Prenylcysteine α-Carboxyl Methyltransferase in Suspension-Cultured Tobacco Cells1

Isoprenylation is a posttranslational modification that is believed to be necessary, but not sufficient, for the efficient association of numerous eukaryotic cell proteins with membranes. Additional modifications have been shown to be required for proper intracellular targeting and function of certain isoprenylated proteins in mammalian and yeast cells. Although protein isoprenylation has been demonstrated in plants, postisoprenylation processing of plant proteins has not been described. Here we demonstrate that cultured tobacco (Nicotiana tabacum cv Bright Yellow-2) cells contain farnesylcysteine and geranylgeranylcysteine α-carboxyl methyltransferase activities with apparent Michaelis constants of 73 and 21 μm for N-acetyl-S-trans,trans-farnesyl-l-cysteine and N-acetyl-S-all-trans-geranylgeranyl-l-cysteine, respectively. Furthermore, competition analysis indicates that the same enzyme is responsible for both activities. These results suggest that α-carboxyl methylation is a step in the maturation of isoprenylated proteins in plants.

Plan de estrategias para mejorar la participación de Geomedicos y posicionamiento de la categoría

Geomedicos S.A.S identifica mayor cantidad de servicios a sus clientes generando mayor valor agregado, logrando fidelización y canaliza las necesidades de su cliente potencial, basado en buenos estándares de calidad; representado en un buen servicio. Adoptando el siguiente paradigma “la calidad de un producto o servicio conduce a la satisfacción del cliente que lleva a la fidelización del cliente, que conduce a la rentabilidad. La participación de todo un grupo de trabajo orientados a dar las mejor resultados, es lo que más motiva para hacer que la empresa de logros positivos y óptimos de acuerdo a un plan estratégico que se hace interno y productivo para que la gerencia tome decisiones acordes al crecimiento y ponderación; determinando incentivos que favorezcan la calidad de vida de cada uno de sus empleados y de sus familias, haciendo partícipe a cada uno de ellos.

Estágio de natação na equipa de competição da Associação Naval Amorense

Este relatório surge no âmbito do Mestrado em Treino Desportivo, pretendendo dar a conhecer as minhas aprendizagens e trabalho desenvolvido durante o estágio de carater profissionalizante realizado com a equipa de natação absoluta da Associação Naval Amorense ao longo da época 2014/2016. Este estágio permitiu-me analisar e intervir em diversos aspetos integrados no processo de treino da modalidade de natação, tais como: a análise e correção técnica, análise do planeamento da época desportiva e intervenção, tanto em contexto de competição como também em tarefas das sessões de treino. Desta forma, foi-me possível não só obter um conhecimento mais abrangente e real dos processos de treino, mas também, o enquadramento da informação e conhecimento que foram fornecidos ao longo de todo o meu percurso académico. Foi ainda possível acrescentar uma componente de investigação a este mesmo relatório, através de um projeto cientifico realizado com alguns dos nadadores de referência do clube.

Application of spatial analysis techniques to adjust for fertility trends and identify interplot competition in early stage sugarcane selection trials

Most sugarcane breeding programs in Australia use large unreplicated trials to evaluate clones in the early stages of selection. Commercial varieties that are replicated provide a method of local control of soil fertility. Although such methods may be useful in detecting broad trends in the field, variation often occurs on a much smaller scale. Methods such as spatial analysis adjust a plot for variability by using information from immediate neighbours. These techniques are routinely used to analyse cereal data in Australia and have resulted in increased accuracy and precision in the estimates of variety effects. In this paper, spatial analyses in which the variability is decomposed into local, natural, and extraneous components are applied to early selection trials in sugarcane. Interplot competition in cane yield and trend in sugar content were substantial in many of the trials and there were often large differences in the selections between the spatial and current method used by the Bureau of Sugar Experiment Stations. A joint modelling approach for tonnes sugar per hectare in response to fertility trends and interplot competition is recommended.

Vertical competition and multi-period interaction : a theoretical and behavioral analysis on the impact of strategic inventories in supply chain management

Otto-von-Guericke-Universtität Magdeburg, Fakultät für Wirtschaftswissenschaft, Univ., Dissertation, 2015

Competition with forward contracts: a laboratory analysis motivated by electricity market design

We use experiments to study the efficiency effects for a market as a whole of adding the possibility of forward contracting to a pre-existing spot market. We deal separately with the cases where spot market competition is in quantities and where it is in supply functions. In both cases we compare the effect of adding a contract market with the introduction of an additional competitor, changing the market structure from a triopoly to a quadropoly. We find that, as theory suggests, for both types of competition the introduction of a forward market significantly lowers prices. The combination of supply function competition with a forward market leads to high efficiency levels.

Price competition under cost uncertainty: A laboratory analysis

We study the relation between the number of firms and price-cost margins under price competition with uncertainty about competitors' costs. We present results of an experiment in which two, three and four identical firms repeatedly interact in this environment. In line with the theoretical prediction, market prices decrease with the number of firms, but on average stay above marginal costs. Pricing is less aggressive in duopolies than in triopolies and tetrapolies. However, independently from the number of firms, pricing is more aggressive than in the theoretical equilibrium. Both the absolute and the relative surpluses increase with the number of firms. Total surplus is close to the equilibrium level, since enhanced consumer surplus through lower prices is counteracted by occasional displacements of the most efficient firm in production.

Party competition in regional elections: a framework for analysis

Whilst scholars have long recognised that processes of decentralisation create new regional arenas where distinct patterns of party competition are likely to emerge, there has been little systematic analysis of the dynamics of such competition. This working paper thus proposes a framework for analysing party competition between regional branches of state-wide parties, and autonomist parties, in regional arenas. Firstly, the different strategies political parties may adopt in response to their perceptions of voter preferences and to the strategies pursued by their competitors are identified. Secondly, different factors that impact on parties' strategic choices, and which may constrain a party's ability to select electorally optimal strategies in a given political context, are proposed.

Quality and Competition: An Empirical Analysis across Industries, March 2006

This paper empirically explores the link between quality and concentration in a cross-section of manufactured goods. Using concentration data and product quality indicators, an ordered probit estimation explores the impact of concentration on quality that is defined as an index of quality characteristics. The results demonstrate that market concentration and quality are positively correlated across different industries. When industry concentration increases, the likelihood of the product being higher quality increases and the likelihood of observing a lower quality decreases.

Transcriptome analysis of intraspecific competition in Arabidopsis thaliana reveals organ-specific signatures related to nutrient acquisition and general stress response pathways.

ABSTRACT: BACKGROUND: Plants are sessile and therefore have to perceive and adjust to changes in their environment. The presence of neighbours leads to a competitive situation where resources and space will be limited. Complex adaptive responses to such situation are poorly understood at the molecular level. RESULTS: Using microarrays, we analysed whole-genome expression changes in Arabidopsis thaliana plants subjected to intraspecific competition. The leaf and root transcriptome was strongly altered by competition. Differentially expressed genes were enriched in genes involved in nutrient deficiency (mainly N, P, K), perception of light quality, and responses to abiotic and biotic stresses. Interestingly, performance of the generalist insect Spodoptera littoralis on densely grown plants was significantly reduced, suggesting that plants under competition display enhanced resistance to herbivory. CONCLUSIONS: This study provides a comprehensive list of genes whose expression is affected by intraspecific competition in Arabidopsis. The outcome is a unique response that involves genes related to light, nutrient deficiency, abiotic stress, and defence responses.

Do brazilian states engage in the fiscal war of ports? An empiric study of tax competition and an analysis of a natural experiment: the brazilian senate resolution 13/2012

O fim do ano de 2014 marcou o segundo aniversário da Resolução 13/2012 (R13) do Senado brasileiro. Grosso modo, R13 constituiu-se de um normativo do Senado cujo objetivo era o de por um fim na Guerra Fiscal dos Portos (FWP), uma competição fiscal entre os estados que se dá através da concessão de benefícios fiscais sobre operações interestaduais com mercadorias importadas de modo a atrair empresas importadoras para o território do estado concedente. R13 diminuiu o nível da tributação sobre tais operações, esperando com isso diminuir os lucros auferidos e a propensão das firmas de aceitarem tais regimes especiais de incentivação fiscal. Nada obstante, R13 gerou uma grande discussão sobre se os benefícios da atração de investimentos para um estado em particular superariam ou não os custos que esse estado incorreria em renunciar receitas tributárias em razão concessão desses benefícios fiscais. O objetivo do presente trabalho é o de dar uma contribuição a essa discussão, testando se um comportamento de interação estratégica entre estados, tal como aquele que supostamente ocorre no contexto da FWP, de fato emerge dos dados de importação coletados de janeiro de 2010 a maio de 2015, e, também, testando se a R13 de fato afetou tal comportamento de interação estratégica. Utiliza-se aqui um modelo de econometria espacial, no qual se especifica uma matriz de pesos que agrega o nível de importação das jurisdições concorrentes, organizando os dados em um painel de efeitos fixos. Os resultados sugerem que existe um comportamento de interação estratégica entre os estados e que a R13 de fato impactou tal comportamento.

The analysis of the basketball coach speech during the moments of game and pause in relation to the performance in competition

In the area of the professional competition, the coach is a fundamental part in the management of a team and more concretely in the game planning. During the competition, the management of the times of pause and times out as well as the conduct of the coach during the same ones is an aspect to analyze in the sports performance. It is for this that it becomes necessary to know some of the behaviors that turn out to be more frequent by the coach and that are more related to a positive performance of his players. For it there has been realized a study of 7 cases of expert coaches in those that his verbal behavior has observed during 4 games. It has focused on the content of the information only to verbal level, on his meaning. The information that have been obtained in the study shows a major quantity of information elaborated during the pauses of the games and a major tactical content with regard to the moments of game. On the other hand, a relation exists between a major number of questions and a minor number of psychological instructions when the score is adverse, whereas in case of victory, a direct relation does not exist with any category. The rest of categories of the speech do not meet influenced directly for the result, for what it is not possible to consider a direct and immediate relation between the coach verbal behavior during the pauses and the result of the game, except in punctual moments.

Analysis of mode competition in a monolithic master- oscillator power‐amplifier emitting at 1.5 μm

The optical and radio-frequency spectra of a monolithic master-oscillator power-amplifier emitting at 1.5 ?m have been analyzed in a wide range of steady-state injection conditions. The analysis of the spectral maps reveals that, under low injection current of the master oscillator, the device operates in two essentially different operation modes depending on the current injected into the amplifier section. The regular operation mode with predominance of the master oscillator alternates with lasing of the compound cavity modes allowed by the residual reflectance of the amplifier front facet. The quasi-periodic occurrence of these two regimes as a function of the amplifier current has been consistently interpreted in terms of a thermally tuned competition between the modes of the master oscillator and the compound cavity modes.

Uso del cálculo numérico CFD para el estudio de actuaciones de una motocicleta de competición = CFD analysis of a competition motorbike aerodynamic performance

Esta memoria pretende ilustrar el desarrollo del Proyecto de Fin de Carrera Uso del cálculo numérico CFD para el estudio de actuaciones de una motocicleta de competición de los alumnos Álvaro Ramos Cenzano y Mauro Botella Mompó. Se centra en el estudio de las fuerzas aerodinámicas ejercidas sobre una motocicleta de competición, en concreto de una Moto2 del campeonato de España de velocidad (CEV), por medio de programas de simulación. Los capítulos del 1 al 4, describen por qué surgió el Proyecto, los datos a tener en cuenta del reglamento oficial de Moto2 del CEV (centrándonos sobre todo en los aspectos que afectan a la Aerodinámica, como puede ser el carenado), y los objetivos que se pretenden hallar con este proyecto. Se hará una pequeña introducción a la aerodinámica general para después profundizar en la aerodinámica automovilística y sobretodo en la de las motocicletas. Pasando por el interés que tiene la aerodinámica a diferentes niveles (comercial y de competición), y su evolución a lo largo de la historia. Se analizarán los diferentes métodos de trabajo que se podrían usar para el estudio aerodinámico, centrándose sobre todo en la técnica de estudio informático por Dinámica de Fluidos Computacional o CFD. Dado que el proyecto está basado en unas motocicletas reales, surgirán una serie de problemas en el uso de los métodos numéricos que se irán solventando, intentando mantener la estructura lo más fiel posible a los modelos reales. Todos estos problemas y sus soluciones se detallarán con la intención de que puedan servir de ayuda para futuros estudios. Tras las simulaciones con los programas CFD correspondientes a los distintos modelos de motocicleta, se compararán los resultados para ayudar en la definición del modelo final. El proyecto concluirá con las conclusiones obtenidas por los alumnos.