O DISCURSO AMBIENTAL JORNALÍSTICO, POLÍTICO E EMPRESARIAL EM ÂMBITO LOCAL: uma proposta de análise

Esta tese tem como tema principal o discurso ambiental local. Trata-se de uma proposta de análise, que pode ser aplicada a este tipo de discurso em qualquer localidade, e um estudo de caso, que aplicou a análise proposta na tese. O objetivo geral desta pesquisa foi analisar a informação local sobre meio ambiente veiculada por meio dos discursos jornalístico, político e empresarial, estabelecendo relações históricas, sociais e ideológicas do discurso ambiental que permeiam as três áreas em questão. A metodologia incluiu três fases: pesquisa bibliográfica e revisão de literatura sobre os principais conceitos levantados no trabalho; levantamento de discursos locais disponíveis na mídia para análise no estudo de caso; análise dos discursos a partir de protocolo elaborado com base na Análise do Discurso de linha francesa. O estudo de caso traz a análise do discurso ambiental no município de Frutal-MG, onde hoje estão em andamento várias pesquisas e projetos na área ambiental. A principal conclusão do trabalho confirma a hipótese da pesquisa de que a informação ambiental fruto dos discursos político, jornalístico e empresarial em âmbito local tem caráter predominantemente situacional, mercadológico e propagandístico, pouco focada em conscientizar e educar e com ênfase em interesses comerciais e eleitorais e na resolução de problemas emergenciais

Children's TV and Digital Media in the Arab World

Who analyses children’s screen content and media use in Arab countries, and with what results? Children, defined internationally as under-18s, account for some 40 per cent of Arab populations and the proportion of under-fives is correspondingly large. Yet studies of children’s media and child audiences in the region are as scarce as truly popular locally produced media content aimed at children. At the very time when conflict and uncertainty in key Arab countries have made local development and diversification of children’s media more remote, it has become more urgent to gain a better understanding of how the next generation’s identities and world-views are formed. This interdisciplinary book is the first in English to probe both the state of Arab screen media for children and the practices of Arabic-speaking children in producing, as well as consuming, screen content. It responds to the gap in research by bringing together a holistic investigation of institutions and leading players, children’s media experiences and some iconic media texts. With children’s media increasingly linked to merchandising, which favours US-based global players and globalizing forces, this volume provides a timely insight into tensions between differing concepts of childhood and desirable media messages.

Enigmas monetarios en el derecho local. Los mencales y maravedís en los fueros castellanos

La historia de la moneda en la Castilla medieval ha estado siempre mediatizada por la convivencia no siempre armónica entre dos sistemas monetarios muy diferentes. Uno basado en la plata, de origen europeo, otro centrado en el oro, de raíces árabes. La necesidad de conectar y de establecer unas equivalencias entre ellos se convirtió pronto en una necesidad, máxime cuando las monedas de oro incrementaron sus variantes. En esta compleja situación aparecen mencales y maravedís citados conjuntamente en muchos fueros: Zorita, Uclés, Cuenca,... sin que su naturaleza quede del todo clara. Este artículo compara estas referencias y analiza las equivalencias que los unen. Como colofón al trabajo podemos afirmar que los maravedís citados en cada texto corresponden a monedas áureas de distinto peso y valor mientras el mencal es identificado con un ponderal de oro que los relaciona.

Renta feudal, ejercicio del poder y diferenciación social campesina en el marco del señorío del cabildo salmantino en la Edad Media

El presente trabajo analiza la evolución del señorío eclesiástico en el largo plazo, para sumar al conocimiento de las formas señoriales de la Extremadura leonesa. Consideramos específicamente el caso del cabildo catedralicio salmantino entre los siglos XII y XV. Buscamos demostrar que no poseyó idéntica estructura durante todo el período y que sus transformaciones se explican por una compleja conjunción de variables. Dichas transformaciones incidieron sobre las estructuras sociales del agro, en especial sobre el desarrollo de procesos de diferenciación social campesina. Demostramos que la forma concreta en que se realizaba la renta podía alterar las estructuras sociales de las comunidades y que el desarrollo de las relaciones sociales asalariadas se encontraba muy vinculado a las coyunturas económicas y a las posibilidades y límites de la gestión señorial. Finalmente, ponemos de relieve que la transformación social no siempre fue irreversible y que su consolidación dependió de la incapacidad de los señores de ejercer sus poderes políticos.

El impacto de la red social twitter en la información local del diario El Tiempo y El Mercurio en el periodo febrero-junio

La red social Twitter da sus comienzos en el año 2006, su idioma inicial fue el inglés, su principal función es la de crear textos de 140 caracteres para dar a conocer información, dentro de una empresa, pero con el pasar del tiempo esta herramienta fue evolucionando hasta convertirse en la primera red de aceptación como apoyo para el periodismo digital. El Periodismo actual se está ayudando de herramientas tecnológicas como el Twitter para poder aportar noticias o acontecimientos que se generan dentro del territorio local, nacional y también a nivel internacional. Los diarios locales como: diario El Tiempo y diario El Mercurio dieron sus inicios a la era digital al mismo tiempo, ambos medios de comunicación poseen sus respectivas versiones electrónicas con las cuales informan a la ciudadanía ecuatoriana y a las personas que viven fuera del país. Los periodistas han aportado con conocimientos y experiencias personales en el manejo de Twitter como herramienta esencial que tiene el periodismo en la actualidad, también están de acuerdo que los periodistas necesitan de la última tecnología para poder sacarle el mayor provecho a esta red social, poseer dispositivos móviles facilita el desempeño periodístico y se puede twittear desde cualquier lugar donde acontecen hechos noticiosos.

Quichua-Spanish language contact in Salcedo, Ecuador: revisiting media lengua syncretic language practices

The purpose of the current thesis is to develop a better understanding of the interaction between Spanish and Quichua in the Salcedo region and provide more information for the processes that might have given rise to Media Lengua, a ‘mixed’ language comprised of a Quichua grammar and Spanish lexicon. Muysken attributes the formation of Media Lengua to relexification, ruling out any influence from other bilingual phenomena. I argue that the only characteristic that distinguishes Media Lengua from other language contact varieties in central Ecuador is the quantity of the overall Spanish borrowings and not the type of processes that might have been employed by Quichua speakers during the genesis of Media Lengua. The results from the Salcedo data that I have collected show how processes such as adlexification, code-mixing, and structural convergence produce Media Lengua-type sentences, evidence that supports an alternative analysis to Muysken’s relexification hypothesis. Overall, this dissertation is developed around four main objectives: (1) to describe the variation of Spanish loanwords within a bilingual community in Salcedo; (2) to analyze some of the prominent and recent structural changes in Quichua and Spanish; (3) to determine whether Spanish loanword use can be explained by the relationship consultants have with particular social categories; and (4) to analyze the consultants’ language ideologies toward syncretic uses of Spanish and Quichua. Overall, 58% of the content words, 39% of the basic vocabulary, and 50% of the subject pronouns in the Salcedo corpus were derived from Spanish. When compared to Muysken’s description of highlander Quichua in the 1970’s, Spanish loanwords have more than doubled in each category. The overall level of Spanish loanwords in Salcedo Quichua has grown to a level between highlander Quichua in the 1970’s and Media Lengua. Similar to Spanish’s lexical influence in Media Lengua, the increase of Spanish borrowings in today’s rural Quichua can be seen in non-basic and basic vocabularies as well as the subject pronoun system. Significantly, most of the growth has occurred through forms of adlexification i.e., doublets, well-established borrowings, and cultural borrowings, suggesting that ‘ordinary’ lexical borrowing is also capable of producing Media Lengua-type sentences. I approach the second objective by investigating two separate phenomena related to structural convergence. The first examines the complex verbal constructions that have developed in Quichua through Spanish loan translations while the second describes the type of Quichua particles that are attached to Spanish lexemes while speaking Spanish. The calquing of the complex verbal constructions from Spanish were employed when speaking standard Quichua. Since this standard form is typically used by language purists, I argue that their use of calques is a strategy of exploiting the full range of expression from Spanish without incorporating any of the Spanish lexemes which would give the appearance of ‘contamination’. The use of Quichua particles in local varieties of Spanish is a defining characteristic of Quichuacized Spanish, spoken most frequently by women and young children in the community. Although the use of Quichua particles was probably not the main catalyst engendering Media Lengua, I argue that its contribution as a source language to other ‘mixed’ varieties, such as Media Lengua, needs to be accounted for in descriptions of BML genesis. Contrary to Muysken’s representation of relatively ‘unmixed’ Spanish and Quichua as the two source languages of Media Lengua, I propose that local varieties of Spanish might have already been ‘mixed’ to a large degree before Media Lengua was created. The third objective attempts to draw a relationship between particular social variables and the use of Spanish loanwords. Whisker Boxplots and ANOVAs were used to determine which social group, if any, have been introducing new Spanish borrowings into the bilingual communities in Salcedo. Specifically, I controlled for age, education, native language, urban migration, and gender. The results indicate that none of the groups in each of the five social variables indicate higher or lower loanword use. The implication of these results are twofold: (a) when lexical borrowing occurs, it is immediately adopted as the community-wide norm and spoken by members from different backgrounds and generations, or (b) this level of Spanish borrowing (58%) is not a recent phenomenon. The fourth and final objective draws on my ethnographic research that addresses the attitudes of syncretic language use. I observed that Quichuacized Spanish and Hispanicized Quichua are highly stigmatized varieties spoken by the country’s most marginalized populations and families, yet within the community, syncretic ways of speaking are in fact the norm. It was shown that there exists a range of different linguistic definitions for ‘Chaupi Lengua’ and other syncretic language practices as well as many contrasting connotations, most of which were negative. One theme that emerged from the interviews was that speaking syncretic varieties of Quichua weakened the consultant’s claim to an indigenous identity. The linguistic and social data presented in this dissertation supports an alternative view to Muysken’s relexification hypothesis, one that has the advantage of operating with well-precedented linguistic processes and which is actually observable in the present-day Salcedo area. The results from the study on lexical borrowing are significant because they demonstrate how a dynamic bilingual speech community has gradually diversified their Quichua lexicon under intense pressure to shift toward Spanish. They also show that Hispanicized Quichua (Quichua with heavy lexical borrowing) clearly arose from adlexification and prolonged lexical borrowing, and is one of at least six identifiable speech styles found in Salcedo. These results challenge particular interpretations of language contact outcomes, such as, ones that depict sources languages as discrete and ‘unmixed.’ The bilingual continuum presented in this thesis shows on the one hand, the range of speech styles that are accessible to different speakers, and on the other hand, the overlapping, syncretic features that are shared among the different registers and language varieties. It was observed that syncretic speech styles in Salcedo are employed by different consultants in varied interactional contexts, and in turn, produce different evaluations by other fellow community members. In the current dissertation, I challenge the claim that relexification and Media Lengua-type sentences develop in isolation and without the influence of other bilingual phenomena. Based on Muysken's Media Lengua example sentences and the speech styles from the Salcedo corpus, I argue that Media Lengua may have arisen as an institutionalized variant of the highly mixed "middle ground" within the range of the Salcedo bilingual continuum discussed above. Such syncretic forms of Spanish and Quichua strongly resemble Media Lengua sentences in Muysken’s research, and therefore demonstrate how its development could have occurred through several different language contact processes and not only through relexification.

Microbiology of chronic suppurative otitis media at Queen Elizabeth Central Hospital, Blantyre, Malawi: A cross-sectional descriptive study

Background Chronic suppurative otitis media (CSOM) is still a significant health problem in developing countries. Therefore, it was pertinent to determine the local Malawian microbiology in order to guide adequate treatment, avoid complications, and provide records for future reference. Aim The study sought to determine the CSOM-causing microorganisms at Queen Elizabeth Central Hospital in Blantyre, Malawi, and establish their relationship signs and symptoms, and with the demographic pattern of the study. Methods This was a hospital-based cross-sectional descriptive study carried out at the ENT outpatient clinic and the Microbiology Department of Queen Elizabeth Central Hospital.The sample comprised 104 patients with unilateral or bilateral active CSOM, who met the inclusion criteria. All patients were evaluated through a detailed history and clinical examination. Pus samples from draining ears were collected by aspiration with a sterile pipette. The specimens were immediately sent for microbiological analysis. Data were analyzed using SPSS.version 20. Results The study found that Proteus mirabilis , Pseudomonas aeruginosa , and Staphylococcus aureus were the most prevalent aerobic bacteria, while Bacteroides spp. and Peptostreptococcus spp. were the commonest anaerobic bacteria causing CSOM. These CSOM-causing microorganisms were predominant among males aged 18 years and below. Some CSOMcausing microorganisms were—significantly more so than the others— characteristically associated with each of the following clinical features: quantity of pus drainage, mode of onset, otalgia, hearing loss, location of tympanic membrane perforation, and mucosal appearance.

Proposta metodológica de criação de um observatório municipal de desporto, na administração pública local

Introdução e Objectivos: As organizações necessitam de dados para tomar decisões, necessitam de elementos que sirvam de base à resolução de problemas ou à formulação de um juízo. Necessitam de um índice, de uma informação objectiva, disponível para qualquer indivíduo a manipular de acordo com o tipo de análise que pretende efectuar. Temos cada vez mais de nos preocupar em estar devidamente informados e estabelecer as relações mais vantajosas para a nossa organização e para o mercado onde actuamos. Irá implicar a utilização de distintos modelos de análise e instrumentos de recolha de dados, de forma a criar uma base de informação útil aos vários organismos (municipais e nacionais), que projectam as políticas desportivas a desenvolver a nível local e nacional. Com a presente investigação, pretendemos propor um Modelo de Observatório do Desporto e das Actividades Complementares associado a um Sistema de Informação Desportivo, que permita ao poder local (Autarquias), proceder ao levantamento, sempre que necessário, das evoluções concelhias do mercado desportivo, para que de uma forma sustentada, criem e desenvolvam políticas desportivas locais que melhor se ajustem à sua realidade e que sirvam de base à criação da Conta Satélite do Desporto. Material e Métodos: O modelo será testado no concelho de Odivelas, inserido na estrutura da CM Odivelas, sendo necessário a inquirição dos clubes do concelho (apenas os clubes com actividade desportiva activa), numa 1ª fase, e numa 2ª fase, as empresas que lhe dão suporte. Os presidentes e directores de ambas as organizações serão os alvos da aplicação do questionário informático e/ou questionário em suporte papel, de acordo com as realidades e características de cada clube e empresa. Os dados recolhidos serão tratados tratados através do software da aplicação informática que foi criada especificamente para o efeito e que permitirá a exportação de dados para outros suportes informáticos. A própria aplicação irá permitir a construção e apresentação de relatórios de acordo com os requisitos solicitados. Resultados: O objectivo geral do estudo foi alcansado com a construção de um Modelo de Observatório Municipal do Desporto, baseado numa proposta de Sector do Desporto e de Conta Satélite do Desporto, tendo por base o estudo de caso na Câmara Municipal de Odivelas. Os objectivos específicos prenderam-se com a construção de instrumentos de recolha de dados para: - identificar os serviços desportivos e de complemento desportivo prestados no concelho de Odivelas; - identificar as organizações que prestam serviços desportivos e de Proposta Metodológica de Criação de um Observatório Municipal de Desporto, na Administração Pública Local complemento ao desporto; Os dados recolhidos terão de ser cruzados com a informação já existente, mas que se encontra dispersa pelas várias estruturas da CMO, de forma a permitir realizar o “levantamento” dos valores económicos gerados pelas actividades desportivas dos clubes, colectividades e empresas prestadoras de serviços e identificar as lacunas de serviços necessários aos clubes e colectividades não existentes no concelho.

El peso de la información de los gobiernos en la prensa española de referencia nacional y local. Análisis de casos de los diarios El País, El Mundo, Sur y Málaga Hoy (2010)

En una etapa especialmente convulsa para la prensa escrita y su modelo de negocio por los efectos de la crisis y el impacto de las nuevas tecnologías, la presente tesis indaga sobre algunas consecuencias que se observan en el comportamiento de los diarios de calidad. El punto de partida medirá la influencia en la determinación de la agenda informativa de cuatro diarios de pago las informaciones que suministran los gobiernos central, autonómico y locales. Los resultados servirán para profundizar en las rutinas profesionales, el uso de las fuentes en relación a las noticias fabricadas por los gabinetes de comunicación y, en general, la calidad de los contenidos que se difunden. Elementos sobre los que gravita la credibilidad. El trabajo pretende contrastar con datos algunas de las percepciones negativas que envuelven e ejercicio de periodismo en España, analizar las posibles causas que se encuentran detrás de ese deterioro y reflejar el sentir de los profesionales y académicos que analizan y afrontan en estos últimos años la evolución de los también denominados periódicos de referencia. Para el desarrollo de esta tesis se ha realizado un análisis cuantitativo de las informaciones publicadas en las secciones más significativas de El País y El Mundo, los dos periódicos de referencia de mayor difusión de España y Sur y Málaga Hoy, diario líder de la provincia de Málaga y uno de los últimos rotativos en papel que han nacido en España. Con la elección se busca estudiar analogías y diferencias en los comportamientos entre rotativos de ámbito nacional y provincial, en los que respecta a la difusión a través de sus páginas de noticias procedentes de la Comunicación de la Administración. La muestra utilizada para el trabajo de campo fueron las noticias publicadas por los cuatro periódicos durante una semana a lo largo de los doce meses del año 2010, en un muestreo aleatorio sistemático. Los resultados se han complementado con un análisis cualitativo en 2015, mediante la fórmula de panel de expertos, con un cuestionario idéntico y una selección vía muestreo opinático, con 28 participantes. Prácticamente la mitad de ellos son directores de periódicos. El resto de los participantes, decanos de media docena de facultades de Ciencias de la Información y la Comunicación de España y académicos estudiosos sobre la materia. Como conclusiones, el análisis cuantitativo revela el excesivo peso de la presencia de los gobiernos en las informaciones principales de secciones como España y Andalucía, así como la facilidad de los gabinetes de prensa de la Administración para condicionar la agenda. El estudio cuantitativo refleja la convicción de la gran mayoría de los participantes de que se da un abuso de información oficial que impacta sobre la credibilidad. Bibliografía. Canel, M.J. Comunicación Política. 2006. Diezhandino, M.P. El periodista en la encrucijada.2012. Cerezuelo B. Periodistas, empresas e instituciones. 2006. Díaz Nosty, B. El déficit mediático. 2005. Vázquez, M.A. Periodismo de declaraciones 2005. Tuchman, G. La producción de la noticia. 1983

Optimization of Wireless Power Transfer via Magnetic Resonance in Different Media

A wide range of non-destructive testing (NDT) methods for the monitoring the health of concrete structure has been studied for several years. The recent rapid evolution of wireless sensor network (WSN) technologies has resulted in the development of sensing elements that can be embedded in concrete, to monitor the health of infrastructure, collect and report valuable related data. The monitoring system can potentially decrease the high installation time and reduce maintenance cost associated with wired monitoring systems. The monitoring sensors need to operate for a long period of time, but sensors batteries have a finite life span. Hence, novel wireless powering methods must be devised. The optimization of wireless power transfer via Strongly Coupled Magnetic Resonance (SCMR) to sensors embedded in concrete is studied here. First, we analytically derive the optimal geometric parameters for transmission of power in the air. This specifically leads to the identification of the local and global optimization parameters and conditions, it was validated through electromagnetic simulations. Second, the optimum conditions were employed in the model for propagation of energy through plain and reinforced concrete at different humidity conditions, and frequencies with extended Debye's model. This analysis leads to the conclusion that SCMR can be used to efficiently power sensors in plain and reinforced concrete at different humidity levels and depth, also validated through electromagnetic simulations. The optimization of wireless power transmission via SMCR to Wearable and Implantable Medical Device (WIMD) are also explored. The optimum conditions from the analytics were used in the model for propagation of energy through different human tissues. This analysis shows that SCMR can be used to efficiently transfer power to sensors in human tissue without overheating through electromagnetic simulations, as excessive power might result in overheating of the tissue. Standard SCMR is sensitive to misalignment; both 2-loops and 3-loops SCMR with misalignment-insensitive performances are presented. The power transfer efficiencies above 50% was achieved over the complete misalignment range of 0°-90° and dramatically better than typical SCMR with efficiencies less than 10% in extreme misalignment topologies.

MARS (Media Asset Retrieval System): Puget Sound’s Digital Archive

The MARS (Media Asset Retrieval System) Project is the collaborative effort of public broadcasters,libraries and schools in the Puget Sound region to create a digital online resource that provides access to content produced by public broadcasters via the public libraries. Convergence ConsortiumThe Convergence Consortium is a model for community collaboration, including organizations such as public broadcasters, libraries, museums, and schools in the Puget Sound region to assess the needs of their constituents and pool resources to develop solutions to meet those needs. Specifically, the archives of public broadcasters have been identified as significant resources for the local communities and nationally. These resources can be accessed on the broadcasters websites, and through libraries and used by schools, and integrated with text and photographic archives from other partners.MARS’ goalCreate an online resource that provides effective access to the content produced locally by KCTS (Seattle PBS affiliate) and KUOW (Seattle NPR affiliate). The broadcasts will be made searchable using the CPB Metadata Element Set (under development) and controlled vocabularies (to be developed). This will ensure a user friendly search and navigation mechanism and user satisfaction.Furthermore, the resource can search the local public library’s catalog concurrently and provide the user with relevant TV material, radio material, and books on a given subject.The ultimate goal is to produce a model that can be used in cities around the country.The current phase of the project assesses the community’s need, analyzes the current operational systems, and makes recommendations for the design of the resource.Deliverables• Literature review of the issues surrounding the organization, description and representation of media assets• Needs assessment report of internal and external stakeholders• Profile of the systems in the area of managing and organizing media assetsfor public broadcasting nationwideActivities• Analysis of information seeking behavior• Analysis of collaboration within the respective organizations• Analysis of the scope and context of the proposed system• Examining the availability of information resources and exchangeof resources among users

Microwave-assisted synthesis and local analyses of positive insertion electrodes for Li-ion batteries

Efficient energy storage holds the key to reducing waste energy and enabling the use of advanced handheld electronic devices, hydrid electric vehicles and residential energy storage. Recently, Li-ion batteries have been identified and employed as energy storage devices due to their high gravimetric and volumetric energy densities, in comparison to previous technologies. However, more research is required to enhance the efficiency of Li-ion batteries by discovering electrodes with larger electrochemical discharge capacities, while maintaining electrochemical stability. The aims of this study are to develop new microwave-assisted synthesis routes to nanostructured insertion cathodes, which harbor a greater affinity for lithium extraction and insertion than bulk materials. Subsequent to this, state-of-the-art synchrotron based techniques have been employed to understand structural and dynamic behaviour of nanostructured cathode materials during battery cell operation. In this study, microwave-assisted routes to a-LiFePO4, VO2(B), V3O7, H2V3O8 and V4O6(OH)4 have all been developed. Muon spin relaxation has shown that the presence of b-LiFePO4 has a detrimental effect on the lithium diffusion properties of a-LiFePO4, in agreement with first principles calculations. For the first time, a-LiFePO4 nanostructures have been obtained by employing a deep eutectic solvent reaction media showing near theoretical capacity (162 mAh g–1). Studies on VO2(B) have shown that the discharge capacity obtained is linked to the synthesis method. Electrochemical studies of H2V3O8 nanowires have shown outstanding discharge capacities (323 mAh g–1 at 100 mA g–1) and rate capability (180 mAh g–1 at 1 A g–1). The electrochemcial properties of V4O6(OH)4 have been investigated for the first time and show a promising discharge capacity of (180 mAh g–1). Lastly, in situ X-ray absorption spectroscopy has been utilised to track the evolution of the oxidation states in a-LiFePO4, VO2(B) and H2V3O8, and has shown these can all be observed dynamically.

ORGANIZACIÓN TERRITORIAL DEL TURISMO ALTERNATIVO EN LA ZONA MEDIA DEL ESTADO DE SAN LUIS POTOSÍ, MÉXICO: RETOS Y PERSPECTIVAS

Ubicado en la porción central de México, la zona Media del estado de San Luis Potosí se incorpora a la oferta de espacios turísticos con los que se busca diversificar la economía regional mediante la política pública vigente en la entidad. Dicha zona, alberga atractivos turísticos concurridos por visitantes nacionales e internacionales, como la Laguna de la Media Luna; no obstante, existen otros espacios con potencial que aún no han sido considerados como parte de las estrategias programadas. En este contexto, el trabajo identifica los retos y perspectivas asociados al impulso del turismo alternativo en la zona Media, toda vez que en Plan Estatal de Desarrollo, 2009-2015 se contempla que la entidad sea un destino líder en turismo de naturaleza y cultural para los próximos años. La metodología empleada involucra trabajo de campo en 6 municipios de la región, aplicación de entrevistas con la población local, prestadores de servicios y autoridades del sector turismo. Posteriormente se realizó un análisis FODA con el que se identifican las fortalezas, debilidades, oportunidades y amenazas que, acompañadas con una propuesta de una ruta ecoturística, se representaron en mapas temáticos que revelan la organización territorial que subyace en la planeación turística.

An Efficient Protocol For The Generation Of Monocyte Derived Dendritic Cells Using Serum-free Media For Clinical Applications In Post Remission Aml Patients.

Protocols for the generation of dendritic cells (DCs) using serum as a supplementation of culture media leads to reactions due to animal proteins and disease transmissions. Several types of serum-free media (SFM), based on good manufacture practices (GMP), have recently been used and seem to be a viable option. The aim of this study was to evaluate the results of the differentiation, maturation, and function of DCs from Acute Myeloid Leukemia patients (AML), generated in SFM and medium supplemented with autologous serum (AS). DCs were analyzed by phenotype characteristics, viability, and functionality. The results showed the possibility of generating viable DCs in all the conditions tested. In patients, the X-VIVO 15 medium was more efficient than the other media tested in the generation of DCs producing IL-12p70 (p=0.05). Moreover, the presence of AS led to a significant increase of IL-10 by DCs as compared with CellGro (p=0.05) and X-Vivo15 (p=0.05) media, both in patients and donors. We concluded that SFM was efficient in the production of DCs for immunotherapy in AML patients. However, the use of AS appears to interfere with the functional capacity of the generated DCs.