Platelet Redox Balance in Diabetic Patients with Hypertension improved by n-3 fatty acids

OBJECTIVE:
Patients with type 2 diabetes mellitus (T2DM) are at increased risk of developing cardiovascular disease, largely as a result of defective production of cardioprotective nitric oxide and a concomitant rise in oxidative stress. Dietary interventions that could reverse this trend would be extremely beneficial. Here we investigated whether dietary n-3 polyunsaturated fatty acid (n-3 PUFA) supplementation positively affected platelet nitroso-redox imbalance.
RESEARCH DESIGN AND METHODS:
We randomized hypertensive T2DM patients (T2DM HT; n = 22) and age-and-sex matched hypertensive study participants without diabetes (HT alone; n = 23) in a double-blind, crossover fashion to receive 8 weeks of n-3 PUFAs (1.8 g eicosapentaenoic acid and 1.5 g docosahexaenoic acid) or identical olive oil capsules (placebo), with an intervening 8-week washout period. Platelet nitrite and superoxide were measured and compared before and after treatment; 8-isoprostane was determined by ELISA and subcellular compartmentalization of the NAD(P)H oxidase subunit p47-phox examined by Western blotting.
RESULTS:
The n-3 PUFA supplementation reduced 8-isoprostane and superoxide levels in platelets from T2DM HT, but not HT alone, participants, without effect on nitrite production. This coincided with a significant decrease in p47-phox membrane localization and a similar reduction in superoxide to that achieved with apocynin. At baseline, a subcohort of T2DM HT and HT alone participants showed evidence of nitric oxide synthase (NOS)-derived superoxide production, indicating defective enzymatic activity. This was reversed significantly in T2DM HT participants after treatment, demonstrating improved NOS function.
CONCLUSIONS:
Our finding that n-3 PUFAs diminish platelet superoxide production in T2DM HT patients in vivo suggests a therapeutic role for these agents in reducing the vascular-derived oxidative stress associated with diabetes.

Updated systematic review and meta-analysis of the effects of n-3 long-chain polyunsaturated fatty acids on depressed mood

Background: The debate over a role for n-3 long-chain polyunsaturated fatty acids (n-3 PUFAs) in depressed mood continues.

Electron-impact excitation of Li to high principal quantum numbers

The time-dependent close-coupling method is used to calculate electron-impact excitation cross sections for the Li(2s)--{\textgreater}Li(nl) and Li(2p)--{\textgreater}Li(nl) transitions at incident energies just above the ionization threshold. The implementation of the time-dependent close-coupling method on a nonuniform lattice allows the study of continuum-coupling effects in excitations to high principal quantum number, i.e., n{\textless}=10. Good agreement is found with R-matrix with pseudostates calculations, which also include continuum-coupling effects, for excitations to low principal quantum number, i.e., n{\textless}=4. Poor agreement is found with standard distorted-wave calculations for excitations to all principal quantum numbers, with differences still at the 50% level for n=10. We are able to give guidance as to the accuracy expected in the n3 extrapolation of nonperturbative close-coupling calculations of low n cross sections and rate coefficients.

Acesso aos Cuidados de Saúde: um direito em risco?. Relatório Primavera 2015

Em Portugal a saúde tem a dignidade de ser considerada na Constituição da República (CR), no capítulo dos direitos e deveres sociais (Capítulo II). Aí se afirma que “todos têm direito à proteção da saúde e o dever de a defender e promover” (CR, artº 64, nº 1), balanceando-se assim as responsabilidades do Estado, através do dever de proteção, com as responsabilidades individuais, através do dever de defender e promover a saúde. O Estado assume o seu dever garantindo “o acesso de todos os cidadãos, independentemente da sua condição económica” (CR, artº 64, nº3, al. a). O acesso aos cuidados de saúde constitui-se assim como a forma de o Estado garantir aos cidadãos o direito à saúde.

A influência da viscosidade dos solos argilosos na consolidação do dique nº 3 da Represa de Lebrija

Dissertação de mestrado, Engenharia Civil (Construção), Instituto Superior de Engenharia, Universidade do Algarve, 2014

N.C.R.F. 3: adopção pela primeira vez das Normas Contabilísticas e de Relato Financeiro

Dissertação para obtenção do Grau de Mestre em Auditoria

A biocomposite of collagen nanofibers and nanohydroxyapatite for bone regeneration

This work aims to design a synthetic construct that mimics the natural bone extracellular matrix through innovative approaches based on simultaneous type I collagen electrospinning and nanophased hydroxyapatite (nanoHA) electrospraying using non-denaturating conditions and non-toxic reagents. The morphological results, assessed using scanning electron microscopy and atomic force microscopy (AFM), showed a mesh of collagen nanofibers embedded with crystals of HA with fiber diameters within the nanometer range (30 nm), thus significantly lower than those reported in the literature, over 200 nm. The mechanical properties, assessed by nanoindentation using AFM, exhibited elastic moduli between 0.3 and 2 GPa. Fourier transformed infrared spectrometry confirmed the collagenous integrity as well as the presence of nanoHA in the composite. The network architecture allows cell access to both collagen nanofibers and HA crystals as in the natural bone environment. The inclusion of nanoHA agglomerates by electrospraying in type I collagen nanofibers improved the adhesion and metabolic activity of MC3T3-E1 osteoblasts. This new nanostructured collagen–nanoHA composite holds great potential for healing bone defects or as a functional membrane for guided bone tissue regeneration and in treating bone diseases.

La caza de cachalote en el Perú. Estadísticas de captura para los años 1947 - 1961 y un intento de analizar las condiciones de la población en el periodo 1954 - 1961

Presenta datos basicos del numero cachalotes capturados cada años desde 1947 en la region del Pacifico Sureste. Datos sobre el esfuerzo y la captura por unidad de esfuerzo de las operaciones de caza de Pisco para el periodo 1954-1961 y de Paita para el 1957-1961. Ademas, la estructura de la poblaciòn, la abundancia de la poblacion y su relacion con el esfuerzo.

Exploración bio - oceanológica básica del área Callao - Punta Aguja Crucero E-6503

Describe las condiciones bio-oceanológicas encontradas en la región Callao -Punta Aguja, durante el Crucero E-6503, admitiendo que hubo cambios en el régimen térmico de nuestro mar, ocasionados por el avance hacia el sur de aguas ecuatoriales con temperaturas de 26º -24º C y salinidades de 34.6 -34.1 º/00. Por otro lado demuestra que la corriente costanera se presentó en forma de una franja estrecha, variable de 10-40 millas de la costa con temperaturas de 22º - 19º C y salinidades alrededor de 34.9 º/00. Estas aguas presentaron su mayor ensanchamiento al norte de Huarmey hasta Pimentel.

Estudio del Camaron en el Perú 1975-1976

Como resultado de un estudio de aproximadamente dos años en cinco ríos de la Costa Central y Sur del Perú, se presenta información biológico-pesquera de Cryphiops caementarius Molina 1872 (Natantia, Palaemonidae), comprendiendo aspectos de su distribución, bionomía y ciclo vital, población, explotación y manejo. Se hace una descripción y análisis de cada uno de estos aspectos, así como de las condiciones ambientales presentes en los diferentes ríos estudiados.

Ictioplancton de la costa peruana

Los resultado que se obtuvieron provienen de 41 exploraciones, efectuados de 1966 a 1974 por el Instituto del Mar del Perú en el Programa de Ictioplancton. Se presenta la composición de la fauna íctica y la abundancia relativa de especies y grupos de peces en litoral Peruano.