specificity of 88%, positive predictive value of 82% and negative predictive value of 80%. The high specificity of the MPB64 fragment while still retaining a good sensitivity makes it very well suited for pleural and cerebrospinal tuberculosis diagnosis.
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We sequenced the internal transcribed spacer 2 of the ribosomal DNA (ITS2-DNAr) from the three Schistosoma mansoni intermediate hosts in Brazil: Biomphalaria glabrata, Biomphalaria tenagophila and Biomphalaria straminea. Analysis of a restriction map from those sequences allowed us to select putative restriction enzymes able to identify the snail species under study. Four restriction enzymes were used and HpaII provided simple species-specific profiles easily visualized in polyacrylamide gels. The use of ITS2 is advantageous as it provides a small fragment of 460 bp which may be easily amplified by PCR. In the current work, we showed that the amplification of ITS2-DNAr together with HpaII enzyme restriction is an auxiliary molecular tool for the morphological identification of such snails as well as for taxonomic and phylogenetic studies of neotropical planorbids.