Changes in the aroma of organic passion fruit (Passiflora edulis Sims f. flavicarpa Deg.) during ripeness

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Structural characterization of an etheno-2 '-deoxyguanosine adduct modified by tetrahydrofuran

The reaction of 2'-deoxyguanosine with the alpha,beta-unsaturated aldehydes trans-2-octenal, trans-2-nonenal, trans-2-decenal, trans,trans-2,4-nonadienal, and trans,trans-2,4-decadienal in THF gives rise to three novel adducts: 3-(2'-deoxy-beta-D-erythro-pentafuranosyl)-7-[3-hydroxy-1-(3(2'-deoxy-beta-D-erythro-pentafuranosyl)-3,5-dihydro-imidazo[1,2-alpha]purin-9-one-7-yl)-propyl] -3,5-dihydro-imidazo[1,2-alpha]purin-9-one (M) and 3-(2'-deoxy-beta-D-erythro-pentafuranosyl)-7-(tetrahydrofuran-2-yl)-3,5-dihydro-imidazo[1,2-alpha]purin-9-one (A8 and A9), which are not observed in the absence of THF. These adducts were isolated from in vitro reactions by reversed-phase HPLC and fully characterized on the basis of spectroscopic measurements. Adduct A7 consists of two 1,N-2-etheno-2'-deoxyguanosine (1,N-2-epsilondGuo) residues linked to a hydroxy-carbon side chain; adducts A8 and A9 are interconvertible 1,N-2-epsilondGuo derivatives bearing a THF moiety. The proposed reaction mechanism involves the electrophilic attack on 1,N-2-epsilondGuo by the carbonyl of 4-hydroxy-butanal, generated via ring opening of alpha-hydroxy-THF (THF-OH), yielding adducts A8 and A9. A further combination of these adducts with another 1,N-2-epsilondGuo produces the double adduct A7. These findings demonstrate that reactions of unsaturated aldehydes in the presence of THF produce novel condensation 1,N-2-epsilondGuo-THF adducts. Further studies would indicate the relevance of these adducts in THF toxicity.

Improvement of Pro-Oxidant Capacity of Protocatechuic Acid by Esterification

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of modified silica nanoparticles in carboxylated nitrile rubber nanocomposites

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção e caracterização das Tanases do fungo filamentoso Aspergillus carbonarius

Pós-graduação em Biotecnologia - IQ

Materiais híbridos argila-poli(metil metacrilato) obtidos através do método grafting

Pós-graduação em Química - IQ

Estudo fitoquímico de extratos polares e infusão das folhas de Terminalia catappa L. (Combretaceae) e avaliação de suas atividades antiulcerogênica e mutagênica

Pós-graduação em Química - IQ

Involvement of alpha(1B)-adrenoceptors in the anti-immobility effect of imipramine in the tail suspension test

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Antibacterial activity of alkyl gallates is a combination of direct targeting of FtsZ and permeabilization of bacterial membranes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Study on determination and removal of metallic ions from aqueous and alcoholic solutions using a new POSS adsorbent

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Contrasting effects of nitric oxide and corticotropin-releasing factor within the dorsal periaqueductal gray on defensive behavior and nociception in mice

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo dos mecanismos de resistência e virulência de isolados de Cryptococcus neoformans e Cryptococcus gattii e quantificação de genes de bomba de efluxo pós tratamento com Galatos de Alquila

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of byrosima crassa and phenolic constituents on helicobacter pylori: induced neutrophils oxdative burst

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Toxicity and loss of mitochondrial membrane potential induced by Alkyl Gallates in Trypanosoma cruzi

American trypanosomiasis or Chagas disease is a debilitating disease representing an important social problem that affects, approximately, 10 million people in the world. The main aggravating factor of this situation is the lack of an effective drug to treat the different stages of this disease. In this context, the search for trypanocidal substances isolated from plants, synthetic or semi synthetic molecules, is an important strategy. Here, the trypanocidal potential of gallates was assayed in epimastigotes forms of T. cruzi and also, the interference of these substances on the mitochondrial membrane potential of the parasites was assessed, allowing the study of the mechanism of action of the gallates in the T. cruzi organisms. Regarding the preliminary structure-activity relationships, the side chain length of gallates plays crucial role for activity. Nonyl, decyl, undecyl, and dodecyl gallates showed potent antitrypanosomal effect (IC50 from 1.46 to 2.90 μM) in contrast with benznidazole (IC50 = 34.0 μM). Heptyl gallate showed a strong synergistic activity with benznidazole, reducing by 105-fold the IC50 of benznidazole. Loss of mitochondrial membrane potential induced by these esters was revealed. Tetradecyl gallate induced a loss of 53% of the mitochondrial membrane potential, at IC50 value.

Effect of silica surface on luminescence properties of tb3+-glutamic acid.

We studied the effect of silica surface on luminescence properties of terbium complex by spectroscopy characterization, where microparticles of mesoporous silica type MSU-X was prepared. We used silica with different surface: calcined, washed, functionalized with 3- aminopropyl-triethoxysilane (APTES), and 3-glycidoxypropyl-trimethoxysilane (GPTMS); impregnated with Tb3+-glutamic acid complex. The obtained materials were characterized by scanning electron microscopy, porosity measurements, small-angle X-ray scattering, as structural characterization; Fourier transform infrared and luminescence spectroscopy, as spectroscopy characterization. Finally, we observed that functional groups at the silica surface lead to changes on luminescent properties of the final materials. The observed shift of the absorption and emission bands can be assigned to the effect of the functional groups of mesoporous silica.