Metalloproteomic profile determination of muscle samples from nile Tilapia (Oreochromis niloticus) using AAS and ESI-MS/MS after 2D-PAGE separation
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
21/10/2015
21/10/2015
01/02/2015
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Resumo |
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Processo FAPESP: 2011/14902-0 Processo FAPESP: 2010/51332-5 This study evaluated the metalloproteomic profile of muscle tissue samples from Nile tilapia (Oreochromis niloticus). Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) methods was used for protein fractionation and identification based on image analysis. Determination of Ca, Cu, Fe, Mn and Zn was performed in the protein spots by flame and/or graphite furnace atomic absorption spectrometry (FAAS, GFAAS), the protein spots were characterized by electrospray ionization-tandem mass spectrometry (ESI-MS/MS). FAAS and GFAAS determinations have indicated the presence of calcium in seven protein spots, iron in only one spot, manganese in six spots, and zinc in two spots. Of the seventeen protein spots where the presence of metal ions was detected, ten were characterized by ESI-MS/MS. |
Formato |
239-246 |
Identificador |
Journal Of The Brazilian Chemical Society, v. 26, n. 2, p. 239-246, 2015. 0103-5053 http://hdl.handle.net/11449/128655 http://dx.doi.org/10.5935/0103-5053.20140260 S0103-50532015000200239 WOS:000351850100005 S0103-50532015000200239.pdf |
Idioma(s) |
eng |
Publicador |
Soc Brasileira Quimica |
Relação |
Journal Of The Brazilian Chemical Society |
Direitos |
openAccess |
Palavras-Chave | #Metalloproteins #ESI-MS/MS #2D-PAGE |
Tipo |
info:eu-repo/semantics/article |