Orp1, a member of the Cdc18/Cdc6 family of S-phase regulators, is homologous to a component of the origin recognition complex.

cdc18+ of Schizosaccharomyces pombe is a periodically expressed gene that is required for entry into S phase and for the coordination of S phase with mitosis. cdc18+ is related to the Saccharomyces cerevisiae gene CDC6, which has also been implicated in the control of DNA replication. We have identified a new Sch. pombe gene, orp1+, that encodes an 80-kDa protein with amino acid sequence motifs conserved in the Cdc18 and Cdc6 proteins. Genetic analysis indicates that orp1+ is essential for viability. Germinating spores lacking the orp1+ gene are capable of undergoing one or more rounds of DNA replication but fail to progress further, arresting as long cells with a variety of deranged nuclear structures. Unlike cdc18+, orp1+ is expressed constitutively during the cell cycle. cdc18+, CDC6, and orp1+ belong to a family of related genes that also includes the gene ORC1, which encodes a subunit of the origin recognition complex (ORC) of S. cerevisiae. The products of this gene family share a 250-amino acid domain that is highly conserved in evolution and contains several characteristic motifs, including a consensus purine nucleotide-binding motif. Among the members of this gene family, orp1+ is most closely related to S. cerevisiae ORC1. Thus, the protein encoded by orp1+ may represent a component of an Sch. pombe ORC. The orp1+ gene is also closely related to an uncharacterized putative human homologue. It is likely that the members of the cdc18/CDC6 family play key roles in the regulation of DNA replication during the cell cycle of diverse species from archaebacteria to man.

G1 phase arrest induced by Wilms tumor protein WT1 is abrogated by cyclin/CDK complexes.

WT1, the Wilms tumor-suppressor gene, maps to the human chromosomal region 11p13 and encodes a transcriptional repressor, WT1, implicated in controlling normal urogenital development. Microinjection of the WT1 cDNA into quiescent cells or cells in early to mid G1 phase blocked serum-induced cell cycle progression into S phase. The activity of WT1 varied significantly depending on the presence or absence of an alternatively spliced region located upstream of the zinc finger domain. The inhibitory activity of WT1 was abrogated by the overexpression of cyclin E/CDK2 as well as cyclin D1/CDK4. Furthermore, both CDK4- and CDK2-associated kinase activities were downregulated in cells overexpressing WT1, whereas the levels of CDK4, CDK2, and cyclin D1 expression were unchanged. These findings suggest that inhibition of the activity of cyclin/CDK complexes may be involved in mediating the WT1-induced cell cycle block.

Additional traffic assignment options for the TASSIM model. Phase I. Final report.

Transportation Department, Washington, D.C.

Application of active and passive suspension techniques to improve high speed ground vehicle performance - phase II. Final report.

Transportation Department, Office of University Research, Washington, D.C.

Test site instrumentation study - phase II. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Integrated traffic simulation model - phase I. Volume 4: revisions to the UTCS-1 traffic simulation model to enhance operational efficiency. Final report.

Federal Highway Administration, Washington, D.C.

Statewide pedestrian study. Phase I report: Maryland's overall pedestrian accident situation. Interim report.

Mode of access: Internet.

Pedestrian injury causation parameters. Phase II. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Integrated traffic simulation model, phase, I. Volume 1: executive summary. Final report.

Federal Highway Administration, Washington, D.C.

Integrated traffic simulation model - phase I. Volume 2: design of the TRAF system (TOS). Final report.

Federal Highway Administration, Washington, D.C.

Automatic audio signing - literature survey analysis. Interim report, phase 1, task A.

Federal Highway Administration, Washington, D.C.

Computer simulation of the effect of road roughness on tire-pavement forces for an articulated vehicle performing braking and cornering maneuvers. Phase report.

Federal Highway Administration, Washington, D.C.

Handling and disposal of sludges from combined sewer overflow treatment : phase 1, characterization /

Mode of access: Internet.

Handling and disposal of sludges from combined sewer overflow treatment : phase II, impact assessment /

Mode of access: Internet.

Field testing, application of combustion modifications to control pollutant emissions from industrial boilers : phase II /

Mode of access: Internet.