Zur Volkskunde der Juden : mit 1 Karte üb. d. Verbreitung d. Juden in Mitteleuropa

von Richard Andree

Heterologous expression from the human D-Loop in organello

We report the expression of a linear reporter construct in isolated human mitochondria. The reporter construct contained the entire human D-Loop with adjacent tRNA (MTT) genes (mt.15956-647), the human ND1 gene with an in frame GFP gene and adjacent endogenous MTT genes and heterologous rat MTT genes. Natural competence of isolated human mitochondria of HepG2 cells was used to import reporter constructs. The import efficiency of various fluorescently labelled PCR-generated import substrates in the range of 250bp up to 3.5kb was assessed by quantitative PCR and evaluated by confocal microscopy. Heterologous expression of the imported construct was confirmed at RNA level by a circular RNA (cRNA)-RT-PCR assay for the expression of tRNAs and by in organello [α-(32)P]-UTP labelling and subsequent hybridisation to reporter-specific sequences for monitoring mRNA expression. Heterologous expression of rat mitochondrial tRNA(Leu(UUR)) (rMT-TL1) was confirmed by co-/post-transcriptional trinucleotide (CCA) addition. Interestingly, the rat-specific MT-TL1 was correctly processed in isolated human mitochondria at the 3' end, but showed an aberrant 5' end processing. Correct 3' end processing of the heterologous expressed mitochondrial rat tRNA(Ser2) (MT-TS2) was detected. These findings demonstrate the feasibility of genetic manipulation of human mitochondria, providing a tool for characterisation of cis-acting elements of the human mitochondrial genome and for the study of human mitochondrial tRNA processing in organello.

Unmaßgebliche Vorschläge, als Instruktion für den Herrn Sattlermeister Georg Hollenbach, Abgeordneter der mittelfränkischen Kreishauptstadt Ansbach, zu der am 15. Juli d. Js. in Frankfurt a/M. zusammentretenden Versammlung deutscher Gewerbetreibenden ertheilt: Bei der Besprechung des Comité's für die Gewerbs-Congreßbeschickung zu Frankfurt ...

Welsch (Projektbearbeiter): Verhaltensmaßregeln für den als Vertreter Ansbachs in den 'Deutschen Handwerker- und Gewerbe-Congreß' nach Frankfurt entsandten Sattlermeister Hollenbach

Kundmachung: Der Gemeinderath der Stadt Wien hat durch Beschluß vom 6. d. M. in Anbetracht der traurigen Verhältnisse zu Folge deren die Möglichkeit mangelte ...

Welsch (Projektbearbeiter): Einstellung der finanziellen Unterstützung mittelloser Personen mit dem 16. November 1848 unter Hinweis auf die überwundene Stockung des Wirtschaftslebens; für Arbeitsunfähige ist die Armenpflege zuständig. Die Möglichkeit einer Anstellung bei öffentlichen Arbeiten, sofern es die klimatischen Bedingungen sowie die finanziellen Mittel der Stadt erlauben, besteht im Falle desjenigen Personenkreises, der auf dem freien Markt keine Beschäftigung findet

Real-time analysis of δ13C- and δD-CH4 in ambient air with laser spectroscopy: method development and first intercomparison results

In situ and simultaneous measurement of the three most abundant isotopologues of methane using mid-infrared laser absorption spectroscopy is demonstrated. A field-deployable, autonomous platform is realized by coupling a compact quantum cascade laser absorption spectrometer (QCLAS) to a preconcentration unit, called trace gas extractor (TREX). This unit enhances CH4 mole fractions by a factor of up to 500 above ambient levels and quantitatively separates interfering trace gases such as N2O and CO2. The analytical precision of the QCLAS isotope measurement on the preconcentrated (750 ppm, parts-per-million, µmole mole−1) methane is 0.1 and 0.5 ‰ for δ13C- and δD-CH4 at 10 min averaging time. Based on repeated measurements of compressed air during a 2-week intercomparison campaign, the repeatability of the TREX–QCLAS was determined to be 0.19 and 1.9 ‰ for δ13C and δD-CH4, respectively. In this intercomparison campaign the new in situ technique is compared to isotope-ratio mass spectrometry (IRMS) based on glass flask and bag sampling and real time CH4 isotope analysis by two commercially available laser spectrometers. Both laser-based analyzers were limited to methane mole fraction and δ13C-CH4 analysis, and only one of them, a cavity ring down spectrometer, was capable to deliver meaningful data for the isotopic composition. After correcting for scale offsets, the average difference between TREX–QCLAS data and bag/flask sampling–IRMS values are within the extended WMO compatibility goals of 0.2 and 5 ‰ for δ13C- and δD-CH4, respectively. This also displays the potential to improve the interlaboratory compatibility based on the analysis of a reference air sample with accurately determined isotopic composition.

Aus einem Schreiben des Herrn D. Fürst in Leipzig vom 5. Februar 1837

Julius Fürst

Pièces d'harmonie in G No. 2 : pour deux clarinetts in A, deux cors, deux fagotts, flauto oblie. et serpento

composée par During, chef de musique à la 108me fr Brigade

Bericht über die bis 145. April d. J. in der Anstalt eingegangenen Meldungen der Auskunftsstellen für Pflanzenschutz und deren Vertrauensmänner

Hiltner

Robert Hutt, Rollenbild, in: Giuseppe Verdi: Aida

Signatur des Originals: S 36/F04644

Robert Schumann-Denkmal in Bonn

Signatur des Originals: S 36/F06457

Otto Fanger als Robert, Rollenbild, Hüftbild, in: Giacomo Meyerbeer: Robert le diable, Frankfurt a. M., 1915

Signatur des Originals: S 36/F11534

Otto Fanger als Robert, Rollenbild, Brustbild, in: Giacomo Meyerbeer: Robert le diable

Signatur des Originals: S 36/F11535

Robert Schumanns Geburtshaus in Zwickau

Signatur des Originals: S 36/G02688