乌鳢(Channa argus)干扰素刺激基因Viperin和ISG15及其启动子的克隆与特征分析

应用抑制差减杂交、末端快速扩增和基因步移技术,克隆并鉴定了乌鳢干扰素刺激基因Viperin和ISG15及其启动子序列.Viperin cDNA全长1474nt,包含1059nt的开放阅读框,编码352个氨基酸.除N末端70个氨基酸外,Viperin蛋白氨基酸序列在鱼类和哺乳类具有高度的保守性.ISG15 cDNA全长758nt,包含468nt的开放阅读框,编码155个氨基酸,含有两个泛素样(UBL)结构域,C末端具有保守的UB偶联结构(LRGG).Viperin和ISG15启动子区存在保守的干扰素刺激反应

Multi-objective optimisation of horizontal axis wind turbine structure and energy production using aerofoil and blade properties as design variables

The design of wind turbine blades is a true multi-objective engineering task. The aerodynamic effectiveness of the turbine needs to be balanced with the system loads introduced by the rotor. Moreover the problem is not dependent on a single geometric property, but besides other parameters on a combination of aerofoil family and various blade functions. The aim of this paper is therefore to present a tool which can help designers to get a deeper insight into the complexity of the design space and to find a blade design which is likely to have a low cost of energy. For the research we use a Computational Blade Optimisation and Load Deflation Tool (CoBOLDT) to investigate the three extreme point designs obtained from a multi-objective optimisation of turbine thrust, annual energy production as well as mass for a horizontal axis wind turbine blade. The optimisation algorithm utilised is based on Multi-Objective Tabu Search which constitutes the core of CoBOLDT. The methodology is capable to parametrise the spanning aerofoils with two-dimensional Free Form Deformation and blade functions with two tangentially connected cubic splines. After geometry generation we use a panel code to create aerofoil polars and a stationary Blade Element Momentum code to evaluate turbine performance. Finally, the obtained loads are fed into a structural layout module to estimate the mass and stiffness of the current blade by means of a fully stressed design. For the presented test case we chose post optimisation analysis with parallel coordinates to reveal geometrical features of the extreme point designs and to select a compromise design from the Pareto set. The research revealed that a blade with a feasible laminate layout can be obtained, that can increase the energy capture and lower steady state systems loads. The reduced aerofoil camber and an increased L/. D-ratio could be identified as the main drivers. This statement could not be made with other tools of the research community before. © 2013 Elsevier Ltd.

Dynamics of photo-enhanced magneto-crystalline anisotropy in diluted ferromagnetic GaMnAs

By chopping a pump beam in conventional time-resolved Kerr rotation (TRKR) experiments and measuring the time evolution of M-shaped "major" hysteresis loops of magnetic linear dichroism (Delta MLD = MLDpump-on MLDpump-off), the differential MLD signal in the presence and the absence of the pump beam, we studied the dynamics of photo-enhanced magneto-crystalline anisotropy, and found that its very long recovering time (much longer than 13 ns) might reflect the nature of the coherent coupling between photo-excited holes and localized spins in the d shell of manganese.

Realization of low threshold of InGaAs/InAlAs quantum cascade laser

By optimizing the molecule beam epitaxy growth condition, the quality of quantum cascade (QC) material has greatly been improved. The spectrum of double x-ray diffraction indicates that the interface between the constituent layers is very smooth, the lattice mismatch between the epilayer and the substrate is less than 0.1%, and the periodicity fluctuation of the active region is not more than 4.2%. The QC laser with the emission wavelength of about 5.1 mum is operated at the threshold of 0.73 kA/cm(2) at liquid nitrogen temperature with the repetition rate of 10kHz and at a duty cycle of 1%. Meanwhile, the performance of the laser can be improved with suitable post process techniques such as the metallic ohmic contact technology.

Studies of high DC current induced degradation in III-V nitride based heterojunctions

We report experiments on high de current stressing in commercial III-V nitride based heterojunction light-emitting diodes. Stressing currents ranging from 100 mA to 200 mA were used. Degradations in the device properties were investigated through detailed studies of the current-voltage (I-V) characteristics, electroluminescence, deep-level transient Fourier spectroscopy and flicker noise. Our experimental data demonstrated significant distortions in the I-V characteristics subsequent to electrical stressing. The room temperature electro-luminescence of the devices exhibited a 25% decrement in the peak emission intensity. Concentration of the deep-levels was examined by deep-level transient Fourier spectroscopy, which indicated an increase in the density of deep-traps from 2.7 x 10(13) cm(-3) to 4.2 x 10(13) cm(-3) at E-1 = E-C - 1.1 eV. The result is consistent with our study of 1/f noise, which exhibited up to three orders of magnitude increase in the voltage noise power spectra. These traps are typically located at energy levels beyond the range that can be characterized by conventional techniques including DLTS. The two experiments, therefore, provide a more complete picture of trap generation due to high dc current stressing.

签密方案在分布式协议中的应用

该文基于可验证秘密共享思想和对Zheng的签密方案的必要修改，首次构造了一种不需要可信中心的门限签密方案．它能同时达到门限签名和加密的双重目的，实现代价仅和门限签名相当，并具有非否认性质．该文对当前一些分布式密钥分配协议做了分析，并基于Naor的基本思想，重点利用签密方案设计了协议SC—DKDS．与其它协议相比，该协议在减低实现成本等方面更为有效，因为它不需要认证信道、秘密信道及复杂的零知识证明等．该文还在RO（Random Oracle，随机预言）模型中给出了以上协议的安全性证明．

用于神经电信号记录的多通道微探针

以体硅为衬底，采用微机械加工技术(MEMS)制作了七通道的可植入到脑皮层的微探针，用于记录神经电信号．从生物相容性、减小植入损伤、工艺制作难度等方面考虑，制作了以二氧化硅／硅(SiO_2／Si)为主体的微探针，并详述了其具体制作工艺流程．扫描电镜(SEM)照片显示微探针针长3 mm、针宽100 μm、针厚约为20μm，各个记录点直径10μm、间距120μm，实现了各通道之间良好的信号隔离．微探针距离针尖1 mm范围内的针宽以一定弧度由100μm逐渐变窄，同时针尖锥角为6°，此种结构有利于减小植入时对脑组织的损伤．通过体外测试得到，当频率由10 kHz增加到10 MHz时，微电极各个通道阻抗由150.5 kΩ降低到6.0 kΩ．