Screening and determination of sulphonamide residues in bovine milk samples using a flow injection system


Autoria(s): Bedatty Fernandes, Flavio Cesar; Silva, Aline Santana; Rufino, Jose Luiz; Pezza, Helena Redigolo; Pezza, Leonardo
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

18/03/2015

18/03/2015

01/01/2015

Resumo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A new, simple, rapid and sensitive flow injection spectrophotometric method was developed for the screening and determination of sulphonamides in bovine milk samples. The method is based on the condensation of sulphathiazole, sulphamethazine, and sulphadimethoxine with p-dimethylaminobenzaldehyde (p-DAB) in acid medium, in the presence of sodium dodecyl sulphate (SDS), producing a yellow compound (lambda(max) = 465 nm). Optimisation of the experimental parameters was performed using a multivariate methodology. The linear range was 90-500 mu g/L and the limits of detection and quantification were in the ranges 25-29 mu g/L and 84-88 mu g/L, respectively. The procedure was applied for the determination of sulphonamide antibiotics in bovine milk samples submitted to a prior extraction procedure based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology. Recoveries of 60.5-70.5% were achieved for milk samples spiked with 0.09, 0.1, 0.2, and 0.3 mu g/g of each sulphonamide. (C) 2014 Elsevier Ltd. All rights reserved.

Formato

309-315

Identificador

http://dx.doi.org/10.1016/j.foodchem.2014.06.034

Food Chemistry. Oxford: Elsevier Sci Ltd, v. 166, p. 309-315, 2015.

0308-8146

http://hdl.handle.net/11449/116551

10.1016/j.foodchem.2014.06.034

WOS:000342654200042

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Food Chemistry

Direitos

closedAccess

Palavras-Chave #Sulphonamides #p-Dimethylaminobenzaldehyde #Spectrophotometry #Screening #Flow injection analysis #Milk
Tipo

info:eu-repo/semantics/article