216 resultados para Bioguided fractionation


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In order to enable the reuse of the large surplus volume of petroleum produced water for irrigation of crops inedible, the quality evaluation of this water is very important. This work aimed to evaluate the effectiveness of the diffusive gradient in thin films technique (DGT) for the determination of labile Cu (II), Mn (II) and Zn (II) in petroleum produced water destined to reuse in agriculture. Samples were collected at the exit of the wastewater treatment plant (WWTP) (after separation oil/water and flotation) of a Petrobrás onshore production region. Basically, the laboratory experiments with DGT devices involved the evaluation of the behavior of the main variables of the technique for determination of analytes in the samples (diffusion coefficients, immersion time, fractionation of organic and inorganic species, and performance of diffusion gels with different porosities, among others). Also, experiments were conducted based on the solid phase extraction (SPE) protocol with Chelex- 100 resin using previously established protocols to support the study. During the project the possibility of in situ immersions in water treatment plants was evaluated. The DGT technique presented satisfactory results for determination of Mn in produced water, and can be used for in situ determinations. However, the results obtained for Cu and Zn show the need of additional studies

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The increase of the antimicrobial resistance and its propagation around the world are the biggest threats to the public health care and to the treatment of diseases caused by microorganisms. Nowadays the antimicrobial resistance has increased abruptly. The essential oils are volatile and aromatic compounds derived from parts of plants as flowers, leafs, fruits, seeds, roots, sprouts, among others. The activity of extracts and essential oils of several plant species have been recognized and studied by empirical methods since a long time, but its antimicrobial activities were confirmed recently. Medicinal plants are used in folk medicine as medicines, antibiotic, analgesic, sedative and anti-inflammatory. The use of medicinal plants like source of medicines is an alternative of therapeutics for diseases treatment. In Brazil, studies with this goal are very important, once medicinal plants have been used as a choice of treatment and prevention of infections and diseases in health areas. Considering the fact that some products from medicinal plants have antimicrobial properties it is expected that using screening programs, new potential medicaments could be developed. Otherwise, scientific researches focused on determining therapeutic potential of plants are limited, there are lack of scientific studies which confirms the potential antibiotics properties of a large number of plants. The aim of the present study is determinate the antimicrobial activity of 10 medicinal species belonging to CPMA - Collection of Medicinal and Aromatic Plants from CPQBA/UNICAMP. The minimal inhibitory (MIC) and minimal bactericidal or fungicidal concentration (MBC) will be determined against the bacteria Escherichia coli, Pseudomonas aeruginosa, Salmonella choleraesuis, Staphylococcus aureus and the yeast Candida albicans. Furthermore, will be conducted chemical identification and fractionation of essential oils and extract with better activity

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Pós-graduação em Agronomia (Ciência do Solo) - FCAV

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Liquid-liquid extraction is utilized for purification of biomolecules by Aqueous Biphasic Systems (ABS), since this process does not damage the biotechnological potential of these compounds. In this work, using the free software Scilab®, the fractionated liquid-liquid extraction was studied aiming a lipase (target enzyme) partition in a water + PEG + DEX system. Lisozime was considered as contaminant. As computer simulations has been extensively used when a first estimation of technical feasibility of process is desired, this work demonstred that the system is viable for recovery the 80 % till 90 % the target enzyme, but should be noted that the trial aimed only the fractionation of the enzyme target of a contaminant, not its concentration, which should be done by another process.

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Pós-graduação em Agronomia (Agricultura) - FCA

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Pós-graduação em Geociências e Meio Ambiente - IGCE

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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