188 resultados para SELFISH OPERONS


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Este estudo teve como principal objetivo a análise dos fatores determinantes que levaram os bombeiros voluntários de uma corporação para a prática de voluntariado. Nesta investigação, começamos por fazer um enquadramento teórico sobre as perspetivas e tipos de motivação do voluntariado, focando-nos depois em alguns aspetos da sua prática em Portugal. A componente empírica deste estudo apoiou-se numa análise quantitativa a 92 questionários realizados a bombeiros voluntários, adaptados de um estudo realizado por Ward e Mckillop (2011) com uma escala do tipo Likert de 1 a 7 pontos. A análise da distribuição por sexo apontou uma proporção de homens superior à das mulheres (63 = 68,5% vs. 29 = 31,5%), com idades compreendidas entre os 17 e os 71 anos, sendo a média das mesmas de 37,77 anos (dp = ± 12,1). No que concerne ao estado civil, dos inquiridos (35 = 38%) são casados. Quanto à escolaridade, a moda estatística situou-se no secundário (52 = 56,5%). Os itens que compuseram a escala de motivação para o voluntariado apresentaram pontuações superiores a 4 pontos numa escala do tipo Likert de 7 pontos. A escala constituída por 17 itens relaciona os diferentes tipos de motivação, designadamente, social, interesse, prazer, material-egoísta, egoísta, altruísta, necessidade e dever moral. Estes itens foram avaliados nas seguintes variáveis: sexo, classe etária, escolaridade e antiguidade. As conclusões retiradas deste estudo revelaram, dominantemente, motivações do tipo altruísta e social, para a prática do voluntariado. Estes resultados basearam-se nos valores das significâncias estatísticas (p). Quanto à motivação dos elementos do sexo masculino e do sexo feminino, verificaram-se em ambas maiores percentagens no tipo de motivação altruísta. Na análise realizada consoante a classe etária, as respostas que obtiveram maior percentagem foram relativamente ao item altruísta. No que concerne à escolaridade, os inquiridos com ensino básico e secundário apresentaram um maior número de respostas nos itens altruísta e social. Nos licenciados o item com maior percentagem foi o dever moral. Na análise feita relativamente à interseção entre a motivação e os anos de voluntariado, obtiveram-se índices de motivação muito diferenciados, entre os quais interesse, prazer, social, egoísta, material-egoísta e necessidade. / This study aimed to the analysis of the determining factors that led the volunteer firefighters of a corporation to practice voluntary. In this investigation, we begin by making a theoretical framework on the perspectives and types of volunteer motivation, focusing us then in some aspects of their practice in Portugal. The empirical component of the study was supported on a quantitative analysis of 92 questionnaires by volunteer firefighters, adapted from a study by Ward & McKillop (2011) with a Likert scale from 1 to 7 points. The gender distribution, of the analysis indicated a ratio of greater than men to women (63 = 68.5% vs. 29 = 31.5%), aged between 17 and 71 years, and the average of 37 to 77 years (SD = ± 12.1). With regard to marital status (35 = 38%) are married. As for education, the statistical mode stood in the secondary on (52 = 56.5%). The items comprising the motivation scale for volunteering had scores greater than 4 points on a Likert scale of 7 points. The scale consists of 17 items lists the different types of motivation, namely social, interest, pleasure, material-selfish, selfish, selfless, necessity and moral duty. These items were evaluated in the following variables: gender, age group, education level and years of service. Conclusions from this study revealed mainly motivations of altruistic and social type for volunteering. These results were based on the values of statistical significance (p). Both male and female elements replied with the highest percentage for the type of altruistic motivation. In the analysis carried out according to age group, the answers with the greatest percentage were relative to the altruistic item. With regard to education respondents with elementary and high school education had a higher number of responses in altruistic and social items. At University graduate level the item with the highest percentage was the moral duty. In the analysis regarding the intersection between motivation and years of volunteering very different motivation indices were obtained including interest, pleasure, social, selfish, material-selfish and need.

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Le présent mémoire entend analyser la pratique de l’auto-filmage dans deux films : La Pudeur ou l’impudeur (Hervé Guibert) et Tarnation (Jonathan Caouette). Nous regroupons ces long-métrages sous l’étiquette « auto-filmage pathographique ». Le malade, s’émancipant de l’imagerie médicale et des pratiques cinématographiques institutionnelles, reprend l’image filmique à son compte, aidé en cela par une technologie toujours plus ergonomique. Cette résurgence de l’image du corps malade dans le champ social ne se fait pas sans heurt ; l’exposition de corps décharnés et agoniques convoque un imaginaire catastrophiste et contredit les rituels d’effacement du corps auxquels procède la société occidentale. La forme que prend le récit de soi dans l’auto-filmage pathographique dépend de la maladie qui affecte chaque créateur. Nous observons une redéfinition de la sincérité, en lien avec l’exercice autobiographique. Il s’agit d’utiliser, dans l’auto-filmage pathographique, certains procédés fictionnels pour créer un discours sur soi-même dont la véracité repose sur d’autres critères que ceux communément admis. L’auto-filmage pathographique suppose en ce sens un véritable changement d’attitude et la mise en place de techniques de soi. Il induit une forme de réconciliation avec sa propre identité physique et psychique. En cela, l’écriture filmique de soi est un agent transformateur de la vie et un exercice spirituel. Les réalisateurs ne sont cependant pas uniquement tournés vers eux-mêmes. Chacun inclut quelques privilégiés au coeur de sa démarche. Le soin de soi, dans l’auto-filmage pathographique, ne se désolidarise pas du soin des autres. Auto-filmage et caméra subjective entretiennent un lien dialectique qui donne son sens à l’auto-filmage pathographique et voit leur antagonisme éclater. L’individu s’auto-filmant n’est pas seul ; sa démarche n’est pas qu’un solipsisme. Elle se voit dépassée par l’émergence de l’autre dans le champ ou parfois même, sa prise en main de la caméra.

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Acinetobacter baumannii , a strictly aerobic, non-fermentative, Gram-negative coccobacillary rod-shaped bacterium, is an opportunistic pathogen in humans. We recently isolated a multidrug-resistant A. baumannii strain KBN10P02143 from the pus sample drawn from a surgical patient in South Korea. We report the complete genome of this strain, which consists of 4,139,396 bp (G + C content, 39.08%) with 3,868 protein-coding genes, 73 tRNAs and six rRNA operons. Identification of the genes related to multidrug resistance from this genome and the discovery of a novel conjugative plasmid will increase our understanding of the pathogenicity associated with this species.

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Dissertação de Mestrado, Biologia Molecular e Microbiana, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2016

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In recent years, there has been an enormous growth of location-aware devices, such as GPS embedded cell phones, mobile sensors and radio-frequency identification tags. The age of combining sensing, processing and communication in one device, gives rise to a vast number of applications leading to endless possibilities and a realization of mobile Wireless Sensor Network (mWSN) applications. As computing, sensing and communication become more ubiquitous, trajectory privacy becomes a critical piece of information and an important factor for commercial success. While on the move, sensor nodes continuously transmit data streams of sensed values and spatiotemporal information, known as ``trajectory information". If adversaries can intercept this information, they can monitor the trajectory path and capture the location of the source node. This research stems from the recognition that the wide applicability of mWSNs will remain elusive unless a trajectory privacy preservation mechanism is developed. The outcome seeks to lay a firm foundation in the field of trajectory privacy preservation in mWSNs against external and internal trajectory privacy attacks. First, to prevent external attacks, we particularly investigated a context-based trajectory privacy-aware routing protocol to prevent the eavesdropping attack. Traditional shortest-path oriented routing algorithms give adversaries the possibility to locate the target node in a certain area. We designed the novel privacy-aware routing phase and utilized the trajectory dissimilarity between mobile nodes to mislead adversaries about the location where the message started its journey. Second, to detect internal attacks, we developed a software-based attestation solution to detect compromised nodes. We created the dynamic attestation node chain among neighboring nodes to examine the memory checksum of suspicious nodes. The computation time for memory traversal had been improved compared to the previous work. Finally, we revisited the trust issue in trajectory privacy preservation mechanism designs. We used Bayesian game theory to model and analyze cooperative, selfish and malicious nodes' behaviors in trajectory privacy preservation activities.

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The relative role of drift versus selection underlying the evolution of bacterial species within the gut microbiota remains poorly understood. The large sizes of bacterial populations in this environment suggest that even adaptive mutations with weak effects, thought to be the most frequently occurring, could substantially contribute to a rapid pace of evolutionary change in the gut. We followed the emergence of intra-species diversity in a commensal Escherichia coli strain that previously acquired an adaptive mutation with strong effect during one week of colonization of the mouse gut. Following this first step, which consisted of inactivating a metabolic operon, one third of the subsequent adaptive mutations were found to have a selective effect as high as the first. Nevertheless, the order of the adaptive steps was strongly affected by a mutational hotspot with an exceptionally high mutation rate of 10-5. The pattern of polymorphism emerging in the populations evolving within different hosts was characterized by periodic selection, which reduced diversity, but also frequency-dependent selection, actively maintaining genetic diversity. Furthermore, the continuous emergence of similar phenotypes due to distinct mutations, known as clonal interference, was pervasive. Evolutionary change within the gut is therefore highly repeatable within and across hosts, with adaptive mutations of selection coefficients as strong as 12% accumulating without strong constraints on genetic background. In vivo competitive assays showed that one of the second steps (focA) exhibited positive epistasis with the first, while another (dcuB) exhibited negative epistasis. The data shows that strong effect adaptive mutations continuously recur in gut commensal bacterial species.

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This essay is an ecocritical literary analysis of the novel Solar. The aim is to investigate the effect on the reader of the main characters, place, symbols and satire with focus on climate change and sustainability. The protagonist Michael Beard, a despicable, selfish antihero, consumes everything and everybody whereas Tom Aldous, the heroic character, is devoted to a life of sustainability. Beard represents the greedy capitalistic Western consumer society and its male leaders who close their eyes to the warnings of a changing climate, and Aldous represents the sustainable and alternative lifestyle. Humor and ridicule exhibits the weaknesses and greed of mankind in a satirical way. Symbols like cancer and the Co2 emitting transport system symbolize overconsumption and a stressed globe. The effect on the reader is a reflection about the ongoing struggle between climate change and sustainability and the demand for a change towards a sustainable lifestyle.