Distinct disulfide isomers of μ-Conotoxins KIIIA and KIIIB block voltage-gated sodium channels

In the preparation of synthetic conotoxins containing multiple disulfide bonds, oxidative folding can produce numerous permutations of disulfide bond connectivities. Establishing the native disulfide connectivities thus presents a significant challenge when the venom-derived peptide is not available, as is increasingly the case when conotoxins are identified from cDNA sequences. Here, we investigate the disulfide connectivity of μ-conotoxin KIIIA, which was predicted originally to have a [C1–C9,C2–C15,C4–C16] disulfide pattern based on homology with closely related μ-conotoxins. The two major isomers of synthetic μ-KIIIA formed during oxidative folding were purified and their disulfide connectivities mapped by direct mass spectrometric collision-induced dissociation fragmentation of the disulfide-bonded polypeptides. Our results show that the major oxidative folding product adopts a [C1–C15,C2–C9,C4–C16] disulfide connectivity, while the minor product adopts a [C1–C16,C2–C9,C4–C15] connectivity. Both of these peptides were potent blockers of NaV1.2 (Kd values of 5 and 230 nM, respectively). The solution structure for μ-KIIIA based on nuclear magnetic resonance data was recalculated with the [C1–C15,C2–C9,C4–C16] disulfide pattern; its structure was very similar to the μ-KIIIA structure calculated with the incorrect [C1–C9,C2–C15,C4–C16] disulfide pattern, with an α-helix spanning residues 7–12. In addition, the major folding isomers of μ-KIIIB, an N-terminally extended isoform of μ-KIIIA identified from its cDNA sequence, were isolated. These folding products had the same disulfide connectivities as μ-KIIIA, and both blocked NaV1.2 (Kd values of 470 and 26 nM, respectively). Our results establish that the preferred disulfide pattern of synthetic μ-KIIIA and μ-KIIIB folded in vitro is 1–5/2–4/3–6 but that other disulfide isomers are also potent sodium channel blockers. These findings raise questions about the disulfide pattern(s) of μ-KIIIA in the venom of Conus kinoshitai; indeed, the presence of multiple disulfide isomers in the venom could provide a means of further expanding the snail’s repertoire of active peptides.

An effective VAR planning to improve dynamic voltage profile of distribution networks with distributed wind generation

This paper proposes an effective VAR planning based on reactive power margin for the enhancement of dynamic voltage stability in distribution networks with distributed wind generation. The analysis is carried over a distribution test system representative of the Kumamoto area in Japan. The detailed mathematical modeling of the system is also presented. Firstly, this paper provides simulation results showing the effects of composite load on voltage dynamics in the distribution network through an accurate time-domain analysis. Then, a cost-effective combination of shunt capacitor bank and distribution static synchronous compensator (D-STATCOM) is selected to ensure fast voltage recovery after a sudden disturbance. The analysis shows that the proposed approach can reduce the size of compensating devices, which in turn, reduces the cost. It also reduces power loss of the system.

Voltage profile improvement for distributed wind generation using D-STATCOM

This paper presents the application of FACTS devices for the enhancement of dynamic voltage stability in distribution networks with distributed wind generation. The analysis is carried over a test distribution system representative of the Kumamoto area in Japan. The detailed mathematical modelling of the system is also presented. Firstly, this paper provides simulation results showing the effects of higher and lower penetration of distributed wind generation on the voltage dynamics in a faulted system. Then, a distribution static synchronous compensator (D-STATCOM) is used to improve the voltage profile of the system. This analysis shows that D-STATCOM has significant performance to improve the voltage dynamics of distribution system compared to shunt capacitor.

Voltage stability analysis with optimum size and location based synchronous machine DG

Power loss of a distribution system can be reduced significantly by using optimum size and location of distributed generation (DG). Proper allocation of DG with appropriate size maximizes overall system efficiency. Moreover it improves the reliability and voltage profile of the distribution system. In this paper, IEEE 123 node test feeder has been considered to determine the optimum size and location of a synchronous machine based DG for loss reduction of the system. This paper also investigates the steady-state and dynamic voltage profile of that three phase unbalance distribution network in presence of DG with optimum size. This analysis shows that optimum size of DG at proper location minimizes the power loss as well as improves the dynamic voltage profile of the distribution system.

Voltage oscillations in power distribution networks in the presence of DFIGs and induction motor loads

This paper investigates the oscillatory behavior of power distribution systems in the presence of distributed generation. The analysis is carried out over a distribution test system with two doubly fed induction type wind generators and different types of induction motor loads. The system is linearized by the perturbation method. Eigenvalues are calculated to see the modal interaction within the system. The study indicates that interactions between closely placed converter controllers and induction motor loads significantly influence the damping of the oscillatory modes of the system. The critical modes have a frequency of oscillation between the electromechanical and subsynchronous oscillations of power systems. Time-domain simulations are carried out to verify the validity of the modal analysis and to provide a physical feel for the types of oscillations that occur in distribution systems. Finally, significant parameters of the system that affect the damping and frequency of the oscillation are identified.

Impact of high wind penetration on the voltage profile of distribution systems

In this paper, simulation results showing the effect of lower and higher penetration of distributed wind generation on the voltage profile in distribution systems have been presented. The analysis is carried out over two distribution test systems. The detailed mathematical modeling of the system is also presented. It also investigates the small-signal stability of distribution systems using eigenvalue approach. The analyses show that voltage variation problems occur in different nodes of the distribution networks with an increase of penetration level. However, proper selection of dispersion level can improve the voltage profile of the distribution systems

Enhancement of transient stability limit and voltage regulation with dynamic loads using robust excitation control

In stressed power systems with large induction machine component, there exist undamped electromechanical modes and unstable montonic voltage modes. This article proposes a sequential design of an excitation controller and a power system stabiliser (PSS) to stabilise the system. The operating region, with induction machines in stressed power systems, is often not captured using a linearisation around an operating point, and to alleviate this situation a robust controller is designed which guaruntees stable operation in a large region of operation. A minimax linear quadratic Gaussian design is used for the design of the supplementary control to automatic voltage regulators, and a classical PSS structure is used to damp electromechanical oscillations. The novelty of this work is in proposing a method to capture the unmodelled nonlinear dynamics as uncertainty in the design of the robust controller. Tight bounds on the uncertainty are obtained using this method which enables high-performance controllers. An IEEE benchmark test system has been used to demonstrate the performance of the designed controller

Integration of roof-top solar photovoltaic systems into the low voltage distribution network

The advancement in solar photovoltaic (PV) technology, the cost and efficiency of PVs have encouraged users worldwide to adopt more and more PVs as it is free from greenhouse gas emissions and unlimited in nature. Integration of roof-top solar PV systems is currently emerging rapidly in Australia as the governments are giving attractive incentives and encouraging households to build a sustainable climate-friendly society for the future. The key major barriers to the integration of roof-top solar PV systems are the uncertainties in the performance of the low voltage distribution network due to the intermittent nature of solar PV sources. In this paper, a model was developed to investigate the potential technical impacts of integrating roof-top solar PV systems into the low voltage distribution network in a subtropical climate. The results show that integration of roof-top solar PV in the customer premises causes uncertainties such as voltage fluctuations, phase unbalance, distribution transformer overloading, reactive power compensation, and harmonic injections that detract the overall power quality of the typical distribution network. © 2014 AIP Publishing LLC.

Research of a new type rectifier and voltage-multiplier piezoelectric energy harvester circuit

Traditional rectifier circuit can convert AC to DC, but some disadvantages can't be avoided, such as small output current, high power consumption, low conversion efficiency. This paper designs a new type of rectifier voltage-multiplier circuit named MR MOS circuit. It uses a low let-through resistance MOS tube to replace the conventional rectifier diode, and adds the voltage-multiplying factor to the synchronous input port. Therefore, it can improve the rectifier effect and increase the output voltage. By the simulation result of Synopsys Saber Platform, it shows that the new type circuit can implement the rectification and voltage-multiplying by the simulating output pulse voltage of nano fiber made in Deakin University as the source of excitation. It can provide the basic theoretical of the piezoelectric energy harvester (PEH) development, and has certain reference significance to the development of piezoelectricity technology. © (2014) Trans Tech Publications.

Development of a low actuation voltage electrostatic RF MEMS switch

The research focused on the design, fabrication and measurement of a low actuation voltage micro electro mechanical high frequency switch. The fabricated micro switch offers outstanding radio frequency parameters for a very large frequency band, with actuation voltage and switching time less than 20 volts and 3 micro seconds, respectively.