Identification and characterization of pptA: a gene involved in the phase-variable expression of phosphorylcholine on pili of Neisseria meningitidis

Pili of pathogenic Neisseria are major virulence factors associated with adhesion, cytotoxicity, twitching motility, autoaggregation, and DNA transformation. Pili are modified posttranslationally by the addition of phosphorylcholine. However, no genes involved in either the biosynthesis or the transfer of phosphorylcholine in Neisseria meningitidis have been identified. In this study, we identified five candidate open reading frames (ORFs) potentially involved in the biosynthesis or transfer of phosphorylcholine to pilin in N. meningitidis. Insertional mutants were constructed for each ORF in N. meningitidis strain C311#3 to determine their effect on phosphorylcholine expression. The effect of the mutant ORFs on the modification by phosphorylcholine was analyzed by Western analysis with phosphorylcholine-specific monoclonal antibody TEPC-15. Analysis of the mutants showed that ORF NMB0415, now defined as pptA (pilin phosphorylcholine transferase A), is involved in the addition of phosphorylcholine to pilin in N. meningitidis. Additionally, the phase variation (high frequency on-off switching of expression) of phosphorylcholine on pilin is due to changes in a homopolymeric guanosine tract in pptA.

Validation of percutaneous puncture trajectory during renal access using 4D ultrasound reconstruction

Background: An accurate percutaneous puncture is essential for disintegration and removal of renal stones. Although this procedure has proven to be safe, some organs surrounding the renal target might be accidentally perforated. This work describes a new intraoperative framework where tracked surgical tools are superimposed within 4D ultrasound imaging for security assessment of the percutaneous puncture trajectory (PPT). Methods: A PPT is first generated from the skin puncture site towards an anatomical target, using the information retrieved by electromagnetic motion tracking sensors coupled to surgical tools. Then, 2D ultrasound images acquired with a tracked probe are used to reconstruct a 4D ultrasound around the PPT under GPU processing. Volume hole-filling was performed in different processing time intervals by a tri-linear interpolation method. At spaced time intervals, the volume of the anatomical structures was segmented to ascertain if any vital structure is in between PPT and might compromise the surgical success. To enhance the volume visualization of the reconstructed structures, different render transfer functions were used. Results: Real-time US volume reconstruction and rendering with more than 25 frames/s was only possible when rendering only three orthogonal slice views. When using the whole reconstructed volume one achieved 8-15 frames/s. 3 frames/s were reached when one introduce the segmentation and detection if some structure intersected the PPT. Conclusions: The proposed framework creates a virtual and intuitive platform that can be used to identify and validate a PPT to safely and accurately perform the puncture in percutaneous nephrolithotomy.

A política de cooperação portuguesa com os PALOP: contributos do Ensino Superior Politécnico

RESUMO: É longínqua e indiscutível a ligação que se estabelece entre Portugal e os Países Africanos de Língua Oficial Portuguesa. No entanto, a manutenção dos laços de entreajuda e cooperação, embora desejada e expressamente manifestada por ambas as partes, nem sempre se consubstancia em acções concretas. É neste contexto que se insere o presente trabalho, tendo como objectivo investigar de que modo se materializa a Política de Cooperação entre Portugal e os Países Africanos de Língua Oficial Portuguesa, ao nível do Ensino Superior. Avaliamos concretamente a actuação das Instituições de Ensino Superior Politécnico, junto dos alunos oriundos daqueles países, no domínio da Acção Social e de que modo pode existir uma acção diferenciadora entre Instituições, capaz de captar alunos e de os acolher em melhores condições. Perante as carências e fragilidades encontradas naquela população, concluímos que existe um vasto campo de actuação onde as Instituições de Ensino Superior podem realizar os propósitos enunciados nos Acordos de Cooperação, estabelecidos com aqueles Países. Actuando em prol destes alunos efectivam assim as resoluções enunciadas para a Política de Cooperação Portuguesa em matéria de educação e divulgação da língua e cultura portuguesas, contribuindo ainda, para o desenvolvimento daqueles países. ABSTRACT: It is far and indisputable the link established between Portugal and the African Countries of Portuguese Official Language (PALOP). However, the maintenance of ties of mutual aid and cooperation, though desired and explicitly expressed by both sides, has not always been embodied in concrete actions. It is this context that frames the present work, aiming to investigate how the policy of cooperation between Portugal and the African Countries of Portuguese Official Language is materialized at the Higher Education level. We specifically evaluate the performance of Polytechnic Higher Education Institutions with the students from those countries in the field of social aid and how a differentiator action between institutions can be in place, capable of capturing students and welcoming them in a better way. In face of the needs and weaknesses found in that population, we conclude that there is a vast field of activity where HEI can realize the goals set out in the Cooperation Agreements established with those countries. Acting on behalf of these students they put to practice the resolutions set out for the Portuguese Cooperation Policy in education and dissemination of the Portuguese language and culture, thus contributing to the development of those countries.

Dimensionamento de um silo em betão armado para stockagem de cimento com fundações indirectas

O presente trabalho é o corolário do curso de engenharia civil, para a obtenção do grau de Mestre, tendo-se optado pela elaboração do dimensionamento de um Silo em betão armado. O projecto inclui os elementos necessários para uma correcta execução desta estrutura. Os silos são construções, que em função da sua finalidade de utilização estão sujeitos a diversas acções peculiares. No presente trabalho foram consideradas as acções, indicadas nos Eurocódigos para este tipo de estruturas, nomeadamente a acção gerada pela presença do material ensilado, que conduz a um conjunto de pressões horizontais e verticais nas paredes do Silo, bem como a acção causada pelo gradiente de temperatura, devido ao material ensilado (120ºC). O estudo realizado para o dimensionamento do Silo, foi feito considerando a discretização do mesmo através de um programa de elementos finitos, tendo a introdução da estrutura sido realizada em computador com recurso ao “SAP2000”. Foram estudados 2 modelos: O primeiro partindo de uma configuração unifilar em que se consideraram apenas elementos ditos frames (barras), o qual serviu basicamente para o pré dimensionamento da estrutura. O segundo mais completo em que a estrutura foi analisada a partir de um conjunto que incluía a modelação de todos os elementos previamente dimensionados, com recurso a outros softwares (Robot), folhas de cálculo, tabelas e ábacos. Reuniram-se também elementos relativos a estruturas deste tipo tendo-se consultado bibliografia da especialidade, de modo a adquirir um conhecimento mais profundo necessário à realização deste projecto.

Compression Efficiency Analysis of Wyner-Ziv Video Coding with Motion Compensated Side Information Interpolation

The Wyner-Ziv video coding (WZVC) rate distortion performance is highly dependent on the quality of the side information, an estimation of the original frame, created at the decoder. This paper, characterizes the WZVC efficiency when motion compensated frame interpolation (MCFI) techniques are used to generate the side information, a difficult problem in WZVC especially because the decoder only has available some reference decoded frames. The proposed WZVC compression efficiency rate model relates the power spectral of the estimation error to the accuracy of the MCFI motion field. Then, some interesting conclusions may be derived related to the impact of the motion field smoothness and the correlation to the true motion trajectories on the compression performance.

A flexible side information generation framework for distributed video coding

One of the most efficient approaches to generate the side information (SI) in distributed video codecs is through motion compensated frame interpolation where the current frame is estimated based on past and future reference frames. However, this approach leads to significant spatial and temporal variations in the correlation noise between the source at the encoder and the SI at the decoder. In such scenario, it would be useful to design an architecture where the SI can be more robustly generated at the block level, avoiding the creation of SI frame regions with lower correlation, largely responsible for some coding efficiency losses. In this paper, a flexible framework to generate SI at the block level in two modes is presented: while the first mode corresponds to a motion compensated interpolation (MCI) technique, the second mode corresponds to a motion compensated quality enhancement (MCQE) technique where a low quality Intra block sent by the encoder is used to generate the SI by doing motion estimation with the help of the reference frames. The novel MCQE mode can be overall advantageous from the rate-distortion point of view, even if some rate has to be invested in the low quality Intra coding blocks, for blocks where the MCI produces SI with lower correlation. The overall solution is evaluated in terms of RD performance with improvements up to 2 dB, especially for high motion video sequences and long Group of Pictures (GOP) sizes.

Adaptive deblocking filter for transform domain Wyner-Ziv video coding

Wyner-Ziv (WZ) video coding is a particular case of distributed video coding, the recent video coding paradigm based on the Slepian-Wolf and Wyner-Ziv theorems that exploits the source correlation at the decoder and not at the encoder as in predictive video coding. Although many improvements have been done over the last years, the performance of the state-of-the-art WZ video codecs still did not reach the performance of state-of-the-art predictive video codecs, especially for high and complex motion video content. This is also true in terms of subjective image quality mainly because of a considerable amount of blocking artefacts present in the decoded WZ video frames. This paper proposes an adaptive deblocking filter to improve both the subjective and objective qualities of the WZ frames in a transform domain WZ video codec. The proposed filter is an adaptation of the advanced deblocking filter defined in the H.264/AVC (advanced video coding) standard to a WZ video codec. The results obtained confirm the subjective quality improvement and objective quality gains that can go up to 0.63 dB in the overall for sequences with high motion content when large group of pictures are used.

Reabilitação térmica e energética de edifício antigo: aplicação ao antigo edifício dos CTT em Coruche

O estudo desenvolvido no presente documento teve como alvo um edifício antigo construído entre 1930 e 1940 no concelho de Coruche, com construção característica da sua época. Numa fase inicial analisou-se de que forma se comporta o edifício em termos térmicos e energéticos, com base nas metodologias de cálculo apresentadas pelo regulamento térmico em vigor (RCCTE, 2006), e de seguida foram analisadas várias soluções existentes para a reabilitação térmica e energética do edifício que poderá ser concretizada, essencialmente devido às soluções para isolamento térmico das paredes, pavimentos e cobertura, assim como devido às soluções para vidros e caixilharia dos vãos envidraçados. Numa fase posterior, foi estudada a aplicação isolada das várias soluções de reabilitação consideradas, tendo sido simulada a aplicação de várias espessuras de isolante para as soluções da envolvente opaca e vários tipos de vidro duplo e caixilharia para as soluções da envolvente envidraçada, de forma a perceber-se a implicação que cada uma tem quanto à variação das necessidades energéticas do edifício. Por fim, foram calculados os custos de aplicação das várias soluções estudadas, com auxílio de um software de orçamentação, de forma que estas possam ser comparadas em termos da relação custo/benefício, em que, o benefício de cada solução será a variação provocada nas necessidades energéticas do edifício com aplicação da mesma. Assim, procurará encontrar-se para o edifício em estudo não só as soluções mais vantajosas em termos energéticos mas também as mais rentáveis, e procurará perceber-se se um investimento numa intervenção de reabilitação deste género conduzirá a um período de retorno do investimento reduzido, que possa ser considerado aceitável.

Advanced side information creation techniques and framework for Wyner-Ziv video coding

Recently, several distributed video coding (DVC) solutions based on the distributed source coding (DSC) paradigm have appeared in the literature. Wyner-Ziv (WZ) video coding, a particular case of DVC where side information is made available at the decoder, enable to achieve a flexible distribution of the computational complexity between the encoder and decoder, promising to fulfill novel requirements from applications such as video surveillance, sensor networks and mobile camera phones. The quality of the side information at the decoder has a critical role in determining the WZ video coding rate-distortion (RD) performance, notably to raise it to a level as close as possible to the RD performance of standard predictive video coding schemes. Towards this target, efficient motion search algorithms for powerful frame interpolation are much needed at the decoder. In this paper, the RD performance of a Wyner-Ziv video codec is improved by using novel, advanced motion compensated frame interpolation techniques to generate the side information. The development of these type of side information estimators is a difficult problem in WZ video coding, especially because the decoder only has available some reference, decoded frames. Based on the regularization of the motion field, novel side information creation techniques are proposed in this paper along with a new frame interpolation framework able to generate higher quality side information at the decoder. To illustrate the RD performance improvements, this novel side information creation framework has been integrated in a transform domain turbo coding based Wyner-Ziv video codec. Experimental results show that the novel side information creation solution leads to better RD performance than available state-of-the-art side information estimators, with improvements up to 2 dB: moreover, it allows outperforming H.264/AVC Intra by up to 3 dB with a lower encoding complexity.

Short-term Feature Space and Music Genre Classification

In music genre classification, most approaches rely on statistical characteristics of low-level features computed on short audio frames. In these methods, it is implicitly considered that frames carry equally relevant information loads and that either individual frames, or distributions thereof, somehow capture the specificities of each genre. In this paper we study the representation space defined by short-term audio features with respect to class boundaries, and compare different processing techniques to partition this space. These partitions are evaluated in terms of accuracy on two genre classification tasks, with several types of classifiers. Experiments show that a randomized and unsupervised partition of the space, used in conjunction with a Markov Model classifier lead to accuracies comparable to the state of the art. We also show that unsupervised partitions of the space tend to create less hubs.

O programa de formação dos mentores: conceção e planeamento

Este texto define e enquadra teoricamente as principais finalidades e orientações metodológicas do programa de formação dos mentores que acompanharam os professores em período probatório. O referencial de formação dos mentores construiu-se em torno das diferentes dimensões do ato de ensinar. A metodologia de formação organizou-se a partir do conceito de desenvolvimento profissional, considerando o mentor como objeto e sujeito da formação e procurando articular o processo formativo e o trabalho de supervisão desenvolvido pelos mentores, no terreno. As sessões de formação foram planeadas estrategicamente a partir de questões orientadoras às quais se procurou dar resposta através de um conjunto de atividades e materiais. Durante a implementação do período probatório foi produzido pelos intervenientes um conjunto considerável de documentos que foram trabalhados durante a formação. - Abstract This article defines and frames theoretically the main purposes and the methodological guidelines of the training program of mentors who have accompanied teachers on the probationary period. Those guidelines were constructed around the different dimensions of the act of teaching. The training methodology was organized from the concept of professional development, considering the mentor as object and subject of training and seeking to articulate the formative process and supervision work developed by mentors on the field. Training sessions have been strategically planned from guiding questions which sought to respond through a range of activities and materials. During the implementation of the probationary period, the participants delivered a considerable set of documents, which were worked on during training.- Resumen Este texto define y enfoca teóricamente las principales finalidades y orientaciones metodológicas del programa de formación de los mentores que acompañaron a los profesores en el periodo probatorio. La referencia formativa de los mentores se construyó en torno de las distintas dimensiones del hecho de enseñar. La metodología formativa se organizó a partir del concepto de desarrollo profesional, considerando al mentor como objeto y sujeto de la formación y buscando articular el proceso formativo y el trabajo de supervisión desarrollado por los mentores, en el terreno. Las sesiones de formación se proyectaron estratégicamente a partir de planteos orientadores, a los cuales se les dio respuesta a través de un conjunto de actividades y materiales. Durante la implementación del periodo probatorio, los participantes produjeron un considerable conjunto de documentos que se elaboraron durante la formación.

Videovigilância, compressão e criptografia

Mestrado em Engenharia Electrotécnica e de Computadores

Improved matching criterion for frame rate upconversion with trilateral filtering

Frame rate upconversion (FRUC) is an important post-processing technique to enhance the visual quality of low frame rate video. A major, recent advance in this area is FRUC based on trilateral filtering which novelty mainly derives from the combination of an edge-based motion estimation block matching criterion with the trilateral filter. However, there is still room for improvement, notably towards reducing the size of the uncovered regions in the initial estimated frame, this means the estimated frame before trilateral filtering. In this context, proposed is an improved motion estimation block matching criterion where a combined luminance and edge error metric is weighted according to the motion vector components, notably to regularise the motion field. Experimental results confirm that significant improvements are achieved for the final interpolated frames, reaching PSNR gains up to 2.73 dB, on average, regarding recent alternative solutions, for video content with varied motion characteristics.

Processamento de imagem para determinação de distribuições granulométricas de gotas numa suspensão

Mestrado em Engenharia Electrotécnica e de Computadores

Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs.

A 17.6 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains twelve open reading frames (ORFs) longer than 100 amino acids. Three genes had already been cloned and sequenced: CCT, ADE3 and TR-I. Two ORFs are similar to other yeast genes: G7722 with the YAL023 (PMT2) and PMT1 genes, encoding two integral membrane proteins, and G7727 with the first half of the genes encoding elongation factors 1gamma, TEF3 and TEF4. Two other ORFs, G7742 and G7744, are most probably yeast orthologues of the human and Paracoccus denitrificans electron-transferring flavoproteins (beta chain) and of the Escherichia coli phosphoserine phosphohydrolase. The five remaining identified ORFs do not show detectable homology with other protein sequences deposited in data banks. The sequence has been deposited in the EMBL data library under Accession Number Z49133.