Determinantes genéticos, bioquímicos e clínicos na resposta ao uso de hidroxiureia na doença falciforme

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Condições bacteriológicas da superfície das poltronas hematológicas par hemodiálise

Pós-graduação em Enfermagem (mestrado profissional) - FMB

Substituição parcial do farelo de soja pela farinha de carne e ossos em dietas para juvenis de tilápia-do-nilo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Padronização e implementação da técnica de pesquisa de mutação JAK2V617F no Laboratório de Biologia Molecular do Hemocentro de Botucatu e correlação da presença do transcrito bcr-abl nos pacientes com doença mieloproliferativa crônica

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Efeito da fragmentação florestal sobre parâmetros hematológicos em três espécies de morcegos (Artibeus lituratus, Carollia perspicillata e Sturnira lilium)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morbidades e consumo de álcool em pacientes internados na Clínica Médica de um hospital universitário

Abusive consumption of alcohol leads to several negative consequences to health and quality of life, as it increases the frequency of diseases that cause death or functional disabilities. The rates of patients admitted to hospital due to physical problems stemming from alcohol abuse are high. This study aimed at identifying patients according to variables age, gender and education level as well as at evaluating the prevalence of CAGE-positive patients and morbidity due to CID-10 (International Classification of Diseases) by the World Health Organization. It is a cross-sectional, observational, exploratory, descriptive and quantitative study. It was conducted at the Botucatu School of Medicine University Hospital (HC) from July to September 2010, at the Internal Medicine ward. This ward aggregates 5 specialties, namely, Cardiology, Hematology, General Medicine, Nephrology and Gastroclinic. Presently, it has 36 beds, of which four are for Intensive Care Therapy (ICT). Three hundred and ten medical charts were analyzed for identification of morbidities and of variables age, gender and schooling. A closed semistructured questionnaire including, among other questions, the CAGE test was applied to each individual. The CAGE test is a questionnaire that comprises four questions and considers that one who answers two or more of such questions affirmatively is a suspected case of alcoholism. The total population studied included 310 patients. Indexes of 60% (186) male and of 40% (124) female patients were found. As regards age range, a population with a higher prevalence of individuals from 51 to 70 years old (41%) was observed. The main education indexes were: incomplete Elementary School: 23.22% (72), complete Elementary School: 21.61% (67), incomplete Secondary School: 18.40% (57) and Illiteracy: 17.74% (55). As regards diseases, a higher proportion was observed for: ...(Complete abstract click electronic access below)

Valores hematológicos em Geochelone carbonária (Jabuti)

Hematology has a great importance in assessing the health of all animals, including reptiles, since it aids in the diagnosis of certain diseases and help to monitor patient's clinical evolution, although studies and articles in this area are rare, even with the increasing number of calls of these animals in veterinary clinics. Moreover, there are a lot of environmental and individual factors, which influence the hematological values of this group, such as age, sex, stress levels, nutrient levels and season which the exam was made, among others. For these reasons studies that aimed to evaluate the reference values of some specie, are essential to increase the knowledge in the field and to assist on the clinical approach. This paper aim’s to study the patterns of Hematology of Tortoise (Geochelone carbonaria) seeking the greater knowledge of clinical pathology of this species and increasing the possibilities for diagnosis, monitoring clinical outcome, and assessment of the general state and treatment

Síndromes paraneoplásicas hematológicas em cães

Tumoral masses can cause several direct problems in the organism, such as invading organs and altering their functions, leading to other problems such as the Paraneoplastic syndrome. The paraneoplastic syndrome is an alteration in the structure and function of the body due to the non-invasive actions of the tumor, for example, liberating hormones, peptides, cytokines and leading to cross reactions between normal tissues. The syndrome can affect different locations in the body, being that some are indicative of specific tumors, however the interpretation of the clinical and pathological findings referring to this syndrome should be utilized in the diagnostic and treatment. In the hematological paraneoplastic syndrome, there are alterations that occur due to indirect actions of the tumor on the blood cellular elements and the coagulation system, and that are generally detected in routine clinical and laboratorial exams. This study objective is to aboard some hematological paraneoplastic syndromes in dogs such as: anemia, thrombocytopenia, neutrophilic leukocytosis, hypergammaglobulinemia and erythrocytosis, emphasizing the different etiologies that may assist in differential diagnosis and the principal neoplasm related to this syndrome. Besides this, the early discovery and treatment of the paraneoplatic syndrome is important as the tumor itself, as it improves the prognostic and quality of life of the patient

Alterações hematológicas associadas ao hifema em cães

Hyphema is an intraocular hemorrhage that can result from many ocular and/or systemic disorders. It is an important clinical sign, since it may appear in the initial stages of some diseases or haematological disorders, acting as an initial marker for early diagnosis. The hematologic disturbances that predispose animals to present hyphema are: Thrombocytopenia, von Willebrand Disease, Scott Syndrome, Disseminated Intravascular Coagulation and Hyperviscosity Syndrome due to Multiple Myeloma. Each of the differential diagnoses due to hematological changes from the appearance of hyphema in dogs shows clinical relevance and particular ocular signs

Mananoligossacarídeo na alimentação de tilápia-do-Nilo (Oreochromis niloticus): avaliação de indicadores imunológicos, hematológicos, bioquímicos e de desempenho produtivo. Resposta ao desafio com Lactococcus garvieae

Pós-graduação em Microbiologia Agropecuária - FCAV

Evaluation of zebu nellore cattle blood samples using the Cell-Dyn 3500 hematology analyzer

The Cell-dyn 3500 is a multiparameter flow cytometer, which may analyze samples from several species performing several simultaneous analyses. It is able to perform white blood cells, red blood cells and platelet counts, besides differential leukocyte counts, packed cell volume and hemoglobin determination. Cell-Dyn 3500 performs total leukocyte count both optically and by impedance. The equipment may choose one or other method, based on the reliability of the results. Erythrocyte and platelet counts are determined by impedance. Leukocyte differentiation is based on an optical principle, using separation in multiangular polarized light. The objective of this study was to compare the results of complete blood count of Zebu Nellore heifers from Cell-dyn 3500, with those obtained from a semi-automated cell counter (Celm CC 510) and the manual technique. Blood samples were collected from the jugular vein in 5 mL EDTA vacuum tubes from 58 Nellore heifers, at 24 months of age. Samples were processed in parallel in the three different techniques. Results were analyzed using paired t test, Pearson's correlation and the Bland-Altmann method. There was a strong correlation for all parameters analyzed by Cell-Dyn 3500, manual method and semi-automated cell counter, except for basophils and monocytes counts. These results confirm that this analyzer is reliable for blood samples analysis of zebu cattle.

Perfil hematológico de tilápias-do-nilo alimentadas com dietas contendo diferentes lipídeos e estimuladas por baixa temperatura

The goal of this paper was to determine the effects of seven diets with differents fatty acids sources in hematology of Nile tilapia before and after cold stimulus. Seven diets, with 3% of fatty acid source, were formulated: soybean oil (OS); fish oil (OP); beef tallow (SB) and mixtures of OS+OP; OS+SB; OP+SB e OS+OP+SB. Fish (112 male of Nile tilapia of 30,0 +/- 2,65g) were totally randomly distributed, with four replicates, into 28 plastic aquaria (40 L; 4 fish per aquarium). Fish were fed four times per day for 35 days. At 35th day and also after seven days cold stimulus blood was collected and the profile hematological was evaluated. Leucocytes numbers were reduced in fish fed a SB and fish oil combination diet and were highest in fish fed SB diet before cold stimulus. Plasma protein was reduced in fish fed soybean oil diets and highest in fishes fed a combination of soybean oil, fish oil and SB diets. Anything fatty acids source promotes effective changes in fish health and cold tolerance promotion. Cold don't drastically reduced fish general health status.

Bullfrog hemogram under management stress

Stress is one of the major obstacles in frog culture and can be caused by factors such as inappropriate farming systems; inadequate management among other situations. The objective of the present study was to assess the hemogram, erythrogram and leukogram of bullfrogs (L. catesbeianus) when exposed to stress caused by different types of management: density and handling (manipulation), developed in the laboratory and repeated in the field for the appropriate comparisons in a experimental period of 30 days. The density experiment was conducted with four treatments: 70 animals m(-2) (D70); 100 animals m(-2) (D100), Control; 150 animals m(-2) (D150) and 200 animals m(-2) (D200), with 10, 14, 21 and 28 animals/box in the laboratory, respectively. Each treatment was performed with three simultaneous replicates. The handling experiment was conducted with three treatments: Treatment Without Handling (WH); Treatment with Partial Handling (PH) every 15 days and Treatment with Total Handling (TH) every 15 days. Each treatment was performed with four simultaneous replications. The methodology of the blood analysis followed international recommendations. In the present study we could observe that the animals of the field experiment did not reflect the same stress response observed in the laboratory in both experiment, which demonstrated the plasticity of these animals.

Analysis of the feasibility of early hospital discharge after autologous hematopoietic stem cell transplantation and the implications to nursing care

Autologous hematopoietic stem cell transplantation is a conduct used to treat some hematologic diseases and to consolidate the treatment of others. In the field of nursing, the few published scientific studies on nursing care and early hospital discharge of transplant patients are deficient. Knowledge about the diseases treated using hematopoietic stem cell transplantation, providing guidance to patients and caregivers and patient monitoring are important nursing activities in this process. Guidance may contribute to long-term goals through patients' short-term needs. To analyze the results of early hospital discharge on the treatment of patients submitted to autologous transplantation and the influence of nursing care on this conduct. A retrospective, quantitative, descriptive and transversal study was conducted. The hospital records of 112 consecutive patients submitted to autologous transplantation in the period from January to December 2009 were revisited. Of these, 12 patients, who remained in hospital for more than ten days after transplantation, were excluded from the study. The medical records of 100 patients with a median age of 48.5 years (19-69 years) were analyzed. All patients were mobilized and hematopoietic stem cells were collected by leukapheresis. The most common conditioning regimes were BU12Mel100 and BEAM 400. Toxicity during conditioning was easily managed in the outpatient clinic. Gastrointestinal toxicity, mostly Grades I and II, was seen in 69% of the patients, 62% of patients had diarrhea, 61% of the patients had nausea and vomiting and 58% had Grade I and II mucositis. Ten patients required hospitalization due to the conditioning regimen. Febrile neutropenia was seen in 58% of patients. Two patients died before Day +60 due to infections, one with aplasia. The median times to granulocyte and platelet engraftment were 12 days and 15 days, respectively, with median red blood cell and platelet transfusions until discharge of three and four units, respectively. Twenty-three patients required rehospitalization before being discharged from the outpatient clinic. The median time to granulocyte engraftment was 12 days and during the aplasia phase few patients were hospitalized or suffered infections. The toxicity of the conditioning was the leading cause of rehospitalization. The nursing staff participated by providing guidance to patients and during the mobilization, transplant and outpatient follow-up phases, thus helping to successfully manage toxicity.