Pseudoknots in prion protein mRNAs confirmed by comparative sequence analysis and pattern searching

The human prion gene contains five copies of a 24 nt repeat that is highly conserved among species. An analysis of folding free energies of the human prion mRNA, in particular in the repeat region, suggested biased codon selection and the presence of RNA patterns. In particular, pseudoknots, similar to the one predicted by Wills in the human prion mRNA, were identified in the repeat region of all available prion mRNAs available in GenBank, but not those of birds and the red slider turtle. An alignment of these mRNAs, which share low sequence homology, shows several co-variations that maintain the pseudoknot pattern. The presence of pseudoknots in yeast Sup35p and Rnq1 suggests acquisition in the prokaryotic era. Computer generated three-dimensional structures of the human prion pseudoknot highlight protein and RNA interaction domains, which suggest a possible effect in prion protein translation. The role of pseudoknots in prion diseases is discussed as individuals with extra copies of the 24 nt repeat develop the familial form of Creutzfeldt–Jakob disease.

Analysis of the Brominated Dioxin and Furan Emission Congener Pattern from Different Sources

Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F) have been studied for several decades and are well-known as unintentionally generated persistent organic pollutants (POPs), which pose serious health and environmental risks on a global scale1. Polybrominated dibenzo-p-dioxins and dibenzofurans (PBDD/F) have similar properties and effects to PCDD/F, as they are structural analogs with all the chlorine atoms substituted by bromine atoms. PBDD/F have been found in various matrices such as air, sediments, marine products, and human adipose samples.

Analysis of the future effects of the fuel shortage and increased small car usage upon traffic deaths and injuries. Final report. Executive summary.

Transportation Department, Office of Systems Engineering, Washington, D.C.

Tourist traffic in small historic cities: analysis, strategies, and recommendations. Final report.

Transportation Department, Office of University Research, Washington, D.C.

State of Rhode Island Special Adjudication for Enforcement - volume III: analysis of the administrative adjudication of traffic offenses. Analytic study. Final report.

National Highway Traffic Safety Administration, Office of Driver and Pedestrian Programs, Washington, D.C.

A comparable systems analysis: comparing automated highway systems to air traffic management. Final report.

Federal Highway Administration, Washington, D.C.

Community traffic safety programs: review and analysis.

Mode of access: Internet.

An analysis of some traffic problems on the campus of the University of California, Santa Barbara.

Mode of access: Internet.

An analysis of the impact of ASAP on the traffic safety system.

National Highway Traffic Safety Administration, Washington, D.C.

Movable concrete median barrier: risk analysis of deflection into opposing traffic.

Federal Highway Administration, Office of Research, Development, and Technology, Washington, D.C.

Analysis and forecast of truck traffic loads and the relative damage to pavement systems as a function of axle configurations. Final report.

Texas State Department of Highways and Public Transportation, Transportation Planning Division, Austin

Spectral analysis of the effects of daylight saving time on motor vehicle fatal traffic accidents. Final report.

National Highway Traffic Safety Administration, Mathematical Analysis Division, Washington, D.C.

An analysis of the impact of ASAP on the traffic safety system.

National Highway Traffic Safety Administration, Washington, D.C.

Analysis of the adjudication of alcohol related traffic arrests. Final report.

National Highway Traffic Safety Administration, Office of Driver and Pedestrian Programs, Washington, D.C.

An analysis of the traffic safety system. 1975. Final report.

National Highway Traffic Safety Administration, Washington, D.C.