Controlling Access to Personal Information

Tässä diplomityössä käsitellään henkilökohtaisen tiedon saannin kontrollointia ja tiedon kuvaamista. Työn käytännön osuudessa suunniteltiin XML –malli henkilökohtaisen tiedon kuvaamiseen. Henkilökohtaisten tietojen käyttäminen mahdollistaa henkilökohtaisen palvelun tarjoamisen ja myös palvelun automatisoinnin käyttäjälle. Henkilökohtaisen tiedon kuvaaminen on hyvin oleellista, jotta palvelut voivat kysellä ja ymmärtää tietoja. Henkilökohtaiseen tietoon vaikuttaa erilaisia tekijöitä, jotka on myös otettava huomioon tietoa kuvattaessa. Henkilökohtaisen tiedon leviäminen eri palveluiden tarjoajille tuo mukanaan myös riskejä. Henkilökohtaisen tiedon joutuminen väärän henkilön käsiin saattaa aiheuttaa vakaviakin ongelmia tiedon omistajalle. Henkilökohtaisen tiedon turvallisen ja luotettavan käytettävyyden kannalta onkin hyvin oleellista, että käyttäjällä on mahdollisuus kontrolloida kenelle hän haluaa luovuttaa mitäkin tietoa.

Evolution of Bordetella pertussis post vaccination

Evolution of Bordetella pertussis post vaccination Whooping cough or pertussis is caused by the gram-negative bacterium Bordetella pertussis. It is a highly contiguous disease in the human respiratory tract. Characteristic of pertussis is a paroxysmal cough with whooping sound during gasps of breath after coughing episodes. It is potentially fatal to unvaccinated infants. The best approach to fight pertussis is to vaccinate. Vaccinations against pertussis have been available from the 1940s. Traditionally vaccines were whole-cell pertussis (wP) preparations as part of the combined diphtheria-tetanus-pertussis (DTP) vaccines. More recently acellular pertussis (aP) vaccines have replaced the wP vaccines in many countries. The aP vaccines are less reactogenic and can also be administered to school children and adults. There are several publications reporting variation in the i>B. pertussis virulence factors that are also aP vaccine antigens. This has occurred in the genes coding for pertussis toxin and pertactin about 15 to 30 years after the introduction of pertussis vaccines to immunisation programs. Resurgence of pertussis has also been reported in many countries with high vaccination coverage. In this study the evolution of B. pertussis was investigated in Finland, the United Kingdom, Poland, Serbia, China, Senegal and Kenya. These represent countries with a long history of high vaccination coverage with stable vaccines or changes in the vaccine formulation; countries which established high vaccination coverage late; and countries where vaccinations against pertussis were started late. With bacterial cytotoxicity and cytokine measurements, comparative genomic hybridisation, pulsed-field gel electrophoresis (PFGE), genotyping and serotyping it was found that changes in the vaccine composition can postpone the emergence of antigenic variants. It seems that the change in PFGE profiles and the loss of genetic material in the genome of B. pertussis are similar in most countries and the vaccine-induced immunity is selecting non-vaccine type strains. However, the differences in the formulation of the vaccines, the vaccination programs and in the coverage of pertussis vaccination have affected the speed and timing of these changes.

Die Beendigung von finnischen und deutschen Telefonaten. Eine interaktionslinguistische, kontrastierende Untersuchung

Suomalaisten ja saksalaisten arkikeskustelujen välillä on sekä yhtäläisyyksiä että eroja. Tässä saksalaisen filologian alaan kuuluvassa tutkimuksessa tarkastellaan yhtä keskeistä arkikeskustelun toimintoa, puhelinkeskustelun lopetusta, suomen- ja saksanpuhujien tuottamana. Aineistona on käytetty suomen- ja saksankielisten äidinkielisten puhujien tätä tutkimusta varten nauhoittamia henkilökohtaisia luonnollisia puhelinkeskusteluja. Aineistoon valikoitui 12 suomalaista ja 12 saksalaista puhelua. Nauhoitteiden käyttöön on saatu asianmukainen lupa kaikilta osapuolilta. Puhelut on litteroitu saksalaisella kielialueella vakiintuneen GAT-litterointisysteemin mukaan. Teoreettis-metodisena kehyksenä on kaksi tutkimusalaa, vuorovaikutuslingvistiikka ja kielten vertailu. Vuorovaikutuslingvistinen tarkastelu keskittyy havaintoihin vuorojen ja puheen sekvenssien rakenteesta. Vuorojen merkitysten tulkinnassa hyödynnetään systemaattisesti prosodian antamia vihjeitä. Tuloksena on yksittäisten lopetusten keskustelunanalyyttinen lähikuvaus, jonka pohjalta määritellään kulloisenkin lopetuksen sekvenssirakenne. Kaikki lopetukset olivat siltä osin yhteneväisiä, että niissä kaikissa havaittiin ainakin aloittava, tulevaan tapaamiseen viittaava sekä lopputervehdyksiin johtava sekvenssi. Sekvenssirakenteen variaatioiden pohjalta aineiston lopetukset voidaan kuitenkin jaotella ryhmiin. Sekä suomen- että saksankielisessä aineistossa havaittiin kolmentyyppisiä lopetuksia: kompakteja, komplekseja ja keskeytettyjä lopetuksia. Ryhmittely kolmeen tyyppiin on avuksi seuraavassa kuvausvaiheessa, jossa verrataan suomen- ja saksankielisiä lopetuksia toisiinsa. Samanaikaisesti kun tutkimus valottaa kohtia, joissa kaksi aineistosettiä yhtenevät ja eroavat, se myös esittää, mitkä vuorovaikutuksen tasot soveltuvat kieltenvälisen vertailun kohteiksi. Pohdintaa siitä, mitä vuorovaikutuksen tasoja kieltenväliseen vertailuun voidaan sisällyttää, onkin toistaiseksi esitetty verrattain vähän. Työ siis rakentaa siltaa vuorovaikutuslingvistisen ja kontrastiivisen kielitieteen välille.

Development of a Glycocluster Adjuvant Mimicking Natural Yeast beta-(1,2)-Structures

Allergy is characterized by T helper (Th) 2-type immune response after encounter with an allergen leading to subsequent immunoglobulin (Ig) E-mediated hypersensitivity reaction and further allergic inflammation. Allergen-specific immunotherapy (SIT) balances the Th2-biased immunity towards Th1 and T regulatory responses. Adjuvants are used in allergen preparations to intensify and modify SIT. β-(1,2)-oligomannoside constituents present in Candida albicans (C. albicans) cell wall possess Th1-type immunostimulatory properties. The aim of this thesis was to develop a β-(1,2)-linked carbohydrate compound with known structure and anti-allergic properties to be applied as an adjuvant in SIT. First the immunostimulatory properties of various fungal extracts were studied. C. albicans appeared to be the most promising Th1-inducing extract, which led to the synthesis of various mono- or divalent oligomannosides designed on the basis of C. albicans. These carbohydrates did not induce strong cytokine production in human peripheral blood mononuclear cell (PBMC) cultures. In contrast to earlier reports using native oligosaccharides from C. albicans, synthetic -(1,2)-linked mannotetraose did not induce any tumor necrosis factor production in murine macrophages. Next, similarities with synthesized divalent mannosides and the antigenic epitopes of β-(1,2)-linked C. albicans mannan were investigated. Two divalent compounds inhibited specific IgG antibodies binding to below 3 kDa hydrolyzed mannan down to the level of 30–50% showing similar antigenicity to C. albicans. Immunomodulatory properties of synthesized carbohydrate assemblies ranging from mono- to pentavalent were evaluated. A trivalent acetylated dimannose (TADM) induced interleukin-10 (IL-10) and interferon-γ responses. TADM also suppressed birch pollen induced IL-4 and IL-5 responses in allergen (Bet v) stimulated PBMCs of birch pollen allergic subjects. This suppression was stronger with TADM than with other used adjuvants, immunostimulatory oligonucleotides and monophosphoryl lipid A. In a murine model of asthma, the allergen induced inflammatory responses could also be suppressed by TADM on cytokine and antibody levels.

From genotype to phenotype : diversity and population structure of Flavobacterium psychrophilum

Flavobacterium psychrophilum is the etiological agent of bacterial cold-water disease (BCWD) causing high fish mortalities and significant economic losses to the freshwater salmonid aquaculture industry around the world. Today BCWD outbreaks are mainly treated with environmentally hazardous antimicrobial agents and alternative preventative measures are urgently needed in order to ensure the well-being of animals and the sustainability of aquaculture. The diversity of pathogenic bacteria challenges the development of universal control strategies and in many cases the pathogen population structure, i.e. the total genetic diversity of the species must be taken into account. This work integrates the tools of modern molecular biology and conventional phenotypic microbiology to gain knowledge about the diversity and population structure of F. psychrophilum. The present work includes genetic characterization of a large collection of isolates collected from diverse origins and years, from aquaculture in a whole region including different countries, and provides the first international validation of a universal multilocus sequence typing (MLST) approach for unambiguous genetic typing of F. psychrophilum. Population structure analyses showed that the global F. psychrophilum population is subdivided into pathogenic species-specific clones, of which one particular genetic lineage, clonal complex CC-ST2, has been responsible for the majority of BCWD outbreaks in rainbow trout (Oncorhynchus mykiss) in European aquaculture facilities over several decades. Genotypic and phenotypic population heterogeneity affecting antimicrobial resistance in F. psychrophilum within BCWD outbreaks was discovered. Specific genotypes were associated with severe infections in farmed rainbow trout and Atlantic salmon (Salmo salar), and in addition to high adherence, antimicrobial resistance was strongly associated with outbreak strains. The study brought additional support for the hypothesis of an epidemic F. psychrophilum population structure, where recombination is an important force for the generation and maintenance of genetic diversity, and a significant contribution towards mapping the genetic diversity of this important fish pathogen. Evidence indicating dissemination of virulent strains with commercial movement of fish and fish products was strengthened.

Detection, Identification and Molecular Variation of Human Enteroviruses

Picornaviruses are the most common human viruses and the identification of the picornaviruses is nowadays based on molecular techniques, for example, reverse transcriptase polymerase chain reaction (RT-PCR). One aim of this thesis was to improve the identification of picornaviruses, especially rhino- and enteroviruses, with a real-time assay format and, also, to improve the differentiation of the viruses with genus-specific locked nucleic acid (LNA) probes. Another aim was to identify and study the causative agent of the enterovirus epidemics that appeared in Finland during seasons 2008-2010. In this thesis, the first version of picornavirus qRT-PCR with a melting curve analysis was used in a study of rhinovirus transmission within families with a rhinovirus positive index child where rhinovirus infection was monitored in all family members. In conclusion, rhinoviruses spread effectively within families causing mostly symptomatic infections in children and asymptomatic infections in adults. To improve the differentiation between rhino- and enterovirus the picornavirus qRT-PCR was modified with LNA-incorporated probes. The LNA probes were validated with picornavirus prototypes and different clinical specimen types. The LNA probe-based picornavirus qRT-PCR was able to differentiate all rhino- and enteroviruses correctly, which makes it suitable for diagnostic use. Moreover, in this thesis enterovirus outbreaks were studied with a well-observed method to create a strain-specific qRT-PCR from the typing region VP1 protein. In a hand-foot-and-mouth-disease (HFMD) outbreak in 2008, the causative agent was identified as CV-A6 and when the molecular evolution of the new HFMD CV-A6 strain was studied it was found that CV-A6 was the emerging agent for HFMD and onychomadesis. Furthermore, unusual E-30 meningitis epidemics that apeared during seasons 2009 and 2010 were studied with strain-specific qRT-PCR. The E-30 affected mostly adolescents and was probably spread in sports teams.