998 resultados para Tyrol, Comté du


Relevância:

20.00% 20.00%

Publicador:

Resumo:

本论文由两部分组成,一、构建来自小麦的COMT的反义表达载体,转化烟草,研究抑制内源COMT对植物木质素合成及其生长发育的影响;二、利用花粉管通道法,将正义和反义COMT基因转化小麦,获得转基因小麦,从而进一步分析。 一、 反义抑制COMT对植物木质素合成及其生长发育的影响 构建含有小麦的咖啡酸-O-甲基转移酶(COMT)cDNA的反义表达载体, 利用农杆菌法转化烟草。 PCR, PCR-Southern 检测显示目的基因片段成功转入烟草基因组。处于营养生长期的转基因植株表型与对照没有明显差异;而发育成熟的转基因植株的植株矮化,茎部木质素含量与对照差异不大,木质素的组成S/G比下降,部分木质部细胞发生变形。我们还发现转基因烟草种子发芽率提高,移栽2个月的子一代转基因植株光合速率、蒸腾速率有所增强。结果表明通过反义抑制COMT将影响木质素合成,并在不同的发育阶段,影响着植物的生长发育。 二、 利用花粉管通道法获得转基因小麦 将构建好的含有Bar基因的正义和反义COMT表达载体利用花粉管法转化两个小麦品种(H4564和C6001),共获得转基因处理的种子1117颗,重新播种后,发育成苗分别为321株,总成苗率为28.7%。通过除草剂PPT筛选,分别获得PPT抗性植株31株。PCR检测抗性植株,获得PCR检测阳性植株5株,总阳性率为0.45%。阳性植株分别为H4564反义处理株1株,C6001的正义和反义处理株各2株。对小麦的植株高度,分蘖数等生理性状的分析发现,转基因小麦的分蘖数减少,植株高度降低。这些生理性状的改变与COMT基因转化的关系将有待于进一步验证。

Relevância:

20.00% 20.00%

Publicador:

Resumo:

A light fishery for "Ndagala" (Strolothrissa tanganicae) has been practised for many years on Lake Tanganyika. Initially this had a low catch rate, but has since been developed by the introduction of an artisanal fishery unit based on the catamaran. A unit consists of a pair of metal canoes joined together. The fish are attracted by three lights mounted on the structure, and are caught with a pyramid-shaped lift net. Selected beaches have been reserved for the artisanal fishery and the numher of units operating has increased from 12 in 1957 to 538 in 1972. The mean annual catch per unit is 11,000 kg, which is not sufficient for the fishery to be economic. However, prediction of a possible mean catch as high as 40 tons year encouraged the Burundi Government to launch a project with help from the Freedom from Hunger Campaign. This was designed to develop the fishery by the creation of artisanal fishing centres, and to make available a large number of fully equipped catamarans which could be paid for by a system of hire-purchase. The success of the project has illustrated that the furnishing of adequate equipment can bring about a transformation of the traditional fishery.

Relevância:

20.00% 20.00%

Publicador:

Resumo:

2013

Relevância:

20.00% 20.00%

Publicador:

Resumo:

Trotter, David, Albucasis, Traitier de Cyrurgie: Edition de la traduction en ancien fran?ais de la Chirurgie d'Abu'l Qasim Halaf Ibn ?Abbas al-Zahrawi du manuscrit BNF, fran?ais 1318 (Niemeyer, 2005)

Relevância:

20.00% 20.00%

Publicador:

Resumo:

Trotter, David, 'Les manuscrits latins de la Chirurgia d'Albucasis et la lexicographie du latin m?di?val', Archivum Latinitatis Medii Aevi (Bulletin Du Cange) (2001) 59(1) pp.181-202 RAE2008

Relevância:

20.00% 20.00%

Publicador:

Resumo:

Première edition revue, corrigée, et précédée d'une Préface à la Mosaïque, dans le plus nouveau goût.

Relevância:

20.00% 20.00%

Publicador:

Resumo:

Dissertação de Mestrado apresentada à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Psicopedagogia Perceptiva.

Relevância:

20.00% 20.00%

Publicador:

Resumo:

info:eu-repo/semantics/published