208 resultados para CHONDROITIN SULPHATE


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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A remoção da smear layer é um procedimento importante para a regeneração periodontal e para o tratamento da sensibilidade dentinária. Diversos tratamentos tem sido realizados para sua remoção, porém, mostrando resultados conflitantes ou insatisfatórios. No presente estudo nos propomos a analisar através do microscópio eletrônico de varredura a eficiência dos detergentes: lauril sulfato de sódio, Plax e de mamona assim como o EDTA 24% na remoção da smear layer. Todos os produtos testados foram aplicados com bolinha de algodão, renovada a cada 30 segundos, a qual era esfregada na superfície radicular por diferentes períodos. As fotomicrografias foram analisadas através da aplicação de um índice de remoção da smear layer por três examinadores calibrados e os resultados analisados estatisticamente. De acordo com nossos resultados pudemos chegar as seguintes conclusões: 1- a instrumentação com curetas de Gracey provocou a formação da smear layer; 2- o detergente lauril sulfato de sódio não removeu a smear layer, tendo sido o pior resultado entre os detergentes testados; 3- Os detergentes Plax e de mamona mostraram resultados semelhantes entre si e superiores ao controle negativo e ao lauril sulfato de sódio, porém, não removeram a smear layer satisfatoriamente; 4- O EDTA 24% neutro mostrou ser eficiente para a remoção da smear layer.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Bacterial cellulose (BC) has become established as a remarkably versatile biomaterial and can be used in a wide variety of scientific applications, especially for medical devices. In this work, the bacterial cellulose fermentation process is modified by the addition of chondroitin sulfate and hyaluronic acid (1% w/w) to the culture medium before the bacteria is inoculated. Besides, biomimetic precipitation of calcium phosphate of biological interest from simulated body fluid on bacterial cellulose was studied. Chondroitin sulfate and hyaluronic acid effects in bacterial cellulose were analyzed using transmission infrared spectroscopy (FTIR), XRD (X-ray diffraction) and scanning electron microscopy (SEM). FTIR analysis showed interaction between bacterial cellulose nanobiocomposites and calcium phosphate. XRD demonstrated amorphous calcium phosphate, carbonated apatite and calcium chloride on bacterial cellulose nanobiocomposites. Monocalcium phosphate monohydrate phase formation [Ca(H2PO4)(2)center dot H2O] are here attested by FTIR, XRD and Ca/P relation.

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The time, dose and applied nutrients in corn have a direct effect on its productivity. Therefore, the objective was to study the application of N and S in corn as ammonium sulfate, in succession to wheat and oats and evaluate different forms of fertilizer management. The experiment was conducted in a randomized block design in Oxisol (Hapludox). The five treatments with N, at a dose of 120 kg ha(-1) were applied in 20 plots (5x4), according to the management of fertilizer: T1-N (120 kg ha(-1)) full at sowing, T2-N (120 kg ha(-1)) total coverage; T3-N (40 kg ha(-1)) at sowing and N (80 kg ha(-1)) in coverage; T4-N advance in wheat sowing and sowing oats (120 kg ha(-1)), T5-(control). The S doses were corresponding to their concentrations in the fertilizer. Only wheat received a dose of 24 kg N ha(-1) at sowing all plots and oats received 24 kg N ha(-1) at sowing only the portions related to treatment with anticipation of corn N (T4). We evaluated the biomass production of winter crops (oats and wheat), according to the fertilization at sowing, and also the influence of winter crops and management of ammonium sulfate, the corn yield. The oats produced more dry matter in relation to wheat, positively influencing the corn yield, regardless of fertilizer management. The anticipation of ammonium sulfate, the sowing of oats, was favorable to corn yield, equating to other forms of management of fertilizer. Rotation corn and oats, forms management, ammonium sulphate, at seeding, topdressing or applied in split were equally efficient in corn yields.

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This study aims to determine surface skin doses in dogs (with suspected pulmonary metastasis) submitted to chest X-rays using the technique of thermoluminescence dosimetry. Twenty seven exams from different dogs were performed at the Faculdade de Medicina Veterinária e Zootecnia da Universidade Estadual Paulista (FMVZ-UNESP/Botucatu). The doses were evaluated using thermoluminescent dosimeters of calcium sulphate doped with dysprosium (CaSO4:Dy) produced by the Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN). The pulmonary metastasis exams are carried out in three projections, one dorsal-ventral and two lateral-lateral. During the procedures the projection thicknesses and source-skin surface distances were registered. To simulate the dog phantom the dosimeters were positioned in a cubic simulator (30x30x30 cm) of polymethylmethacrylate (PMMA) filled with water and irradiated according to the parameters of projections with the X-ray energies ranging from 45 to 70 kV. To estimate the surface skin dose the dose-response curves were obtained for X-ray energies of 50 and 70 kV using the diagnostic X-ray beam machine of the Instruments Calibration Laboratory of IPEN for doses of 1.5, 2.0, 2.5, 3.5 and 4.0 mGy. The main difficulty of this work was the dog immobilization that is reflected in poor-quality diagnostic imaging and, consequently, demands the repetition of the exams, which contributes to the increase of the doses received by the animals being studied and the clinical staff. The doses evaluated in this type of procedure are between 0.43 and 4.22 mGy. This research has shown to be extremely important for the assessment of doses involved in veterinary diagnostic radiology procedures, and as a parameter in the individual monitoring of pet’s owners who assist the animal positioning and occupationally exposed workers of the Department of Veterinary Radiology

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This study investigated the effect of inexpensive carbon and nitrogen sources on enzyme production by Myceliophthora thermophila M.7.7 in solid-state fermentation. Three kinds of lignocellulosic waste (corn straw, sugarcane bagasse and sugarcane straw) and six nitrogen sources (urea, calcium nitrate, analytical ammonium sulphate, yeast extract, agricultural fertilizer NPK 20-05-20 and fertilizing grade ammonium sulphate) were tested. Some physical-chermical parameters of the fermentation, such as temperature, initial pH and moisture content of the substrate on enzyme production, were evoluated. The maximum activities of xylanase (446.9 U/ml) endoglucanase (94.7 U/ml) and beta-glucosidase (2.8 U/ml) were observed in a mixture of corn straw and wheat bran (1:1 w/w) as the carbon source using fertilizer grade ammonium sulphate as the nitrogen source. This production occurred for an incubation period of 96 h, at 40°C, with initial moisture content of 70% and pH 5.0. These results have significant interest since they could be used for the future production of enzymes in a low-cost industrial process.