Direcionamento de peptídeos tolerogênicos da proteína de choque térmico 60 para células dendríticas utilizando anticorpos anti-DEC-205

A indução de tolerância imunológica em situações altamente desejadas como no transplante e em doenças autoimunes permanece um grande desafio para pesquisa científica de tradução. Nesse contexto, o estudo das proteínas do choque térmico (HSPs) e seus peptídeos vêm trazendo informações relevantes sobre o controle da reposta imune. Dados da literatura mostram que alguns de seus peptídeos apresentam propriedades imunorreguladoras, como os peptídeos N3 e N7 da HSP60. Além disso, tem se mostrado que a apresentação de antígenos em contextos específicos por células dendríticas (DCs) pode favorecer o estabelecimento da tolerância imunológica. Dessa forma, o direcionamento dos peptídeos tolerogênicos da HSP60, N3 e N7, in vivo, para DCs, com o intuito de essas células apresentarem esses peptídeos em um contexto imunorregulador, pode induzir um estado de tolerância. Assim, nesse trabalho, tivemos como objetivo a produção de anticorpos (Acs) contra o receptor DEC-205 de DCs conjugados com os peptídeos N3 e N7 da HSP60. Esses Acs, ao se ligarem ao DEC-205 nas DCs, podem ser fagocitados, processados e por fim apresentados a células T em um contexto imunorregulador. Para tal, foram realizadas transfecções de células HEK-293T e CHO com plasmídeos codificando as cadeias leve e pesada dos respectivos Acs a fim de se obter essas proteínas recombinantes. Podemos observar que as células HEK apresentaram uma produção mais eficiente dos Acs quando comparadas com as células CHO (120 vs 30 ng/ml, respectivamente), apesar disso a produção dos Acs ficou abaixo do esperado impossibilitando a realização ensaios in vivo. Além disso, realizamos um ensaio de ligação do Ac anti-DEC-N7 a superfície de DCs e observamos que os Acs produzidos apresentam capacidade de se ligarem a superfície dessas células. Concluímos que os anticorpos recombinantes anti-DEC-205 são capazes... (Resumo completo, clicar acesso eletrônico abaixo)

Otimização de procedimentos de controle de qualidade em equipamentos radiográficos convencionais do Instituto de Eletrotécnica e Energia - USP

Radiography is currently an important method of diagnosis, both medical, dental and veterinary. The image in this type of survey is obtained using an X-ray beam, where the radiologist can possibly view structures of interest. It isn't always possible to get the desired images due to various factors, such as equipment limitations. The Administrative Law 453/98, the State Resolution SS 625/94, and other standards require testing and quality control acceptance limits that guarantee a good performance of the equipment for the security and quality of care, giving service users greater effectiveness in exams. This study were performed in the accompaniment of testing procedures for quality control established by Administrative Law 453/98 in several X-ray equipment to make a comparison and optimization in the descriptions of the procedures used by the Institute for Electrical Energy and the University of São Paulo (IEE / USP / SP).The optimization of the procedures were performed with the aid of a current literature, the Resolution 453/98, State Resolution SS 625/94 and other international standards. On this basis it was possible to observe the importance of regular monitoring of tests for an update, following the technological development of instruments used in the service

A discursivização em Wall.E: sociedade, tecnologia e poder

This article consists in a discourse analysis of the feature film in animation Wall.e produced by Pixar in association to Disney in 2008. Pixar has been known to produce films that contain various discursive levels, making its products appealing not only for children, but also for adults. However, Wall-e proves to be a masterpiece in Pixar carrier, because it is the one which focuses on the analysis and critique of contemporary society associated with technological development. For this, the plot has as its starting point the question of sustainability and unbridled consumerism. But the film expands the debate, tracing the relationship between technology and humanity, discussing how one affects the other. The project therefore aims to show how the animation works such concepts and constructs his discourse. To this end, the work seeks to identify the signs that make up the discourse and draw the intertextuality between WALL.E and other works that also discuss the same elements such as Stanley Kubrick‟s 2001 - A Space Odyssey (1968)

Avaliação da segurança das prescrições médicas de usuários de insulina: estudo piloto

A prescrição médica de insulina e outros medicamentos pode apresentar comprometimento de sua segurança, diante de problemas envolvendo ilegibilidade e ausência de informações. Como consequência instala-se falhas na comunicação e no entendimento do conteúdo prescrito, o que pode ocasionar um erro de medicação com prejuízo terapêutico. Diante disto, o presente trabalho, parte integrante da pesquisa de doutorado “Desenvolvimento e avaliação de um protocolo de atenção farmacêutica intensiva com metodologia educacional de empoderamento para adultos com Diabetes Mellitus tipo 2 – estudo translacional fase 1”, desenvolvido por Drª . Silvia F. L. Cavalheiro, sob orientação da Profª . Drª . Chung Man Chin, teve como objetivo avaliar a segurança das prescrições médicas contendo insulina através da Triagem Técnica das Prescrições Médicas (TTPM), com o intuito de checar eventuais problemas que pudessem afetar a eficácia terapêutica. Metodologia: foi realizado um estudo transversal retrospectivo de 16 prescrições médicas, de 9 adultos com DM2 usuários de insulina. A TTPM foi realizada através da utilização do instrumento específico, elaborado para este fim, onde foram checados eventuais problemas que poderiam afetar a segurança da prescrição de insulina. Resultados e Discussões: foi observado que todas as receitas médicas apresentavam algum problema que pudesse afetar a segurança da prescrição. Dentre os problemas mais observados a falta de informações sobre o paciente (idade e/ou endereço) e o uso de abreviaturas foi encontrada em todas as prescrições. A omissão de dados sobre o prescritor, ou local onde trabalha, como endereço e/ou telefone foi encontrada em 94% das prescrições. Além do mais, 74% das prescrições foram consideradas ilegíveis ou pouco legíveis. Conclusão: os problemas observados envolvem a prescrição inadequada... (Resumo completo, clicar acesso eletrônico abaixo)

Iluminação de exteriores utilizando tecnologia LED: estudo de caso

This Graduate Work aims to exhibit the analyze of the applicability of LED technology in a real outdoor lighting situation, and this project’s illumination solution is compared with the initial proposal, the sodium vapor lamps. What is studied is an outdoor area, like the roads and parking lots of a plant. A light planning software is used for this comparison, and the results are displayed instantly, allowing rapid analysis of the project through a simulation. The medium illuminance is the same in both solutions for this case study and minimum rates set as standard were observed then the analysis are appropriate in this project. Various aspects are considered in order to obtain a comprehensive analysis of this project, such as economic, environmental and quality of lighting. LED technology in outdoor lighting is presented in its infancy, but promising. Solid state lamps show great advantages when compared with other technologies, such as discharge lamps, however there are some disadvantages tied to new products that are expected to be overcome with technological development and increased production

Proteção de sistemas eletricos industriais: relés e suas aplicações em subestações

Electrical installations in industries involving high currents and voltages considered. On the other side is the common appearance of electrical failures, caused by human error, defects in electrical equipment or electrical installation aging itself. These failures are varied, those with the highest rate of occurrence and cause much damage to electrical installations, are overcurrent and overvoltage. Therefore there is a need to project a system that can detect and minimize possible effects caused by faults in electrical installations industries. Protection systems in electric industries emerge as an alternative to control especially voltage and current magnitudes. Engineered based on the functions of the relays, protection systems are an indispensable tool for any industrial substation. But the project of such systems becomes increasingly more complex, due to technological development. Electrical equipment not develop at the same speed that the protective devices (relays), making it indispensable knowledge of integration of technologies

Carvão (Sporisorium scitamineum) e podridão-abacaxi (Ceratocystis paradoxa) em cana-de-açúcar (Saccharum spp.): uma revisão

Sugarcane (Saccharum sp.) is an important grass cultivated in tropical and subtropical regions of the world, such as in Br, Ind, and Ch, and has its biomass being raw material for production of sugar, fuel ethanol, and some other derivatives. Fungal diseases infect sugarcane fields worldwide, damaging crops and thus, causing great economic losses. Fungal specialized structures act during all Pathogen-Host Relationship Cycle (PHRC) (survival, dissemination, infection, colonization, and reproduction of pathogen), maintaining fungal populations in cultivation soil, infecting plants in following crops and vegetative propagation of sugarcane by infected seeds may allow fungal transportation into regions where diseases haven’t occured before. Biotechnological methods and approaches have significantly contributed to understanding of the relationship among parasite and host, as to diseases management (control, detection, and prevention). Some techniques have daily applications in Agriculture, while others are only used in research and to breeding of host resistant varieties. Among notable diseases, smut (Sporisorium scitamineum) and pineapple disease (Ceratocystis paradoxa) are important because they cause damage and losses in sugarcane regions, although there are different periods for each one to occur. This work aims to review the PHRC for each patosystem, the biotechnological methods and approaches and its perspectives in the study and management of these diseases. As environment is an important factor to the effectiveness of PHRC, one chapter is dedicated to Global Climate Change (GCC) and its possible influences over these diseases in a longterm period

Fragmento d'alma

Fragmento D' Alma is an artistic research that part of a poetic tale through an initial charge of dawn one fantastic universe where words have life and willingly. The work includes sculptural works in order to interpret through the use of allegory situations of the tale in the form of sculptural compositions considering one or more characters in the story. In the sculptural work, I tried figuring the relations tale as my interpretations and poetic, so I sought theoretical studies of allegory after noting my affinity with the theme to contribute to the relationship text and image set in my work. I also sought to understand the relationships that the text played with sculptural work, so the chances that I could address this through dialogue between text and image. The studies collected through surveys pointed allegory by João Adolfo Hansen, as well as in various artistic and visual references present in my repertoire, such as the Baroque sculpture of Bernini, the performances of Berna Reale and literature of Neil Gaiman, contributed in this research to the development of my creative process during the preparation of this research work of art, making the complexity of the relationship between textual production and poetic sculptural three-dimensional, also became a concern and an integral theme of my work and my reflections presented here. Therefore, I conclude based on my own experience in this work, the artistic research inspired allegory might contribute to the development of my creative process during the preparation of this artwork, besides assigning relations between textual production and poetic dimensional sculpture

Qualidade de vida e vulnerabilidade social na percepção do idoso

According to data from the World Health Organization, the older population will grow sixteenfold from 1950 to 2025 in comparison to a fivefold population growth in the same period, which is referred to by UNO as the “Ageing Era”. This places Brazil in the sixth position in the contingent of older individuals worldwide, with a number that is higher than 32 million. Considering how topics such as quality of life and social vulnerability are important in face of the growing older population, these topics must be furthered studied so that they can be understood as important variables for both better clinical practice and scientific research. To describe the social vulnerability and evaluate the quality of life of older individuals in a population hospitalized in the internal medicine ward of Bauru State Hospital. This is a descriptive qualitative study that was conducted by means of interviews and using Bardin’s discourse analysis. The inclusion criteria used in this study were: individuals at 60 years of age who were mentally capable of answering the proposed questions. Two categories concerning Quality of Life and Social Vulnerability emerged from the interviews. The following emerged from the theme Quality of Life: “Life as something important” and subcategories that involved feeling useful in society, having a supportive family, independence, optimism and joy and survival. Also, the following emerged from the theme Social Vulnerability: “Negative recognition of older individuals in society” and subcategories that involved lack of respect, functional disability, family indifference, housing-related insecurity, an inefficient health care system and loneliness. Quality of Life and Social Vulnerability are largely discussed themes in the present scenario. In this study, it was possible to perceive that the older population needs social support, effective public... (Complete abstract click electronic access below)

Estudo sobre uma turbina a gás instalada em uma refinaria de petróleo

The gas turbine (GT) is known to have: low cost of capital over the amount of energy, high flexibility, high reliability, short delivery time, commissioning and commercial operation at the beginning and quick departure. The gas turbine is also recognized for its superior environmental performance, manifested in air pollution containment and reducing greenhouse gases (Mahi, 1994). Gas turbines in simple cycle mode (SC) have long been used by utilities to limited power generation peak. In addition, manufacturing facilities use gas turbines for power generation units on site, often in combination with the process of heat production, such as hot water and steam process. In recent years, the performance of industrial gas turbines has been improved due to significant investments in research and development, in terms of fuel to electricity conversion efficiency, plant capacity, availability and reliability. The greater availability of energy resources such as natural gas (NG), the significant reduction of capital costs and the introduction of advanced cycles, have also been a success factor for the increased use of gas turbines to load applications base (Poulikas, 2004). Open Cycle Gas Turbine with a greater degree of heat to the atmosphere may alternatively be used to produce additional electricity using a steam cycle, or to compose a cogeneration process. The combined cycle (CC) uses the heat from the gas turbine exhaust gas to increase the power output and increase the overall efficiency of more than 50% second (Najjar, 2001). The initial discovery of these cycles in the commercial power generation market was possible due to the development of the gas turbine. Only from the 1970s that gas turbine inlet temperature and therefore the exhaust gas temperature was sufficiently high to allow a better efficiency in the combined cycle ... (Complete Abstract click electronic access below)

Nanoregulação: Avaliação dos cenários internacional e brasileiro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Aços avançados de alta resistência: avaliação da microestrutura e propriedades dos aços DP 600 E DP 780

This work aims to study the Dual-Phase 600 and 780 steels, which are part of technology development project materials for the automotive industry. It is worth underscoring the antagonistic properties as the Dual-Phase steel assemble, high mechanical strength and elongation due its microstructure, ferrite and martensite. These properties are obtained by a intercritical heat treatment which facilitates the formation of a hardness metastable microstructure shaped plates of low carbon steels. The applicability of Dual Phase steel in the structure of vehicles is huge and its production is already on a commercial scale, so the study and development of this material implies lower cost in automobile manufacturing processes. The dual phase steels DP600 and DP780 underwent tensile, hardness and metallographic analysis to evaluate and comparing its properties. The results indicate that the DP780 steel has higher strength and hardness than the DP600 steel and its microstructure consists of martensite higher fraction which accounts for the higher resistance and hardness. However, the DP600 has higher conformability to DP780 steel

Políticas industriais: definição e importância (ou não) de fundamentação teórica (parte 1)

O objetivo deste artigo é fundamentar teoricamente as políticas industriais, caracterizando as razões da preferência por um referencial teórico não equilibrista e nãomaximizador. Procura-se também definir de maneira eficaz estas políticas, destacando seus resultados positivos em termos de alguns parâmetros. Este artigo será publicado em duas partes. Nesta edição define-se política industrial, analisando-se também as razões da despreocupação com uma fundamentação mais rigorosa das políticas industriais. Em seguida, apresenta-se sucintamente uma teoria capaz de embasar estas políticas, ao mesmo tempo em que se analisa se tais teorias são importantes (ou não), em termos empíricos. No quarto item, que será publicado na próxima edição deste Boletim, discute-se a possível utilidade da política industrial, enfatizando falhas de mercado, bens públicos, mercados não-competitivos, externalidades e desenvolvimento tecnológico.

Políticas industriais: definição e importância (ou não) de fundamentação teórica (parte 2)

O objetivo deste artigo é, essencialmente, fundamentar teoricamente as políticas industriais, sobretudo mostrando as razões da preferência por um referencial teórico não equilibrista e não-maximizador. Busca-se também definir de maneira eficaz estas políticas, destacando seus resultados positivos - pelo menos potencialmente - em termos de alguns parâmetros (produção, eficiência, produtividade, renda, bem-estar, etc.). Para isso define-se política industrial, analisando-se também as razões da despreocupação com uma fundamentação mais rigorosa das políticas industriais. Em seguida, apresenta-se sucintamente se uma teoria capaz de embasar estas políticas, ao mesmo tempo em que se analisa se tais teorias são importantes (ou não), em termos empíricos. Nesta segunda parte discute-se a possível utilidade da política industrial, enfatizando falhas de mercado, bens públicos, mercados não-competitivos, externalidades e desenvolvimento tecnológico.

Metas de inflação no Brasil (1999-2012): uma análise institucional

The paper shows the advantages and handicaps of implementing an inflation target (IT) regime, from post-keynesian and institutional stances. It is post-keynesian as long as it does not perceive any benefit in the mainstream split between monetary and fiscal policies. And it is institutional insofar as it shows there are several ways of implementing a policy, such that the chosen one is determined by historical factors, as illustrated by the Brazilian case. Thus, one could even support IT policies if their targets were seen just as “focusing devices” guiding economic policies, but also regarding with due attention other targets, as, in the short run, output growth and employment and, in the long run, technology and human development. Therefore, an IT is not necessary, although it can be admitted, mainly if the target is hidden from the public, in order to increase the flexibility of the central bank.