Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs.


Autoria(s): Guerreiro, Paulo; Barreiros, Tânia; Soares, Helena; Cyrne, Luísa; Maia e Silva, Alexandra; Rodrigues-Pousada, Claudina
Data(s)

15/01/2014

15/01/2014

01/03/1996

Resumo

A 17.6 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains twelve open reading frames (ORFs) longer than 100 amino acids. Three genes had already been cloned and sequenced: CCT, ADE3 and TR-I. Two ORFs are similar to other yeast genes: G7722 with the YAL023 (PMT2) and PMT1 genes, encoding two integral membrane proteins, and G7727 with the first half of the genes encoding elongation factors 1gamma, TEF3 and TEF4. Two other ORFs, G7742 and G7744, are most probably yeast orthologues of the human and Paracoccus denitrificans electron-transferring flavoproteins (beta chain) and of the Escherichia coli phosphoserine phosphohydrolase. The five remaining identified ORFs do not show detectable homology with other protein sequences deposited in data banks. The sequence has been deposited in the EMBL data library under Accession Number Z49133.

Identificador

Guerreiro P, Barreiros T, Soares H, Cyrne L, Maia e Silva A, Rodrigues-Pousada C. Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs. Yeast. 1996;12(3):273-80.

1097-0061

http://hdl.handle.net/10400.21/3090

Idioma(s)

eng

Publicador

Wiley

Relação

http://onlinelibrary.wiley.com/doi/10.1002/%28SICI%291097-0061%2819960315%2912:3%3C273::AID-YEA898%3E3.0.CO;2-1/abstract

Direitos

openAccess

Palavras-Chave #Saccharomyces cerevisiae #Chromosome sequencing #Chromosome VII #CCT #ADE3 #TR-1 #PMT #EF19 #Amino acid sequence #Chromosomes, Fungal #Escherichia coli #Genes, Fungal #Molecular sequence data #Open reading frames #Phosphoric monoester hydrolases #Sequence homology, Nucleic acid
Tipo

article