33 resultados para Tecido adiposo Teses

em Universidade Federal do Rio Grande do Norte(UFRN)


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Conselho Nacional de Desenvolvimento Cientfico e Tecnolgico

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Obesity is a chronic metabolic disease characterized by adipose tissue formation excess leading to an increase in body fat mass, of multifactorial origin, produced mainly by poor eating habits combined with a sedentary lifestyle. Data consider obesity as a serious disease that affects the world's population, ranking fifth in death rates. Faced with this situation, individuals seek, increasingly, means to lose weight with less physical effort and food. In 2009 and 2010 the drug liraglutide was lauched in order to reduce weight in individuals with diabetes mellitus type 2, thus avoiding the emergence of other diseases. The aggravating factor is that obese nondiabetic individuals are making use of this substance, even if its use is not authorized by ANVISA (Brazilian Health Surveillance Agency). Thus the objective of this research is to evaluate the effect of liraglutide for muscle or fat tissues and biochemical parameters in Swiss mice submitted to cafeteria diet and physical activity. The study was approved by the Ethics Committee on Animal Use - CEUA (n003 Protocol / 2014). For this study 74 animals (Swiss mice) were used, divided as follows: in the initial phase of this study, we carried out a pilot study (n = 10) divided into a control group (PCON) (n = 5) and cafeteria group (PCAF) (n = 5), in order to evaluate a cafeteria diet which was both attractive to the animals and that could provide an increase in adipose tissue. After the induction of the diet, animals were euthanized and as a result, the animals in the PCAF group showed an intra-abdominal adiposity 0.74 0.05 g, taken as the parameter for increasing fat in animals. Subsequently the study base was conducted for this research where animals were used (n = 64) divided into 2 groups: the Cafeteria Study Base Group (EBCAF) divided as follows: cafeteria + exercise + liraglutide (CEL) (n = 8), cafeteria + exercise + saline (CES) (n = 8), cafeteria + liraglutide (CL) (n = 8) and cafeteria + saline (CS) (n = 8). The Chow Study Base group (EBR) was divided into: exercise + liraglutide (EL) (n = 8), exercise + saline + (ES) (n = 8), liraglutide (L) (n = 8) and saline solution (SS) (n = 8). All animals went through the submission process to the cafeteria diet, followed by exercise protocol through swimming and treatment with the test substance intraperitoneally (200 mg / mL / kg). After the treatments, the animals were euthanized and had the following parameters evaluated: the muscle tissue mass, adipose tissue mass and biochemical parameters. It was observed that the processing done with the exercise-associated liraglutide reduced adipose tissue mass significantly (0.32 0.05 g) compared to the saline group (0.53 0.07 g). There were no changes in the muscle tissue of the group which was treated and exercised (1.39 0.03 g) compared to the saline group (1.33 0.03 g). Regarding biochemical parameters it was evident that there were changes in these parameters. Interesting to note that, although blood glucose values have been changed, the animals did not become diabetic. Thus, it appears that physical activity together with liraglutide is eficcient to the loss of intraabdominal adipose tissue and the maintenance of lean body mass thereby generating a satisfactory result in the pursuit of quality of life and disease prevention.

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Obesity is a chronic metabolic disease characterized by adipose tissue formation excess leading to an increase in body fat mass, of multifactorial origin, produced mainly by poor eating habits combined with a sedentary lifestyle. Data consider obesity as a serious disease that affects the world's population, ranking fifth in death rates. Faced with this situation, individuals seek, increasingly, means to lose weight with less physical effort and food. In 2009 and 2010 the drug liraglutide was lauched in order to reduce weight in individuals with diabetes mellitus type 2, thus avoiding the emergence of other diseases. The aggravating factor is that obese nondiabetic individuals are making use of this substance, even if its use is not authorized by ANVISA (Brazilian Health Surveillance Agency). Thus the objective of this research is to evaluate the effect of liraglutide for muscle or fat tissues and biochemical parameters in Swiss mice submitted to cafeteria diet and physical activity. The study was approved by the Ethics Committee on Animal Use - CEUA (n003 Protocol / 2014). For this study 74 animals (Swiss mice) were used, divided as follows: in the initial phase of this study, we carried out a pilot study (n = 10) divided into a control group (PCON) (n = 5) and cafeteria group (PCAF) (n = 5), in order to evaluate a cafeteria diet which was both attractive to the animals and that could provide an increase in adipose tissue. After the induction of the diet, animals were euthanized and as a result, the animals in the PCAF group showed an intra-abdominal adiposity 0.74 0.05 g, taken as the parameter for increasing fat in animals. Subsequently the study base was conducted for this research where animals were used (n = 64) divided into 2 groups: the Cafeteria Study Base Group (EBCAF) divided as follows: cafeteria + exercise + liraglutide (CEL) (n = 8), cafeteria + exercise + saline (CES) (n = 8), cafeteria + liraglutide (CL) (n = 8) and cafeteria + saline (CS) (n = 8). The Chow Study Base group (EBR) was divided into: exercise + liraglutide (EL) (n = 8), exercise + saline + (ES) (n = 8), liraglutide (L) (n = 8) and saline solution (SS) (n = 8). All animals went through the submission process to the cafeteria diet, followed by exercise protocol through swimming and treatment with the test substance intraperitoneally (200 mg / mL / kg). After the treatments, the animals were euthanized and had the following parameters evaluated: the muscle tissue mass, adipose tissue mass and biochemical parameters. It was observed that the processing done with the exercise-associated liraglutide reduced adipose tissue mass significantly (0.32 0.05 g) compared to the saline group (0.53 0.07 g). There were no changes in the muscle tissue of the group which was treated and exercised (1.39 0.03 g) compared to the saline group (1.33 0.03 g). Regarding biochemical parameters it was evident that there were changes in these parameters. Interesting to note that, although blood glucose values have been changed, the animals did not become diabetic. Thus, it appears that physical activity together with liraglutide is eficcient to the loss of intraabdominal adipose tissue and the maintenance of lean body mass thereby generating a satisfactory result in the pursuit of quality of life and disease prevention.

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Micronutrient deficiencies affect individuals mainly in developing countries, where vitamin A deficiency is a public health problem worldwide more worrying, especially in groups with increased physiological needs such as children and women of reproductive age. Vitamin A is supplied to the body through diet and has an important role in the visual process, cell differentiation, maintenance of epithelial tissue, reproductive and resistance to infection. The literature has demonstrated the relationship between vitamin A and diabetes, including gestational, leading to a risk to both mother and child. Gestational diabetes is any decrease in glucose tolerance of variable magnitude diagnosed each the first time during pregnancy, and may or may not persist after delivery. Insulin resistance during pregnancy is associated with placental hormones, as well as excess fat. Studies have shown that retinol transport protein produced in adipose tissue in high concentrations, this would be associated with resistance by interfering with insulin signaling. Therefore, this study aimed to evaluate the concentration of retinol in serum and colostrum from healthy and diabetic mothers in the immediate postpartum period. One hundred and nine parturient women were recruited, representing seventy-three healthy and thirty-six diabetic. Retinol was extracted and subsequently analyzed by High Performance Liquid Chromatography. Among the results highlights the mothers with gestational diabetes were older than mothers healthy, had more children and a higher prevalence of cases of cesarean section. Fetal macrosomia was present in 1.4% of healthy parturient women and in 22.2% of diabetic mothers. The maternal serum retinol showed an average of 39.7 12.5 mg/dL for healthy parturients 35.12 15 mg/dL for diabetic and showed no statistical difference. It was observed that in the group of diabetic had 17% vitamin A deficiency, whereas in the healthy group, only 4% of the women were deficentes. Colostrum, the concentration of retinol in healthy was 131.3 56.2 mg/dL and 125.3 41.9 mg/dL in diabetic did not differ statistically. This concentration of retinol found in colostrum provides approximately 656.5 mg/day for infants born to healthy mothers and 626.5 mg/day for infants of diabetic mothers, based on a daily consumption of 500 mL of breast milk and need Vitamin A 400 mg/day, thus reaching the requirement of the infant. The diabetic mothers showed significant risk factors and complications related to gestational diabetes. Although no 11 difference was found in serum retinol concentration and colostrum among women with and without gestational diabetes, the individual analysis shows that parturients women with diabetes are 4.9 times more likely to develop vitamin A deficiency than healthy parturients. However, the supply of vitamin A to the newborn was not committed in the presence of gestational diabetes

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Mammography is a diagnostic imaging method in which interpretation depends on knowledge of radiological aspects as well as the clinical exam and pathophysiology of breast diseases. In this work a mammography phantom was developed to be used for training in the operation of mammographic x-ray equipment, image quality evaluation, self-examination and clinical examination of palpation. Polyurethane was used for the production of the phantoms for its physical and chemical properties and because it is one of the components normally used in prostheses. According to the range of flexibility of the polyurethane, it was possible to simulate breasts with higher or lower amount of adipose tissue. Pathologies such as areolar necrosis and tissue rejection due to surgery reconstruction after partial mastectomy were also simulated. Calcifications and nodules were simulated using the following materials: polyethylene, poly (methyl methacrylate), polyamide, polyurethane and poly (dimethyl silicone). Among these, polyethylene was able to simulate characteristics of calcification as well as breast nodules. The results from mammographic techniques used in this paper for the evaluation of the phantoms are in agreement with data found in the literature. The image analyses of four phantoms indicated significant similarities with the human skin texture and the female breast parenchyma. It was possible to detect in the radiographic images produced regions of high and low radiographic optical density, which are characteristic of breasts with regions of different amount of adipose tissue. The stiffnesses of breast phantoms were adjusted according to the formulation of the polyurethane which enabled the production of phantoms with distinct radiographic features and texture similar to human female breast parenchyma. Clinical palpation exam of the phantoms developed in this work indicated characteristics similar to human breast in skin texture, areolar region and parenchyma

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A Sndrome de Berardinelli-Seip ou Lipodistrofia Generalizada Congnita (LGC) uma doena rara, com transmisso autossmica recessiva, caracterizada principalmente pela ausncia quase total de tecido adiposo. Os pacientes afetados apresentam resistncia a insulina, dislipidemia e hipertenso arterial. Estudos tm evidenciado que estas alteraes metablicas interferem na modulao autonmica para o n sinusal. O principal objetivo deste estudo foi investigar a modulao autonmica em pacientes portadores de LGC, atravs da variabilidade de freqncia cardaca (VFC), pelo mtodo linear de domnio de tempo na Eletrocardiografia Dinmica de 24 horas e estabelecer um critrio relativamente simples, no invasivo, para diagnstico de disfuno autonmica nestes pacientes. Participou voluntariamente deste estudo transversal, um grupo de pacientes portadores de LGC (n=18) na faixa etria de 9,3 a 39,8 anos (21,3 8,3) cuja variabilidade de freqncia cardaca foi comparada com um grupo de pacientes controles (n=19) com idade de 9,3 a 39,1 anos (21,4 7,8). Todos os voluntrios foram submetidos avaliao clnica, laboratorial, antropomtrica e anlise de VFC no domnio de tempo atravs de eletrocardiografia dinmica de 24 horas. Para anlise dos dados relativos aos ndices temporais de VFC foram utilizados o MeanRR, SSDN e rMSSD. Pacientes com LGC apresentavam aumento da presso arterial comparados com indivduos do grupo controle (sistlica, 131,1 vs 106,3 mmHg, p<0,05); diastlica, 85,0 vs 68,2 mmHg, p<0,05) e 10 tinham critrios para diagnstico de Hipertenso Arterial e Hipertrofia do Ventrculo Esquerdo. Os nveis de glicose, triglicerdeos, colesterol e HOMA-R eram elevados e 12 pacientes tinham critrios para diabetes mellitus tipo 2. Comparado com os controles, pacientes com LGC tinham diminuio dos ndices MeanRR (639,8 vs 780,5 ms, p<0,001), SDNN (79,2 vs 168,5 ms, p<0,001), e rMSSD (15,8 vs 59,6 ms, p<0,001). Em pacientes com LGC, a reduo da VFC foi independente de distrbios metablicos e hemodinmicos. Os resultados de nosso experimento indicam que pacientes com LGC apresentavam modulao autonmica anormal caracterizada pelo aumento da freqncia cardaca e pronunciada reduo da VFC, independente de distrbios metablicos e hemodinmicos observados nesta sndrome. O carter multidisciplinar desse estudo fica contemplado pela interao de profissionais de diversas reas como: cardiologia, endocrinologia, metabolismo, neurologia, nutrio, etc

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Congenital generalized lipodystrophy is a rare genetic disease with autosomal recessive inheritance characterized by the generalized absence of subcutaneous adipose tissue and insulin resistance. The aim of our study was to determine the profile of patients with congenital generalized lipodystrophy (Berardinelli-Seip syndrome) through their clinical history, eating habits, and socioeconomic and cultural aspects; assess food consumption and nutritional status of the study group; propose and evaluate a diet therapy model associated to oral supplementation with zinc to help in the control and prevention of metabolic complications associated to the pathology. Initial assessment of food consumption indicated a voracious appetite in all the patients studied. The introduction of zinc reduced appetite, contributing to patient adherence to the food plan proposed. It was also observed that the proposed diet contributed mainly to glycidic control, specifically with respect to HbA1c. The nutritional status of the patients investigated was adequate in terms of body mass index (BMI), arm muscle circumference (AMC), arm muscle area AMA, but triceps skinfold (TSF) indicated serious malnutrition. Our study is unique in the literature and provides important information to the field of nutrition and to individuals with this pathology. Furthermore, it contemplates the interdisciplinary and multidisciplinary requirements of the Postgraduate Program in Health Sciences of the Federal University of Rio Grande do Norte (UFRN), Natal, Brazil

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A Sndrome de Berardinelli-Seip (SBS) um distrbio raro do metabolismo dos lipdios, caracterizada pela ausncia quase total de tecido adiposo subcutneo, hipertrigliceridemia, hipoleptinemia e diabetes insulino resistente ou lipoatrfico. Sua etiologia envolve implicaes hipotalmicas, alteraes nos receptores de insulina e mutaes nos genes AGPAT2, Gng3lg, CAV1 e PTRF. O tecido adiposo secreta diversas substncias, tais como: leptina, resistina, adiponectina, esterides, TNF , IL-6, PAI-1, angiotensinognio, IGF-1. Muitas delas esto associadas ao diabetes mellitus tipo 2, obesidade e hipertenso. Os PPARs so fatores transcricionais pertencentes superfamlia de receptores nucleares ligantes ativados. Sabe-se que o PPAR , importante para o metabolismo lipdico e glicdico e que o ligante natural do PPAR derivado do cido graxo. Nesse sentido, foram avaliados 24 pacientes portadores da SBS, provenientes do Estado do Rio Grande do Norte, com a mediana das idades de 18,5 anos (0,55 a 47 a), sendo 9 (37,5 %) do gnero masculino e 15 (62,5 %) do gnero feminino. Quanto ao grupo tnico, foram classificados em caucasides (brancos) 21 (87,5 %) e negrides 3 (12,5 %) pacientes. Foram feitas avaliaes clnico-endocrinolgica, bioqumica, hormonal, molecular e o estudo dos polimorfismos Adiponectina ADIPOQ, PPAR&#947;2 Pro12Ala, LPL-PvuII, APOC3-SstI e LDLR-AvaII em portadores da SBS. Nesta populao ns no encontramos nenhuma associao de parmetros lipdicos e glicdicos com os polimorfismos LPL-PvuII, APOC3-SstI e LDLR-AvaII. Porm, observamos associao entre Adiponectina ADIPOQ e PPAR&#947;2 Pro12Ala e nveis lipdicos mais elevados, sugerindo um papel biolgico para estes fatores, indicando estudos mais aprofundados

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Introduo: A obesidade infantil apresenta incidncia crescente e as possveis comorbidades, como alterao da funo respiratria, esto cada vez mais presente nessa faixa etria. O tecido adiposo impe carga ao sistema respiratrio o que leva a um padro restritivo. Essa condio sofre alteraes com as mudanas posturais, onde a gravidade influencia o padro respiratrio de acordo com o posicionamento adotado. Objetivo: Avaliar a distribuio dos volumes total e regional e o movimento tracoabdominal de crianas e adolescentes que esto acima do peso nas posturas supino e sentado. Mtodos: Cinqenta e duas crianas/adolescentes (8-12 anos) divididas em trs grupos: Grupo Obeso (GO=22); Grupo Sobrepeso (GSP=9); Grupo Controle (GC=21) foram avaliadas quanto s medidas antropomtricas, teste de funo pulmonar, exame das presses respiratrias mxima e a pletismografia optoeletrnica em duas posturas, supino e sentado, durante a respirao tranquila. Resultados: As crianas que esto obesas apresentaram maiores valores em relao ao GSP e GC das seguintes variveis espiromtricas: volume expiratrio forado no primeiro segundo (VEF1) (p<0.05) e capacidade vital forada (CVF) (p<0.01). No exame de manovacuometria o GO apresentou um aumento na presso inspiratria mxima (PImx) (p<0.01) em comparao com os outros grupos. Quanto distribuio do volume corrente, o GO possui uma maior contribuio do compartimento abdominal (AB) na postura supina (p<0.05) em relao ao GC e GSP, enquanto que na postura sentada os grupos no diferiram em relao distribuio dos volumes. O GO apresentou maior assincronia na postura supina (p<0.05) e maior velocidade de encurtamento (p<0.05) em relao os outros grupos. Concluso: A obesidade em crianas/adolescentes no provoca prejuzos na funo pulmonar, incrementa a fora muscular inspiratria, aumenta a participao do compartimento AB e a assincronia no MTA na postura em supino, conclui-se que a postura supina associada obesidade provoca aumento da sobrecarga do diafragma, desfavorecendo o desempenho do sistema respiratrio.

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Micronutrient deficiencies affect individuals mainly in developing countries, where vitamin A deficiency is a public health problem worldwide more worrying, especially in groups with increased physiological needs such as children and women of reproductive age. Vitamin A is supplied to the body through diet and has an important role in the visual process, cell differentiation, maintenance of epithelial tissue, reproductive and resistance to infection. The literature has demonstrated the relationship between vitamin A and diabetes, including gestational, leading to a risk to both mother and child. Gestational diabetes is any decrease in glucose tolerance of variable magnitude diagnosed each the first time during pregnancy, and may or may not persist after delivery. Insulin resistance during pregnancy is associated with placental hormones, as well as excess fat. Studies have shown that retinol transport protein produced in adipose tissue in high concentrations, this would be associated with resistance by interfering with insulin signaling. Therefore, this study aimed to evaluate the concentration of retinol in serum and colostrum from healthy and diabetic mothers in the immediate postpartum period. One hundred and nine parturient women were recruited, representing seventy-three healthy and thirty-six diabetic. Retinol was extracted and subsequently analyzed by High Performance Liquid Chromatography. Among the results highlights the mothers with gestational diabetes were older than mothers healthy, had more children and a higher prevalence of cases of cesarean section. Fetal macrosomia was present in 1.4% of healthy parturient women and in 22.2% of diabetic mothers. The maternal serum retinol showed an average of 39.7 12.5 mg/dL for healthy parturients 35.12 15 mg/dL for diabetic and showed no statistical difference. It was observed that in the group of diabetic had 17% vitamin A deficiency, whereas in the healthy group, only 4% of the women were deficentes. Colostrum, the concentration of retinol in healthy was 131.3 56.2 mg/dL and 125.3 41.9 mg/dL in diabetic did not differ statistically. This concentration of retinol found in colostrum provides approximately 656.5 mg/day for infants born to healthy mothers and 626.5 mg/day for infants of diabetic mothers, based on a daily consumption of 500 mL of breast milk and need Vitamin A 400 mg/day, thus reaching the requirement of the infant. The diabetic mothers showed significant risk factors and complications related to gestational diabetes. Although no 11 difference was found in serum retinol concentration and colostrum among women with and without gestational diabetes, the individual analysis shows that parturients women with diabetes are 4.9 times more likely to develop vitamin A deficiency than healthy parturients. However, the supply of vitamin A to the newborn was not committed in the presence of gestational diabetes

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Mammography is a diagnostic imaging method in which interpretation depends on knowledge of radiological aspects as well as the clinical exam and pathophysiology of breast diseases. In this work a mammography phantom was developed to be used for training in the operation of mammographic x-ray equipment, image quality evaluation, self-examination and clinical examination of palpation. Polyurethane was used for the production of the phantoms for its physical and chemical properties and because it is one of the components normally used in prostheses. According to the range of flexibility of the polyurethane, it was possible to simulate breasts with higher or lower amount of adipose tissue. Pathologies such as areolar necrosis and tissue rejection due to surgery reconstruction after partial mastectomy were also simulated. Calcifications and nodules were simulated using the following materials: polyethylene, poly (methyl methacrylate), polyamide, polyurethane and poly (dimethyl silicone). Among these, polyethylene was able to simulate characteristics of calcification as well as breast nodules. The results from mammographic techniques used in this paper for the evaluation of the phantoms are in agreement with data found in the literature. The image analyses of four phantoms indicated significant similarities with the human skin texture and the female breast parenchyma. It was possible to detect in the radiographic images produced regions of high and low radiographic optical density, which are characteristic of breasts with regions of different amount of adipose tissue. The stiffnesses of breast phantoms were adjusted according to the formulation of the polyurethane which enabled the production of phantoms with distinct radiographic features and texture similar to human female breast parenchyma. Clinical palpation exam of the phantoms developed in this work indicated characteristics similar to human breast in skin texture, areolar region and parenchyma

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VITULLO, Nadia Aurora Vanti. Avaliao do banco de dissertaes e teses da Associao Brasileira de Antropologia: uma anlise cienciomtrica. 2001. 143 f. Dissertaao (Mestrado) - Curso de Mestrado em Biblioteconomia e Cincia da Informao, Pontifcia Universidade Catlica de Campinas, Campinas, 2001.

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OLIVEIRA, Raimundo Muniz de. Biblioteca digital de teses e dissertaoes: uma referencia fundamental. In: CINFORM ENCONTRO NACIONAL DE ENSINO E PESQUISA DA INFORMAAO,HUMANISMO E DESENVOLVIMENTO CIENTIFICO E TECNOLOGICO,7.,2007,Salvador. Anais...Salvador:UFBA, 2007.Disponivel em:www.cinform.ufba.br>. Acesso em: 27 set. 2007. Acesso em: 27 set. 2010.

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CUNHA, Jacqueline de Arajo. Biblioteca Digital de Teses e Dissertaes: uma estratgia de preservao da memria cientfica. 2009. 141f. Dissertao (Mestrado)- Programa de Ps-Graduao em Cincia da Informao. Universidade Federal da Paraba, Joo Pessoa, 2009.

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A pesquisa aborda o uso das Tecnologias de Informao e Comunicao, que vem revolucionando as atividades e ocasionando muitas mudanas relacionadas ao acesso e uso de informaes. O objetivo foi analisar o grau de utilizao do conhecimento cientfico produzido pelos Programas de Ps-Graduao das Universidades Pblicas Brasileiras, atravs da BDTD, pelos mestrandos dos referidos programas. Nos procedimentos metodolgicos realizados, procurou-se inicialmente analisar o amplo espectro da populao do corpus da pesquisa. Em razo da impossibilidade de trabalhar com os Programas de Ps-Graduao como um todo, optou-se por fazer um recorte, elegendo os cursos de Ps-Graduao em Cincia da Informao, vez que estes representam o principal segmento social de interesse da pesquisa. Foi utilizado o mtodo de estudo de usurios, onde se optou por adotar o grupo, estudos orientados aos usurios, que identifica as necessidades e comportamento de acesso e uso da informao. Para coletar os dados, elaborou-se um questionrio semi-estruturado com 25 questes, que versavam sobre o uso, dificuldades de acesso e recuperao da informao, bem como a satisfao na utilizao dessa fonte informacional. Dentre os vrios resultados obtidos, podemos destacar o fato de que a maioria dos mestrandos (71,8%) s teve contato com a BDTD somente no momento em que se encontrava cursando o mestrado e, somente 24,3%, tiveram contato durante a graduao. Estes resultados representam um problema, que pode afetar o bom desempenho do projeto BDTD, o qual consiste em disseminar e divulgar a produo cientfica dos Programas de Ps-Graduao das Universidades Pblicas Brasileiras para a sua comunidade. Foi observado tambm, que os mestrandos oriundos do curso de Biblioteconomia tende a ter contato com a BDTD bem mais cedo do que mestrandos de outros cursos de graduao. A fim de minimizar o problema detectado, prope-se uma divulgao mais eficaz na graduao atravs de dois procedimentos: Primeiro, o docente deve fazer uma divulgao mais eficaz da BDTD junto aos discentes de todos os cursos de graduao; segundo: dever ser feita a divulgao na mdia eletrnica, atravs da insero de cones da BDTD, nos portais dos Departamentos dos Cursos de Graduao das Universidades Pblicas Brasileiras. Acredita-se que com estes procedimentos seja possvel aperfeioar o uso dessa fonte de informao cientfica.